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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung AL KAUNIYAH e-Journal Pustaka Kesehatan Jurnal Sains dan Teknologi Althea Medical Journal Jurnal Kesehatan Andalas Research Journal of Life Science Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Health Notions Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Pengabdian Pada Masyarakat Warta Pengabdian Multidiciplinary Journal Al-sihah: The Public Health Science Journal Manuju : Malahayati Nursing Journal Majalah Kedokteran Andalas Jurnal Inovasi Teknik Kimia AloHA International Journal of Health Advancement (AIJHA) Jurnal Kesehatan dr. Soebandi Strada Jurnal Ilmiah Kesehatan (SJIK)
Erma Sulistyaningsih
Department Of Parasitology, Faculty Of Medicine, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Journal : UNEJ e-Proceeding

 1 
Specific sequence of Plasmodium falciparum DBL domains associated with severe malaria outcome Sulistyaningsih, Erma; Fitri, Loeki Enggar; Loescher, Thomas; Berens-Riha, Nicole
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Duffy-binding like (DBL) domains of Plasmodium falciparum are believed to be involved in erythrocytes invasion and infected erythrocytes cytoadhesion during the blood stage of malaria infection. In Plasmodium falciparum, DBL domains found in the two different protein families; Erythrocyte Binding Ligand (EBL) including EBA-175, EBA-140, EBA-181 and EBL-1, and Plasmodium falciparum Erythrocyte Membrane Protein-1. The study aimed at investigating the specific sequence of Plasmodium falciparum involved in severe malaria outcome.Blood samples from severe and uncomplicated falciparum malaria cases from Papua and South Kalimantan province, Indonesia, were collected for DNA extraction. A dried blood on filter paper were used for RNA extraction. PCR was performed using UNIEBP primers and directly sequenced. Internal var D primers were designed according to the sequencing of the ~550 bp band produced by UNIEBP primers. The nucleotide sequences were analyzed by NCBI BLAST. Multiple bands ranging from nearly 250 bp to 1 kb were resulted from gDNA in all samples. Two isolates yielded bands of 450 and 525 bp, three isolates showed three bands additionally 250 bp, one isolate presented four bands additionally 800 bp and one isolate resulted one band additionally 1 kb.Amplification of cDNA from severe malaria cases produced one to four bands ranging from 250 bp to 700 bp, and no band observed from cDNA of uncomplicated malaria. Sequencing of the 418 bp bands matched with the eba-175 gene, the 316 bp determined as DBL1a domain and 486 bp band matched with the DBLg domain isolated from placenta of PAM’s Malawian woman. The expression of a 237 bp sequence corresponding to var D gene, was detected solely in severe malaria patients, implicating an association of gene expression and manifestation of severe malaria. Further characterization of the var D gene with a larger sample size is required to draw a definite conclusion. Keywords: DBL domains, Plasmodium falciparum, severe malaria, var D gene.
Specific sequence of Plasmodium falciparum DBL domains associated with severe malaria outcome Erma Sulistyaningsih; Loeki Enggar Fitri; Thomas Loescher; Nicole Berens-Riha
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Duffy-binding like (DBL) domains of Plasmodium falciparum are believed to be involved in erythrocytes invasion and infected erythrocytes cytoadhesion during the blood stage of malaria infection. In Plasmodium falciparum, DBL domains found in the two different protein families; Erythrocyte Binding Ligand (EBL) including EBA-175, EBA-140, EBA-181 and EBL-1, and Plasmodium falciparum Erythrocyte Membrane Protein-1. The study aimed at investigating the specific sequence of Plasmodium falciparum involved in severe malaria outcome.Blood samples from severe and uncomplicated falciparum malaria cases from Papua and South Kalimantan province, Indonesia, were collected for DNA extraction. A dried blood on filter paper were used for RNA extraction. PCR was performed using UNIEBP primers and directly sequenced. Internal var D primers were designed according to the sequencing of the ~550 bp band produced by UNIEBP primers. The nucleotide sequences were analyzed by NCBI BLAST. Multiple bands ranging from nearly 250 bp to 1 kb were resulted from gDNA in all samples. Two isolates yielded bands of 450 and 525 bp, three isolates showed three bands additionally 250 bp, one isolate presented four bands additionally 800 bp and one isolate resulted one band additionally 1 kb.Amplification of cDNA from severe malaria cases produced one to four bands ranging from 250 bp to 700 bp, and no band observed from cDNA of uncomplicated malaria. Sequencing of the 418 bp bands matched with the eba-175 gene, the 316 bp determined as DBL1a domain and 486 bp band matched with the DBLg domain isolated from placenta of PAM’s Malawian woman. The expression of a 237 bp sequence corresponding to var D gene, was detected solely in severe malaria patients, implicating an association of gene expression and manifestation of severe malaria. Further characterization of the var D gene with a larger sample size is required to draw a definite conclusion. Keywords: DBL domains, Plasmodium falciparum, severe malaria, var D gene.
Co-Authors Agustina Tri Endharti Al Munawir Alami, Eqiel Navadz Akthar Aldi Cahya Muhammad Alfi Kamalia Aliyatul Farida Anas Bakhtiar Diyansah Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Aprilia, Annisa Nadya Ardyansyah, Rizky Robeth Arifin, Mochammad Samsul Ayundasari, Asti Bagus Hermansyah Burhan, Niniek Cholis Abrori, Cholis Cicih Komariah Denaneer Rahmadatu Devita Prima Nurmasari Dewantoro, Dicky Didi Candradikusuma Didi Candradikusuma Dini Agustina Dion Krismashogi Darmawan Dwi Wahyuni Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Endang Sri Purwanti Wulandari Erfan Efendi Eva Sulawati Arum Fathul Hidayatul Hasanah, Fathul Hidayatul Febrina Sylva Fridayanti, Febrina Sylva Firdha Novitasari Fitri Dika Maharani FX Ady Soesetijo, FX Ady Ghani Silahudin Hadi Prayitno Heni Fatmawati Hidayat Sujuti I Dewa Ayu Ratna Dewanti I Ketut Suada Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Intan Rachmawati Irawan Fajar Kusuma Istinaroh, Nurul Iza Billa Fahmi Jarot Subandono Jarot Subandono Jauhar Firdaus Josef Sem Berth Tuda Kahfi Karunia Ilahi Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Laeli Kurniasari Laili, Elisa Fadia Laily Rahmawati Loeki Enggar Fitri Luh Putu Ratna Sundari Muhammad Hasan Ngurah Agung Reza Satria Nugraha Putra Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Burhan Nur Aqmarina Kusumawardani Nurmaida, Eny Pralampita, Pulong Wijang Pungky Setya Arini Putri, Izza Amalia Rahma, Nafisah Hani Asyifah Rina Fitriana Rina Fitriana Ristya Widi Endah Yani Rosita Dewi Salim Salim Samsul Arifin Sari, Leny Yulia Widia Sa’diyah, Nindya Audatus Setyoadji, Winnie Almira Sheilla Rachmania, Sheilla Sillehu, Sahrir Silvi Ahmada Chasya Sinta Ariyani Sri Hernawati Sugiyanta Sugiyanta Sukarti Moeljopawiro Sukarti Moeljopawiro Thomas Loescher Thomas Loescher, Thomas Tri Agus Siswoyo Tristira Urvina Tuda, Josef Sem Berth Umar Zein Warda Ayu Nadira Wayan T. Artama Wayan T. Artama Widhi Dyah Sawitri Wiji Utami Wiwien Sugih Utami Yunita Armiyanti Zahniar, Zahniar