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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Jurnal Pendidikan Biologi Undiksha SIMBIOSIS BERITA BIOLOGI Jurnal Biodjati Eksakta : Berkala Ilmiah Bidang MIPA Agro Bali: Agricultural Journal International Journal of Applied Science and Sustainable Development (IJASSD)
ENIEK KRISWIYANTI
Program Studi Magister Ilmu Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana, Denpasar-Bali
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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Karakteristik Dan Viabilitas Serbuk Sari 38 Ragam Tanaman Kamboja (Plumeria spp.) Di Bali I Komang Alit Adi Sanjaya; Eniek Kriswiyanti; Anak Agung Ketut Darmadi
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p06

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik dan viabilitas serbuk sari ragam tanaman kamboja (Plumeria spp.) di Bali. Pengamatan karakteristik serbuk sari dengan menggunakan metode asetolisis, sedangkan pengamatan viabilitas serbuk sari dengan metode warna 1 % anilin blue. Pengukuran dilakukan mengunakan mikrometri. Hasil pengamatan terhadap 38 ragam tanaman kamboja menunjukkan adanya variasi panjang aksis polar antara 36,48 ± 4,91 – 57,87 ± 3,89 µm, diameter bidang ekuatorial antara 30,18 ± 4,64 – 55,24 ± 5,52 µm, indeks P/E antara 0,85 ± 0,10 – 1,42 ± 0,13 serta viabilitas serbuk sari sebesar 6,67 – 42,31%. Presentase viabilitas serbuk sari tertinggi dijumpai pada ragam P. alba L. 'Snow White' sebesar 42,31 sedangkan terendah dijumpai pada ragam P. rubra L. 'Red Ajanta'. Berdasarkan indeks P/E serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok subpheroidal hingga prolat. Berdasarkan panjang aksis polar serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok media hingga magna.
PENYELAMATAN EMBRIO Dendrobium anosmum Lindl. MELALUI KULTUR IN VITRO Putu Yuni Astriani Dewi; Ida Ayu Astarini; Eniek Kriswiyanti
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p10

Abstract

The aim of the research is to analyse germination percentage, effect of coconut water addition in the media to number of last stage embryos and development of final stage D. anosmum Lindl. embryo. Research was conducted at Plant Structure and Development of Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Udayana and UPT. Balai Benih Induk Tanaman Pangan dan Hortikultura Provinsi Bali from February 2015 to July 2015. Three type of media were employed, i.e. PDA, Organic, MS. Three different concentration of coconut was added to media, i.e. 0 ml/L 50 ml/L, 100 ml/L. Results showed that seed D. anosmum Lindl. on PDA, Organic and MS has not germinated yet on all media, only swollen cells observed. Seed dormancy and maturity of seed were the main factors affecting seed germination.  Addition of 100 ml / L coconut water in MS showed that media is capable to increase the formation of final stages embryos (> 32 cells). Embryo development on PDA and Organic largely showed cell stage embryos 1 to 8 cells, while MS media already showed embryo development stages of > 32 cells.
PEMANFAATAN TUMBUHAN PEKARANGAN SEBAGAI BAHAN OBAT ALTERNATIF DI DESA JIMBARAN, KECAMATAN KUTA SELATAN, KABUPATEN BADUNG, BALI Irawati Irawati; Eniek Kriswiyanti; A. A. Ketut Darmadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p10

Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis tumbuhan pekarangan yang dapat dimanfaatkan sebagai obat alternatif, bagian tumbuhan yang digunakan, cara pengolahan dan penggunaan serta untuk mengobati penyakit apa saja yang. Penelitian dilaksanakan pada Oktober 2016 sampai Januari 2017 di 13 banjar di Desa Jimbaran dan identifikasi tumbuhan di Laboratorium Sruktur Perkembangan Tumbuhan Program Studi Biologi FMIPA Universitas Udayana. Prosedur penelitian meliputi observasi lapangan, wawancara dengan metode purposive sampling dan snow ball dalam pemilihan responden, dokumentasi dan identifikasi. Data dianalisis secara deskriptif kualitatif. Dari hasil penelitian diperoleh 65 jenis (36 suku) dan suku yang paling banyak dimanfaatkan adalah Zingiberaceae. Bagian tumbuhan yang paling banyak digunakan adalah daun (47,1%), kemudian diikuti rimpang (12,9%), buah (11,4%), akar (7,1%), kulit batang (7,1%), bunga (4,3%), getah (2,9%), umbi (2,9%), biji (2,9%)dan akar gantung (1,4%). Cara pengolahan tumbuhan obat yang paling banyak adalah dengan cara direbus (44,8%), lalu diminum. Penyakit yang dapat diobati yaitu sebanyak 47 penyakit dalam dan 21 penyakit luar.
KEANEKARAGAMAN KARAKTER TANAMAN KELAPA (Cocos nucifera L. ) YANG DIGUNAKAN SEBAGAI BAHAN UPACARA PADUDUSAN AGUNG Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.477 KB)

Abstract

The study was aimed to identify the characteristic variations of the coconuts (Cocos nucifera L.) trees that are used as materials for the Padudusan Agung ceremony in Bali. Exploration, interview, and observation of character variations of coconuts have been conducted from coconut plantation area from each regency in Bali. The characteritations was identified based on measurements and observations of parts of plant according to “Discriptors for Coconuts”. The result shows that there was a narrow variation on the stem and leaf characters (2.81-11.83%), while high variations found on the fruit numbers in a bunche for the generative characters (42.8%). Padudusan Agung ceremony uses eleven types (cultivars) of coconuts. Those are: gadang, gading, bulan, and surya with green, yellow, white, red epicarpium colours respectively. Young fruit of Udang coconuts have red mesocarpium, sudamala has double spathas, bingin has root grown on stem, be julit has plicated primordium leaves, bojog has hush likes the color of monkey hairs, ancak has branched stem, and rangda has petiole twisted on the top of the stem.
INDEKS MITOSIS UJUNG AKAR KECAMBAH CABE BESAR (Capsicum annuum L.) SETELAH PERLAKUAN SUSPENSI Trichoderma sp. PetroneLa Deno Raja; Eniek Kriswiyanti; Ni Nyoman Darsini
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.708 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui indeks mitosis ujung akar kecambah cabe besar (Capsicum annuum L.) setelah perlakuan suspensi Trichoderma sp. Penelitian ini dilakukan di laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi FMIPA, Universitas Udayana dari Oktober 2013-November 2013. Metode yang digunakan adalah metode squash, biji cabe untuk kontrol direndam dalam air ± 6 jam, untuk perlakuan biji setelah direndam air, direndam lagi dalam suspensi Trichoderma sp. 10-7 selama ± 6 jam, selanjutnya dikecambahkan. Ujung akar kecambah 2 mm dipotong, difiksasi dalam larutan farmer ± 2-24 jam, dihidrolisis dalam larutan 3N HCL ± 2-5 menit dan kemudian pewarnaan dengan aceto orcein ± 5 menit. Pengamatan dilakukan dengan mikroskop binokuler, data pembelahan tiap fase mitosis dihitung (%), dicatat dan difoto, dan dianalisis dengan menggunakan uji paired T tes.Hasil penelitian menunjukkan bahwa Trichoderma sp. berpengaruh terhadap indeks mitosis sel ujung akar Capsicum annuum L.,  pada fase metafase berbeda nyata antara kontrol dan perlakuan, sedangkan pada fase profase, anafase dan telofase berbeda tidak nyata.  Pada perlakuan persentase fase profase, metafase, anafase dan telofase (77,14%; 12,96 %; 5,88 % dan 5,23 %) lebih tinggi dari kontrol (66,40 %; 5,44 %; 4,96 % dan 4,66 %).
THE GENERATIVE REPRODUCTIVE CHARACTERISTICS OF RED DRAGON FRUIT (Hylocereus.polyrhizus (Web.) Britton & Rose, CACTACEAE) Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.527 KB)

Abstract

This study aimed to investigate the generative reproductive characteristics of red dragon fruit (Hylocereus polyrhizus (Web.) Britton & Rose), that is the characteristics and the development of male and female reproductive system and the embryo. The flowering morphology was observed through the gametogenesis process and the process of embryogenesis was observed using the methods of squash, cytolysis, and embedding of flower before anthesis, during and after anthesis. The results showed that the flower funnelled form with many calyxes, stamens and crowns. The pollen was circular, sulcus, trilate, reticulate, and spheroid. The development of microgametophyte was at the 2nd stages with three nucleuses and the pistil developed after anthesis.  Anthesis was taken place at night time, fertilization and pollen developed before anthesis and has not germinating. A single pistil, the head of pistil with many branches, the style longer than the stamen, open type.  Seeds anathrophus, endosperm and embryo with the globular shaped, developed 5 days after anthesis, the embryo torpedo likes and the differentiation of primer tissues seen in the seeds 7 days after anthesis, and arilus generated from development of megasporangium. Keywords: embedding, self incompatibility, sferoidal, anatrophus, arillus.
KANDUNGAN FITOKIMIA EKSTRAK DAUN KAMBOJA (Plumeria sp.) DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN JAHE EMPRIT (Zingiber officinale var. Amarum) Putu Yayun Antari Budaya; Ni Putu Adriani Astiti; Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.468 KB)

Abstract

Tanaman kamboja mengandung senyawa alelopati yang menghambat pertumbuhan tanaman di sekitarnya.  Penelitian ini bertujuan untuk mengetahui kandungan fitokimia ekstrak daun kamboja dan pengaruhnya terhadap pertumbuhan tanaman jahe emprit (Zingiber officinale var. amarum). Penelitian dilaksanakan dalam dua tahap yaitu, tahap pertama uji fitokimia ekstrak daun kamboja merah dan daun kamboja putih, serta tahap kedua berupa uji respon pertumbuhan tanaman jahe emprit yang diberi perlakuan ekstrak daun kamboja. Variabel respon pertumbuhan yang diamati adalah jumlah tunas tumbuh, tinggi tanaman, jumlah daun, jumlah akar, berat rimpang, berat basah berangkasan, dan berat kering berangkasan. Hasil uji fitokimia menunjukkan bahwa pada ekstrak daun kamboja putih dan kamboja merah memiliki jenis golongan senyawa aktif yang sama yaitu triterpenoid, steroid, flavonoid, dan polifenol dalam intensitas yang berbeda. Ekstrak daun kamboja putih memberikan efekdaya hambat yang lebih kuat dibandingkan dengan ekstrak daun kamboja merah. Persentase daya hambat ekstrak daun kamboja putih terhadap tinggi tanaman jahe emprit sebesar 80,5%, berat basah berangkasan 64%, jumlah daun 54%, berat kering berangkasan 54%, jumlah tunas tumbuh 33,3%, dan berat rimpang 31,9%.
STUDI VARIASI UKURAN SERBUK SARI KEMBANG SEPATU (HIBISCUS ROSA-SINENSIS L.) DENGAN WARNA BUNGA BERBEDA NI MADE DENNI APRIANTY; ENIEK KRISWIYANTI
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The purpose of this study conducted to know variety of : long of axis polar, equatorial plane diameters, and P/E index of ten Kembang Sepatu (Hibiscus rosa-sinensis L.) with differnt colour flowers. Acetolysis method and 1 % Safranin staining used to make pollens slides, micrometri were used to measured long of polar axis and diameter of pollens. The result of this research showed : pollen structure have: 90,10 ± 3,02 ^im to 117,42 ± 1,37 l^m axis polar long, 89,66 ± 3,13 |^m to 112,92 ± 0,94 (xm equatorial plane diameter, and 0,99 to 1,04 P/E index. Based on these data is indicated that the type of the pollen are prolat sferoidal (except bud type red corolla, and white flower) polypantoporat type appertura and periporat ornamentation exine .
POLA REPRODUKSI PADA SALAK BALI (SA/ACCA ZA/ACCA VAR. AMBOINENSIS (BECC.) MOGEA ENIEK KRISWIYANTI; I KETUT MUKSIN; LUH WATINIASIH; MADE SUARTINI
Jurnal Biologi Udayana Vol 12 No 2 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Research on reproduction patterns of Balinese snake skin fruits (Salak Bali) has been conducted by identifying the availability of pollens on anthers of male and hermaphrodite flowers, the viability of pollens in the pistillum and the present of embryo(s) in the ovulum. Flower samples were collected from Sibetan and Muncan Villages, Karangasem regency. Sample preparations and identifications were conducted in "Stuktur dan Perkembangan Tumbuhan Laboratory" Department of Bilogy, FMIPA Udayana University. The results showed that the pollen has a single apperture, unisulcus, minuta, oblat sferoidal type. The length of polar axis was 15-24 jam (P) and equatorial diameter of 16-24 |im (E). Therefore, the P/E index was 0.99-1.0. The pollen viability before and after anthesis of hermaphrodite flowers was low (71.4% and 50.6% respectively), with very low pollen tube germinations (0.08% of before and 0.48% after anthesis). Similarly, on the anthers of male flowers, the viability of pollens before anthesis was low (75.7%) with pollen germination of 0.18%, and event lower in after anthesis flowers (28.8%) with 0.29% pollen tube germination. Squashed stigma before and after anthesis and 2 days after flowers were opened showed that there was no germination occurred. Therefore, there was no germination in the pistillum, but the embryo developed before flower anthesis, indicates that the reproduction pattern of Salak Bali occurred without fertilizations (agamospermy).
UJI VIABILITAS DAN PERKEMBANGAN SERBUK SARI BUAH NAGA PUTIH (HYLOCEREUS UNDATUS (HAW.) BRITTON & ROSE), MERAH (HYLOCEREUS POLYRHIZUS (WEB.) BRITTON & ROSE) DAN SUPER MERAH (HYLOCEREUS COSTARICENSIS (WEB.) BRITTON & ROSE) SETELAH PENYIMPANAN NI KADEK YUNITA SARI; ENIEK KRISWIYANTI; IDA AYU ASTARINI
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.582 KB)

Abstract

The aim of the research was to determine pollen viability, pollen tube length and pollen development of white, red and super red dragon fruit after storage at different temperatures and times. The method used to test pollen viability was hanging drop technique and to observe the development of pollen used acetolysis techniques. The results showed viability and pollen tube length of white, red and super red dragon fruit after storage at temperature of 10° C and -20° C for 1, 2 and 3 weeks decreased (66% and 25%), tended to increase (2% ) after 4 weeks. Viability and pollen tube length decreased (100%) after storage at 30° C for 4 weeks. Pollen development of white, red and super red dragon fruit after storage at 30°C, 10°C and -20°C for 1 to 4 weeks showed the majority of pollen consists of uninucleat and binucleat.
Co-Authors A.A.S. A. Sukmaningsih Alprida Yanti Anak Agung Istri Ratih Gayatri Anak Agung Ketut Darmadi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Eka Budi Mursafitri Gusti Ayu Nyoman Budiwati Gusti Ayu Nyoman Budiwati Hanas, Dicky Frengky I Gusti Ayu Sugi Wahyuni I Ketut Junitha I KETUT MUKSIN I Komang Alit Adi Sanjaya I Made Saka Wijaya I NYOMAN RAI I Putu Sudiartawan I Wayan Sumerta Yasa I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Made Bramasta Wira bumi Ida Bagus Made Bramasta Wirabumi Ida Bagus Wayan Gunam Irawati Irawati Junita Hardini Krisdayani, Putu Mita Luh Putu Melandani Made Ria Defiani Meitini Proborini Wahyuni Muhammad Ibadullah Ni Kadek Sari Dewi Handayani NI KADEK YUNITA SARI Ni Luh Gede Cerli Tonika Sari Ni Luh Putu Ariwathi Ni Luh Putu Rika Sugiantari Ni Luh Suriani Ni Luh Watiniasih NI MADE DENNI APRIANTY Ni Made Dwi Sri Wulandari Ni Made Puspawati Ni Made Suartini NI NYOMAN DARSINI Ni Putu Adriani Astiti Ni Putu Ariwathi Ni Putu Muni Raisani Ni Putu Sri Risa Dewi Ni Putu Yuni Astriani Dewi Ni Wayan Ayu Riantini Pande Ketut Sutara PetroneLa Deno Raja Pramesti, Ni Kadek Rika Putu Suwardike Putu Yayun Antari Budaya Rindang Dwiyani Sista Nirmala Wenni Setyo Lestari, Wenni Setyo Wijaya, I Made Saka Wira bumi, Ida Bagus Made Bramasta Zaenul Gafari