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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture Syntax Literate: Jurnal Ilmiah Indonesia FENOMENA Jurnal Riset Biologi dan Aplikasinya Journal of Multidisciplinary Applied Natural Science Makara Journal of Science Sewagati Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Anjar Tri Wibowo
1Department Of Biology, Faculty Of Science And Technology, Airlangga University, Surabaya, Indonesia. 2Biotechnology Of Tropical Medicinal Plants Research Group, Faculty Of Science And Technology, Airlangga University, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Produksi Cabai Melalui Teknik Hidroponik Irigasi Tetes untuk Meningkatkan Pendapatan Komunitas Pemuda Tentena, Kabupaten Poso, Sulawesi Tengah Manuhara, Yosephine Sri Wulan; Wibowo, Anjar Tri; Sugiharto, Sugiharto; Sugiarso, Djarot
Sewagati Vol 9 No 3 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i3.2322

Abstract

Kota Tentena adalah salah satu pusat wisata dengan adanya danau Poso yang sangat terkenal. Namun masih terdapat  masyarakat yang hidup miskin. Kelompok pemuda Pukat Tentena di desa Tentena, Kabupaten Poso merupakan kelompok yang mengarah pada ekonomi produktif yang dalam beberapa tahun ini memiliki usaha pertanian sayur dan buah secara hidroponik untuk menampung pemuda dari daerah pelosok yang ingin melanjutkan sekolah namun tidak memiliki biaya. Namun masih banyak pemuda yang belum dapat dibantu untuk membiayai sekolah mereka. Sementara itu Komunitas Pemuda masih memiliki lahan yang tidak produktif. Oleh karena itu melalui program Kemitraan Masyarakat ini dilakukan kegiatan untuk memanfaatkan lahan tidak produktif tersebut sebagai lahan produksi cabai melalui teknik hidroponik irigasi tetes. Metode pelaksanaan dilakukan dalam beberapa tahap yaitu pembuatan rumah kaca (green house), pelatihan budidaya cabai menggunakan teknik hidroponik sistem irigasi tetes, penanaman benih cabai, pembuatan hidroponik sistem irigasi tetes, pemindahan dan pemeliharaan bibit cabai dalam sistem irigasi tetes.Setelah dipelihara selama lebih kurang 60 hari tanaman mulai berbunga dan berbuah. Untuk pemanenan cabai bisa dilakukan beberapa kali. Pada panen perdana ini diperoleh lebih kurang 250 g per tanaman. Hasil penjualan cabai dapat menambah pendapatan pemuda Tentena sebagai sasaran Mitra Pengabdian.
Expression of the curculin gene from different organs of Curculigo latifolia under different culture conditions Muslihatin, Wirdhatul; Fajar, Nur Syamsiatul; Wibowo, Anjar Tri; Manuhara, Yosephine Sri Wulan
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1131

Abstract

Curculigo latifolia is the main source of curculin. Curculin is a special kind of protein that tastes sweet and can change the way food tastes, and is naturally expressed in fruit. This study aims to measure and compare the relative expression of the curculin gene in different organs, i.e., fruit and leaf, under in vitro and in vivo conditions. In this study, mRNA isolation was carried out in tissues derived from fruits and leaves grown in vitro and in vivo (in the soil) of C. latifolia from West Java, Indonesia. Leaves from 20 weeks seedling on polybag, fruit 40 days after anthesis, and leaves from 20 weeks seedling of C. latifolia cultured on MS0 medium that were used in this experiment. The relative expression was measured using qRT-PCR. The results showed that the comparison of curculigo’s parts in the leaves was lower than in the fruit. The lowest expression was obtained in leaves grown under in vitro conditions at 0.001-fold, while leaves grown in the soil at 0.566-fold compared to curculin gene expression in fruit. This study concludes that the expression of the Curculin gene in fruit is different between the fruit and leaves, and the growth conditions have an influence on Curculin gene expression, where Curculin gene expression under in vitro conditions is lower than expression under in vivo conditions. The curculin is not only expressed in the fruit but also the leaves, so it has the potential to be developed.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Induksi Kalus Amorphophallus titanum (Becc.) Becc. Melalui Kultur In Vitro: Callus Induction of Amorphophallus titanum (Becc.) Becc. Through In Vitro Culture Dannis Yuda Kusuma; Anjar Tri Wibowo; Yosephine Sri Wulan Manuhara
Jurnal Hortikultura Indonesia (JHI) Vol. 15 No. 3 (2024): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.15.3.133-139

Abstract

Bunga bangkai raksasa (Amorphophallus titanum (Becc.) Becc. merupakan jenis tumbuhan yang dilindungi. Regenerasi biji di habitat alami sulit dan memerlukan waktu yang lama. Konservasi ex situ dengan kultur in vitro menjadi alternatif pemulihan populasi di habitat alaminya. Tujuan penelitian ini adalah mengetahui jenis eksplan dan zat pengatur tumbuh terbaik untuk menginduksi kalus A. titanum. Jenis eksplan yang digunakan antara lain petiolus, percabangan lamina daun dan lamina daun, sedangkan zat pengatur tumbuh yang digunakan antara lain BAP, CPPU, dan TDZ. Hasil penelitian menunjukkan petiolus yang dikultur secara vertikal mampu menghasilkan kalus lebih banyak dan waktu yang lebih pendek dibandingkan yang dikultur secara horisontal. Eksplan petiolus yang dikultur secara vertikal merupakan eksplan yang menghasilkan kalus terbanyak dan waktu inisiasi tercepat dibandingkan eksplan percabangan lamina daun dan lamina daun. Media MS dengan penambahan TDZ 1 mg L-1 merupakan media terbaik dalam menghasilkan kalus. Waktu inisiasi kalus tercepat diperoleh pada media MS dengan penambahan TDZ 1 mg L-1 dengan eksplan petiolus yang dikultur secara vertikal. Kata kunci: Amorphophallus titanum, petiolus, thidiazuron, zat pengatur tumbuh
The Effect of Lead (Pb) Content on the Number of Stomata on Tabebuia aurea Leaves in Surabaya City Hamidah, Hamidah; Chrisetioningsih, Nadia Immanuela; Wibowo, Anjar Tri
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i11.54956

Abstract

This study aimed to investigate the impact of lead (Pb) content on stomatal density in Tabebuia aurea leaves across various locations in Surabaya. Samples were collected from three distinct areas with different traffic densities: Campus C of Universitas Airlangga, Menur Road, and Prapen Road. Lead (Pb) content was analyzed using atomic absorption spectroscopy (AAS). The results revealed that the average lead (Pb) content absorbed by Tabebuia aurea leaves due to vehicle emissions was 1.06 mg/kg at Campus C, 1.78 mg/kg at Menur Road, and 2.35 mg/kg at Prapen Road. Stomatal density in Tabebuia aurea leaves was found to be 119.25 ± 3.605 stomata/mm² at Campus C, 123.88 ± 1.000 stomata/mm² at Menur Road, and 140.92 ± 7.211 stomata/mm² at Prapen Road. These findings suggest that stomatal density in Tabebuia aurea leaves is lower on Menur Road compared to Prapen Road but higher than at Campus C. This indicates a positive correlation between stomatal density and lead (Pb) content in Tabebuia aurea leaves.
Optimization of Plant Growth Regulators and Bioreactor Systems for Efficient In Vitro Shoot Multiplication and Elongation of Amorphophallus muelleri Blume Jayanti, Nur Afni; Putri, Angelia Krisdanti Nasera; Manuhara, Yosephine Sri Wulan; Wibowo, Anjar Tri
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 4 (2025): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i4.103957

Abstract

Porang (Amorphophallus muelleri Blume), an Indonesian endemic species rich in glucomannan, is widely utilized in food industries for its health benefits. However, its traditional vegetative propagation through bulbils produces limited plants, necessitating an efficient in vitro multiplication system. This study aimed to optimize plant growth regulator combinations and bioreactor systems for enhanced shoot induction and elongation of A. muelleri. During the initiation and induction stages, combinations of thidiazuron (TDZ) with benzylaminopurine (BAP) or kinetin at concentrations ranging from 0 to 1.00 mg l⁻¹ were applied to solid MS media and observed for 8 weeks. The combination of TDZ 0.50 mg l⁻¹ + BAP 1.00 mg l⁻¹ produced the fastest shoot induction (32.34±2.52 days), while TDZ 0.75 mg l⁻¹ + BAP 0.75 mg l⁻¹ resulted in the highest shoot number (76.33±22.81 shoots explant⁻¹) and 100% shoot formation. TDZ combined with kinetin had no significant effect on induction parameters. For elongation, shoots induced from TDZ 0.75 mg l⁻¹ + BAP 0.75 mg l⁻¹ medium were cultured in two bioreactor systems: a Temporary Immersion System (TIS) and a Balloon Type Bubble Bioreactor (BTBB). The BTBB significantly outperformed the TIS, yielding 85.2±3.35 shoots explant⁻¹ with an average shoot length of 5.67±1.13 mm after 4 weeks. These results indicate that the synergistic use of TDZ and BAP, combined with the BTBB system, substantially improves in vitro shoot multiplication and elongation of porang, providing a reliable approach for its large-scale propagation and conservation.
Co-Authors Abdul Rahman Siregar, Abdul Rahman Abywijaya, Ilham Kurnia Agus Supriyanto Alfinda Novi Kristanti Almando Geraldi Awik Puji Dyah Nurhayati Ayu Lestari, Dewi Boon Chin Tan Boon Chin Tan BUDI SETIADI DARYONO Cahyo Wulandari Chrisetioningsih, Nadia Immanuela Dannis Yuda Kusuma Dannis Yuda Kusuma Djarot Sugiarso Dwi Kusuma Wahyuni Dwi Winarni Felayati, Themas Firli Rahmah Primula Dewi Hamidah . Himawan, Tyas Ikhsan Idqa Nurtri Bhakti Intan Ayu Pratiwi, Intan Ayu Jayanti, Nur Afni Jihans, Fira Fitria Junairiah Junairiah Kusuma, Dannis Yuda Latifah, Vida Rahma Listijani Suhargo, Listijani Manikya Pramudya Meitha, Karlia Miftahudin . Muhammad Hilman Fu'adil Amin Nabilatun Nisa Novitasari, Yeyen Nugrahapraja, Husna Nugroho, Giri Nur Syamsiatul Fajar, Nur Syamsiatul Priyono, Dwi Sendi Putri, Angelia Krisdanti Nasera Putri, Wahyu Aristyaning Putro, Karso Suryo Rachman, Mifta Pratiwi Ridesti Rindyastuti S. Sugiharto Sahat Saragih Sartika, Dian Sebastian, Alfino Sofyana, Neng Tanty Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Subiastuti, Aprilia Sufi Sugiharto TATIK CHIKMAWATI Tuty Arisuryanti Ufairanisa Islamatasya Wijayanti, Ely Tri Yekti Asih Purwestri Yosephine Sri Wulan Manuhara Yusuf, Adib Fakhruddin