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All Journal Jurnal Penelitian Saintek Agrotrop : Journal on Agriculture Science Indonesian Journal of Biotechnology Jurnal Pendidikan Biologi Indonesia Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika Biosfer: Jurnal Pendidikan Biologi Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Jurnal Pertanian Agros AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Agroista: Jurnal Agroteknologi Kingdom (The Journal of Biological Studies) IJOBI
Ixora Sartika Mercuriani
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health

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Development of E-Module in Problem-Based Learning (PBL) on Biotechnology Material to Increase Learning Independence and Understanding of Concepts for Class XII SMA Students Ratna Dewi; Ixora Sartika Mercuriani
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8464

Abstract

The aim of this study are determine the feasibility of developing an e-module in problem-based learning (PBL) with the flipped classroom learning method on biotechnology material to increase students' learning independence and understanding of concepts; determine the practicality of developing e-modules in problem-based learning (PBL) using the flipped classroom learning method for biotechnology material to increase students' learning independence and understanding of concepts; determine the effectiveness of e-module development in problem-based learning (PBL) using the flipped classroom learning method for biotechnology material to increase students' learning independence and understanding of concepts. The results of this research indicate that the e-module in problem-based learning (PBL) with the flipped classroom learning method is stated: very suitable for use based on the assessment of material experts with a value of 3.8 conversion 95 with a very feasible category and the assessment of media experts with a value 4.00 100 conversions with very decent category; very practical to use based on the results of the Biology Teacher's assessment with a score of 4.00 conversion 100 in the very feasible category and student assessment with a score of 3.87 conversion 97 in the very practical category; is effectively used to increase learning independence and conceptual understanding of phase F students based on the MANOVA test p < 0.05. The development of an e-module in problem-based learning (PBL) using the flipped classroom learning method on biotechnology material is feasible, practical and effective to use.
The Potential of Red Algae Methanol Extract (Kappaphycus alvarezii) as an Antibacterial Agent Toward Pseudomonas aeruginosa and Enterococcus faecalis Bacteria Nabila Novitasari; Evy Yulianti; Ixora Sartika Mercuriani; Lili Sugiyarto; Nur Aeni Ariyanti
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.365-372

Abstract

Urinary tract infection (UTI) is a disease caused by both Gram-negative and Gram-positive bacteria. Red algae (Kappaphycus alvarezii) contain bioactive compounds derived from secondary metabolites with antibacterial properties. This study investigates the potential of Kappaphycus alvarezii methanol extract to inhibit the growth of Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 using the microdilution broth method, determining Minimum Inhibitory Concentration (MIC) values ranging from a concentration of 500 µg/mL to 1.9531 µg/mL. To elucidate the inhibition mechanism, nucleic acid, protein, and Ca2+ ion leakage assay were performed via spectrophotometry at MIC 1, MIC 2, and MIC 3 concentrations. Morphological changes in the bacterial cell membrane were further examined using Scanning Electron Microscopy (SEM) at MIC 2. The results demonstrated that, the methanol extract of K. alvarezii exhibited significant antibacterial activity against Enterococcus faecalis bacteria with an MIC value of 1.9531 µg/mL, but showed no inhibitory effect on Pseudomonas aeruginosa. The extract inducted membrane leakage, as indicated by a higher leakage value compared to negative controls and commercial antibiotics. In addition, the methanol extract of K. alvarezii caused alterations in bacterial cells. These findings highlight the potential of Kappaphycus alvarezii methanol extract as a promising antibacterial agent.
In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Risma Wiharyanti; Ixora Sartika Mercuriani; Evy Yulianti; Paramita Cahyaningrum Kuswandi
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
Analisis Kesiapan Laboratorium Kultur Jaringan Tingkat SMK dalam Pembelajaran dan Kewirausahaan Dumaris Taruli Artha Siahaan; Bebi Ramalia Alfi; Ixora Sartika Mercuriani; Rahmania Pamungkas
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.11395

Abstract

This research evaluates the readiness of the tissue culture laboratory at SMK Negeri 1 Pandak using an observation instrument of laboratory preparedness. This study focused on the laboratory infrasturucture and management system as the primary object of observation in seven aspects. The instrument's validation was conducted by two Expert of Tissue Culture experts to yield Aiken's V validity coefficients for the seven aspects of laboratory readiness observation instrument ranging from 0.585 to 0.661, indicating that the instrument is valid. Observations of the tissue culture laboratory's readiness also revealed an average total readiness percentage of 76.72%, with the infrastructure (84.61%), facilities (85.84%), and administration (85.71%) aspects categorized as "Very Ready". The occupational health and safety (70%), learning process support (80%), and entrepreneurship development (68.42%) aspects are in the "Ready" category. The laboratory dynamics aspect (62.5%) showed a "Less Ready" category, thus requiring special attention. This research confirms the validity of the instrument and provides an overview of the tissue culture laboratory's readiness, and it can be used to enhance the quality and effectiveness of tissue culture laboratories in vocational agriculture schools. In addition, this result contributes as a strategic evaluation model in the management of vocational education laboratories and provides concrete recommendations for the development of entrepreneurship-based science education through the optimization of facilities and standardized management systems
Co-Authors Adinda Yuslia Rukmanandita Afrizal Haris Agus Slamet Akram Akram Anisa Maulidiya* Annisa Ul Furqhoni Aries Bagus Sasongko Astuti Astuti Astuti Ayu Purnamasari Aziz Purwantoro Bebi Ramalia Alfi Bekti Sulistya Utami Bernadetta Octavia Candra, Puspa Mega Desi Indriani Dumaris Taruli Artha Siahaan Dwi Kurniasari Eli Rohaeti ENDANG SEMIARTI Endang Semiarti Endik Deni Nugroho Evy Yulianti Fita Nilasari Heru Nurcahyo Heru Nurcahyo HESTIN YUSWANTI Isana Supiah Yosephine Louise Khoirunnisa Khoirunnisa Kun Sri Budiasih Lili Sugiarto Lili Sugiyarto Madhani, Anisa Tiara Miftakhul Khasanah Nabila Novitasari Nur Aeni Ariyanti Nur Fitriyana Nurfina Aznam Nurhalis Sidabutar Paramita Cahyaningrum Kuswandi Puspa Mega Candra Putra, Rinaldi Rizal Rahmania Pamungkas Ratna Dewi Ratnawati Ratnawati Ratnawati Ratnawati Ratnawati Retno Arianingrum Rifqi, Daffa Muhammad Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Ririn Budiastuti Risma Wiharyanti Seonghoe Jang Septasari, Mistri Setiawan, Andri Dias Sofiatul Milah Sukarti Moeljopawiro Sukarti Moeljopawiro Suyitno Aloysius Tzou-Chi Huang Vlorensius Vlorensius Wika Rinawati Yuyun Fitriani