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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS JURNAL HUBUNGAN INTERNASIONAL Folia Medica Indonesiana Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap
Ngurah Intan Wiratmini
Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Other

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PENDAMPINGAN PENGADAAN BAHAN PRAKTUKUM ZOOLOGI DAN BIOTEKNOLOGI BAGI GURU SMA DI KABUPATEN KLUNGKUNG N.I. Wiratmini; A.A.S. Sukmaningsih; I. Narayani; M. Pharmawati
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.349 KB) | DOI: 10.24843/BUM.2022.v21.i03.p04

Abstract

Advances in molecular biology has brought a new branch of multidiscipline study named biotechnology. To comprehend the complex nature of concepts in biology, practical and hands-on approaches are often required. This activity was a collaboration between Biology Teachers Working Group (MGMP Biologi) in Klungkung Regency with university as the facilitator, and aimed to train senior high school biology teachers in compiling learning modules as well as supplying them with the appropriate materials required in simple practical sessions, such as those in animal physiology and reproduction, as well as biotechnology. The event was held successfully, attended by total of 20 biology teachers from six senior high schools (SMA) in Klungkung District, Klungkung Regency, started by introductory lectures and followed by practical sessions on how to perform mice vaginal and sperm smear, human blood smear, and DNA extraction of plant tissues. Keywords: practicals, zoology, biotechnology, senior high school students
Kuantifikasi Protein HAS2, PTX3, BCL2 dan BAX dalam Sel Kumulus Oosit Matur (MII) dan Imatur (MI) Pada Pasien Bayi Tabung Ni Putu Sri Risa Dewi; Ngurah Intan Wiratmini; Jaqueline Sudiman
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p01

Abstract

Oocyte developmental competence is one of the determining factors that in?uence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis of oocyte cumulus cells (CCs) can be used as a method to predict oocyte competence and viability that could potentially function as molecular predictors for IVF program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 and BAX) that are expressed in human CCs between mature (MII) and immature (MI) oocytes. This research is an analytic observational study. Twelve samples of CCs from mature (MII) and immature (MI) oocytes were collected after ovum pick up from patients undergoing ICSI. CCs samples were isolated using an ultrasonicator and protein expressions were quantified using ELISA method. The Mann-Whitney test was used to compare the protein expressions from CCs between mature and immature oocytes. The results showed HAS2, PTX3 and BCL2 proteins expressions was increase in CCs from mature oocytes but had no significant effect compared to CCs from immature oocytes, however, BAX protein expression was significantly higher (P<0.05) in CCs from immature oocytes which CCs from mature oocytes. This present study shows that BAX proteins could be used as markers for oocyte quality and maturation. Key words: ELISA, maturation, proteins marker, cumulus cells (CCs)
KLASIFIKASI TINGKAT KEMATANGAN GONAD DAN PEMIJAHAN IKAN TONGKOL KOMO BETINA (EUTHYNNUS AFFINIS (CANTOR, 1849)) YANG DIDARATKAN DI KEDONGANAN- BALI arnenda, gussasta levi; Wiratmini, Ngurah Intan; Wijana, I Made Sara
BAWAL Widya Riset Perikanan Tangkap Vol 15, No 3 (2023): (DESEMBER) 2023
Publisher : Politeknik Kelautan dan Perikanan Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.15.3.2023.132-146

Abstract

Wilayah Pengelolaan Perikanan Republik Indonesia di Samudera Hindia dengan kode WPP-RI 572 dan 573 kaya akan potensi sumber daya ikan yang didominasi oleh ikan Tongkol Komo (Euthynnus affinis (Cantor, 1849). Pemanfaatan ikan Tongkol Komo telah melampaui batas optimal dengan tekanan penangkapan yang tinggi. Biologi reproduksi Tongkol Komo perlu diketahui untuk memastikan keberadaannya di alam. Tujuan dari penelitian ini adalah Tahap Kematangan Gonad (TKG) dan pemijahan. Penelitian dilaksanakan pada bulan Februari, April, Juni hingga Desember 2020. Pengumpulan data dilakukan melalui pengamatan langsung di Pangkalan Pendaratan Ikan (PPI) Kedonganan. Teknik pengambilan sampel yang digunakan dalam penelitian ini adalah purposive stratified sampling. Analisis histologi di Laboratorium Penelitian Perikanan Tuna, Denpasar. Hasil yang diperoleh bahwa pola pertumbuhan tuna komo didominasi oleh pola alometrik positif dengan sebaran panjang 20-65cmFL. Ukuran pertama kali matang gonad (Lm) untuk betina 44,07 cmFL. Tingkat Kematangan Gonad (TKG) pada klasifikasi makroskopis dan mikroskopis perkembangan gonad didominasi oleh ikan yang belum matang gonad. Musim pemijahan pada bulan Agustus dan Desember.
Estrous cycle and uterine weight of ovariectomized menopause female rat models after treatment with Leucaena leucocephala (Lam.) de Wit leaf extract Ngurah Intan Wiratmini; Anak Agung Sagung Alit Sukmaningsih; Iriani Setyawati
Folia Medica Indonesiana Vol. 60 No. 2 (2024): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v60i2.53740

Abstract

Highlights:1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats.2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock.   Highlights:1. This study found that Leucaena leucocephala leaf ethanol extract has the ability to stimulate the growth of vaginal epithelial cells in ovariectomised rats.2. Leucaena leucocephala leaf extract can be developed as a safe and cost-effective natural alternative for hormone replacement therapy in premenopausal women and livestock.   Abstract Hormone replacement therapy has adverse effects that may cause additional health problems, such as hot flashes, cancer, ischemic stroke, and death. Phytoestrogen is an alternative ingredient used for hormone replacement therapy with less risk. The Leucaena leucocephala (Lam.) de Wit plant is widely used as a medicinal ingredient and animal feed. This study aimed to determine the effect of Leucaena leucocephala leaf extract on the uterine weight and estrous cycle of ovariectomized rats. This research used a completely randomized control design, which included two treatment groups (P1 and P2) and two control groups (negative (K-) and positive (K+)) with six replications in each group (n=24). The negative control (K-) was given 2 mL of corn oil, while K+ received 0.1 mg/kg bw of 17β-estradiol. P1 and P2 were administered 250 and 300 mg/kg bw of Leucaena leucocephala leaf extract, respectively. The extract was administered daily by gavage for 30 days. Estrous cycle data were obtained on day 31 by previously collecting vaginal swabs twice a day for 15 days. After the rats were sacrificed under anesthesia using chloroform, they underwent surgery to remove the uterine organs for weight measurement. The data were analyzed for normality and homogeneity using the Shapiro-Wilk and Levene tests. The data distribution were not normal, so the Kruskal Wallis test was used to test the hypothesis. If there was a difference between controls and treatments, the analysis continued with the Mann-Whitney test (p<0.05). The controls and treatments exhibited a significant difference in the length of each phase and overall estrous cycle. The post hoc Duncan test revealed that the highest uterine weight was found in 17β-estradiol treated rat. It was significantly different with both extracts treated rats. Both extract effect on uterine weight were not significantly difference. In conclusion, administering Leucaena leucocephala leaf extract results in an increased uterine weight and a normal estrous cycle in ovariectomized rats.
Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis Intan Wiratmini, Ngurah; Pharmawati, Made; Sukmaningsih, Anak Agung Sagung Alit; Joko Raharjo, Sentot; Widowati, Wahyu
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.287-299

Abstract

Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze its phytochemical composition, and validate its potential anti-inflammatory mechanisms through molecular docking studies. The extract was analyzed for its phytochemical composition using LC-MS, revealing the presence of sesquiterpene glycosides, flavonoid glycosides, and tannins among the identified compounds. Cell viability tests showed that the extract was not toxic to the cells at concentrations up to 50 µg/ml. Treatment with the extract significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-1β in LPS-induced RAW 264.7 cells, with the most effective concentration being 50 µg/ml. Molecular docking studies further supported the anti-inflammatory potential of the extract by demonstrating its ability to inhibit COX-2 and TNF-alpha. These findings suggest that P. niruri leaf extract has promising anti-inflammatory properties and needs further investigation as a potential treatment for inflammatory diseases. The in silico study provided insights into the molecular interactions between the extract's compounds and inflammatory targets, supporting its anti-inflammatory mechanism of action.
Diameter Lumen Trakea dan Bronkus Tikus (Rattus novergicus) yang Diberi Infusa Bunga Telang (Clitoria ternatea L.) dan Paparan Asap Rokok Elektrik Apriliani, Sinta; Wiratmini, Ngurah Intan; Sukmaningsih, A. A. S. A.
Buletin Anatomi dan Fisiologi Volume 10, Nomor 1, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.10.1.2025.1-9

Abstract

Rokok elektrik dikembangkan untuk mengurangi kecanduan masyarakat terhadap rokok. Rokok elektrik mengandung bahan kimia yang bersifat karsinogen. Tanaman telang (Clitoria ternatea) memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan infusa bunga telang terhadap penyempitan diameter lumen trakea dan bronkus tikus diterpapar rokok elektrik. Penelitian ini menggunakan 30 ekor tikus jantan (Rattus novergicus) dengan umur sekitar 2-3 bulan dan berat badan sekitar 150-200 g. Penelitian ini bersifat eksperimental dengan Rancangan Acak Lengkap (RAL) yang dibedakan menjadi 5 kelompok, yaitu K+ (0,6 mL liquid vape), K- (normal), (0,6 ml liquid vapor + 9 mg/200 g BB infusa bunga telang), P2 (0,6 ml liquid vapor + 18 mg/200 g BB infusa bunga telang) dan P3 (0,6 ml liquid vapor + 36 mg/200 g BB infusa bunga telang). Perlakuan diberikan selama 35 hari. Hasil penelitian dianalisis dengan uji Oneway ANOVA dan dilanjutkan dengan uji Duncan. Hasil yang diperoleh menunjukkan bahwa infusa bunga telang terbukti memiliki aktivitas antioksidan dalam mencegah terjadinya penyempitan diameter lumen trakea dan bronkus, dibuktikan dengan hasil analisis statistik yang menunjukkan adanya perbedaan yang nyata (P<0,05). Infusa bunga telang dengan dosis 36/200 mg memberikan pengaruh yang optimal dalam mencegah penyempitan diameter lumen trakea dan bronkus tikus setelah diberi paparan asap rokok elektrik. E-cigarettes were developed to reduce people's addiction to cigarettes. E-cigarettes contain chemicals that are carcinogens. The telang plant (Clitoria ternatea) has antioxidant activity. This study aims to determine the antioxidant activity of telang flower infusa against the narrowing of the diameter of the lumen of the trachea and bronchi of rats exposed to e-cigarettes. This study used 30 male rats (Rattus novergicus) with an age of about 2-3 months and a body weight of about 150-200 g. This research was experimental with a Completely Randomized Design (CRD) which divided into five treatment groups, namely K+ (0.6 mL liquid vape), K- (normal), (0.6 ml liquid vapor + 9 mg/200 g BB infuse butterfly pea flower), P2 (0.6 ml liquid vapor + 18 mg/200 g BB of butterfly pea flower infusion) and P3 (0.6 ml liquid vapor + 36 mg/200 g BB of butterfly pea flower infusion). The treatment is given for 35 days. The result of the study was analyzed with Oneway ANOVA test and continued with Duncan test. The results showed that the lumen diameter in the K+ group was smaller than in the other groups. Butterfly flower infusion was proven to have antioxidant activity in preventing narrowing of the lumen diameter of the trachea and bronchi, as evidenced by the results of statistical analysis which showed a significant difference (P<0.05). Telang flower infusion given at a dose of 36/200 mg provided an optimal effect in preventing the narrowing of the lumen diameter of the trachea and bronchi of rats after being exposed to e-cigarette smoke.
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Siti Masnunah Sri Sulastri Sukma Sushanti Wahyu Puji Lestari WAHYU WIDOWATI