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Journal : AGROMIX

Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu
AGROMIX Vol 16 No 1 (2025)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Co-Authors - Hamid Agung, I Dewa Agung Putra AGUS PUTRA SEDANA Ali Nurmasyah Americo Alves Brito Anak Agung Ayu Agung Sri Sunari Anak Agung Sagung Kendran ANANDA RIZKI NURANI ARIYANTA, I PUTU BAWA Chiharu Hongo Damastra, Garda Bagus Desak Putu Sudarmini Devi, Komang Saraswati Devi, Putu Shinta DEWA AYU DIAH PUSPITASARI Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DINA RAHMAWATI Dinarkaya, Shah Mahapati Dwi Martiningsia Dwi Martiningsia DWI SUGIARTA DWI WIDANINGSIH Eka Wijayanti, Febri G.A.M.K. Dewi GARGITA, I WAYAN DIKSA Gede Suastika Gede Suastika GREG LUTHER GREGORY C. LUTHER Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Alit Susanta Wirya GUSTI NGURAH GEDE DHARMA PUTRA PUTRA Hamid, - Harianja, Shara Yulita Hartha , I Komang Gede Suweca I DEWA MADE PUTRA WIRATAMA I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I G. Wijana I GEDE PUTU WIRAWAN I GEDE SILA ADNYANA I Gst Agung Putu Raka Agung I GUSTI AYU DEVI VALENIA SARI I Gusti Ngurah Bagus I Gusti Ngurah Kade Mahardika I Ketut Mangku Budiasa I KETUT PURNA YASA I KETUT SUMIARTHA I Komang Candra Giri Prayoga I KOMANG VIDIA DHARMA TARO PUTRA I Made Arimbawa I Made Dira Swantara I Made Sudana I MADE SUDARMA I Made Sukewijaya I MADE SUPARTHA UTAMA I MADE WINANTARA I MADE YESTA SANTIATMA I NYOMAN DARMA YASA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I PUTU DHARMA I PUTU WIRYA SUPUTRA I Wayan Arta Wijaya I WAYAN RUSMAN I Wayan Suardana I Wayan Supartha I Wayan Susila I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari IDA BAGUS GDE PRANATAYANA Ida Bagus Kade Suardana JOKO MARIYONO JOKO MARIYONO KETUT AYU YULIADHI Klett, Katrina Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Mika Mega Astuthi Made Satya Andrayuga MARIA ULFA ELLA ELLA Masahiro Shishido MOCH BINTANG RAMADHAN NI KADEK NINA ARI SUCI Ni Kadek Nita Karlina Astriyani Ni Luh Gede Sudaryati NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE DWI ANTARI Ni Made Dwi Desiyanti Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE WINDA UTARI NI NENGAH DARMIATI Ni Putu Merthaningsih Ni Putu Noviyanti Ni Wayan Korniasih NI WAYAN SUNITI NYOMAN SEMADI ANTARA Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna R. SRINIVASAN RAMADHAN, MOCH BINTANG S.K. Widyastuti SANJAYA, I GUSTI NGURAH PRABU WIRA Selangga, Dewa Gede Wiryangga SONIA ASHA HASARI Sri Kayati Widyastuti SUGIARTA, DWI Sumartayasa, I Wayan Andi SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Yuliadhi , Ketut Ayu Zhao, Tiejun