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All Journal Jurnal Penelitian Saintek Agrotrop : Journal on Agriculture Science Indonesian Journal of Biotechnology Jurnal Pendidikan Biologi Indonesia Bioeksperimen: Jurnal Penelitian Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Caraka Tani: Journal of Sustainable Agriculture Jurnal Bioedukatika Biosfer: Jurnal Pendidikan Biologi Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Jurnal Pertanian Agros AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Agroista: Jurnal Agroteknologi Kingdom (The Journal of Biological Studies) IJOBI
Ixora Sartika Mercuriani
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Neuroscience Physics Public Health

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PENGARUH CAHAYA DAN TEMPERATUR TERHADAP PERTUMBUHAN TUNAS DAN PROFIL PROTEIN TANAMAN ANGGREK Phalaenopsis amabilis TRANSGENIK PEMBAWA GEN Ubipro::PaFT Putra, Rinaldi Rizal; Mercuriani, Ixora Sartika; Semiarti, Endang
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2483

Abstract

Penelitian ini bertujuan untuk mencari kondisi yang tepat dalam percepatan pembungaan tanaman Phalaenopsis amabilis transgenik yang telah disisipi gen penentu waktu pembungaan Ubipro::PaFT. Metode penelitian ini menggunakan tanaman transgenik pembawa gen Ubipro::PaFT umur 18 bulan setelah penanaman. Tanaman ditumbuhkan pada inkubator dengan pencahayaan menggunakan lampu LED putih dan kombinasi LED putih biru, dengan fotoperiodisitas 8 jam terang 16 jam gelap, suhu 25ºC pada fase terang dan 20ºC pada fase gelap selama 20 minggu. Setelah 20 minggu pertumbuhan tanaman, dilakukan analisis profil protein dengan metode SDS-PAGE untuk mengetahui protein yang diproduksi pada setiap fase pertumbuhan yang diamati.Hasil penelitian menunjukkan kombinasi cahaya LED putih dan biru meningkatkan pembentukan daun sebesar 60%, panjang daun 70,58%, tetapi belum diperoleh kemunculan infloresen. Analisis profil protein menunjukkan terbentuknya protein dengan ukuran 108,57; 71,30; 56,16; 40,85; 26,79; 13,27; dan 13,12 kilodalton pada tanaman transgenik, tetapi tidak terdeteksi protein dengan ukuran 19,65 kDa yang sesuai dengan berat molekul protein PaFT, sementara protein dengan ukuran sekitar 56,16 kDa sesuai dengan berat molekul protein POH1(Phalaenopsis Orchid Homeobox1). Hal ini menunjukkan bahwa gen vegetatif POH1 mampu menghambat aktivasi gen PaFT pada tanaman P. amabilis transgenik umur 20 minggu, sehingga tanaman masih dalam fase juvenil dan belum mampu diinduksi untuk berbunga.
Development of an electronic student worksheet using local orchid potential to improve critical thinking and collaboration skills in biotechnology learning Annisa Ul Furqhoni; Ixora Sartika Mercuriani
JURNAL BIOEDUKATIKA Vol. 13 No. 3 (2025)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/bioedukatika.v13i3.31149

Abstract

This study aims to develop an electronic (e)-student worksheet  integrated with the Project-Based Learning (PjBL) model and to enhance the critical thinking and collaboration skills of Grade X high school students in biotechnology learning based on the local potential of the Nglanggeran Ancient Volcano Orchid. Employing a Research and Development (R and D) approach, the research used the ADDIE model consisting of Analysis, Design, Development, Implementation, and Evaluation in the product development process. The e-student worksheet was created digitally using Canva and included multimedia elements to increase student engagement. The study involved a limited trial with 15 students and a field trial with 70 students divided into experimental and control groups. Data collection methods included interviews, questionnaires, and pretest-posttest essay-based assessments. Instrument validation showed all items were valid and reliable, with a Cronbach’s Alpha score of 0.843. The results revealed moderate improvement in students’ critical thinking skills with an N gain of 0.67 and collaboration skills with an N gain of 0.61 in the experimental group. These findings indicate that the experimental group outperformed the control group, validating that the contextualized PjBL approach effectively facilitates deeper cognitive processing and reinforces interpersonal skills compared to conventional methods. Statistical analysis using MANOVA confirmed significant differences between the two groups with Wilks’ Lambda value of 0.006 and p value less than 0.001. Expert and user feedback indicated the product was practical, accessible, and contextually relevant.
Sustainable Environmental Education Through Local Potential Studies In The Gunungkidul Region Of Yogyakarta Miftakhul Khasanah; Ixora Sartika Mercuriani; Suyitno Aloysius
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12081

Abstract

Environmental education plays a crucial role in shaping the character of students as the next generation, enabling them to be aware and concerned about environmental sustainability. Through environmental education, students are equipped not only with theoretical knowledge but also with practical values and skills to encourage them to become agents of change. This study aims to examine the Gunungkidul region as a source of local potential learning information on the topic of environmental change in science or biology learning materials for students. To support its learning activities, this study also adopts environmental education learning trends that can be used as part of efforts to build sustainable environmental education. This research is a literature review, focusing on articles published between 2020 and 2025 to ensure the data used is current and relevant. The results of the study indicate that the Gunungkidul region has great potential as a source of information for science or biology learning for students. In terms of environmental education learning, there are approaches, models, teaching materials, and learning media that teachers can choose or combine to improve the effectiveness of environmental education learning, especially on the topic of environmental change, with the hope that there will be no more gaps in the student learning process, so that student learning outcomes can be more meaningful and sustainable to support environmental sustainability in the future.
Co-Authors Adinda Yuslia Rukmanandita Afrizal Haris Agus Slamet Akram Akram Anisa Maulidiya* Annisa Ul Furqhoni Aries Bagus Sasongko Astuti Astuti Astuti Ayu Purnamasari Aziz Purwantoro Bekti Sulistya Utami Bernadetta Octavia Candra, Puspa Mega Desi Indriani Dwi Kurniasari Eli Rohaeti ENDANG SEMIARTI Endang Semiarti Endik Deni Nugroho Evy Yulianti Fita Nilasari Heru Nurcahyo Heru Nurcahyo HESTIN YUSWANTI Huang, Tzou-Chi Isana Supiah Yosephine Louise Khoirunnisa Khoirunnisa Kun Sri Budiasih Lili Sugiarto Lili Sugiyarto Madhani, Anisa Tiara Miftakhul Khasanah Nur Fitriyana Nurfina Aznam Paramita Cahyaningrum Kuswandi Puspa Mega Candra Putra, Rinaldi Rizal Ratna Dewi Ratnawati Ratnawati Ratnawati Ratnawati Ratnawati Retno Arianingrum Rifqi, Daffa Muhammad Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Ririn Budiastuti Risma Wiharyanti Seonghoe Jang Septasari, Mistri Setiawan, Andri Dias Sidabutar, Nurhalis Sofiatul Milah Sukarti Moeljopawiro Sukarti Moeljopawiro Suyitno Aloysius Vlorensius Vlorensius Wika Rinawati Yuyun Fitriani