Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : HUMANIORASAINS

AKTIVITAS ANTIBAKTERI ACTINOMYCETES DARI AKAR TANAMAN DI EKOSISTEM KARST GORONTALO, INDONESIA Alfandi Kibu; Yuliana Retnowati; Novri Youla Kandowangko; Wirnangsi Din Uno; Abubakar Sidik Katili; Nurdin
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several pathogenic bacteria such as Staphylococcus aureus, Escherichia, Pseudomonas aeruginosa and other pathogenic bacteria have developed resistance to many marketed antibiotics. Staphylococcus aureus is already resistant to several types of antibiotics such as penicillin, erythromycin, cefoxitin, levofloxacin, and clindamycin, Escherichia is resistant to several antibiotics such as Penicillin G, Cefotaxime, Tetracycline, Ampicillin, and Amoxicillin, Pseudomonas aeruginosa has been resistant to antibiotics that target ribosomes, such as tobramycin and tigecycline. The problem of resistance of several pathogenic bacteria can be overcome by finding new metabolite compounds from Actinomycetes microorganisms that have antibiotic potential by exploring extreme areas, one of which is in the karst area, especially in plant roots. Root sampling was carried out by taking samples + 20 cm on each type of plant found. Isolation of Actinomycetes bacteria was carried out using the plate method. Antibacterial activity testing was carried out using the line method. The morphological form of the spores was seen using a SEM (Scanning Electron Microscope). The results of the study found 2 isolates of isolated plant roots containing Actinomycetes bacteria. 2 isolates showed morphological characteristics of mycelium, oval and round in shape and had various colors. The results of the antibacterial activity test showed that each isolate could inhibit several types of pathogenic bacteria, seen from the clear zone with different diameters and the results of SEM (Scanning Electron Microscopy) showed that 2 isolates had the same character and shape of spores as Actinomycetes, namely the genus Streptomyces.
STRUKTUR VEGETASI MANGROVE DI DESA POPALO KABUPATEN GORANTALO UTARA Nurlena Duhe; Marini Susanti Hamidun; Abubakar Sidik Katili; Febriyanti; 5Ilyas H. Husain; Waode Faridawaty
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study focuses on observing the structure of mangrove vegetation in Popalo Village, North Gorontalo Regency. Scientists found that the condition of mangrove forests in the area is not uniform, with some areas still well maintained, especially near rivers and beaches, while other areas, especially near settlements, have been damaged due to land conversion into ponds. This type of research is descriptive quantitative using a survey method. The most dominant type of mangrove found is Rhizophora. In addition, this study also shows that environmental factors such as temperature, air acidity, and salt levels greatly affect the growth and development of mangroves. Overall, the results of this study highlight the importance of better conservation and management efforts for mangrove forests in Popalo Village to maintain the sustainability of coastal ecosystems.
POTENSI ACTINOMYCETES DARI RHIZOSFER TUMBUHAN DI EKOSISTEM KARST GORONTALO SEBAGAI ANTICANDIDA Riskanarti K. Lihaawa; Yuliana Retnowati; Abubakar Sidik Katili
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Karst ecosystem is one of the extreme marginal areas or types of soil that has low soil fertility. Soil in the karst ecosystem has a relatively high calcium (Ca) content which can affect the availability of phosphorus (P). However, there is microbial activity that can provide nutrient needs for plants to stay alive, one of which is Actinomycetes. This research method uses a quantitative research method, rhizosphere soil samples were taken at three locations, namely Bangga hill, Panipi hill around Lake Limboto, and Olohuta hill with a purposive sampling method. The results of the study showed that there were 7 isolates of Actinomycetes found associated with 5 types of plants in the karst ecosystem. Actinomycetes isolates showed almost uniform morphological, colony and cell characteristics dominated by white. The results of the anticandida activity test showed that there were 2 isolates (KSLl and KSIc) that inhibited the growth of Candida albicans, KSLl with an inhibition zone diameter of 10.5 mm and KSIc with an inhibition zone diameter of 17.5 mm. Molecular identification based on 16S RRNA gene sequences showed that the KSLl isolate was closely related to Streptomyces aegyptia with a similarity index of 99.64% and the KSIc isolate was closely related to Istreptomyces sp with a similarity index of 99.57%.