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All Journal Jurnal Penelitian Saintek Agrikultura Cropsaver : Journal of Plant Protection Jurnal Fakultas Pertanian Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Kultivasi Jurnal Mikologi Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Indonesian Journal of Community Services Cel Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Noor Istifadah
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Other

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Journal : Cropsaver : Journal of Plant Protection

 1 
The potential of Spent Substrate of Oyster (Pleurotus ostreatus) dan Shiitake (Lentinula edodes) Mushrooms to Control Damping-off Disease (Rhizoctonia solani) in Tomato Lia Herawati; Noor Istifadah
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.034 KB) | DOI: 10.24198/cs.v1i2.20329

Abstract

Spent mushroom substrates are potential for controlling plant diseases. This study examined the abilities of spent substrates of Shiitake (L. edodes) and Oyster (P. ostreatus) mushrooms to inhibit the growth of R. solani in vitro and control damping-off disease in tomato seedling. The in vitro experiment examined the abilities of water extract of spent mushroom substrate non-sterile and sterile to inhibit the pathogen using poisonous food method. In the nursery experiment, substrates were mixed with the planting medium, 1:1, 1:2, and 1:3 (v/v). The result showed that the water extract of spent substrates of L. edodes and P. ostreatus inhibited the growth of R. solani by 29.1% – 40.6%. Non sterile water extract of spent substrate of P. osteratus showed highest inhibition level (40%). In tomato nursery, mixing  spent substrates of L. edodes or P. ostreatus with planting medium 1:2, and 1:3 (v/v) inhibited damping-off disease in tomato seedling by 57.5% - 100%.  Application of P. ostreatus spent substrate in the planting medium, 1:2 and 1:3 (v/v) resulted in 100% inhibition of the disease.Key words: tomato seedlings, water extract, spent mushroom substrate, in vitro
The Abilities of Bacteria and Yeast Isolated from Vermicompost Water Extract to Inhibit Alternaria solani in vitro and Early Blight Disease on Tomato Noor Istifadah; Retno Anjani Putri; Sri Hartati
CROPSAVER Vol 4, No 2 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i2.37374

Abstract

Early blight disease (Alternaria solani Sor) is one of limiting factors in tomato production. Bacteria and yeast are potential for biocontrol of plant diseases.  Sources to obtain biocontrol agens is water extract of organic matters. The objective of this study was to examine the abilities of bacteria and yeast isolated from vermicompost water extract in inhibiting the growth of A. solani in vitro and suppressing the pathogen infection in tomato fruits and leaves. Two types of vermicompost used in this study were cattle manure vermicompost and goat manure vermicompost. The isolation of bacteria and yeast from the vermicompost water extract resulted in 14 isolates, which were 10 isolates from cattle manure vermicompost and four isolates from goat manure vermicompost. Among the isolates, six isolates (three yeast and three bacterial isolates) inhibited the growth of A. solani in vitro by 42.8% – 79.1%. In tomato fruits, five isolates inhibited A. solani infection by 70.6% - 100.0%.  In tomato plants, four isolates suppressed early blight disease in tomato leaves by 56.2% - 83.5%. The isolate that showed consistent effects in vitro as well as in vivo was bacterial isolate KB 3. This isolate was potential as biocontrol agent of tomato diseases caused by A. solani.
The Potential and Application Frequency of Yeast from Tomato and Cogon Grass Leaves to Suppress Powdery Mildew Disease in Tomato Noor Istifadah; Nurul Ihsani; Sri Hartati
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.256 KB) | DOI: 10.24198/cs.v1i2.20883

Abstract

Powdery mildew (Oidium sp.) is important disease in the greenhouse tomato production. One of environmentally-friendly control method of the disease is biological control. Yeast from phyloplane of weeds such as cogon grass are potential as biocontrol agents. This study evaluated the potential of yeast isolated from the leaves of cogon grass and wild tomato to suppress powdery mildew of tomato. The samples were obtained from several areas in Sumedang and Bandung, West Java. The yeast isolates were selected on their abilities in controlling powdery mildew in tomato seedlings. The selected isolates were used for further experiment examining the effects of frequency of yeast application on the disease control. This experiment was arranged in Randomized Complete Block Design with 12 treatments and three replications. The treatments were three yeast isolates, applied once before pathogen inoculation, applied regularly every week or two weeks, the check and fungicide treatment. The results showed that among 13 yeast isolates obtained there were three isolates that suppressedthe development of powdery mildew disease by 61.0%- 68.6%. In further experiment, relatively highest disease reduction (58,6%) was showed in the application of yeast isolate from wild tomato leaves (TB1 isolate) every week.Keywords : biological control, phyloplane, Oidium sp., wild tomato
Application Methods of Compost and Vermicompost for Suppressing Damping off Disease (Rhizoctonia solani) in Tomato Seedling Istifadah, Noor; Baharudin, Aziz; Hartati, Sri
CROPSAVER Vol 7, No 1 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i1.55226

Abstract

Damping off caused by Rhizoctonia solani is an important soil-borne disease in tomato nursery. In addition to their role as organic fertilizer, compost and vermicompost are able to suppress plant diseases. Compos and vermicompost can be applied directly or in the form of their soaking water or teas. This study evaluated the abilities of compost, vermicompost, compost tea and vermicompost tea to inhibit R. solani in vitro and suppress damping off disease in tomato seedlings. The in vitro experiment used completely randomized design, while the experiment in tomato seedlings used randomized complete block design. The in vitro experiment examined nonsterile and sterile suspension or teas of compost and vermicompost. The treatments in tomato seedling experiment were compost or vermicompost applied in the growth media (25% v/v), compost or vermicompost tea applied to the tomato seedlings every 3 days or 7 days, and untreated check. The results showed that in the agar media containing suspension of compost or vermicompost nonsterile was dominated by Trichoderma spp. colonies and therefore the growth of R. solani was inhibited by 58.5-60.0%. The sterile preparation of compost, vermicompost and their teas did not inhibit the pathogen. Compost and vermicompost incorporated to the growth media suppressed damping off disease caused by R. solani by 54.2-66.7%. The abilities of compost and vermicompost to suppress the disease and support plant growth were better than their teas. Drenching the seedlings with compost tea every 3 or 7 days suppress damping off disease by 31.0-41.7%. However, vermicompost tea showed disease suppression (27.6-37.1%) only if it was applied every 3 days.
The Abilities of Bacillus subtilis and Trichoderma sp. to Suppress Powdery Mildew Disease on Tomato Leaves Istifadah, Noor; Maharani, Febry Aulia Riski
CROPSAVER Vol 6, No 2 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i2.49011

Abstract

Powdery mildew caused by Oidium sp. is an important disease on tomatoes cultivated in the greenhouse. Biological control is an environmentally-friendly method for plant disease control. The objective of this study was to examine the abilities of Bacillus subtilis and Trichoderma sp. isolated from compost tea to suppress powdery mildew disease on tomato leaves. The experiments were arranged in randomized complete block design with seven treatments consisting application of B. subtilis, Trichoderma sp., the combination of both microbes, molasses (1%), water and control (untreated leaves). In the first experiment, the treatments were applied on the infected leaves as curative strategy, while in the second experiment the treatments were applied at 7, 4, and 1 day before and every 3 days after pathogen inoculation. The results showed that B. subtilis, Trichoderma sp. or combination of both microbes that were applied in the infected leaves only reduced the disease severity by 29–33%. However, the application of B. subtilis every three days started at 7 days before pathogen inoculation suppressed powdery mildew by 93.7%, whereas combination of B. subtilis and Trichoderma sp. reduced the symptom on inoculated leaves by 85.3%. Spraying tomato leaves with 1% molasses reduced the infection rate and suppressed powdery mildew by 78.6%. Mixing the molasses with Trichoderma sp. suspension (10%, v/v) did not improve the level of suppression. This study showed that application of B. subtilis regularly as preventive measure is very potential for biological control of powdery mildew disease.
Inhibition Effects of Culture Filtrates and Volatile Compounds of Antagonistic Microbes Isolated from Vermicompos and Compost Teas on the Growth of Alternaria solani Sor. in Vitro Istifadah, Noor; Septiandini, Adelia; Hartati, Sri; Widiantini, Fitri
CROPSAVER Vol 5, No 2 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i2.43278

Abstract

Alternaria solani Sor. is one of destructive pathogens in solanaceous plants including tomato. Bacteria and yeast isolated from water extract of organic matters are potential as biological control agents of plant pathogenic fungi. Mechanisms of antagonism of bacteria and yeast can be through antibiosis. This study was conducted to examine the abilities of culture filtrate and volatile compounds produced by antagonistic bacteria and yeast isolated from compost and vermicompost teas to inhibit the growth of A. solani in vitro. The experiments were arranged in randomized complete design with four replications. The culture filtrate experiment applied well diffusion method, while the volatile compound effect experiment used petri dish sandwich method. The results showed that the culture filtrates of four bacteria and three yeast isolates inhibited the growth of A. solani in vitro by 16.6-87.5%. The highest inhibition level was showed by KSB4 isolate (Bacillus subtilis), a bacterial isolate from cow manure compost tea. In the volatile compound effect experiment, the tested bacteria and yeast isolates inhibited the pathogen growth by 31.3-75.2%, with the highest inhibition was showed by KcB3, a bacterial isolate from vermicompost tea. The isolate that its culture filtrate and volatile compounds both showed high inhibition level (62.7% and 87.5%) on A. solani growth was KSB4 isolate (B. subtilis).
Capability of Three Yeast Species in Suppressing Green Mold (Penicillium digitatum) on Siam Citrus Fruit (Citrus nobilis) Hartati, Sri; Utari, Elinda Dwi; Rasiska, Siska; Istifadah, Noor
CROPSAVER Vol 5, No 2 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i2.42173

Abstract

Green mold is one of the main diseases in citrus fruits caused by Penicillium digitatum. One of the managements that can be used to control this disease is by using yeasts as antagonistic agents. The objective of this study was to evaluate the ability of yeast to suppress Green mold caused by P. digitatum in siam citrus (Citrus nobilis Lour.). The study was conducted using a completely randomized design. The treatments were the application of yeast, consisting of Aureobasidium pullulans isolate Dmg 11 DEP, Rhodotorula minuta isolate Dmg 16 BE, Candida tropicalis isolate Lm 13 BE, fungicide benomyl, and control. The results showed that A. pullulans isolate Dmg 11 DEP, C. tropicalis isolate Lm 13 BE, and R. minuta isolate Dmg 16 BE were able to suppress the growth of P. digitatum in vitro on dual culture method, with suppression levels ranged from 14.64%-21.02%. The greatest suppression was caused by R. minuta isolate Dmg 16 BE. The formation of volatile compounds test results were ranged from 14.51%-34%, and the highest suppression was on C. tropicalis isolate Lm 13 BE. Application of the yeasts on siam citrus fruit was able to reduce the pathogen’s growth, and the suppressions were 28.87% to 68.72%. R. minuta isolate Dmg 16 BE caused the highest suppression.
Co-Authors Agus Susanto Ai Melawati Anggi Ayuningtyas ANNE NURAINI ANNE NURBAITY Aoliya, Salwa Rohmatul Arisaputri, Annisa Azis Muslim, Azis Baharudin, Aziz Ceppy Nasahi Danar Dono Dian E. Kkartiwa DIYAN HERDIYANTORO Endah Yulia Yulia Erni Suminar Farida Fitri Widiantini Hersanti - Hilda Sandra Utami I P Sari Kusumiyati Lia Herawati Luciana Djaya Luciana Djaya, Luciana Maharani, Febry Aulia Riski Maulana, Raihan Rahmat Mochamad Arief Soleh, Mochamad Arief Muhamad Salman Umar Nasrul Hakim Noladhi Wicaksana Nurdiana, Dadi Nurjihan, Khansa Nurul Ihsani Octa Saktianti P. Rizky D. Sianipar Prabowo, Yudhisa Hendri Putu Ghita Novilaressa Rahmat Budiarto Retno Anjani Putri Rika Meliansyah Rohmah, Nanda Dea Nikmatu Saleh, Teddy Wahyana Salsabila, Haifanisa Santa Monica Septiandini, Adelia SIska Rasiska, SIska Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Rosmala Dewi Sudarjat Sudarjat Suseno Amien SYARIFUL MUBAROK Tarkus Suganda Toto Sunarto Tri Mayanti Utari, Elinda Dwi Wahyu Daradjat Natawigena Wawan Sutari