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Efek Imunostimulator Kubis (Brassica Oleracea Var. Capitata Alba) Terhadap Titer Imunoglobulin G (Ig G) Pada Kelinci Yang Diinduksi Dengan Sel Darah Merah Domba Inayati, Nurul; Fihiruddin, Fihiruddin; Getas, I Wayan
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.196

Abstract

Kubis (Brassica aloracae var.capitata alba) adalah salah satu sayuran yang berpotensi sebagai imunostimulator. Kubis banyak megandung nutrien yang mempunyai nutrisi tinggi seperti vitamin, mineral, dietary fiber, glukosinolates, polyphenol dan phenolic acid. Disamping itu juga kubis mengandung peptida yang berperan sebagai imunoglobulin production stimulating factor (IPSF) sehingga dapat berfungsi sebagai imunostimulator yang akan dapat meningkatkan sistem imun tubuh dengan cara merangsang peningkatan sistem fagositik dan produksi interleukin 5. Penelitian ini merupakan penelitian ekprimental dengan desain statistic group comparation yang bertujuan mengetahui pengaruh pemberian kubis (Brassica oleracea capitata alba) untuk meningkatkan pembentukan immunoglobulin G (IgG) pada kelinci yang diinduksi dengan sel darah merah domba 2 %. Hewan coba yang digunakan dikelompokkan menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Hewan coba yang akan digunakan adalah kelinci dengan berat 700 – 800 gr sebanyak 20 ekor yang akan dibagi menjadi dua kelompok yaitu kelompok perlakuan dan kontrol. Metode pemeriksaan yang digunakan adalah dengan uji heamaglutinasi dengan mengamati terjadinya aglutinasi antara serum yang diambil dari darah kelinci dengan sel darah merah domba   (SDMD) 2 %. Rata-rata titer Imunoglobulin G (IgG) pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 2.26 µl/ml dan 1.62 µl/ml. Hasil uji statistik dengan paired t test dengan tingkat kepercayaan 0,95 % menunjukkan bahwa terdapat perbedaan yang signifikan ( p < 0,05) antara kelompok perlakuan dan kelompok kontrol yaitu dengan nilai signifikannya adalah 0,043.
Efek protein rekombinan MPT64 Mycobacterium tuberculosis terhadap proliferasi sel T dan Sel B secara in vitro Fihirrudin, Fihirrudin; Inayati, Nurul; Nidhal, Lalu Unsun; Jannah, Raudatul
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.278

Abstract

Mycobacterium protein tuberkulosis (MPT) 64 merupakan protein imunogenik yang disandi oleh gen Rv1980c dan berada pada lokasi Regions of Diffrences (RD) 2. Protein MPT64 di prediksi sebagai antigen dari M. tuberculosis yang pertama kali berinteraksi dengan sistem imun tubuh dari host. Penelitian ini bertujuan untuk mengetahui proliferasi sel T dan sel B yang di induksi dengan protein MPT64 secara invitro. Penelitian ini merupakan penelitian ekperimental yaitu melakukan uji proliferasi terhadap sel T dan sel B yang di induksi dengan protein MPT64 pada media RPMI. Purifikasi protein MPT 64 dilakukan dengan kolom Protino™ Ni-NTA System. Uji proliferasi sel T dan sel B dilakukan dengan Metode MTT, dimana hasil optical dencity nya (OD) di baca dengan ELISA reader pada panjang gelombang 595 nm. Data nilai Optical dencity hasil uji MTT di analisis dengan uji statistik One Way Anova. Hasil menunjukkan bahwa Rata-rata OD proliferasi sel T yang di induksi dengan protein MPT64 10 μg/ml, 5 μg/ml, 2,5 μg/ml dan 1,25 μg/ml masing-masing adalah 0.276, 0.202, 0.184 dan 0.178. Rata-rata OD proliferasi sel B yang di induksi dengan protein MPT64 10 μg/ml, 5 μg/ml, 2,5 μg/ml dan 1,25 μg/ml masing-masing adalah 0.434, 0.380, 0.285 dan 0.251. Hasil uji statistik menunjukkan perbedaan yang signifikan pada setiap perlakuan dengan nilai probabilitas (p=0,011) <0,05. Protein MPT64 konsentrasi 10 μg/ml menunjukkan hasil yang paling optimal dalam menginduksi proliferasi sel T dan sel B secara invitro.
Description of SGPT Levels in Construction Workers Who Have an Active Smoking Habit Sapna, Eva; Inayati, Nurul; Jiwantoro, Yudha Anggit; Kristinawati, Erna
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.349

Abstract

Physical activity is body movement that requires skeletal muscles and can cause energy expenditure. Physical activity is divided into three, namely light, moderate and heavy physical activity. Construction workers who carry out heavy physical activity for 7 to 8 hours every day can lose a lot of energy and fluids as well as important micronutrients, one of which is iron. Apart from carrying out heavy physical activities, construction workers also have an active smoking habit, which means they are considered active smokers if they smoke more than 20 cigarettes every day. Smoking is something that can cause health problems and smoking has become the biggest cause of death in the world. Cigarette raw materials such as tar, nicotine and carbon monoxide are the main toxicants that can trigger the formation of free radicals. The Research objective is to determine the description of SGPT levels in construction workers who have an active smoking habit. The methods of this research design is descriptive observational research where this research was carried out using the enzymatic kinetic method a total of 28 samples based on calculations using the Slovin formula. The results of the research is examining SGPT levels in construction workers from 28 samples showed an increase in active smokers 11-15 years, namely 3 samples and active smokers 16-20 years, namely 2 samples, there was an increase or were outside the normal value, which is where the normal value of SGPT levels in men -men, namely 42 U/L. The conclusion of this research is showed that 5 of the 28 samples had increased SGPT levels with levels of 63 U/L, 58 U/L, 52 U/L and 49 U/L in 2 samples.
Comparison of Fast and Time Blood Glucose Levels in Chronic Disease Management Program Patients (Proalnic) and Non-Prolanic with Diabetes Mellitus in Praya Health Center Aini, Miftahul; Tatontos, Erlin Yustin; Inayati, Nurul; Pauzi, Iswari
THRIVE Health Science Journal Vol. 1 No. 1 (2024): March
Publisher : Balai Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56566/thrive.v1i1.92

Abstract

Glucose is the most important carbohydrate which is mostly absorbed into the bloodstream as glucose and other sugars are converted into glucose in the liver. Blood glucose levels are closely related to DM. Prolanic (Chronic Disease Management Program) is a health service system that involves participants from Health Facilities and BPJS health in the context of health care for participants suffering from chronic diseases including diabetes mellitus to achieve optimal quality of life with cost-effective and efficient health services. To determine the comparison of fasting and intermittent blood glucose levels in prolanic and non-prolanic patients suffering from DM at Praya Health Center. : This type of research is Analytical Observation, the sample is taken purposively. A sample of 30 respondents, in this study were 15 respondents who took prolanis and 15 respondents who did not follow prolanis who had a history of diabetes mellitus and underwent blood glucose level checks at the UPTD BLUD Laboratory of Praya Health Center. Based on research that has been done, the average fasting blood glucose level in prolanis is 158 mg/dl and fasting glucose is 204 mg/dl. Meanwhile, non-prolanic fasting glucose levels are 156 mg/dl and fasting glucose levels are 204 mg/dl.
Efektivitas Pembiasaan Salat Duha Dalam Pencapaian Nilai-nilai Profil Pelajar Pancasila di SMP Negeri 13 Surabaya Fanani, Miftakhul; Fahmi, Muhammad; Muhammad Hafidh Ubaidillah; Inayati, Nurul; Fitriyah, Latifatul
TARBIYA ISLAMIA : Jurnal Pendidikan dan Keislaman Vol. 14 No. 1 (2024): TARBIYA ISLAMIA
Publisher : Islamic Education Department, Islamic University of Majapahit (Universitas Islam Majapahit) Mojokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36815/tarbiya.v14i1.2300

Abstract

Duha prayer is one of the habituation activities before learning which is carried out at SMP Negeri 13 Surabaya. The habit of praying Duha prayer is carried out with the aim that students are accustomed to carrying it out regularly, both at home and at school, and also aims to form religious character in students. With the Duha prayer, it is hoped that it can grow characters that are following the elements of the Pancasila student profile. This study was carried out to know the effectiveness of the habituation of the duha prayer carried out at SMP Negeri 13 Surabaya. A descriptive quantitative method was used with questionnaires and interviews to collect data from students and teachers regarding the habit of praying duha. The results showed that the habit of praying duha prayer was more effective than before, namely at the beginning of the study. Although it can be said to be effective, the habit of praying duha still needs to be improved so that its effectiveness can be increased even more.
Gambaran Nikotin pada Pra Lansia Perokok Linting (Tembakau) di Desa Pohgading Timur Kecamatan Pringgabaya Isnaeni, Robiatul; Inayati, Nurul; Anggit jiwantoro, Yudha; Kristinawati, Erna
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.40

Abstract

Background: Cigarettes are a type of tobacco product produced from the Nicotiana Tabacum, Nicotiana Rustica and other species. Cigarettes are cylinder-shaped from paper measuring between 70 – 120 mm long with a diameter of around 10 mm containing chopped tobacco leaves. There are two types of cigarettes, namely hand-rolled cigarettes and electronic cigarettes which are usually called vape or vapor. Objective: To find out the description of nicotine in hand-rolled (tobacco) smokers in East Pohgading village, Pringgabaya subdistrict. Method: The research is a descriptive analytical method. By taking samples using purposive sampling technique. Results: The results of the research that was carried out obtained samples of 42 elderly people who used hand-rolled cigarettes (tobacco), 41 samples were positive and 1 was negative for nicotine. Conclusion: Results from the urine of pre-elderly respondents who smoked hand-rolled (tobacco) were positive for nicotine as many as 41 people and 1 person was negative.
Pengaruh Konsumsi Timun dan Bawang Merah terhadap Penurunan Kadar Kolesterol Pasien di Wilayah Kerja Puskesmas Babakan Nandini, Ni Kadek; Zaetun, Siti; Inayati, Nurul; Lale Budi Kusuma Dewi
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.44

Abstract

Hypercholesterolemia is caused by the liver not being able to control LDL in the blood vessels, this condition will cause atherosclerosis and cardiovascular disease. Sterols and flavonoids are content that found in cucumbers which can lower cholesterol by replacing sterols with LDL which will be absorbed in the digestive system. The LDL will be excreted in the form of feces with the help of flavonoids And quercetin is a content found in red onions that can reduce cholesterol levels in the blood by inhibiting the HMG Co-A reductase enzyme. Yankestrad is a program at the Babakan Public Health Center include the use of medicinal plants (TOGA) such as cucumbers and red onions. The purpose of this study is to know the effect of cucumber and red onions consumption on reducing cholesterol levels in patients in the Babakan Health Center work area through the Yankestrad program. Research methods are analytical observational with case control research design and purposive sampling methods. The results of this there is an effect of consuming cucumber and red onions on reducing cholesterol levels.
ANALISIS GAMBAR STIMULUS PADA PERMULAAN BAB BUKU AJAR PAI KELAS VIII PRESPEKTIF VISUAL DAN PENDIDIKAN Inayati, Nurul; Masithoh, Arina Dewi
PARAMUROBI: JURNAL PENDIDIKAN AGAMA ISLAM Vol 7 No 1 (2024): PARAMUROBI: JURNAL PENDIDIKAN AGAMA ISLAM
Publisher : Program Studi Pendidikan Agama Islam Fakultas Ilmu Tarbiyah dan Keguruan UNSIQ Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/paramurobi.v7i1.7018

Abstract

Analysis of stimulus images at the beginning of the 8th grade Islamic Religious Education (PAI) textbook chapter highlights the important role of visuals in the context of religious learning. This research adopts a qualitative perspective with a focus on content, semiotic, and visual analysis to understand how these images influence students' perceptions and facilitate deep learning. Through this approach, we reveal the various messages, themes and values ​​implicit in these images, as well as how visual elements such as composition, color and symbolism are used to convey Islamic religious concepts. The results of this research provide valuable insight into the potential of stimulus images as an effective tool in enriching students' learning experiences in religious subjects, as well as highlighting the importance of integrating visual aspects in the religious education process. The practical implications of these findings can enrich PAI learning designs that are more responsive to students' needs and preferences in visual contexts.
Efisiensi Evaluasi Non Tes Melalui Microteaching Dalam Mata Kuliah Pendidikan Sejarah Peradaban Islam Al-Bahiyyah, Maharanin Nisa’; Yumna, Haniya Fatkhul; Zakhrofa, Zulfa; Inayati, Nurul
IQRO: Journal of Islamic Education Vol. 6 No. 2 (2023): DESEMBER 2023
Publisher : Prodi Pendidikan Agama Islam FTIK IAIN Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24256/iqro.v6i2.4744

Abstract

Evaluasi diperlukan dalam suatu pembelajaran. Untuk meningkatkan kualitas pendidikan dibutuhkan sistem evaluasi yang tepat agar mencapai tujuan dari kualitas pembelajaran yang direncanakan. Evaluasi dapat dilakukan melalui berbagai metode, dalam mata kuliah PSPI menggunakan evaluasi non tes berupa micro teaching. Tujuan dalam penelitian ini yaitu untuk mengkaji hasil dari micro teaching dalam mata kuliah Pendidikan Sejarah Peradaban Islam sebagai alat evaluasi non tes yang efisien dalam meningkatkan kemampuan mengajar mahasiswa dalam mata kuliah Pendidikan Sejarah Peradaban. Metode yang digunakan dalam penelitian ini adalah metode kualitatif dengan studi lapangan. Adapun teknik pengumpulan data dalam penelitian ini menggunakan teknik observasi, wawancara dengan dosen pengampu mata kuliah dan beberapa mahasiswa. Hasil dari penelitian ini menunjukkan bahwa praktik micro teaching juga memiliki manfaat yang tak kalah banyak walaupun mahasiswa masih mengalami beberapa kendala dalam praktik micro teaching. Demikian pula evaluasi antar teman mengajar memiliki dampak positif yang signifikan karena berpotensi bagi mahasiswa untuk mengetahui kelebihan dan kekurangan sebagai pengajar dan mengurangi tekanan mahasiswa yang biasanya didapatkan dari dosen pengampu karena yang menjadi evaluator adalah teman sejawat. Maka dengan hal itu menunjukkan bahwa praktik micro teaching dan memberikan evaluasi yang berulang disetiap pertemuan mata kuliah berhasil meningkatkan kualitas kemampuan mengajar mahasiswa dalam mata kuliah Pendidikan Sejarah Peradaban Islam.
Comparison of Results of Atypical Lymphocyte Test, RT-PCR and ELISA Using Recombinant Multivalent Envelope Protein Domain III (ED-III) Dengue Virus in Dengue Fever Patients Inayati, Nurul; Fihiruddin, Fihiruddin; Ernawati, Sri; Unsunnidhal, Lalu; Jannah, Raudatul
JURNAL INFO KESEHATAN Vol 22 No 3 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss3.1644

Abstract

Prevention of the transmission of dengue hemorrhagic fever (DHF) is carried out by breaking the chain of dengue transmission and administering vaccines, but to date, this has not achieved the expected target. Dengue virus tests using RT-PCR require skills and relatively expensive equipment. Serological test of IgM and IgG often shows false negatives or false positives, especially in dengue-endemic areas. The antibody test against NS1 using the ELISA method has weaknesses because anti-dengue IgM is often not detected in secondary infections. The development of serodiagnostic tests for rapid, affordable, sensitive, and specific detection of dengue virus infection is very necessary. Recombinant multivalent envelope proteins domain III (ED-III) dengue virus is a biomarker that has the potential to be developed to detect all dengue virus serotypes. One of the proteins that has high antigenicity is glycoprotein E which is found in the envelope of the dengue virus and is the most antigenic part of the virus. This research aims to combine several parts of the antigenic protein found in all dengue virus serotypes as immunoserodiagnostic material. This research is an analytical survey research, that compares the results of the atypical lymphocyte test, RT-PCR, and ELISA using the multivalent ED-III antigen. The number of samples used was 26 samples obtained from patients who were diagnosed with dengue fever using an accidental sampling technique. The results of the atypical lymphocyte examination showed 14 positive samples, while the results of the RT-PCR and ELISA examinations were 23 and 24 positive respectively. The average Optical density (OD) of examination using the ELISA method was 1.902 with sensitivity and specificity levels of 92% and 96%. There is no difference result of the RT-PCR compared with the ELISA test. Therefore, recombinant multivalent envelope protein domain III (ED-III) dengue virus can be used as a diagnostic tool to detect dengue fever infection.