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The Analysis of Hepatopancreas Histologycal Damage in Neocallichirus karumba (Poore and Griffin) Shrimp Caused by Heavy Metal Pb Exposure in Madura Strait Kristiani, Maria; Herawati, Endang Yuli; Yanuhar, Uun
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1093.074 KB) | DOI: 10.21776/ub.jels.2016.006.01.06

Abstract

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.
White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District Usman, Ali; Andayani, Sri; Herawati, Endang Yuli
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1034.385 KB) | DOI: 10.21776/ub.jels.2018.008.03.07

Abstract

Vanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms (L. vannamei) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%.Keywords: PCR, Prevalence, Shrimp, Vannamei, WSSV.
Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Tega, Yatris Rambu; Herawati, Endang Yuli; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1146.597 KB) | DOI: 10.21776/ub.jels.2019.009.02.13

Abstract

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.
Metallothionein levels on the gill and liver of Mystus nigriceps (Valenciennes, 1840) in Welang River, Pasuruan City, East Java Wardani, Lisma; Herawati, Endang Yuli; Surya, Asus Maizar
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.24748

Abstract

This research was conducted in October 2021 using a survey method. Sampling was carried out three times with a span of every 2 weeks at 3 stations along the lower reaches of the Welang River Pasuruan Regency. Parameters observed included cadmium metal content in fish gills and liver, metallothionein levels in gills, and liver of M. nigriceps. Fish and the relationship between cadmium metal and metallothionein levels in the gills and liver of M. nigriceps in the Welang River. The average yield of cadmium metal content in the gills of M. nigriceps is 5.943-8.103 mg/l. Meanwhile, the average result of heavy metal content in the liver of M. nigriceps is 6.397-9.997 mg/l. Measurement of metallothionein levels showed that the average level of metallothionein in the gills of M. nigriceps was 5.860-8.688 ng/mL. Meanwhile, the average level of metallothionein in the liver of M. nigriceps is 7.110-8.334 ng/mL. And the results of the linear regression test showed that the average content of cadmium in the gills and liver with the average metallothionein levels in the gills and liver of catfish was significantly correlated.
Co-Authors A'yunin, Qurrota A.P.W Marhendra Abdul Aziz Amin Abdul Qadir Jailani Abdul Qadir Jailani, Abdul Qadir Abu Bakar Sambah Abu Bakar Sambah, Abu Bakar Aida Sartimbul Ainun Ramadhani Tri Wahyuni Ali Usman, Ali Aminin, Aminin Aminuddin Firdaus Anam, C Andi Kurniawan Anjasmara, Aang Setyawan Anugerah, Putri Anwari, Zaki Asus Maizar Surya Asus Maizar Suryanto H Ating Yuniarti Azis Husen, Azis Azizah, Nur Aini Bambang Semedi C Anam Chandika Lestariaji Citra Satrya Utama Dewi Daduk Setyohadi Dewa Gede Raka Wiadnya Dewa Gede Raka Wiadnya Dewi, Citra Satrya Utama Diana Arfiati Dina, Karina Farkha Dini Sofarini Dwi Hindarti, Dwi Dwi Rosalina Eddy Suprayitno Faizal Kasim Fani Fariedah Firdaus, Aminuddin Happy Nursyam Hariati, Anik Martinah Jue Liang Hsu Kiki Riski Arisandy Kristiani, Maria Kusriani Kusriani Kusriani Kusriani Kusuma, Ni Putu Dian Lisma Wardani Marhendra, A.P.W Miftahul Khair Kadim Mohammad Mahmudi Moore, Abigail Mary Muammar Kadhafi Muhammad Musa Muhklas Shah Winarno Nainggolan, Dicky Willy Nanik Retno Buwono Ni Putu Dian Kusuma Nur Maulida Safitri Nur Syahid, Nur Pratama Diffi Samuel Priska Ristianadewi Puspitasari, Asthervina Widyastami Putri Anugerah Rahmi Valina Rahmi Valina Rully Isfatul H. Ruly Isfatul Khasanah Ruly Isfatul Khasanah Saddam Langkung Djaduk Samliok Ndobe Samuel, Pratama Diffi Soemarno _, Soemarno Sri Andayani Sukandar Sukandar Surya, Asus Maizar Umi Zakiyah, Umi Valina, Rahmi Wardani, Lisma Winarno, Muhklas Shah Yanuhar, Uun Yatris Rambu Tega Yenny Risjani Yuni Kilawati