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Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates Nur Maulida Safitri; Endang Yuli Herawati; Jue Liang Hsu
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.

DETERMINATION OF WATER QUALITY STATUS BASED ON HEAVY METAL CONTENTS IN THE RAINY AND DRY SEASON USING THE STORET INDEX IN PASURUAN SEA WATERS, EAST JAVA Endang Yuli Herawati; Diana Arfiati; Pratama Diffi Samuel; Karina Farkha Dina; Putri Anugerah; Rahmi Valina
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 2 (2021): JFMR VOL 5 NO.2
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.02.3

Waters that are presumably contaminated with heavy metals need to be observed to ensure the level of pollution to perform water restoration. This study was aimed to determine the status of water quality based on heavy metal contents. The survey method was employed in this study, and it was conducted in Pasuruan sea waters in different seasons; September 2019 represented the dry season, and April 2020 represented the rainy season. The water sampling stations were chosen by using purposive sampling in three sampling locations: the sea waters of Kraton, Lekok, and Nguling districts. The heavy metals and water quality parameters were analyzed by using Storet Index. The results indicated that the highest level of heavy metals was found in Lekok waters in September, consisting of Hg (1.22 mg/l), Cd (1.20 mg/l), and Pb (0.55 mg / l). The seawater of Nguling district had the lowest content in April, while Kraton's seawater was moderate. The water quality status based on the Storet Index suggested that the sea waters of Kraton, Lekok, and Nguling districts were classified as moderately polluted with a score of -16 to -24. The highest score was in September in the rainy season with a score of -24 and the score in April in the dry season was -16. It means that the score is increasing but still in the moderately polluted category. The measurement results of temperature, pH, dissolved oxygen, salinity, and current velocity were in optimum results for aquatic organisms in the dry season or the rainy season. Regarding the water quality status in Pasuruan sea waters, it can be concluded that the heavy metal contents exceeded the quality standard set by the government. Therefore, improved management is required to prevent the deterioration of the pollution status in Pasuruan sea waters

Hubungan Kelimpahan Plankton Dengan Pertumbuhan Ikan Bandeng (Chanos chanos Forskal) Di Tambak Tradisional Sidoarjo, Jawa Timur Endang Yuli Herawati; Anik Martinah H.; Qurrota A'yunin; Rully Isfatul H.
EnviroScienteae Vol 15, No 1 (2019): EnviroScienteae Volume 15 Nomor 1, April 2019
Publisher : Universitas Lambung Mangkurat

The surrounding community activities in the area of milkfish ponds include residential and household waste so that it will affect the water source used for milkfish farms (Chanos chanos Forskal). Due to changes in water quality, it will be known how the condition of pond water quality will affect the abundance of phytoplankton and the growth of milkfish (Chanos chanos Forskal). This study aims to determine the relationship of plankton abundance with the growth of milkfish (Chanos chanos Forskal) in traditional ponds. The method used is the descriptive method. The study was conducted in March - May. The results of the regression analysis for plankton abundance found a relationship that affects a specific growth rate of 83% and 17% influenced by other factors. The correlation coefficient (r) obtained is 0.91 so that it can be said that the abundance of plankton influences the specific growth rate classified as strong. Based on the value of b = 0.0156x (positive relationship) means that if plankton abundance rises the rate of growth also increases by 0.015. If the plankton does not exist or 0 then the rate will decrease by 94.

DINAMIKA POPULASI IKAN GABUS (Channa striata) DI RAWA DANAU PANGGANG, KALIMANTAN SELATAN Dini Sofarini; Mohammad Mahmudi; Asus Maizar S. Hertika; Endang Yuli Herawati
EnviroScienteae Vol 14, No 1 (2018): EnviroScienteae Volume 14 Nomor 1, April 2018
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v14i1.4890

The province of South Kalimantan has the largest peat swamp that is named Danau Panggang Swamp with an area of 5,390.7 Ha. This swamp has ecological, economic and potential functions of a large fishery resource. The purposes of this study are to evaluate the exploitation rate of Snake Head Fish (Channa striata) in Danau Panggang Swamp, by population dynamic analysis using FISAT program of Von Bertalanffy method. The results showed that the growth pattern of Snake Head Fish (Channa striata) has the tendency to grow negative allometric with 63.4 cm of long infifer (L¥), growth rate (k) 0.15 per year, theoretical age at the time of the fish is equal to 0 (t0) is -1.48257 years. While the natural mortality rate (M) was 0.43, the mortality rate due to catch (F) of 0.69/year and the total mortality rate (Z) of 1.12/year, and the rate of exploitation value E = 0.62. These results indicated that the exploitation rate of Head Snake Fish (Channa striata) in Danau Panggang swamp has a tendency to overfishing.

KEANEKARAGAMAN SPESIES LAMUN DI KABUPATEN BANGKA SELATAN PROVINSI KEPULAUAN BANGKA BELITUNG Dwi Rosalina; Endang Yuli Herawati; Yenny Risjani; Muhammad Musa
EnviroScienteae Vol 14, No 1 (2018): EnviroScienteae Volume 14 Nomor 1, April 2018
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v14i1.4889

Marine area which is planted by seagrass called as seagrass beds. Seagrass is one of the typical ecosystems in marine waters. Bangka Belitung Province is one of the areas in Indonesia that has seagrass ecosystem. This study aims to determine the diversity, density, and coverage area of seagrass beds in the waters of South Bangka Regency. The research was conducted in Tukak, Tanjung Kerasak, and Tanjung Kemirai. The data is collected using quadratic transect method and also data of physics-chemical parameters such as temperature, salinity, pH, depth, velocity and water substrate. The results of the research showed that 7 species of seagrasses in South Bangka Regency are Cymodocea serrulata, Cymodocea rotundata, Halodule uninervis, Enhalus acoroides, Thalassia hemprichii, Syringodium isoetifolium and Halophila minor. Seagrass in Lempeng waters which has the highest density is Cymodocea serrulata, about 58 ind/m2. The highest frequency of seagrass species in Tukak is Thalassia hemprichii about 1%. Seagrass species of Cymodocea serrulata has the highest cover percentage in Lempeng waters about 29.56%.

Metallothionein levels on the gill and liver of Mystus nigriceps (Valenciennes, 1840) in Welang River, Pasuruan City, East Java Lisma Wardani; Endang Yuli Herawati; Asus Maizar Surya
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

This research was conducted in October 2021 using a survey method. Sampling was carried out three times with a span of every 2 weeks at 3 stations along the lower reaches of the Welang River Pasuruan Regency. Parameters observed included cadmium metal content in fish gills and liver, metallothionein levels in gills, and liver of M. nigriceps. Fish and the relationship between cadmium metal and metallothionein levels in the gills and liver of M. nigriceps in the Welang River. The average yield of cadmium metal content in the gills of M. nigriceps is 5.943-8.103 mg/l. Meanwhile, the average result of heavy metal content in the liver of M. nigriceps is 6.397-9.997 mg/l. Measurement of metallothionein levels showed that the average level of metallothionein in the gills of M. nigriceps was 5.860-8.688 ng/mL. Meanwhile, the average level of metallothionein in the liver of M. nigriceps is 7.110-8.334 ng/mL. And the results of the linear regression test showed that the average content of cadmium in the gills and liver with the average metallothionein levels in the gills and liver of catfish was significantly correlated.

Distribution of heavy metal (Pb, Cd and Hg) concentrations in sediment of Bone River, Gorontalo Miftahul Khair Kadim; Endang Herawati Yuli; Diana Arfiati; Asus Maizar Suryanto Hertika; Faizal Kasim
Depik Vol 11, No 3 (2022): DECEMBER 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

The concentration of three heavy metals Pb, Cd and Hg ware measured in Bone River, Gorontalo sediment. The heavy metal pollution on sediment in Bone River have not been explored longitudinally. The samples were taken in July 2021 at 8 stations. The trend of metals ware observed in sediment as PbCdHg. The level of studied metals Cd and Hg did not exceed the safe limit by ANZECC, OSQG LEL and CCME TEL meanwhile Pb was met the limit by by ANZECC. However, the investigated showed that fluctuations due to differences in the characteristics of each location. Contamination factor (CF) and geoaccumulation index (Igeo) demonstrated that the sediment samples were low contamination and lightly polluted. Contamination by heavy metals in the Bone River implies that the conditions are much frightening for the biota and residents around the river.

STUDI KELAYAKAN LAHAN BUDIDAYA RUMPUT LAUT Eucheuma cottonii di KECAMATAN BLUTO SUMENEP MADURA JAWA TIMUR (Feasibility Study of Eucheuma Cottonii Seaweed Farming in Bluto Subdistric of Sumenep Madura East Java) Abdul Qadir Jailani; Endang Yuli Herawati; Bambang Semedi
Jurnal Manusia dan Lingkungan Vol 22, No 2 (2015): Juli
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18744

ABSTRAKBudidaya rumput laut di Kecamatan Bluto, Kabupaten Sumenep, Jawa Timur, mempunyai kontribusi bagi perekonomian masyarakat pesisir, akan tetapi terdapat kegiatan perikanan seperti penangkapan dan pengolahan hasil laut yang berdampak pada degradasi kualitas air yang berpengaruh langsung kepada hasil produksi rumput laut. Tujuan dari penelitian ini adalah untuk mengetahui lokasi baru pengembangan kegiatan budidaya rumput laut secara berkelanjutan yang terbebas dari kegiatan masyarakat dengan melihat faktor ekologis dan daya dukung perairan di Kecamatan Bluto. Metode yang digunakan mengukur kesesuaian ekologis meliputi suhu, kecerahan, kedalaman, kecepatan arus, kondisi dasar perairan, salinitas, DO, nitrat, ortofosfat, pH, BOD, dan COD, serta estimasi hama dan penyakit rumput laut. Selanjutnya data diskoring untuk menentukan kelas kesesuaian dengan pendekatan SIG. Daya dukung perairan di analisis dengan menghitung 60% dari jumlah kawasan yang sesuai. Hasil penelitian menunjukkan bahwa perairan Bluto memiliki potensi lahan pengembangan rumput laut berdasarkan kelas S1 (sangat sesuai) dan S2 (sesuai bersyarat) seluas 770,19 ha, dan mampu menampung maksimal 42.788 unit rakit rumput laut. Hama dan penyakit yang teridentifikasi di perairan Bluto adalah ikan baronang (Siganus. sp), ice-ice, lumut (Chaetomorpha crassa), dan teritip (Chthamalus stellatus). ABSTRACTSeaweed farming in the subdistrict Bluto, District Sumenep, East Java, has significant contribution in the economy of coastal communities, but there are fishing activities like catching up and marine product proccessing that have negative impact to the degradation of water quality and then directly affect the production of seaweed. The purpose of this study is find new location of the development of seaweed farming activities in a sustainable manner that is free from of community activities with a view of ecological factors and carrying capacity of the waters in the analies of the subdistrict Bluto. The method was used to analyze its ecological suitability includes temperature, transparency, depth, current velocity, bottom water condition, salinity, DO, Nitrite, ortophosphate, pH, BOD, and COD, also evaluation of the predators and diseases seaweed. Then, the data is scored to determine its suitability class with SIG approach. Environmental carrying capacity is analyzed by determining 60% of suitable sites. The result showed that Bluto territorial waters was potential for seaweed farming development as it was in Class S1(very suitable) and S2 (suitable with conditions) about 770.19 ha and able to contain maximum 42,788 units of seaweed floated bamboos. Predators and diseases identified in Bluto waters are rabbitfishes (Siganus. sp), ice-ice disease, green algae (Chaetomorpha crassa), and Poli’s stellate barnacle (Chthamalus stellatus).

Metallothionein levels on the gill and liver of Mystus nigriceps (Valenciennes, 1840) in Welang River, Pasuruan City, East Java Lisma Wardani; Endang Yuli Herawati; Asus Maizar Surya
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.24748

This research was conducted in October 2021 using a survey method. Sampling was carried out three times with a span of every 2 weeks at 3 stations along the lower reaches of the Welang River Pasuruan Regency. Parameters observed included cadmium metal content in fish gills and liver, metallothionein levels in gills, and liver of M. nigriceps. Fish and the relationship between cadmium metal and metallothionein levels in the gills and liver of M. nigriceps in the Welang River. The average yield of cadmium metal content in the gills of M. nigriceps is 5.943-8.103 mg/l. Meanwhile, the average result of heavy metal content in the liver of M. nigriceps is 6.397-9.997 mg/l. Measurement of metallothionein levels showed that the average level of metallothionein in the gills of M. nigriceps was 5.860-8.688 ng/mL. Meanwhile, the average level of metallothionein in the liver of M. nigriceps is 7.110-8.334 ng/mL. And the results of the linear regression test showed that the average content of cadmium in the gills and liver with the average metallothionein levels in the gills and liver of catfish was significantly correlated.

Distribution of heavy metal (Pb, Cd and Hg) concentrations in sediment of Bone River, Gorontalo Miftahul Khair Kadim; Endang Herawati Yuli; Diana Arfiati; Asus Maizar Suryanto Hertika; Faizal Kasim
Depik Vol 11, No 3 (2022): DECEMBER 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.3.27775

The concentration of three heavy metals Pb, Cd and Hg ware measured in Bone River, Gorontalo sediment. The heavy metal pollution on sediment in Bone River have not been explored longitudinally. The samples were taken in July 2021 at 8 stations. The trend of metals ware observed in sediment as PbCdHg. The level of studied metals Cd and Hg did not exceed the safe limit by ANZECC, OSQG LEL and CCME TEL meanwhile Pb was met the limit by by ANZECC. However, the investigated showed that fluctuations due to differences in the characteristics of each location. Contamination factor (CF) and geoaccumulation index (Igeo) demonstrated that the sediment samples were low contamination and lightly polluted. Contamination by heavy metals in the Bone River implies that the conditions are much frightening for the biota and residents around the river.

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