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PENDAMPINGAN PENGOLAHAN LIMBAH ORGANIK MENJADI ECOENZYME BAGI GURU-GURU SMP SEKABUPATEN MAGELANG Yosephine Louise, Isana Supiah; Rohaeti, Eli; Aznam, Nurfina; Arianingrum, Retno; Budiasih, Kun Sri; Mercuriani, Ixora Sartika; Rinawati, Wika; Fitriyana, Nur
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol. 8 No. 2 (2024): Vol 8, No 2 (2024)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpmmp.v8i2.71193

Sampah rumah tangga umumnya berupa sampah organik, demikian juga limbah/sampah pedagang lotis/rujak, jus, sayur dan buah, yang umumna hanya dibuang begitu saja, menumpuk dan menyebabkan aroma tidak sedap dan menyebabkan sarang lalat, yang berakibat terhadap kesehatan masyarakat sekitar. Untuk itu perlu diupayakan pengolahan limbah rumah tangga, penjual lotis/rujak, jus, sayur dan buah yang berupa limbah/sampah organik menjadi produk yang bermanfaat dan memiliki nilai ekonom. Pada kegiatan pendampingan guru-guru SMP se-Kabupaten Magelang dengan dana dari Program MF (Matching Fund) 2023 (Ristekdikti) telah dikenalkan produk ecoenzim sebagai produk fermentasi limbah/sampah organik, yang memiliki banyak manfaat, antara lain, pupuk, insektisida, dan memiliki khasiat obat (obat luar). Selama kegiatan pendampingan, peserta tidak hanya dikenalkan produk ecoenzim secara teori, tetapi langsung mempraktekkan pembuatan ecoenzim, dengan bahan-bahan dan peralatan yang telah disediakan. Peserta sangat menikmati dan sangat bersemangat selama pendampingan, secara umum peserta menyatakan bahwa kegiatan ini sangat bermanfaat, penyampaian materi dapat diterima dnegan sangat baik, metoda yang digunakan sangat sesuai dan kegiatan ini sangat membantu membuka wawasan peserta pendampingan.

Desain Primer Untuk Isolasi Gen L-LDH Berdasarkan Genom Streptococcus pluranimalium dan Aplikasinya Rifqi, Daffa Muhammad; Mercuriani, Ixora Sartika
Kingdom: The Journal of Biological Studies Vol 10, No 1 (2024): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v10i1.18581

Asam laktat diketahui merupakan molekul dengan manfaat positif untuk manusia. Molekul tersebut dapat diproduksi oleh bakteri asam laktat. Produksi asam laktat seringkali terganggu karena beberapa spesies bakteri perlu penyesuaian khusus pada substrat, sehingga salah satu solusinya adalah kloning gen atau over-expression enzim L-LDH pada bakteri kompeten. Salah satu langkah prosedur tersebut adalah isolasi gen. Desain primer dilakukan menggunakan aplikasi Benchling pada subjek genom Streptococcus pluranimalium TH11417 dan seluruh primer diujikan secara in-silico menggunakan aplikasi UGENE dan in-vitro menggunakan metode Polymerase Chain Reaction (PCR). Empat pasang primer dihasilkan dengan rincian LSPai 1178, LSPbi 993, LSPad 765, dan LSPbd 846. Primer berhasil menempel dan menghasilkan amplikon yang sesuai pada uji in-silico menggunakan templat genom S. pluranimalium TH11417, namun hanya LSPai 1178 dan LSPad 765 yang dapat menempel pada templat genom S. pluranimalium 14A0014. Primer tidak dapat menempel pada uji in-vitro dikarenakan kontaminasi isolat bakteri templat DNA. Kontaminasi dikonfirmasi melalui analisis sekuen 16s rRNA dan didapatkan bahwa amplikon 16s rRNA isolat bakteri uji sesuai dengan sekuen 16s rRNA Bacillus cereus. Sekuensing basa amplikon uji in-vitro primer LSPai 1178 menunjukkan bahwa amplikon bukan merupakan bagian dari sekuen target.

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.

Inducing An Axillary Bud of Dendrobium Red Emperor ‘Prince’ With An Addition of Bap in Vitro Septasari, Mistri; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.216

The purpose of this study is to determine the effect of the addition of BAP and the position of nodus on the formation of axillary shoots and to know the optimum concentration of BAP for the induction of Dendrobium Red Emperor 'Prince' axial shoots. The research design used is a Complete Factorial Randomized Design consisting of one treatment, namely: concentration of growing regulatory substances (ZPT) BAP (0ppm, 1 ppm, 2 ppm, and 3 pmm), each treatment repeated 5 times. The explant of the nodus were taken from plants produced by previous in vitro culture having 5 nodi. The basic medium used is New Phalaenopsis (NP) + Coconut Water + 1 ppm 2.4-D). The growth of axillary bud growth is measured based on the time of the bud emergence, growth of crown, and that of root. Data obtained were then analyzed using ANOVA. If there was a significant difference, the analysis was continued with a test of DMRT with a significant level of 5%. The results showed the addition of concentrations of 1 ppm and 2 ppm BAP influenced the time of the bud emergence, growth of crown, and that of root. The optimum concentration of BAP to induce the orchid axillary shoots is 2 ppm.

Antidiabetic Molecular Mechanisms of Active Compounds from Several Orchids Yulianti, Evy; Mercuriani, Ixora Sartika; Sugiyarto, Lili; Huang, Tzou-Chi
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.3940

Hyperglycemia condition that leads to diabetes causes various complications. Various active compounds from plants have been studied for their antidiabetic abilities. One of them is the orchid plant. Besides being used as decoration, orchids contain several active compounds that have been proven to be used in medicine, including diabetes. This article discusses the antidiabetic mechanism of several active compounds obtained from orchids. Publication regarding orchid plant for diabetes were found in databases such as PubMed, Google Scholar, Wiley, Science Direct, Medline, Scopus, and Springer. Keywords used in this study were â€œorchidâ€, â€œdiabetesâ€, â€œhyperglycemiaâ€, â€œcompoundâ€ and â€œherbalâ€. Out of the 447 collected articles (published in the period between 2011 and 2022), 416 were excluded due to non-relevant studies. There were 31 eligible studies included in this article. In conclusion, the antidiabetic mechanisms of the orchid extracts were as antioxidant, anti-inflammatory and anti-glycation agents, increasing insulin action, influencing lipid metabolism, and inhibiting Î±â€‘amylase and Î±â€‘glucosidase activity. Â

Analysis of Critical Thinking Ability of High School Students in Sleman Regency on Virus Material Sidabutar, Nurhalis; Mercuriani, Ixora Sartika
Jurnal Penelitian Pendidikan IPA Vol 10 No 3 (2024): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i3.5320

The benefits obtained if someone think critically is to make students active and effective in life, they can also develop understanding, evaluate different insights, develop problem solving skills so that later students have the ability to analyze further. The purpose of this analysis is to describe the critical thinking abilities of students in Sleman Regency. This research uses a quantitative descriptive method using an essay with 5 questions referring to indicators of critical thinking. Apart from that, the population in this analysis is all state high schools in Sleman Regency with a purposive random sampling technique of 9 schools, totaling 258 students. The results of the research show that students' critical thinking abilities are in the low category, so it is hoped that innovation will be needed in the form of developing methods, media, strategies and learning models that support students' 21st century abilities, especially in the aspect of critical thinking abilities.

Development of E-Module in Problem-Based Learning (PBL) on Biotechnology Material to Increase Learning Independence and Understanding of Concepts for Class XII SMA Students Dewi, Ratna; Mercuriani, Ixora Sartika
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8464

The aim of this study are determine the feasibility of developing an e-module in problem-based learning (PBL) with the flipped classroom learning method on biotechnology material to increase students' learning independence and understanding of concepts; determine the practicality of developing e-modules in problem-based learning (PBL) using the flipped classroom learning method for biotechnology material to increase students' learning independence and understanding of concepts; determine the effectiveness of e-module development in problem-based learning (PBL) using the flipped classroom learning method for biotechnology material to increase students' learning independence and understanding of concepts. The results of this research indicate that the e-module in problem-based learning (PBL) with the flipped classroom learning method is stated: very suitable for use based on the assessment of material experts with a value of 3.8 conversion 95 with a very feasible category and the assessment of media experts with a value 4.00 100 conversions with very decent category; very practical to use based on the results of the Biology Teacher's assessment with a score of 4.00 conversion 100 in the very feasible category and student assessment with a score of 3.87 conversion 97 in the very practical category; is effectively used to increase learning independence and conceptual understanding of phase F students based on the MANOVA test p < 0.05. The development of an e-module in problem-based learning (PBL) using the flipped classroom learning method on biotechnology material is feasible, practical and effective to use.

PENGARUH CAHAYA DAN TEMPERATUR TERHADAP PERTUMBUHAN TUNAS DAN PROFIL PROTEIN TANAMAN ANGGREK Phalaenopsis amabilis TRANSGENIK PEMBAWA GEN Ubipro::PaFT Putra, Rinaldi Rizal; Mercuriani, Ixora Sartika; Semiarti, Endang
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2483

Penelitian ini bertujuan untuk mencari kondisi yang tepat dalam percepatan pembungaan tanaman Phalaenopsis amabilis transgenik yang telah disisipi gen penentu waktu pembungaan Ubipro::PaFT. Metode penelitian ini menggunakan tanaman transgenik pembawa gen Ubipro::PaFT umur 18 bulan setelah penanaman. Tanaman ditumbuhkan pada inkubator dengan pencahayaan menggunakan lampu LED putih dan kombinasi LED putih biru, dengan fotoperiodisitas 8 jam terang 16 jam gelap, suhu 25ºC pada fase terang dan 20ºC pada fase gelap selama 20 minggu. Setelah 20 minggu pertumbuhan tanaman, dilakukan analisis profil protein dengan metode SDS-PAGE untuk mengetahui protein yang diproduksi pada setiap fase pertumbuhan yang diamati.Hasil penelitian menunjukkan kombinasi cahaya LED putih dan biru meningkatkan pembentukan daun sebesar 60%, panjang daun 70,58%, tetapi belum diperoleh kemunculan infloresen. Analisis profil protein menunjukkan terbentuknya protein dengan ukuran 108,57; 71,30; 56,16; 40,85; 26,79; 13,27; dan 13,12 kilodalton pada tanaman transgenik, tetapi tidak terdeteksi protein dengan ukuran 19,65 kDa yang sesuai dengan berat molekul protein PaFT, sementara protein dengan ukuran sekitar 56,16 kDa sesuai dengan berat molekul protein POH1(Phalaenopsis Orchid Homeobox1). Hal ini menunjukkan bahwa gen vegetatif POH1 mampu menghambat aktivasi gen PaFT pada tanaman P. amabilis transgenik umur 20 minggu, sehingga tanaman masih dalam fase juvenil dan belum mampu diinduksi untuk berbunga.

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