Claim Missing Document
Check
Articles

Found 35 Documents
Search

Do Biodegradable Microplastics Threaten Chlorella spp.? First Study in Indonesia Using Leachate Exposure Azizah, Nur Aini; Hindarti, Dwi; Herawati, Endang Yuli; Nainggolan, Dicky Willy; Sartimbul, Aida
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.08

Abstract

Biodegradable plastics are increasingly being promoted as environmentally friendly alternatives to conventional plastics. However, under aquatic conditions, their degradation may remain incomplete, leading to the formation of microplastics and leachates containing potentially toxic compounds. These leachates can threaten aquatic organisms, particularly microalgae, which act as primary producers and form the base of marine food web. Despite these concerns, limited research has examined the sublethal toxicity of biodegradable microplastic leachates on microalgae. This study aimed to evaluate the toxic effects of leachates derived from biodegradable polypropylene (PP) and high-density polyethylene (HDPE) plastics on the growth of the green microalgae Chlorella spp. A 96-hour static toxicity test was conducted under laboratory conditions using a completely randomized design with five concentration levels (0, 0.1, 1.0, 10, and 100 mg/L). The algae were cultured in EDTA-free Walne medium with an initial cell density of 104 cells/mL. Cell density was measured at the end of exposure to determine growth inhibition and calculate the 50% inhibitory concentration (IC50). The results showed a clear concentration-dependent response, with PP leachate exhibiting significantly higher toxicity (IC50 = 0.48 mg/L) compared to HDPE leachate (IC50 = 682.49 mg/L). These findings suggest that the chemical composition and leachability of additives differ among plastic types, influencing their environmental impact. This study highlights the potential ecological risks of biodegradable plastics and emphasizes the need for stricter regulation of additives and comprehensive ecotoxicological assessments.
Growth Pattern and Morphometric Characteristics of Keting Mystus gulio (Hamilton, 1822) in Downstream of Gembong and Rejoso River, Pasuruan, Indonesia Chandika Lestariaji; Endang Yuli Herawati; Diana Arfiati; Saddam Langkung Djaduk; Priska Ristianadewi; Aang Setyawan Anjasmara; Rahmi Valina
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.10175

Abstract

Keting fish is a type of fish from freshwater which is usually used as a consumption fish or ornamental fish. There is a type of kein, namely from the species Mystus gulio (Hamilton, 1922). Mystus gulio ecosystem is disturbed due to land-use change activities in the river which causes the disturbance of the habitat of Mystus gulio. The purpose of this study is to analyze the long-weight relationship, growth pattern, and condition factors of Mystus gulio in downstream Gembong and  Rejoso River. The method used in this study is to sample Mystus gulio from 3 stations on each river based on land use criteria. The research was carried out for 3 months, namely August, September and October 2023. The results of this study show that Mystus gulio caught in these two rivers all have a negative allometric growth pattern (b<3). The average value of the condition factor in these two rivers is 1 which indicates that the condition of Mystus gulio is good. The average growth of Mystus gulio caught in Gembong is relatively larger than Rejoso. The waters quality results of Gembong and Rejoso are still in optimal condition, except for Total Suspended Solid and Lead (Pb).
The Analysis of Hepatopancreas Histologycal Damage in Neocallichirus karumba (Poore and Griffin) Shrimp Caused by Heavy Metal Pb Exposure in Madura Strait Kristiani, Maria; Herawati, Endang Yuli; Yanuhar, Uun
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1093.074 KB) | DOI: 10.21776/ub.jels.2016.006.01.06

Abstract

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.
White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District Usman, Ali; Andayani, Sri; Herawati, Endang Yuli
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1034.385 KB) | DOI: 10.21776/ub.jels.2018.008.03.07

Abstract

Vanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms (L. vannamei) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%.Keywords: PCR, Prevalence, Shrimp, Vannamei, WSSV.
Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Tega, Yatris Rambu; Herawati, Endang Yuli; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1146.597 KB) | DOI: 10.21776/ub.jels.2019.009.02.13

Abstract

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.