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All Journal Jurnal Biologi Udayana KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Buletin Plasma Nutfah Prosiding Seminar Biologi Journal of Natural Sciences and Mathematics Research Jurnal Pendidikan Biologi Biosfer: Jurnal Pendidikan Biologi AL-HAYAT: Journal of Biology and Applied Biology Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Jurnal Puruhita Life Science Gunung Djati Conference Series Filogeni: Jurnal Mahasiswa Biologi Jurnal Dehasen Mengabdi Komunitas: International Journal of Indonesian Society and Culture
Baiq Farhatul Wahidah
Universitas Islam Negeri Walisongo Semarang
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Journal : Gunung Djati Conference Series

 1 
Optimasi Suhu Annealing Marka ISSR Sebagai Langkah Awal Dalam Pengejawantahan Keragaman Genetik Ficus fistulosa dan Ficus variegata Risqi Aprilianingsih; Baiq Farhatul Wahidah; Muhammad Rifqi Hariri
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Ficus is a genus with the largest number of members of the Moraceae tribe which are often found in Indonesia and has a significant role in the tropics as a keystone species. Ficus has a fairly wide distribution with several members of which F. fistulosa and F. variegata can grow in primary forests to urban areas such as Mount Botol, Mount Halimun Salak National Park and Bogor City. Differences in growing locations allow for differences in genetic diversity in plants so that information needs to be explored and disclosed. This study aims to determine the optimum temperature at the annealing stage and to select primers with the best amplicon as the first step to reveal the genetic diversity of F. fistulosa and F. variegata. The molecular marker used is inter simple sequence repeats (ISSR) which is a molecular marker with a very high level of polymorphism and is quite often used to reveal genetic diversity in plants. A total of four samples of F. fistulosa and F. variegata and 10 ISSR primers were used in this study. Modifications in the amplification stage can be done by changing the temperature used based on certain intervals. A good amplicon is influenced by the optimum temperature conditions at the annealing stage when amplification is carried out. Seven ISSR primers were successfully amplified at an annealing temperature of 52 C, while the other two primers were amplified at an annealing temperature of 53 C. The ISSR 5 primer could not be amplified even when the third annealing temperature modification was carried out, so it was not used in the next study. Nine ISSR primers with known optimum temperatures can be used in the study of embodiment of genetic diversity of Ficus spp. in the Bogor City and Mount Botol areas.
Co-Authors Abadiyah, Auliya Saadatul ACHMAD FARAJALLAH Adi Al-Fauzy Adi Al-Fauzy, Adi Adi Sutanto Aidatul, Agustina Alfina, Lina Anif Rizqianti Hariz Aprilianingsih, Risqi Apriliyani, Riski Arifin Surya Dwipa Irsyam Asma Lutfi, Asma Asri Febriana Asri Febriana, Asri Atika Wijaya Auliya Saadatul Abadiyah Azizah, Ismatul Birran, Regan Abadi Khalid Putra Yusac Brintan Yonaka Dhea Dani Cut Muthiadin Devi Octavia Dhea Dani, Brintan Yonaka Dinalhaq, Putri Cahya Dinalhaq, Putri Cahya Dyah Perwitasari DYAH PERWITASARI -FARAJALLAH Eri Sulis Hardianto Erna Afdal Fadly Husain FATIMA, SITI Fatmawati Nur Firman Adi Saputra Firman Adi Saputra, Firman Galih Kholifatun Nisa’ Harto Wicaksono, Harto Hasby Ash Shidiqy Hasrul Hasrul Hasyimuddin Hasyimuddin Hidayat, Saifullah I Putu Gede P. Damayanto I Putu Gede P. Damayanto Irsyad Kamal Irsyam, Arifin Surya Dwipa Ismail Ismail Ismail, Ismail Isna Rasdianah Aziz Isna Rasdianah Aziz Isna Rasdianah Aziz Khasanah, Rizkiati Khoirurrais, Muhamad Kuncoro Bayu Prasetyo Lailatul Qodriyah Lala Nita Sari Lianah Lianah, Lianah Masriany, Masriany Megawati Bohari Megawati Bohari, Megawati Miswari Miswari Miswari Miswari, Miswari Muhamad Khoirurrais Muhammad Hasiibun Nuhaa Muhammad Rifqi Hariri Muhammad Rifqi Hariri Mulyanah, Wiwin Murhadi Murhadi Mustakim M, Mustakim Mustakim Mustakim Nawang, Mugi Niken Kusumarini Niken Kusumarini, Niken Nuhaa, Muhammad Hasiibun Nur Hayati Nur Hayati Nur Hayati Nurkhalis A Gaffar Nurlaila Sari Nurlailah Mappanganro Nurman Nurman Nurul Muhlisyah Nurul Muhlisyah Rahma Ziyan Firdausia Rahmat Fajrin Alir Rini Iswari Risqi Aprilianingsih Rizkiati Khasanah Rusmadi Rukmana Rusmadi Rusmadi Rusmadi Rusmadi Sary, Dian Puspita Shidiqy, Hasby Ash Sri Mulyani Sri Mulyani St Hasbiah St Hasbiah Syaifudin, Andang Ulya, Fadhliyatul Zahro, Shofiyyatuz Zul Janwar Zulkarnain Zulkarnain