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Sawarni Hasibuan
Universitas Mercu Buana
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Karakteristik Fisikokimia dan Antibakteri Hasil Purifikasi Minyak Biji Nyamplung (Calophyllum inophyllum L.) Sawarni Hasibuan; Sahirman Sahirman; Ni Made Ayu Yudawati
agriTECH Vol 33, No 3 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.533 KB) | DOI: 10.22146/agritech.9553

Abstract

The high solid fraction in Calophyllum seed oil causes Calophyllum biodiesel-oil produced has a high viscosity, high acid value, high cloud point, and other negative influences that is difficult to meet the biodiesel standard SNI 04-7182-2006. This underlies the need to increase the added value of solid fraction Calophyllum seed oil, such as healthy soap. This research was aimed to purification, fractionation, and characterization of fatty acid component as well as the antibacterial and antioxidant owned. So that Calophyllum seed oil may be considered for healthy soap application. The design used in the purification process is factorial completely randomized design, consisting of three factors, namely acid phosphate concentration, temperature and heating time. Based on the research, it resulted in the best treatment for Calophyllum seed oil’s purification was reached by using 0,2% H3PO4  20% at 80  C for 15 minutes. Major fatty acid component in Calophyllum seed oil purification results are palmitic acid, stearic acid, oleic acid and linoleic acid that reached 98,19 percent of the total fatty acids. The results showed that the Calophyllum oil contains components steroids, flavonoids, saponins, and triterpenoids and has an antibacterial effect against Staphylococcus aureus but instead to Escherichia coli.ABSTRAKTingginya fraksi padat pada minyak biji nyamplung menyebabkan biodiesel nyamplung yang dihasilkan memilikiviskositas tinggi, bilangan asam tinggi, titik kabut tinggi, dan pengaruh buruk lainnya sehingga sulit memenuhi standar biodiesel SNI 04-7182-2006. Hal ini mendasari perlunya peningkatan nilai tambah fraksi padat minyak biji nyamplung. Tujuan penelitian adalah melakukan purifikasi, fraksinasi, dan karakterisasi minyak biji nyamplung. Karakterisasi dilakukan terhadap komponen asam lemak serta uji pengenalan bioantibakteri dan bioantioksidan minyak biji nyamplung untuk aplikasi sabun kesehatan. Rancangan yang digunakan pada proses purifikasi nyamplung adalah Rancangan Acak Lengkap Faktorial dengan tiga faktor yaitu konsentrasi asam fosfat 20%, suhu, dan lama pemanasan. Berdasarkan hasil penelitian, perlakuan terbaik purifikasi diperoleh pada penggunaan H3PO4 20% sebanyak 0,2% pada suhu 80 oC selama 15 menit. Komponen asam lemak utama pada minyak nyamplung hasil purifikasi adalah asam palmitat, asam stearat, asam oleat dan asam linoleat dengan total asam lemak mencapai 98,19 persen dari total asam lemak penyusun minyak nyamplung. Hasil penelitian menunjukkan bahwa minyak nyamplung mengandung komponen steroid, flavonoid, saponin, dan triterpenoid serta memiliki efek antibakteri terhadap Staphylococcus aureus namun sebaliknya bagi Escheria coli.
Co-Authors Adi Permana Adriana Sari Aryani Ahmad Tanzil Daulay Alam, Fachrul Aljabar, Muhammad Wahid Malik Alloysius Vendhi Prasmoro, Alloysius Vendhi Amar Ma'ruf Amar Ma'ruf Anggara, Hafidz Armenia, Ivan Gustra Manca Bambang Wahyudiono Banuwati, Ninis Bayu Hari Nugroho Bayu Hari Nugroho Bayu Irwanto Bismar Maulani Choesnul Jaqin Citra, Zel Daruki Daruki Daruki, D Deky Aryanto Dewi Nusraningrum, Dewi Dian Widi Astuti, Budi Irawan Prima Putra, Dian Widi Astuti, E.Gumbira Said Edi Djatmika Endang Heriansyah Erwin Ramdhanu Kurniawan Fachrul Alam Fatahillah, Fuad Fia Sri Mumpuni Firda Pratiwi Fuad Fatahillah G. Ayu Arwati, I Ganjar Sidik Gandara Glory Riama Hosianna Gofur, Muhamad Hafidz Anggara Heriansyah, Endang Hermawan Hermawan Hermawan Hermawan Hermawan Thaheer Hermawan, Rino Tri Hery Muhendra Humiras Hardi Purba Irhamni Irhamni Irwanto, Bayu Jamhari Jamhari Jaqin, C Jaqin, Choesnul Jhonny P. Simbolon Joko Sumpono Joko Supono Juliza Hidayati Juniawan, Singgih Kandasamy, J Kartini, Yulfara Kurniawan Agung Susanto Kurniawan, Erwin Ramdhanu Kusuma, Prima Wijaya Lien Herliani Kusumah Mahendra, Dendi Maulani, Bismar Ma’ruf, Amar Meilani, Rahmatika Chasania Meliana, Dita Mirza - MJ, Safruddin Mohamed Mansour Muhamad Atho R Muhammad Fitri, Muhammad Muhammad Wahid Malik Aljabar Muhendra, Hery Nazura, Is Anin Ni Made Ayu Yudawati Parulian, Tunggul Barita Prasetya, Raden Wendrata Prasetyo, Rahmat Dodi Purba, H Hardi Puspasari, Luky Rahmat Dodi Prasetyo Ramli Murgani Rifki Kurniawan Rino Tri Hermawan Rizki, Yulizan Rosalendro Eddy Nugroho Roysen, Uti Rusman Zaenal Abidin Safruddin MJ Sagir Alva Sahirman . Sahirman Sahirman Sahirman Sahirman Saryanto Saryanto Saryanto Saryanto Simbolon, Jhonny P. Sudiyono Sudiyono Sudiyono Sudiyono Suharto H Sumarsono Sudarto Sumpono, Joko Susanto, Kurniawan Agung Syifaul Qulub, Irkham Tunggul Barita Parulian Uly Amrina Victorianus Iva Taufik Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Wiwit Suprihatiningsih Yayan Saputra Yayan Saputra, Yayan Yulian Syahputri Yunita, Ika Zainuddin, Zafitri Zulfa Fitri Ikatrinasari