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All Journal Berkala Ilmiah Pendidikan Biologi (BioEdu) LenteraBio Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Biodjati Jurnal Biologi Tropis JURNAL PENDIDIKAN MIPA Jurnal Abdi: Media Pengabdian Kepada Masyarakat BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia JIPI (Jurnal IPA dan Pembelajaran IPA)
Lisa Lisdiana
Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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BIODIVERSITY OF CELLULOLYTIC BACTERIA ISOLATED FROM FERMETODEGE FOR RUMINANTS Isnawati Isnawati; Lisa Lisdiana; Mahanani Tri Asri; Guntur Trimulyono
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1904

Abstract

ARTICLE HIGLIGHTS- Fermetodege as a source of cellulolytic bacteria that degrade cellulosic materials.- Enhances ruminant feed by improving palatability and digestibility.- Utilizes water hyacinth, reducing invasive plant spread and supporting ecosystems.- Bioprospecting reveals new bacteria for effective fermentation starter development.ABSTRACTFermetodege is a name of fermented ruminant feed produced from free heavy metals water hyacinth, rice bran, and corn cobs. Its quality, as well as the fermentation process, are affected by numerous factors, such as the diversity, evenness, and dominance of indigenous cellulolytic bacterial species. A proper understanding of these influential factors is needed to improve the quality of the fermented feed. Therefore, this study aims to evaluate the diversity, evenness, and dominance of cellulolytic bacteria isolated from fermetodege. The cellulolytic activity of the isolates was tested by observing their growth on the carboxymethylcellulose (CMC) media. Isolates with cellulolytic activity formed a clear zone after the colonies were soaked with 0.1% Congo red and rinsed with 1 M NaCl. The Shannon-Weiner's diversity and evenness indexes, as well as Simpson's species dominance, were then calculated. The result showed that the Shannon-Weiner’s diversity index ranged from 0.6849 to 1.8173, and it was categorized as medium. The evenness index was between 0.7778 and 0.9983, which indicates a stable distribution of species. Meanwhile, the Simpson’s species dominance ranged from 0.1835 to 0.5082, which implies that none of the species was dominant. These results show that fermetodege is potentially a source of bacterial isolates and can be used as a fermentation consortium starter.
IDENTIFICATION OF BACTERIA FROM FOOD COURT WASTEWATER BASED ON 16S rRNA GENE SEQUENCES Indah Nur Elasari; Rihan Ali Abdillah; Satria Prasna Dewa; Jafni Amanda Kinarossa Widjaja; Lisa Lisdiana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13541

Abstract

Environmental safety and public health are greatly influenced by the microbiological elements such as bacterial community, where these organisms can be used as indicators of environmental quality and sources of potential biological hazards. The identification of bacteria in wastewater is an important aspect of environmental microbiology studies. The purpose of this study was to identify the bacterial species in food court wastewater at State University of Surabaya (UNESA) Campus 1. The methods used were exploratory, including bacterial cultivation, DNA extraction, quantitative and qualitative DNA analysis, amplification of 16S rRNA gene fragments, sequencing, and phylogenetic tree reconstruction. From phylogenetic analysis, five test isolates were related to the families Enterobacteriaceae, Aeromonadaceae, Chromatiaceae, and Halomonadaceae. Isolates BU4 and BU5 were most closely related to genus Aeromonas with a similarity of 98.60% and 97.27%, respectively, while isolate BU3 was closely related to genus Klebsiella with a similarity of 96.91%. Meanwhile, isolates BU1 and BU2 cannot yet be identified into a bacterial genus. The results of this study provide important information regarding the prediction of pathogenic bacteria types, which can serve as the basis for recommending further action to prevent disease, improve water quality, and increase the sterility of the food court area.
IDENTIFICATION OF BACTERIA FROM FOOD COURT WASTEWATER BASED ON 16S rRNA GENE SEQUENCES Indah Nur Elasari; Rihan Ali Abdillah; Satria Prasna Dewa; Jafni Amanda Kinarossa Widjaja; Lisa Lisdiana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13541

Abstract

Environmental safety and public health are greatly influenced by the microbiological elements such as bacterial community, where these organisms can be used as indicators of environmental quality and sources of potential biological hazards. The identification of bacteria in wastewater is an important aspect of environmental microbiology studies. The purpose of this study was to identify the bacterial species in food court wastewater at State University of Surabaya (UNESA) Campus 1. The methods used were exploratory, including bacterial cultivation, DNA extraction, quantitative and qualitative DNA analysis, amplification of 16S rRNA gene fragments, sequencing, and phylogenetic tree reconstruction. From phylogenetic analysis, five test isolates were related to the families Enterobacteriaceae, Aeromonadaceae, Chromatiaceae, and Halomonadaceae. Isolates BU4 and BU5 were most closely related to genus Aeromonas with a similarity of 98.60% and 97.27%, respectively, while isolate BU3 was closely related to genus Klebsiella with a similarity of 96.91%. Meanwhile, isolates BU1 and BU2 cannot yet be identified into a bacterial genus. The results of this study provide important information regarding the prediction of pathogenic bacteria types, which can serve as the basis for recommending further action to prevent disease, improve water quality, and increase the sterility of the food court area.
Detection of Porcine Contamination in Beef Meatballs from Gedangan District Using PCR–RFLP Gian Firdaus Putra Suryadi; Rahma Windi Astuti; Lilis Nur Fitriani; Lisa Lisdiana
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49813

Abstract

Meatball is one of the popular meat dishes in Indonesia, especially in Sidoarjo. The sale of meatballs in Sidoarjo is carried out by Micro, Small, and Medium Enterprises (MSMEs), with 330 of the total 11,114 micro-traders selling meatballs. However, there is a risk of pork being adulterated into meatballs. This is a serious problem because Indonesia, especially Sidoarjo, is a Muslim-majority city with 95.5% of the total population of 2 million. Therefore, the purpose of this article is to conduct field checks to determine whether or not pork contamination is present in meatballs in Sidoarjo. Sampling locations were determined using a stratified random sampling method, which found that eight of the 15 villages, including Karangbong, Tebel, Sruni, Keboananom, Keboansikep, Gedangan, Ketajen, and Sawotratap Villages, have a significance value greater than the alpha value (0.05). Therefore, eight of them were used as sampling locations. One sample was purchased from each of the eight chosen villages, named S1, S2, S3, S4, S5, S6, S7, and S8. Meanwhile, the possibility of porcine contamination in beef meatball samples was analyzed using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method with the restriction enzyme BsaJI. This study found that all eight samples and the negative control DNA amplicons were not cleaved by the BsaJI enzyme. Meanwhile, the positive control DNA amplicon was cleaved into 3 fragments. It was indicated that all samples and the negative control DNA amplicons were free of pork DNA contamination. Despite the anomaly, it can be concluded that all meatball samples bought from Gedangan District are not contaminated with pork. By demonstrating that meatballs from Sidoarjo are free from pork DNA, this study serves as an additional halal food monitoring in Sidoarjo. It reinforces public trust in local MSME food products.
Efektivitas Isolasi DNA Bakteri Escherichia coli dengan Modifikasi Buffer lisis Spin Column Fariska Amadea Julia; Khoirun Nisa; Lailatul Maghfiroh; Azza Irani Dwi Lestari; Natasya Dwi Anjani; Lisa Lisdiana
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 8 No 2 (2026): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2026.8.2.17743

Abstract

Isolasi DNA merupakan tahapan penting yang mendasari penelitian lanjut dalam berbagai aplikasi bioteknologi dan penelitian molekuler, termasuk kloning gen, analisis PCR, dan sekuensing DNA. Isolasi DNA dapat dilakukan menggunakan kit, salah satunya adalah kit spin column. Penggunaan kit spin column dalam isolasi DNA lebih efektif dan efisien karena bahan-bahan yang diperlukan dan prosedur penggunaan kit telah tersedia dalam satu paket, sehingga penggunaan kit lebih cepat dan mudah. Namun, pada saat menggunakan kit adakalanya bahan yang digunakan, seperti buffer lisis habis dan memerlukan bahan pengganti agar proses isolasi DNA tetap berjalan. Sehingga, diperlukan adanya pembuktian bahwa bahan pengganti dari kit spin column memiliki hasil dan kualitas yang sama dengan kit spin column. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas dari SDS 20% sebagai pengganti pada kit spin column berdasarkan evaluasi kuantitas DNA yang dihasilkan. Metode dilakukan sesuai dengan prosedur kit spin column dan memodifikasi buffer lisis dengan menggunakan bahan SDS 20%. Hasil penelitian menunjukan bahwa tingkat konsentrasi DNA dari modifikasi SDS lebih tinggi (13,9 ng/µL) dibandingkan konsentrasi DNA dari kit spin column (3 ng/µL). Hal ini disebabkan karena SDS memiliki kemampuan yang kuat dalam melisiskan sel. Namun, tingkat kemurnian DNA hasil dari kit spin column dan modifikasi menggunakan SDS 20% memiliki nilai yang sama, yaitu sekitar 1,8. DNA yang dianggap murni dan minim kontaminasi protein menunjukan nilai kemurnian 1,7-2,0. Dengan demikian, modifikasi buffer lisis pada kit spin column dengan SDS 20% dapat menjadi alternatif yang efektif untuk diterapkan dalam laboratorium. Kata kunci : Buffer lisis; Escherichia coli; Kemurnian DNA; Modifikasi; SDS; Spin column
Perbandingan Cemaran Escherichia coli Pada Ikan Lele (Clarius gariepinus) dari Dua Lokasi Budidaya di Blitar Fera Agustine Rahmawati; Arif Muttaqin; Lisa Lisdiana
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 8 No 2 (2026): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2026.8.2.17751

Abstract

Lele (Clarias gariepinus) merupakan salah satu komoditas ikan air tawar yang digemari oleh masyarakat Indonesia, termasuk di Blitar. Tingginya konsumsi masyarakat mendorong peningkatan produksi lele. Lele yang relatif tahan terhadap lingkungan kurang baik seperti air kotor, keruh, dan oksigen terbatas, sering dimanfaatkan pembudidaya untuk memudahkan pemeliharaan dengan tetap memperoleh hasil panen. Akan tetapi, kondisi tersebut berpotensi mengalami cemaran patogen termasuk Escherichia coli yang dapat membahayakan kesehatan konsumen. Penelitian ini bertujuan melakukan identifikasi awal mengenai cemaran E. coli pada lele dari dua lokasi budidaya di Blitar. Sampel yang digunakan masing-masing 3 ekor lele yang berasal dari budidaya Kelurahan A dan Desa B. Pengujian cemaran E. coli menggunakan metode MPN mengacu SNI 01-2332.1-2006. Hasil uji didapatkan adanya cemaran E. coli di kedua budidaya, di budidaya Kelurahan A sebanyak 93 hingga lebih dari 1100 MPN/gram dan Desa B sebanyak 3,0 hingga 9,2 MPN/gram. Berdasarkan SNI 7388:2009, seluruh sampel lele melebihi batas maksimum cemaran E. coli sehingga tidak memenuhi standar mutu mikrobiologis ikan segar. Namun, sumber dari kontaminasi tersebut masih belum bisa ditentukan pada penelitian ini.Kata kunci: Escherichia coli, Cemaran mikrobiologis, Clarias gariepinus, Akuakultur, MPN
The Potential of Teak Forest Local Wisdom as a Learning Resource in Science, Technology, Engineering, and Mathematics Education: A Bibliometric Analysis of Problem-Solving Skills Chozy Eka Nur Yahya; Endang Susantini; Lisa Lisdiana; Muhammad Zahrudin Afnan; Corrienna Abdul Talib
Jurnal IPA & Pembelajaran IPA Vol. 10 No. 3: SEPTEMBER 2026
Publisher : Universitas Syiah Kuala, in collaboration with the Perkumpulan Pendidik IPA Indonesia (PPII), (MoU)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v10i3.2421

Abstract

The integration of science, technology, engineering, and mathematics (STEM) learning with local wisdom has received increasing attention. However, its potential for utilizing local wisdom as an authentic learning context still requires further investigation. This study aims to map research trends in local wisdom-based STEM learning, examine research themes and their relationship with sustainable development goals (SDGs) 4 and 15, and analyze the potential of teak forest local wisdom in STEM learning to enhance students’ problem-solving. This study employed a bibliometric approach combined with a systematic literature review (SLR). Data were obtained from Scopus indexed articles published between 2006-2026 through the stages of data retreival, screening, data cleaning, analysis, visualization, and interpretation. Bibliometric analysis was conducted using microsoft excel, VOSviewer, and biblioshiny. The results indicate that publications on local wisdom-based STEM learning have increased, particularly during the 2024-2025 period, and are dominated by themes related to science education, STEM, local wisdom, and environmental issues. The emerging themes demonstrate a relationship between SDGs 4 and 15. However, the use of terrestrial ecosystems as a context for STEM learning and problem-solving skills (PSS) has not yet become a major focus. The synthesis results show that the local wisdom employed is contextual, closely related to students’ environments, and incorporates real world problems. Therefore, teak forest local wisdom has the potential to serve as an authentic context in STEM learning to support PSS while simultaneously connecting SDGs 4 and 15
Pengembangan Media Pembelajaran Kartu Exploding Genetics untuk Meningkatkan Pemahaman Peserta Didik pada Materi Substansi Genetik Andreas Stefano; Lisa Lisdiana
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 15 No. 3 (2026)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v15n3.p873-884

Abstract

Mata pelajaran Biologi adalah suatu mata pelajaran yang kompleks karena banyaknya konsep dan istilah ilmiah yang harus dimengerti. Satu dari banyaknya materi yang bersifat kompleks pada mata pelajaran Biologi yaitu materi Substansi Genetik. Sulitnya memahami materi Substansi Genetik disebabkan oleh sifatnya yang abstrak, karena materi yang dikaji memiliki ukuran mikroskopis dan banyak istilah yang perlu dihafal dan dipahami. Penelitian ini memiliki tujuan untuk mengembangkan kartu Exploding Genetics sebagai media pembelajaran yang dapat meningkatkan kemampuan peserta didik dalam memahami materi Substansi Genetik, dilanjutkan mendeskripsikan seberapa valid, praktis, dan efektif kartu Exploding Genetics yang dikembangkan. Studi ini mengadopsi model pengembangan ADDIE dengan tahap Analysis, Design, Development, Implementation, dan Evaluation. Kartu yang telah dikembangkan kemudian melalui tahap validasi dan diujicobakan secara terbatas kepada 20 peserta didik di SMAN 7 Kediri. Proses pengambilan data menggunakan metode validasi, observasi, tes, dan angket. Kartu Exploding Genetics yang dikembangkan dinyatakan valid untuk diujicobakan dengan persentase sebesar 86,40%, berdasarkan aspek penyajian, isi, dan kebahasaan. Kartu Exploding Genetics dinyatakan praktis dengan persentase keterlaksanaan sebesar 85,71% dan persentase hasil respon siswa sebesar 98,46%. Kartu Exploding Genetics dinyatakan efektif dengan persentase ketuntasan peserta didik mencapai 70% dan gain score mencapai 0,52 (kategori sedang) berdasarkan hasil belajar kognitif siswa. Dari hasil penelitian tersebut, disimpulkan bahwa media pembelajaran kartu Exploding Genetics yang dikembangkan untuk meningkatkan pemahaman siswa pada materi Substansi Genetik, dinyatakan valid, praktis, dan efektif.
Analysis of DNA Quality in Nile Tilapia (Oreochromis niloticus) Liver Tissue Based on Differences in Distribution Sources (Traditional Markets and Supermarkets) Rahayu Endarwati; Adinda Revalina Putri; Moh. Arif Setiawan; Tiana Belinda Maharani; Regina Martha Ayu; Rossa Indira Rahmadhini; Erlix Rakhmad Purnama; Lisa Lisdiana
Jurnal Biologi Tropis Vol. 26 No. 4 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i4.12600

Abstract

DNA quality is an important molecular parameter that reflects the integrity of an organism’s genetic material. Nile tilapia (Oreochromis niloticus) is a high-value aquaculture commodity distributed through various marketing channels, including traditional markets and modern supermarkets. Differences in postharvest handling practices, storage conditions, and hygiene standards may influence the biological quality of fish, although these factors were not directly evaluated in this study. This study aimed to compare the DNA quality of Nile tilapia liver tissue obtained from traditional markets and supermarkets through DNA isolation using the meBEP kit, measurement of DNA concentration and purity with a NanoDrop spectrophotometer, DNA amplification by PCR, and visualization using agarose gel electrophoresis. NanoDrop measurements showed that samples from the traditional market (N1) had higher DNA concentrations (50.8–56.8 ng/µL) than samples from the supermarket (N2), which ranged from 3.1 to 6.1 ng/µL. The A260/A280 ratio of group N1 was more stable and closer to the expected range for pure DNA (1.63–1.66) than that of group N2 (0.78–1.54). However, all samples exhibited A260/A230 ratios well below the ideal value, indicating the presence of non-protein contaminants in the DNA isolates. Agarose gel electrophoresis showed no visible DNA bands for either the isolated DNA or the PCR products in both sample groups. These results indicate that, under the isolation and amplification conditions used in this study, the extracted DNA was of insufficient quality to serve as a reliable PCR template. The observed differences between sample groups should therefore be interpreted descriptively, as postharvest factors such as distribution time and storage temperature were not measured.
Co-Authors Addabiyah, Sayyidatul Adinda Revalina Putri AMANAH, ISKHAWATUN Ana Mariatul Khiftiyah Andreas Stefano Anggi Maulia Arista Anggraini, Lusiana Anissa, Ratih Khairul Annafilah, Adinda Anisah Arif Muttaqin Arista, Anggi Maulia Arista, Anggi Maulia Asri, Mahanani Tri Azza Irani Dwi Lestari Bima Purnama Putra BUDIYANTI SUMPETHANAYA, DWILIAM Budiyanti Catur Wardeny Chozy Eka Nur Yahya Corrienna Abdul Talib Dwi Nurhayati Adhani, Dwi Nurhayati Eggy Ramanda Putra Endang Susantini Erlix Rakhmad Purnama Eva Ari Wahyuni Fariska Amadea Julia Fera Agustine Rahmawati Fitriari Izzatunnisa Muhaimin Gian Firdaus Putra Suryadi Guntur Trimulyono Hardaning Pranamuda HERYANI PUTRI, EGA Heryani HIDAYATULLAH, FUAD Husna, Asmaul Indah Nur Elasari Intan Puspita Maharani Isnawati Isnawati Isnawati Isnawati Iswanti, Anisah Jafni Amanda Kinarossa Widjaja Jamhari Jamhari Jusup Subagja Jusup Subagja, Jusup Khilma Ziyadatur Rizka Maulida KHOIRUN NISA Lailatul Maghfiroh Langkah Sembiring LANGKAH SEMBIRING Lilis Nur Fitriani Mahanani Tri Asri Mahanani Tri Asri Maulida, Khilma Ziyadatur Rizka Meilinda, Alfina Moh. Arif Setiawan Muhammad Zahrudin Afnan MUKAMTO MUKAMTO Natasya Dwi Anjani NOVITA KARTIKA INDAH Novita Kartika Indah Pramesti, Ertysha Salya Rahayu Endarwati Rahayu, Yuni Sri Rahma Windi Astuti Ratih Khairul Anissa Regina Martha Ayu Rihan Ali Abdillah Rinie Pratiwi Puspitawati Rossa Indira Rahmadhini Saputri, Anggieta Wahyu Satria Prasna Dewa Sifa’ Daru Quthnie Nadaa Zakiyah Tiana Belinda Maharani Tri Ardyati Tri Ardyati Valentina, Fithriani Vivin Nur Fitria Wardeny, Catur Wisanti Wisanti Wisanti Wisanti Yolanda Hanani Shofiyullah Yuliani Yuliani Yuliani, Yuliani Yuliani, Yuliani YUNI SRI RAHAYU ZUMRONA, HILDA