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Program Posyandu Virtual di Masa Pandemi Mampu Menjaga Tumbuh Kembang Anak: Virtual Posyandu Program during the Pandemic Maintained Children’s Growth and Development Marchianti, Ancah Caesarina Novi; Rachmawati, Dwita Aryadina; Kusuma, Irawan Fajar; Sudarmanto, Yohanes
Amerta Nutrition Vol. 8 No. 3 (2024): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v8i3.2024.461-467

Abstract

Background: Posyandu program suspension due to COVID-19 has resulted in a decrease in monitoring children’s growth, leading to undetected problems. Understanding mothers' eating behavior plays a crucial role in preventing malnutrition, we must continue the program as a health promotion medium to enhance mothers' understanding and track children's growth and development. Objectives: This research aimed to determine the effectiveness of virtual Posyandu program in strengthening cadre health promotion services for nutritionally vulnerable children in Jember Regency agricultural area during the absence of offline Posyandu. Methods: The research was carried out September 2022-January 2023, implementing a 21-day intervention on 169 respondents from Puskesmas Arjasa and Paleran (representing two different socio-cultures). The cadres utilized the application to enhance children's nutritional health and instruct parents on its usage. The success parameter is that there were significant differences between the growth and development of children pre- and post-program. Growth variables were assessed by measuring nutritional status according to Ministry-of-Health Regulation, before and after the intervention, while development variables were assessed using a Developmental Pre-Screening questionnaire (DPSQ). Results: The virtual Posyandu program in both Puskesmas demonstrated significant differences (p<0.001) in children's growth and development parameters, such as height-for-age, weight-for-height, and DPSQ, between the pre- and post-program periods. Despite the challenges posed by the pandemic, the program has effectively maintained children's growth and development. Conclusions: Balitagrow© Android-based application in virtual Posyandu program effectively maintained children’s growth and development in pandemic era and worth continuing to be developed according to user needs.
Hubungan Tingkat Aktivitas Fisik dengan Tekanan Darah pada Buruh Tani Wanita Hipertensi di Wilayah Kerja Puskesmas Mumbulsari Jember Aisya, Meutia Citra; Jauhar Firdaus; Angga Mardro Raharjo; Aris Prasetyo; Irawan Fajar Kusuma; Ida Srisurani Wiji Astuti
CoMPHI Journal: Community Medicine and Public Health of Indonesia Journal Vol. 5 No. 2 (2024): Oktober
Publisher : Perhimpunan Dokter Kedokteran Komunitas dan Kesehatan Masyarakat Indonesia (PDK3MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/comphijournal.v5i2.256

Abstract

Hipertensi pada pekerja pertanian di Indonesia menempati urutan ke-3 teratas berdasarkan Riskesdas, 2018. Tingginya angka tersebut, dipengaruhi oleh banyak faktor, termasuk tingkat aktivitas fisik. Stigma mengenai tingginya aktivitas fisik pada pekerja pertanian sejatinya hanyalah aktivitas kerja, namun tidak untuk aktivitas lainnya, terlebih pekerja wanita yang memiliki peran ganda sehingga tidak ada waktu untuk berolahraga secara rutin. Padahal aktivitas olahraga  dapat mengontrol tekanan darah pada penderita hipertensi. Penelitian ini bertujuan untuk menganalisis ada tidaknya hubungan antara tingkat aktivitas fisik dengan tekanan darah pada buruh tani wanita hipertensi di wilayah kerja Puskesmas Mumbulsari Jember. Penelitian ini merupakan penelitian analitik observasional dengan desain cross-sectional. Sampel penelitian adalah buruh tani wanita hipertensi primer yang tinggal dan pernah melakukan kunjungan di wilayah kerja Puskesmas Mumbulsari Jember. Teknik pengambilan sampel yang digunakan yaitu total sampling sebanyak 42 sampel. Data diambil melalui wawancara kuesioner aktivitas fisik Baecke dan juga pengukuran tekanan darah menggunakan sphygmomanometer digital. Periode sampel yang diteliti adalah buruh tani wanita hipertensi yang melakukan kunjungan ke Posbindu PTM Puskesmas Mumbulsari Jember selama bulan November 2022. Data diolah menggunakan uji statistik regresi linear berganda dengan terlebih dahulu dilakukan uji asumsi klasik. Hasil uji statistik didapatkan  p-value sebesar 0,01 (p<0,05) yang menunjukkan terdapat korelasi signifikan dengan arah negatif antara aktivitas olahraga dengan tekanan darah sistolik pada buruh tani wanita hipertensi di wilayah kerja Puskesmas Mumbulsari Jember.
Impact of the User-Friendliness of BalitaGrow+ on Compliance with Children's Daily Nutrition Column Completing Pradewi, Ranny Tandria; Marchianti, Ancah C N; Shodikin, Muhammad Ali; Rachmawati, Dwita Aryadina; Kusuma, Irawan Fajar; Sudarmanto, Yohanes
Journal of Agromedicine and Medical Sciences Vol 10 No 3 (2024)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v10i3.46663

Abstract

Abstract Nutritional imbalances can lead to malnutrition which can affect children's physical and cognitive growth and development. Advances in science and technology have created opportunities for innovation in the form of health applications to help monitor children's growth and development, nutritional status, and children's daily nutritional intake. The factor that influences the intention to use technology is the ease of use. This indicates that the easier the application is to use, the higher a person's interest and compliance will be in using the application regularly. This research aims to determine the effect of ease of use of the BalitaGrow+ application on compliance with filling in the daily nutrition column for children in Rambigundam Village, Jember. This research was an analytical observational research with a cross sectional approach with a sample size of 35 people who meet the criteria. Data analysis used univariate tests and bivariate used the Spearman Rank correlation test. The results obtained from 35 respondents showed that 34 people (97.1%) found it easy to use the BalitaGrow+ application and 31 people (88.6%) had good compliance in filling in the child's daily nutrition column in the application. The results of statistical analysis showed a significance value of p value >0.05 (0.726). So it can be concluded that there was an insignificant influence between the ease of use of the BalitaGrow+ application on compliance with filling in the daily nutrition column for children in Rambigundam Village, Jember. It is suggested to increase the sample size and investigate other influenced factors for future study. Keywords: health application, ease of use, compliance of filling, children’s nutrition
Var Gene Encoding Duffy-Binding Like (DBL) 1α- Plasmodium Falciparum Erythrocyte Membrane Protein 1 (PfEMP1) as Diagnostic Marker and Clinical Predictor Candidates for Falciparum Malaria Dewi, Rosita; Sulistyaningsih, Erma; Aprilia, Annisa Nadya; Kusuma, Irawan Fajar; Sillehu, Sahrir
Althea Medical Journal Vol 11, No 4 (2024)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v11n4.2396

Abstract

Background: The pathogenesis of severe malaria involves the antigenic protein Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1), encoded by the var gene. One of the important domains in PfEMP1 is Duffy Binding Like 1α (DBL1α). To diagnose malaria, microscopic examination has low sensitivity and specificity, therefore, the development of molecular-based methods is needed. This study aimed to determine the potential of DBL1α-PfEMP1 as a diagnostic marker and clinical predictor for falciparum malaria.Methods: An exploratory descriptive study was conducted in 2019 on malaria patients at the Tiakur public health center, Southwest Maluku, Indonesia. Blood samples of patients infected with Plasmodium falciparum malaria were collected on filter paper for DNA isolation. Amplification by polymerase chain reaction (PCR) method used primers αAF [5'-GCA CG(A/C) AGT TTT GC-3'] and αBR [5'-GCC CAT TC(G/C) TCG AAC CA-3'] with cycles of denaturation 95oC 1-minute, annealing 42oC 1-minute, elongation 60oC 1-minute. PCR products were electrophoresed using 1% agarose gel. Amplicons were sequenced directly and analyzed using nucleotide BLAST-NCBI. Results: All patients showed mild malaria symptoms. PCR amplification yielded bands of 370 bp in all samples and 600 bp in 8 out of 10 samples, and 1 sample had a different pattern. Sequencing results confirmed that the amplicon was DBL1α, a var gene that had similarities to sequences from other regions.Conclusion: Positive amplification and sequencing results confirm the sensitivity of DBL1α-PfEMP1 as a diagnostic marker. The sequence variability of PCR product implies the presence of DBL1α variations, indicating a correlation with clinical outcomes and making it a clinical predictor.
EVALUATION OF BRAIN MICROGLIA PROLIFERATION AS A RESPONSE TO DBL2β-PFEMP1 RECOMBINANT PROTEIN IMMUNIZATION IN WISTAR RAT Dewi, Rosita; Kusuma, Irawan Fajar; Sulistyaningsih , Erma; Rachmania, Sheilla; Luthfiyyah, Nur Atikah
MNJ (Malang Neurology Journal) Vol. 11 No. 1 (2025): January
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2025.011.01.04

Abstract

Background: In the malaria vaccine study, Duffy binding-like 2β Plasmodium falciparum erythrocyte membrane protein 1 (DBL2β-PfEMP1) could induce the  IgG and CD4+production. Antibody to DBL2β-PfEMP1 reduces the risk of developing severe malaria through the blockade of cytoadherence and destruction of rosette formation. During the process of antibody formation after immunization, the released peripheral cytokines have the potential to cause blood-brain barrier disruption resulting in the activation and proliferation of brain microglia as primary innate immune cells leading to neuroinflammation. Objective: This study aims to evaluate brain microglia proliferation as a response to recombinant protein DBL2β-PfEMP1 immunization in Wistar rats. Methods: Wistar rats were injected subcutaneously with recombinant protein DBL2β-PfEMP1 at doses of 100, 150, and 200 µg/kgBW on days 0, 21, and 42. Rats were euthanized on day 56. Brain histopathological slides were prepared and stained using hematoxylin-eosin. Histological examination was performed using a microscope at 400X magnification and documented using an AmScope microscope digital camera. Brain microglia were calculated using Fiji Image-J. The data were statistically analyzed using the Kruskal-Wallis test.  Results: The average number of brain microglia in both the control and treatment groups was 82–88. There was no significant difference in brain microglia number between the control and treatment groups (p>0.05) after recombinant protein DBL2β-PfEMP1 immunization. Conclusion: Recombinant protein DBL2β-PfEMP1 immunization does not provoke the proliferation of brain microglia in Wistar rats. This suggests that the protein does not have the potential to cause neuroinflammation.
Soil-Transmitted Helminth Infection and Macronutrient Intake among Stunted Toddlers in Panti Sub-district, Jember Ghaiska Najma Amnur; Yunita Armiyanti; Irawan Fajar Kusuma; Leersia Yusi Ratnawati; Wiwien Sugih Utami; Bagus Hermansyah
Media Gizi Indonesia Vol. 20 No. 1 (2025): MEDIA GIZI INDONESIA (NATIONAL NUTRITION JOURNAL)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgi.v20i1.74-80

Abstract

Soil-transmitted helminth (STH) infections are one of the most common infectious diseases in the developing world, with prevalence rates as high as 65%. STH infections generally affect the digestive system, which can reduce appetite and nutrient absorption. This study aimed to determine the association between STH infection and macronutrient intake in stunted toddlers aged 12-36 months in Panti Sub-district, Jember Regency. This study used observational analysis method with cross-sectional approach. The total number of subjects was 83 stunted toddlers. Data on STH infection were collected through fecal examination and 2x24 hour food recall questionnaire to assess their macronutrient intake. The results of the study showed that the prevalence of STH was 4.8% with species identified in the examinations such as Ascaris lumbricoides (2.4%) and hookworm (2.4%). The majority of macronutrient intake was considered sufficient for protein (77.1%), deficit for carbohydrate (94%) and fat (77.1%). Bivariate analysis using the contingency coefficient correlation test showed that there was no significant correlation between STH infection and macronutrient intake. Based on the results of this study, macronutrient intake may be influenced by other factors such as the role of parents in preparing nutritious food, economic status, and mild degree of infection. However, the underlying mechanism remains unclear and should be further investigated.
Evaluation of Kidney Proximal Tubule Following Immunization with Plasmodium falciparum CIDR1α-PfEMP1 Recombinant Protein in Rats Dewi, Rosita; Sulistyaningsih, Erma; Kusuma, Irawan Fajar; Rachmania, Sheilla; Rahma, Nafisah Hani Asyifah
Majalah Kedokteran Bandung Vol 57, No 1 (2025)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v57.3801

Abstract

Malaria vaccines are continuously explored as an approach to eradicate malaria. The cysteine-rich interdomain region 1α-Plasmodium falciparum erythrocyte membrane protein 1 (CIDR1α-PfEMP1) is an antigenic protein that can bind to the endothelial protein C receptor (EPCR) and CD36, resulting in microvascular obstruction. The PfEMP1-induced antibody can induce antibodies, reducing the severity of malaria risk by impeding cytoadherence and destructing rosette formation. Preclinical safety testing is an important step of vaccine development, including safety testing of the kidney as the main excretory organ. The proximal tubule has the most mitochondria to support its main role in reabsorption and excretion, making it prone to oxidative stress caused by foreign substances. This study aimed to evaluate kidney proximal tubule cells after CIDR1α-PfEMP1 immunization in rats. This study was conducted at the Laboratory of Biology Molecular and Biotechnology, Faculty of Medicine, University of Jember. Eight rats were injected subcutaneously with 150 µg of the protein and four rats were injected with 0.9%  NaCl on days 0, 21, and 42. The rats were euthanized on day 56. The kidney histopathological slides were stained using Hematoxylin-Eosin (HE) and the necrotic proximal tubule cells were counted at five (5) visual fields (100 cells/visual fields). The average number of necrotic cells of the control and the treatment groups were 0.125±0.25 and 2.438±2.5972 while the Mann-Whitney test showed a significance value of p=0.12,  indicating no significant difference between the control and treatment groups. In conclusion, there is no change in the kidney histopathology based on the proximal tubule necrotic cell count after CIDR1α-PfEMP1 immunization in rats.
Differential Leukocyte Count Responses Post Injection of Duffy-binding-like Domain-2β of PfEMP1 Recombinant Protein in Wistar Rat Zahniar, Zahniar; Sulistyaningsih, Erma; Rachmania, Sheilla; Dewi, Rosita; Kusuma, Irawan Fajar
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.628

Abstract

Malaria due to Plasmodium falciparum causes a high mortality rate, and vaccination is a valuable approach to control it. One malaria vaccine candidate is Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1), specifically Duffy binding-like 2β (DBL2β) domain (DBL2β-PfEMP1), which has a vital role in severe malaria pathogenesis. The DBL2β-PfEMP1 recombinant protein is immunogenic. This study aimed to investigate the immune response of DBL2β-PfEMP1 protein by analyzing the differential leukocyte count. Twenty-three rats were randomly divided into control and five treatment groups. Rats were injected on days 0, 21, and 42 with a physiological solution of 0.9% NaCl, adjuvant, DBL2β-PfEMP1 protein, and each mixture of DBL2β-PfEMP1 protein with doses of 150, 300, and 450 µg/200gBW and adjuvant. Blood was collected on day 56 and prepared for differential leukocyte count examination with a visual microscopic examination by two expert observers. The results showed that DBL2β-PfEMP1 recombinant protein and adjuvant increased the eosinophils and neutrophils but decreased monocytes and lymphocytes and did not affect the basophils. Statistical analysis showed significant differences between groups for eosinophils (between control and DBL groups; Adj and DBL groups; DBL and other groups except DBL150+adj) and monocytes (between control and all doşe groups with adjuvant; DBL and all doşe groups with aduvant), but not for basophils, neutrophils, and lymphocytes. In conclusion, the serial injection of DBL2β-PfEMP1 recombinant protein showed different responses in each leukocyte cell type. Further analysis by time-series differential leukocyte count examination will be essential to determine the responses of each type of leukocyte to support the research on malaria vaccine development.
Molecular Method Optimization to Identify Plasmodium falciparum Multidrug Resistance 1 (pfmdr1) gene as a Predictor of Antimalarial Resistance Sulistyaningsih, Erma; Dewi, Rosita; Rachmania, Sheilla; Kusuma, Irawan Fajar; Kholifaturrohmy, Muhammad Rizqi; Armiyanti, Yunita; Kholifaturrohmah, Sakinatus Sariroh; Andriani, Made Prasanti
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.673-678

Abstract

Several approaches have been designed to control malaria, a disease with high morbidity and mortality, but they face some hurdles. Antimalarial resistance is one of the major challenges for malaria elimination, so the detection of antimalarial resistance is essential. Several molecular markers for antimalarial resistance have been identified, including Plasmodium falciparum multidrug resistance 1 (pfmdr1) gene. This study determined the optimization of molecular techniques to identify the pfmdr1 gene as an antimalarial resistance predictor in Indonesia. The study included patients diagnosed with uncomplicated or severe malaria originating from the health district of Kerom Regency, Papua Province, and Dr. Soebandi Hospital, Jember, East Java Province. Blood samples were collected in the Whatmann filer paper after informed consent. DNA was isolated from dried blood filter paper, and nested PCR was performed using a specific primer, the pfmdr1-A and pfmdr1-B genes. The PCR cycle was optimized based on previous studies. The pfmdr1-A has a similar setting to the earlier study, but the pfmdr1-B had a different optimum setting from the previous study with the annealing temperature of 57oC for nested-1 and 62oC for nested-2. This PCR setting could be used for further examination. The positive results of the amplification indicated the potential for antimalarial resistance in the parasite population. A study on the gene copy number and polymorphism is essential to determine the definitive conclusion on antimalarial resistance.
Tinjauan Sistematik: Efektivitas dan Keamanan Fecal Microbiota Transplantation pada Infeksi Clostridium difficile Berulang pada Anak Rosdiana, Fetri; Suswati, Enny; Kusuma, Irawan Fajar
Majalah Kedokteran Indonesia Vol 72 No 4 (2022): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.72.4-2022-520

Abstract

Background: Recurrent Clostridium difficile infection (RCDI) continues to increase in incidence and severity around the world. Fecal microbiota transplantation (FMT) is commonly used to treat RCDI in adults, but its efficacy and safety in the pediatric population are infrequently studied. Here, we performed a systematic review to evaluate the efficacy and safety of FMT for RCDI in children.Methods: Systematic literature search was performed using PubMed, Science Direct, Nature, Cochrane Library, and Springer to identify original studies in English language published from 2010-2020. The clinical resolution, bacterial resolution, and failure of therapy were calculated as indicators of efficacy of FMT. Adverse events were also calculated as indicators of FMT safety.Results: A total of 21 studies, with 498 pediatric patients were included in this review. There were 418/498 (84%) patients reported clinical and bacterial resolution. There were 67/498 (13%) patients who experienced FMT-related adverse events, with 97% of them had mild adverse event. There was one patient who reported FMT-related serious adverse events which were aspiration pneumonia and dehydration.Conclusion: We found evidence that supports the efficacy and safety of the use of FMT for treatment of recurrent C. difficile infection in children.
Co-Authors Adelia Handoko Adhitya Wicaksono Aisya, Meutia Citra Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Aprilia, Annisa Nadya Aris Prasetyo Arsyzilma Hakiim Ayu Yoniko Ayu Yoniko Christiari Bagus Hermansyah Dini Agustina Dwita Aryadina Rachmawati ENNY SUSWATI Erma Sulistyaningsih Erwin Nur Rif'ah Ghaiska Najma Amnur Ida Srisurani Wiji Astuti Jauhar Firdaus Kautsar, Azyumardi Azra Khoiruddin, Mukhammad Arif Hadi Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Khulaida Fatila Hayati Kusuma, Ancah Caesarina Leersia Yusi Ratnawati Luthfiyyah, Nur Atikah M. Ali Shodikin Marchianti, Ancah C N Muhammad Hasan Muhammad Hasan Muhammad Hasan Nurmaida, Eny Pradewi, Ranny Tandria Putra, Muhamad Dwi Eka Putri, Elvia Rahmi Marga Ragil Ismi Hartanti Rahma, Nafisah Hani Asyifah Ramzi Syamlan Rizka Kusumaningsih Roan Pratama Putra Rosdiana, Fetri Rosita Dewi Sa’diyah, Nindya Audatus Setyoadji, Winnie Almira Sheilla Rachmania, Sheilla Sillehu, Sahrir Sudarmanto, Yohanes Sulistyaningsih , Erma Suyani Indriastuti Tantut Susanto Tartila, Jasmine Wiwien Sugih Utami Yudha Nurdian Yunita Armiyanti Zahniar, Zahniar