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Journal : Makara Journal of Science

Sequence Analysis of the Cytochrome C Oxidase Subunit I Gene of Pseudagrion pilidorsum (Odonata: Coenagrionidae) Tallei, Trina Ekawati; Koneri, Roni; Kolondam, Beivy Jonathan
Makara Journal of Science Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

Pseudagrion pilidorsumis1of over 140 species of Pseudagrion (in thefamily Coenagrionidae), the largest genus of damselfly. This species exhibits dimorphism due to the different body colorations of males and females, making them difficult to distinguish from other congeneric species. This study analyzed thecytochrome C oxidase subunit I (COI) gene sequence of P.pilidorsum found in Bogani Nani Wartabone National Park (North Sulawesi) and compared it with other sequences of P. pilidorsum from distinct geographical locations in Asia. The COI gene for the Sulawesi specimen was amplified using the universal primer pair LCO1490 and HCO2198. A sequence homology search was conducted through BLAST. Multiple sequence alignment was executed using CLUSTALO (1.2.1). A phylogenetic tree was constructed using the Neighbor-Joining method, and genetic distance was calculated using the Kimura 2-parameter. The COI gene sequence of the Sulawesi specimen lies in the range of 83.99-89.10% with other P. pilidorsum deposited at Gen Bank, namely KF369526 (Sarawakspecimen), AB708543, AB708544, and AB708545 (Japan specimens). The genetic distance falls in the range of 0.146-0.149 between the Sarawak specimen and the Japan specimen; 0.122-0.125 between the Sulawesi and Japan specimens; and 0.185 between the Sulawesi specimen and the Sarawak specimen. It can thus be inferred that the Sarawak and Japan specimens may not belong to the same species; the Sulawesi and Japan specimens may not belong to the same species; and the Sarawak specimen and Sulawesi specimens might be placed in different genera.
Co-Authors Abas, Abdul Hawil Adelfia Papu Aisyah, Nur’ Akbar Arafah Embo, Akbar Arafah Angelia Elisabeth Andries Asari Asari, Asari Baideng, Eva Ballamu, Larry Lowrend Basna, Mailani BEIVY JONATHAN KOLONDAM Celine Rani Kaligis Claudius F. Kairupan, Claudius F. DAMAYANTI BUCHORI Dapas, Farha D.J. Debry C Lamatoa Deidy Katili Dewi Lestari DINGSE PANDIANGAN Eka Julianti Eko Handoyo Eko Handoyo Eko Wahyu Handoyo Fabiolani, Fidelia Anninda Farha Dapas, Farha Florencia Sompie Friska S. Panjaitan Gladyes V. Leba Gobel, Iga Mawarni Grasideo V.E. Pelealu Hanny Pontororing Hapry Lapian Hard N. Pollo Hengkengbala, Sabatini Herny E.I. Simbala Hesky Stevy Kolibu Jacqueline Joseph Jayanti , Meilani Johanis Pelealu Jooudie N. Luntungan Juliana Mabe -, Juliana Mabe Jumriadi Junivia Virginia Jermias Kaligis, Celine Rani Lapian, Hapry Lino, Juniati Loho, Jesicca Lomboan, Agustinus Mamahit, Juliet Merry Eva Marina F. O. Singkoh Marnix L Langoy Marnix Langoy Martha Kawatu Masawet, Samuel Mawuntu, Estalina Mayor, Troce Mega Tilaar, Ribka Meis Nangoy Modeong, Adelia S. Mogan, Yakobus Mylton Mantouw Mylton Mantouw, Mylton Nangoy, Meis Nengsih, Reskiyah Onibala, Jane Parluhutan Siahaan Pelealu, Grasideo V.E. Pelokang, Chrisye Yustitia Pience Maabuat Pience Maabuat Pience Maabuat Pience V Maabuat Pience V Maabuat Pience Veralyn Maabuat Pience Veralyn Maabuat Pience Veralyn Maabuat Pience Veralyn Maabuat Podung, Albert Pontoh, Citra Julia Putri Tesalonika Rondonuwu Rana, Dona Cindy Elfira Ratna Siahaan Ratna Siahaan Regina R. Butarbutar Regina Rosita Butarbutar Rifgah Marmita Rivandi Patuti, Ayid Rooije R.H. Rumende Rooije R.H. Rumende Rudi Tarumingkeng Rumbay, Julia Angel Saroyo Saroyo Selvie Tumbelaka Sendy Rondonuwu, Sendy Setiawan, Denny Christian Sitti Nur Isnian Sofia Wantasen Suprianus Zega Sylvia Laatung Sylvia Laatung Taarape, Jeane Taarape, Taarape Tangapo, Agustina Monalisa Tanjung, Gadis Sabrina TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tilaar, Ribka Mega Tiltje Ransaleleh, Tiltje Tobondo, Vanda Evanglin Tri A. Mokodompit Trina E. Tallei, Trina E. TRINA EKAWATI TALLEI Trina Ekawati Tallei Trina Tallei Wakhid Wakhid Wijaya, Pandu Yudi Candra