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Sri Untari
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Pengaruh Waktu Ekstraksi dan Konsentrasi Ekstrak Rambut Jagung (Corn Silk) Terhadap pH, Total Fenol Dan Aktivitas Antibakteri Haslina Haslina; Sri Untari
Jurnal Pengembangan Rekayasa dan Teknologi Vol. 1 No. 2 (2017): November (2017)
Publisher : Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/jprt.v13i2.933

Abstract

The growth of microorganisms in foodstuffs can lead to profitable changes such as nutritional improvements in food, digestibility or storage. In addition, the growth of microorganisms in food can also lead to undesirable physical and chemical changes, so the food is not worth consuming. Staphylococcus aureus and Escherichia coli are the bacteria that often cause infection in humans. To prevent this it is necessary to have bioactive components that can inhibit the growth of microorganisms. Active compounds such as saponins, triterpenoids, phenols, tannins, flavonoids and alkaloids are known to have antibacterial activity. Several studies have reported the chemical content found in corn hair. Corn hair is part of a corn plant that has not been used effectively because it is considered as waste. Based on research, corn hair contains proteins, vitamins, carbohydrates, calcium salts, potassium, magnesium, and sodium, essential oils, steroids such as sitosterol and stigmasterol, alkaloids, saponins, tannins, and flavonoids. So presumably corn hair extract has antibacterial activity.This study aims to determine the effect of extraction time and concentration of corn hair extract on pH, total phenol and antibacterial activity.The experimental design used in this study was Randomized Block Design (RBD) with 3 replications. The treatments used were Extraction Time A1 = 30 minutes, A2 = 40 minutes, A3 = 50 minutes and Concentration Extract (material: solvent) K1 = 75% (w / v), K2 = 50% (w / v), K3 = 25% (w / v). Furthermore, the data obtained were analyzed variant and if there was any difference between treatments followed by Duncan Multiple Range Test (DMRT) test at 5% level. The results showed that all treatments produced bioactive compounds and formed inhibit zone of each test bacteria. The best time to obtain an antibacterial antibacterial extract on corn hair is 50 minutes. This was supported by the lowest pH average of 5.1 and the highest mean total phenol obtained from treatment A3 (50 minutes) was 7288,64 μgGAE / g and at 75% concentration formed the largest bacterial inhibition zone in each test bacteria.
Co-Authors Achmad Mufti Fauzi Ade Eka Anggraini Alfan Bramantya Annisa Izzah Dinillah Annisa Purnadita Arief Gunawan Brilian Asa Margantara Cindy Putri Octivia Dedik Setyawan Dewik Wulan Prihatiningsih DHIYAN NANY WIGATI Diah Aulia Azizatur Rohmah Didik Sukriono Dinda Mifta'ul Qolifah Dwi Setyowati Elsa Lutfiyah Endang Sri Jatmikowati Ersya Savira Maharani Eva Dina Bella Septina Farisma Eka Avaria Fatimah Siti Umairoh Fauziyatul Khoiroh Febriana Tri Kusumawati Firman Rahmad Hafidz Fitrotun Nafsiyah Hafizh Naufalian Haslina Haslina Hesti Dwi Prihastuti Iman Yaqin Indah Novitasari Nasution Irma Nusandari Jayusman Jayusman Karina Ais Sitoresmi Kasmin Naenggolan Kumalasari, Nurya Latifah Istidhamah Mar'atul Azizah Marcella Cristi Ananda Masruroh, Riris Meity Mulya Susanti Muchtar Lutfie Muhammad Aries Soetiono Muhammad Makmun Nada Silmia Millati Nany Wigati, Dhiyan NUR FATIMAH Nurul Kodiyah Pujiati, Eko Radeni Sukma Indra Dewi Rahayu, Dwi Puspa Rahmawati Ratna Hayu Riniasih, Wahyu Riska Indrayati Riska Indriyati Riza Nur Rahmat Sahara, Rizki Shirly Rizki Kusumaningrum Shofiyatul Azmi Siregar, Julinda Sita Azizah Soediman Sastrodihardjo Sri Martini Sri Temu Sumiyanti Sunaryo Sunaryo SURYANI Susanti, Meity Mulya Sutiyono Syamsul Ph.D M.A. Hadi Widari Widari Widy Nurwiandani wiwin hindriyawati