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Journal : Biocelebes

Metode Inokulasi dan Pengamatan Perkembangan Phytophthora palmivora Serta Gejalanya Sebagai Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) Dian Sri Anugrah; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Phytopthora palmivora is one of pathogenic mushroom causes black pod   iisease of cocoa plant. The research about P. palmivora development and symptoms as the cause black pod   disease of cocoa (Theobroma cacao L.) was conducted from March until August 2016. Inoculation treatment of P. palmivora compared with aquades qontrols carried on cocoa fruit, with use “singlespot” and “polyspot” method. Observation parameters include incubation periode, extensive spotting, the percentage of P. palmivora infection and observation of macroscopic and microscopic P. palmivora mushroom. The results showed that in P. palmivora infection attack on cocoa fruit tends to grow up from third day to seventh day with average extensive spotting infection start on third day to seventh day are 6,4 cm, 8,1 cm, 9,6 cm, 12,72 cm, and 12,20 cm. Extensive spotting average P. palmivora infection most big is 14,22 cm while the percentage average most big is 90% and macroscopic structure showing their blackish brown spot on fruit surface and derived morphological characters of the hyphae are not insulated and ramified. Visible mycelium, clamydospores, papilla, zoospore dan sporangium forms such as Pier fruit on microscopic observation.
PERTUMBUHAN MISELIUM Trichoderma sp. PADA LIMBAH CAIR TEMPE DAN LIMBAH AIR KELAPA Juliana Juliana; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The purpose study was to determine potential tempe industry liquid waste and coconut water waste as medium propagation miselium Trichoderma sp. The treatment was conducted based on completely randomized design, consisting of seven treatments and three replication, namely: P0 (control), P1 (tempe industry liquid waste 100%), P2 (tempe industry liquid waste 80% + coconut water waste 20%), P3 (tempe industry liquid waste 60% + coconut water waste 40% ), P4 (tempe industry liquid waste 40% + 60% coconut water waste), P5 (tempe industry liquid waste 20% + 80% coconut water waste), P6 (100% coconut water waste). Variable observation included are media pH formula and the weight of mycelium biomass Trichoderma sp. The results showed that Trichoderma sp. Can be grown on all treatments except treatments P0 (control). The treatments produced in the highest mycelium biomass is P3 : 1595,333 mg per 100 mL medium, and this not significantly different from P2 : 1566,667 mg, but significantly different with all treatments.
Pengamatan Oncobasidium theobromae secara Makroskopis dan Mikroskopis, serta Gejala Serangan Sebagai penyebab Penyakit Vascular Streak Dieback (VSD) pada Tanaman Kakao di Kabupaten Sigi, Sulawesi Tengah Sitti Ardianti; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.543 KB)

Abstract

Oncobasidium theobromae are fungi that causes disease Vascular Streak Dieback (VSD) on the cocoa plants which can causes a totaly death, because the mechanism attack which systemic. The purpose of this study was to observe the morphology of O. theobromae macroscopically and microscopically, as well as symptoms of VSD attack on the cacao plants. This research was conducted from March to August 2016 in the Laboratory of Biotechnology Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University (FMIPA UNTAD). The result of macroscopic observation showed that O. theobromae have white mycelium on the early growth, then the color change to cream and texture like cotton. In microscopic observation, it has branched mycelium and irregular septate. VSD attack symptoms characterized with the leaf has necrosis, the former sitting leaf and the leaf base has three dots, twigs become  toothless and the ends of twigs become dry.