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All Journal International Journal of Evaluation and Research in Education (IJERE) Jurnal Ilmu Pertanian Indonesia Jurnal Pendidikan Biologi Undiksha Microbiology Indonesia Abdimas Biology, Medicine, & Natural Product Chemistry JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika Jurnal Biologi Tropis Bioedukasi: Jurnal Pendidikan Biologi Jambura Edu Biosfer Journal Ideas: Jurnal Pendidikan, Sosial dan Budaya JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Journal of Tropical Ethnobiology Bantara Journal of Animal Science Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Didaktika: Jurnal Kependidikan Research Review: Jurnal Ilmiah Multidisiplin Biodik: Jurnal Ilmiah Pendidikan Biologi HUMANIORASAINS Jurnal Cendekia Ilmiah Journal of Research in Instructional
Abubakar Sidik Katili
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Phosphate-Solubilizing and Indole Acetic Acid-Producing Actinomycetes from the Plant Rhizosphere in the Hungayono Karst, Gorontalo Yusriyah Yusriyah; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.907-917

Abstract

Actinomycetes are soil microorganisms that play a crucial role in nutrient cycles and interactions, particularly in nutrient-limited environments such as karst ecosystems. This study aimed to evaluate the preliminary functional potential of actinomycetes isolated from the plant rhizosphere in the Hungayono karst ecosystem, Gorontalo, as phosphate solubilizers and producers of indole acetic acid (IAA). The study employed a quantitative descriptive approach with soil sampling at a depth of 10–15 cm using purposive sampling. Isolation was performed using the spread plate method on Starch Casein Agar medium, followed by qualitative and quantitative testing of phosphate solubilization and IAA production capabilities. Identification of selected isolates was conducted through 16S rRNA gene-based phylogenetic analysis. The results showed that 11 actinomycete isolates were obtained from 4 host plants, of which two isolates exhibited low-index qualitative phosphate solubilization activity. However, quantitative results indicated that some isolates that did not form clear zones still produced measurable amounts of soluble phosphate, suggesting differences in method sensitivity. The highest phosphate-solubilizing activity was exhibited by isolate RzPO-09. In addition, four isolates were capable of producing IAA at varying levels, with the highest production observed in isolate RzPH-07 at 0.896 ppm. Phylogenetic analysis showed that isolate RzPO-09 (PZ458677) is closely related to the genus Pseudonocardia, while RzPH-07 (PZ458434) is closely related to the genus Streptomyces. Overall, the isolates obtained show initial functional potential; however, further validation is required through optimization of culture conditions, genetic characterization, plant growth tests, and testing both in greenhouses and in the field to ensure their practical feasibility.
Penerapan Model Pembelajaran Kooperatif Numbered Head Together (NHT) terhadap Hasil Belajar Siswa Pada Mata Pelajaran Biologi Materi Ekosistem Di Sma Negeri 1 Limboto Barat Yudit O. R. Rumbekwan; Lilan Dama; Abubakar Sidik Katili
Research Review: Jurnal Ilmiah Multidisiplin Vol. 2 No. 2 (2023): Research Review: Jurnal Ilmiah Multidisiplin (Agustus 2023 - Januari 2024)
Publisher : Transbahasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54923/researchreview.v2i2.39

Abstract

This research is an experimental research that aims to determine the effect of the Numbered Head Together (NHT) learning model on biology learning outcomes of SMA N 1 Limboto Barat, Gorontalo on ecosystem material with the Nonequivalent Control Group Design research design. The population in this study consisted of three classes, namely class X MIPA 1 with a total of 20 students and X MIPA 19 with a total of 30 students as an experimental class and class X IPS 1 with a total of 14 students as a control class. The research variable consists of the independent variable, namely the use of Number Head Together (NHT), while the dependent variable is student learning outcomes. Data collection was carried out through pre-test and post-test. Based on the results of the study, it was shown that the average post-test learning outcomes for students in class X MIPA 1 and X MIPA 2 as an experimental class taught using the Numbered Heads Together (NHT) model, obtained an average result of 74.92, while the results the average learning obtained by students of class X IPS 1 as a control class taught using the conventional model is 51.12. Furthermore, the control class experienced an increase in student graduation by 21%, while the experimental class experienced an increase in student graduation by 64%, which means that the value of student learning outcomes increased more than threefold with the treatment of using the Numbered Heads Together (NHT) model, especially in the experimental class.
AKTIVITAS ANTIBAKTERI ACTINOMYCETES DARI AKAR TANAMAN DI EKOSISTEM KARST GORONTALO, INDONESIA Alfandi Kibu; Yuliana Retnowati; Novri Youla Kandowangko; Wirnangsi Din Uno; Abubakar Sidik Katili; Nurdin
Jurnal Humaniora & Sosial Sains Vol 2 No 1 (2025)
Publisher : Pojok Publisher

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Abstract

Several pathogenic bacteria such as Staphylococcus aureus, Escherichia, Pseudomonas aeruginosa and other pathogenic bacteria have developed resistance to many marketed antibiotics. Staphylococcus aureus is already resistant to several types of antibiotics such as penicillin, erythromycin, cefoxitin, levofloxacin, and clindamycin, Escherichia is resistant to several antibiotics such as Penicillin G, Cefotaxime, Tetracycline, Ampicillin, and Amoxicillin, Pseudomonas aeruginosa has been resistant to antibiotics that target ribosomes, such as tobramycin and tigecycline. The problem of resistance of several pathogenic bacteria can be overcome by finding new metabolite compounds from Actinomycetes microorganisms that have antibiotic potential by exploring extreme areas, one of which is in the karst area, especially in plant roots. Root sampling was carried out by taking samples + 20 cm on each type of plant found. Isolation of Actinomycetes bacteria was carried out using the plate method. Antibacterial activity testing was carried out using the line method. The morphological form of the spores was seen using a SEM (Scanning Electron Microscope). The results of the study found 2 isolates of isolated plant roots containing Actinomycetes bacteria. 2 isolates showed morphological characteristics of mycelium, oval and round in shape and had various colors. The results of the antibacterial activity test showed that each isolate could inhibit several types of pathogenic bacteria, seen from the clear zone with different diameters and the results of SEM (Scanning Electron Microscopy) showed that 2 isolates had the same character and shape of spores as Actinomycetes, namely the genus Streptomyces.
STRUKTUR VEGETASI MANGROVE DI DESA POPALO KABUPATEN GORANTALO UTARA Nurlena Duhe; Marini Susanti Hamidun; Abubakar Sidik Katili; Febriyanti; 5Ilyas H. Husain; Waode Faridawaty
Jurnal Humaniora & Sosial Sains Vol 2 No 1 (2025)
Publisher : Pojok Publisher

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Abstract

This study focuses on observing the structure of mangrove vegetation in Popalo Village, North Gorontalo Regency. Scientists found that the condition of mangrove forests in the area is not uniform, with some areas still well maintained, especially near rivers and beaches, while other areas, especially near settlements, have been damaged due to land conversion into ponds. This type of research is descriptive quantitative using a survey method. The most dominant type of mangrove found is Rhizophora. In addition, this study also shows that environmental factors such as temperature, air acidity, and salt levels greatly affect the growth and development of mangroves. Overall, the results of this study highlight the importance of better conservation and management efforts for mangrove forests in Popalo Village to maintain the sustainability of coastal ecosystems.
POTENSI ACTINOMYCETES DARI RHIZOSFER TUMBUHAN DI EKOSISTEM KARST GORONTALO SEBAGAI ANTICANDIDA Riskanarti K. Lihaawa; Yuliana Retnowati; Abubakar Sidik Katili
Jurnal Humaniora & Sosial Sains Vol 2 No 1 (2025)
Publisher : Pojok Publisher

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Abstract

Karst ecosystem is one of the extreme marginal areas or types of soil that has low soil fertility. Soil in the karst ecosystem has a relatively high calcium (Ca) content which can affect the availability of phosphorus (P). However, there is microbial activity that can provide nutrient needs for plants to stay alive, one of which is Actinomycetes. This research method uses a quantitative research method, rhizosphere soil samples were taken at three locations, namely Bangga hill, Panipi hill around Lake Limboto, and Olohuta hill with a purposive sampling method. The results of the study showed that there were 7 isolates of Actinomycetes found associated with 5 types of plants in the karst ecosystem. Actinomycetes isolates showed almost uniform morphological, colony and cell characteristics dominated by white. The results of the anticandida activity test showed that there were 2 isolates (KSLl and KSIc) that inhibited the growth of Candida albicans, KSLl with an inhibition zone diameter of 10.5 mm and KSIc with an inhibition zone diameter of 17.5 mm. Molecular identification based on 16S RRNA gene sequences showed that the KSLl isolate was closely related to Streptomyces aegyptia with a similarity index of 99.64% and the KSIc isolate was closely related to Istreptomyces sp with a similarity index of 99.57%.
Analisis Kemampuan Siswa Kelas XI alam Menjawab Soal Berbasis HOTS Pada Materi Sel Siti Djuhriyanti Karim; Ani M. Hasan; Nur Mustaqimah; Abubakar Sidik Katili; Magfirahtul Jannah
Jurnal Pendidikan Biologi Undiksha Vol. 13 No. 2 (2026): In Progress
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v13i2.115583

Abstract

Salah satu keterampilan yang sangat diperlukan pada abada 21 adalah kemampuan berpikir tingkat tinggi atau HOTS (Higher Order Thinking Skills), yang mencakup kemampuan untuk menganalisis, mengevaluasi, dan menciptakan. Penelitian ini menganalisis kemampuan siswa SMA Negeri 2 Limboto dalam menyelesaikan soal berbasis HOTS pada materi sel. Penelitian ini menggunakan kuantitatif deskriptif dengan melibatkan 31 siswa kelas XI sebagai subjek penelitian. Instrumen yang digunakan berupa tes HOTS yang terdiri dari enam soal berbentuk esai, yang masing-masing mengukur kemampuan siswa pada level kognitif C4 (menganalisis), C5 (mengevaluasi), dan C6 (mencipta). Hasil penelitian menunjukkan bahwa mayoritas siswa memperoleh nilai yang tergolong rendah, dengan rata-rata skor 35.87 dan standar deviasi 18.39. Daya serap siswa pada level C4 tercatat 41,29%, C5 35,69%, dan C6 23,23%, yang menandakan kesulitan signifikan pada aspek evaluasi dan penciptaan. Temuan ini menunjukkan bahwa meskipun ada sebagian siswa yang menunjukkan kemampuan yang lebih baik, upaya pengajaran yang lebih terstruktur masih diperlukan untuk meningkatkan keterampilan berpikir tingkat tinggi siswa, khususnya dalam evaluasi dan Mencipta.
Antagonistic Ability and Fungicide Tolerance of Actinomycetes from the Rhizosphere of Maize (Zea mays L.) Plants in Gorontalo Danial Mohamad; Yuliana Retnowati; Novri Youla Kandowangko; Abubakar Sidik Katili; Wirnangsi Din Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1193-1201

Abstract

Actinomycetes are a group of microbes that live in the rhizosphere of plants and play an important role in soil microbial dynamics. This study aims to describe the antagonistic ability against pathogenic molds and tolerance to fungicides in actinomycetes from the rhizosphere of maize plants in Gorontalo. This research is descriptive uses a quantitative approach and employs a purposive sampling technique. Rhizosphere soil samples were collected from maize fields in the hilly area of Bontula Village, Asparaga District, Gorontalo Regency. The results showed the presence of 7 actinomycetes isolates with different morphological characteristics, namely RFZm-PG, RFZm-PLG, RFZm-PW, RFZm-PB, RFZm-PR, RFZm-PY, and RFZm-PO. Antagonistic tests showed that four isolates (RFZm-PB, RFZm-PG, RFZm-PLG, RFZm-PW) inhibited the growth of Fusarium oxysporum and Neocosmospora solani with an inhibition percentages of 66.38%-89%. The fungicide tolerance test showed that the seven isolates were tolerant to thiram, dimethomorph and benomyl, with the highest inhibitory effect by thiram. Isolates RFZm-PG and RFZm-PLG had the highest tolerance up to 500 ppm, while other isolates were tolerant only up to 400 ppm. Molecular analysis showed that RFZm-PG belongs to the genus Streptomyces (100% similarity with Streptomyces enissocaesilis), while RFZm-PLG belongs to Nocardiopsis (100% similarity with Nocardiopsis synnemataformans).
Distribution and Abundance of Actinomycetes in the Rhizosphere Soil of The Hungayono Karst Ecosystem, Gorontalo Apriliyanti Mohulaingo; Yuliana Retnowati; Abubakar Sidik Katili; Ani M. Hasan; Novri Youla Kandowangko
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1363-1371

Abstract

Karst ecosystems have unique environmental conditions that can influence the presence of soil microorganisms, including Actinomycetes in the rhizosphere. This study aimed to determine the distribution and abundance of Actinomycetes in the rhizosphere soil of plants in the Hungayono karst ecosystem, Gorontalo. This study employed a quantitative descriptive method with an exploratory approach. Rhizosphere soil samples were collected from several plant species at three observation points: the lower, middle, and upper parts of the karst area. Actinomycetes were isolated using Starch Casein Agar, while their abundance was determined using the Total Plate Count method and expressed in CFU/g of soil. The results showed that actinomycetes were found at all observation points, with abundances ranging from 2 × 103 to 3 × 105 CFU/g. The highest abundances were found in the rhizosphere of Calamus spp., Anthocephalus macrophyllus, and Ficus microcarpa, while the lowest abundances were found in the rhizosphere of Alocasia macrorrhizos. The Actinomycetes isolates obtained exhibited morphological variation based on mycelium color and colony shape. These findings indicate that the distribution and abundance of Actinomycetes in the rhizosphere soil of the Hungayono karst ecosystem vary among plant species and observation sites. The plant rhizosphere in this karst ecosystem functions as a microhabitat that supports the presence of Actinomycetes and is relevant to studies of karst soil microbiology.
Effectiveness of the PjBL-STEAM-IC model in improving students’ environmental awareness in coastal schools Srilesnawati Lalusu; Elya Nusantari; Frida Maryati Yusuf; Abubakar Sidik Katili; Hartono Mamu; Muhammad Rifai Katili; Masra Latjompoh
Journal of Research in Instructional Vol. 6 No. 1 (2026): Journal of Research in Instructional
Publisher : Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jri.v6i1.6

Abstract

Coastal ecosystem degradation in Pagimana District, combined with science instruction that remains largely theoretical, underscores to the need for a learning model that can foster students’ environmental awareness more comprehensively. This study examined the effectiveness of the PjBL-STEAM-IC model in improving students’ environmental awareness in coastal schools across the four KABS dimensions: knowledge, attitude, behavior, and skills. The study was part of a Research and Development project and specifically reports the field-testing phase, which included limited trials, wider trials, and operational implementation. Participants were 150 seventh-grade students from four coastal junior high schools in Pagimana District. Data were collected using an environmental awareness questionnaire and analyzed using descriptive statistics, N-gain, the Wilcoxon Signed-Rank Test, and the Kruskal-Wallis Test. The results showed that the PjBL-STEAM-IC model effectively improved students’ environmental awareness across all four dimensions. The mean score increased from 67.41 before implementation to 86.81 after implementation, with N-gain values ranging from 0.62 to 0.64, and all dimensions showed significant differences (p < 0.001). These findings suggest that the PjBL-STEAM-IC model is a promising contextual science learning alternative for strengthening environmental awareness in coastal schools.
Insect Community Structure in Corn Plantations and the B.J. Habibie Forest Park (Tahura) Nazwa H. Djafar; Chairunnisah J. Lamangantjo; Mustamin Ibrahim; Ilyas H. Husain; Abubakar Sidik Katili
Bantara Journal of Animal Science Vol. 8 No. 1 (2026): BJAS
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v8i1.8524

Abstract

The conversion of forest land into cornfields in the Bontula Village Buffer Forest, Tahura BJ Habibie, has caused changes in vegetation and environmental conditions that have impacted insect diversity. This study aims to analyze the structure of insect communities in the buffer forest habitat and the corn fields in Bontula Village. The study employed a quantitative descriptive method with a comparative approach. Sampling was conducted using purposive sampling with a combination of pitfall traps and light traps. The collected insects were identified to the species level and then analyzed using the Shannon-Wiener diversity index (H'), abundance (Di), evenness (E), species richness (R), and dominance (D). The results showed that 1,460 insect individuals belonging to 24 species and 21 families were found. The diversity index value for the buffer forest (H' = 1.376) was higher than that for the corn plantation (H' = 1.091), but both fell into the moderate category. The species with the highest abundance was Novomessor cockerelli, with a Di value of 0.415% in the buffer forest and 0.588% in the corn plantation. The evenness index was moderate, with a value of E = 0.508 in the buffer forest and E = 0.439 in the corn plantation. The species richness indices were R = 2.031 and R = 1.784, respectively, while the dominance indices were D = 0.302 and D = 0.441. In general, the insect community structure in both habitats was relatively stable, with a fairly good ecological balance.
Co-Authors 5Ilyas H. Husain Abdul Haris Odja Ahmad Zainuri Alfandi Kibu Almira Rayyah Shadriah Fahru Amelia Endang Puluhulawa Ani M. Hasan Anisa Nurrismawati Apriliyanti Mohulaingo Bambang Mamangkay Budy Santoso Chairunnisah J Lamangantjo Citra Leonita Matalauni Danial Mohamad Dewi Wahyuni K. Baderan Diana Vivanti Sigit Djuna Lamondo Elya Nusantari Fathur Rahman M. Ali Fatma waty Ishak Febriyanti Frida Maryati Yusuf Hajra Paune Hartono D Mamu Hartono Mamu Herinda Mardin Ilyas H. Husalin Ilyas Husain Indra Domili Ismiyati Uno Juinten Hogi Jusna Ahmad Ledy Mutmainah Y Syahril Lilan Dama Magfirahtul Jannah Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Moh Rizal N. Matau Moh. Fahri Haruna Moh. Gifari Hi Sahada Mustamim Ibrahim Mustamin Ibrahim Nazifah R Adam Nazwa H. Djafar Novi Safitri Lamangida Novian Swasono Hadi Novri Youla Kandowangko Nur Mustaqimah Nurdin Nurfadila Potutu Nurlena Duhe Pangalo, Paulus Puji Prastowo Rahmatia Karuana Ramli Utina Ratna Komala Rauf A. Hatu Regina Valentine Aydalina Riskanarti K. Lihaawa Rizhal Hendi Ristanto Silpian Mohamad Silvana Ariska Ridwan Sintia N. Hasani Siti Djuhriyanti Karim Siti Marwah S Sri Yustika Candri Pakaya Srilesnawati Lalusu Syam S. Kumaji Tiska Rasyid Vivien Novarina Kasim Waode Faridawaty Wirnangsi D Uno Wirnangsih D. Uno Yowan Tamu Yudit O. R. Rumbekwan Yuliana Retnowati Yusriyah Yusriyah Zohra Waty Hiola Zohrawaty Hiola Zuliyanto Zakaria