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All Journal International Journal of Evaluation and Research in Education (IJERE) Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia Abdimas Biology, Medicine, & Natural Product Chemistry JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika Jurnal Biologi Tropis Jambura Edu Biosfer Journal Ideas: Jurnal Pendidikan, Sosial dan Budaya JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Journal of Tropical Ethnobiology Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Didaktika: Jurnal Kependidikan Research Review: Jurnal Ilmiah Multidisiplin Biodik: Jurnal Ilmiah Pendidikan Biologi Jurnal Cendekia Ilmiah
Abubakar Sidik Katili
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Phosphate-Solubilizing and Indole Acetic Acid-Producing Actinomycetes from the Plant Rhizosphere in the Hungayono Karst, Gorontalo Yusriyah Yusriyah; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.907-917

Abstract

Actinomycetes are soil microorganisms that play a crucial role in nutrient cycles and interactions, particularly in nutrient-limited environments such as karst ecosystems. This study aimed to evaluate the preliminary functional potential of actinomycetes isolated from the plant rhizosphere in the Hungayono karst ecosystem, Gorontalo, as phosphate solubilizers and producers of indole acetic acid (IAA). The study employed a quantitative descriptive approach with soil sampling at a depth of 10–15 cm using purposive sampling. Isolation was performed using the spread plate method on Starch Casein Agar medium, followed by qualitative and quantitative testing of phosphate solubilization and IAA production capabilities. Identification of selected isolates was conducted through 16S rRNA gene-based phylogenetic analysis. The results showed that 11 actinomycete isolates were obtained from 4 host plants, of which two isolates exhibited low-index qualitative phosphate solubilization activity. However, quantitative results indicated that some isolates that did not form clear zones still produced measurable amounts of soluble phosphate, suggesting differences in method sensitivity. The highest phosphate-solubilizing activity was exhibited by isolate RzPO-09. In addition, four isolates were capable of producing IAA at varying levels, with the highest production observed in isolate RzPH-07 at 0.896 ppm. Phylogenetic analysis showed that isolate RzPO-09 (PZ458677) is closely related to the genus Pseudonocardia, while RzPH-07 (PZ458434) is closely related to the genus Streptomyces. Overall, the isolates obtained show initial functional potential; however, further validation is required through optimization of culture conditions, genetic characterization, plant growth tests, and testing both in greenhouses and in the field to ensure their practical feasibility.
Penerapan Model Pembelajaran Kooperatif Numbered Head Together (NHT) terhadap Hasil Belajar Siswa Pada Mata Pelajaran Biologi Materi Ekosistem Di Sma Negeri 1 Limboto Barat Yudit O. R. Rumbekwan; Lilan Dama; Abubakar Sidik Katili
Research Review: Jurnal Ilmiah Multidisiplin Vol. 2 No. 2 (2023): Research Review: Jurnal Ilmiah Multidisiplin (Agustus 2023 - Januari 2024)
Publisher : Transbahasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54923/researchreview.v2i2.39

Abstract

This research is an experimental research that aims to determine the effect of the Numbered Head Together (NHT) learning model on biology learning outcomes of SMA N 1 Limboto Barat, Gorontalo on ecosystem material with the Nonequivalent Control Group Design research design. The population in this study consisted of three classes, namely class X MIPA 1 with a total of 20 students and X MIPA 19 with a total of 30 students as an experimental class and class X IPS 1 with a total of 14 students as a control class. The research variable consists of the independent variable, namely the use of Number Head Together (NHT), while the dependent variable is student learning outcomes. Data collection was carried out through pre-test and post-test. Based on the results of the study, it was shown that the average post-test learning outcomes for students in class X MIPA 1 and X MIPA 2 as an experimental class taught using the Numbered Heads Together (NHT) model, obtained an average result of 74.92, while the results the average learning obtained by students of class X IPS 1 as a control class taught using the conventional model is 51.12. Furthermore, the control class experienced an increase in student graduation by 21%, while the experimental class experienced an increase in student graduation by 64%, which means that the value of student learning outcomes increased more than threefold with the treatment of using the Numbered Heads Together (NHT) model, especially in the experimental class.
Co-Authors Abdul Haris Odja Ahmad Zainuri Almira Rayyah Shadriah Fahru Amelia Endang Puluhulawa Ani M. Hasan Anisa Nurrismawati Bambang Mamangkay Budy Santoso Chairunnisah J Lamangantjo Citra Leonita Matalauni Dewi Wahyuni K. Baderan Diana Vivanti Sigit Djuna Lamondo Elya Nusantari Fathur Rahman M. Ali Fatma waty Ishak Frida Maryati Yusuf Hajra Paune Hartono D Mamu Hartono Mamu Herinda Mardin Ilyas H. Husalin Ilyas Husain Indra Domili Ismiyati Uno Juinten Hogi Jusna Ahmad Ledy Mutmainah Y Syahril Lilan Dama Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Moh. Fahri Haruna Moh. Gifari Hi Sahada Mustamin Ibrahim Nazifah R Adam Novi Safitri Lamangida Novian Swasono Hadi Novri Youla Kandowangko Nurfadila Potutu Pangalo, Paulus Puji Prastowo Rahmatia Karuana Ramli Utina Ratna Komala Rauf A. Hatu Regina Valentine Aydalina Rizhal Hendi Ristanto Silpian Mohamad Silvana Ariska Ridwan Sintia N. Hasani Siti Marwah S Sri Yustika Candri Pakaya Syam S. Kumaji Vivien Novarina Kasim Wirnangsi D Uno Wirnangsih D. Uno Yowan Tamu Yudit O. R. Rumbekwan Yuliana Retnowati Yusriyah Yusriyah Zohra Waty Hiola Zohrawaty Hiola Zuliyanto Zakaria