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STRUKTUR DAN ASOSIASI JENIS LAMUN DI PERAIRAN PULAU-PULAU HIRI, TERNATE, MAITARA DAN TIDORE, MALUKU UTARA Yunita Ramili; Dietriech G. Bengen; Hawis Madduppa; Mujizat Kawaroe
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 3 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.116 KB) | DOI: 10.29244/jitkt.v10i3.22476

Abstract

ABSTRAKKeberadaan ekosistem lamun di perairan pesisir pulau-pulau kecil berperan penting sebagai habitat dan penyedia sumber daya ikan, serta pelindung garis pantai dan daratan pulau-pulau kecil tersebut. Penelitian ini bertujuan untuk menganalisis sebaran, komposisi, kerapatan, penutupan dan asosiasi jenis lamun di perairan pulau-pulau kecil Hiri, Ternate, Maitara dan Tidore, Maluku Utara. Pengambilan data dengan menggunakan metode transek garis dan transek kuadrat. Selanjutnya data dianalisis dengan menggunakan bantuan perangkat lunak MS Excel dan XLstat. Hasil penelitian menunjukkan bahwa Pulau Tidore memiliki jumlah jenis lamun terbanyak yakni delapan jenis lamun dari sembilan jenis lamun yang ditemukan di seluruh lokasi penelitian. Tiga jenis lamun yaitu, Enhalus acoroides, Thalassia hemprichii dan Cymodocea rotundata, menyebar luas dan terdapat di keempat pulau tersebut. Komposisi, kerapatan dan penutupan jenis lamun bervariasi antar stasiun penelitian. Vegetasi lamun yang ditemukan di lokasi penelitian berupa vegetasi campuran yang terdiri dari tiga sampai delapan jenis lamun. E. acoroides banyak ditemukan di Stasiun Mtr1 dan Tte2 tidak berasosiasi dengan spesies lainnya, demikian juga dengan C. serrulata yang ditemukan di Stasiun Mtr2 dan T. hemprichii di Stasiun Tdr2 dan Tdr3. Asosiasi C. rotundata dan Syiringodium isoetifolium terlihat di stasiun Tdr1 dan Hr1, sementara Halophila ovalis dan H. spinulosa  tidak menunjukkan asosiasi dengan jenis lamun lainnya di lokasi penelitian. Secara keseluruhan kondisi lingkungan perairan di keempat pulau tersebut masih tergolong baik dan mampu mendukung ekosistem lamun. ABSTRACTThe existence of seagrass ecosystems in the coastal region of small islands has been playing an essential role as a habitat and the supplier of fish resources, as well as a shore and coastline protector of small islands. This study aimed to  determine the distribution, composition, density, coverage, and associations of seagrass plant in the islands of Hiri, Ternate, Maitara, and Tidore. Data were collected by using line transect method and quadrate transect. Furthermore, data were analyzed by using MS Excel and XLstat software. The results showed that Tidore Island has the highest number of seagrass species namely eight from nine species of seagrasses found in all research sites. Three species of seagrasses, Enhalus acoroides, Thalassia hemprichii, and Cymodocea rotundata, were widespread in all four islands. The composition, density and coverage of seagrass species varied among research stations. Vegetation of seagrasses found in the study site in the form of mixed vegetation consisting of three to eight species. Enhalus acoroides found mostly in Mtr1 and Tte2 stations were not associated with other seagrass species, nor Cymodocea serrulata that found in Mtr2 Station and T. hemprichii at Tdr2 and Tdr3 stations. The associations of C. rotundata and Syringodium isoetifolium were observed at Tdr1 and Hr1 Stations. While Halophila ovalis and Halophila spinulosa showed no association with other seagrass species at the study area. Overall the condition of the marine environment on the four islands is still relatively good and able to support the life of the seagrass ecosystem.
DNA barcoding of the soft coral, Clavularia inflata, shows two major groups across Indonesian coral reefs Beginer Subhan; Dietriech G. Bengen; Sebastian C. A. Ferse; Fauzan Dzulfannazhir; Luzmi Malia Izza; Nurlita Putri Anggraini; Prakas Santoso; Dondy Arafat; Lalu M. Iqbal Sani; Hawis H. Madduppa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 1 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.1.1-12

Abstract

Clavularia inflata was first described from Ternate Island, Indonesia in 1896 and later reported appeared from Japan and Taiwan in 1953.Clavularia (Blainville 1830) soft corals exhibit complex morphological traits that are difficult to differentiate, thus complicating their identification. DNA barcoding has been envisioned and actively pushed as a credible method for assigning unidentified specimens to known species by comparison to a molecular reference data database. Thus, the purpose of this study was to use molecular methods to confirm the identity of 25 colonies taken from 13 Indonesian coral reef sites and putatively identified as Clavularia inflata. All specimens were identified as Clavularia inflata molecularly using the mitochondrial DNA mtMuts gene. Although a comparison of the nucleotide base chains to Genbank data indicates that the samples belong to a single species, two clades in the phylogenetic tree and data from the Automatic Barcode Gap Discovery (ABGD) indicate that there are two major groups of C. inflata in Indonesia, implying cryptic species.
Glutathione (GSH) Production as Protective Adaptation Against Light Regime Radiation of Symbiodinium Natural Population Moh Muhaemin; Dedi Soedharma; Hawis H Madduppa; Neviaty Putri Zamani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 3 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11.588 KB) | DOI: 10.14710/ik.ijms.22.3.121-125

Abstract

Glutathione (GSH), as a wide range of low molecular weight, which found in marine microalgae and event bacteria, are essential to prevent photooxidation and productivity loss from these Radical Oxigen Species (ROS). Symbiodinium, endo-symbiont of corals, were exposed with different UV radiation combined with irradiance treatments to explore biomass specific initial response. Intracellular glutahione was observed as potential adaptive response of Symbiodinium population under environmental specific stress. The result showed that GSH production increased significantly with increasing irradiance and/or UV levels. GSH concentration was fluctuated among populations exposed by different irradiance treatments, but not effected by UV and irradiance exposure. GSH production as a response of UV exposure was higher than irradiance treatments. Both these high correlative fluctuation of intracellular GSH production and the presence of both treatments indicated protective specific adaptation of Symbiodinium under specific environmental stress, respectively. Keywords: zooxanthellae, irradiance, glutathione (GSH), corals, Fungia
Exploited but Unevaluated: DNA Barcoding Reveals Skates and Stingrays (Chordata, Chondrichthyes) Species Landed in the Indonesian Fish Market Hawis Madduppa; Rani Utari Ayuningtyas; Beginer Subhan; Dondy Arafat; Prehadi .
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.807 KB) | DOI: 10.14710/ik.ijms.21.2.77-84

Abstract

Reliable and precise species identification is important to fisheries management and conservation. However, many rays and skates in Indonesia are currently being exploited and landed into traditional fish market without a proper identification. Therefore, this study was conducted to identify species of skates and stingrays that were landed and traded in three fish markets in Indonesia (Palabuhanratu, Muara Saban, and Lampung) using molecular techniques and to determine the conservation status of the identified species based on IUCN (International Union for Conservation of Nature and Natural Resources) as well as defined by CITES (Convention on International Trade in Endangered Species). The mitochondrial cytochrome oxidase I (COI) gene was amplified by polymerase chain reaction (PCR) using a pair of primer, fish-BCL and fish-BCH. Of 29 tissue samples collected from the study sites, a total of five species were successfully identified: Dipturus chilensis (4), Himantura walga (1), Neotrygon kuhlii (11), Taeniura lymma (9) and Rhinoptera javanica (4). The Neighbor Joining phylogeny of mitochondrial lineages, based on partial COI gene sequences, the ingroup haplotypes were clustered into five main clades representing each species. The identified stingrays were being listed as vulnerable (D. chilensis and R. javanica), near threatened (H. walga and T. lymma), and data deficient (N. kuhlii) by IUCN, with two species (D. chilensis and H. walga) population were indicated decreased. Unfortunately, all of identified species have not been evaluated by CITES regarding their trade status. As a consequences, a valuable effort should be placed to create a scientific network for monitoring programmes not only on a local scale, and to make pressure on governments for adopting molecular techniques as tools for controlling and avoiding misidentification. Keywords: Mitochondrial DNA, Phylogeny, Coral Triangle, Taxonomy, Fisheries
KARAKTERISTIK HAMBUR BALIK AKUSTIK PADA IKAN KAKAKTUA (Chlorurus sordidus) MELALUI PENGUKURAN SECARA EX-SITU DENGAN METODE AKUSTIK Deddy Bakhtiar; Indra Jaya; Henry M Manik; Hawis H Madduppa
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 4 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.4.271-278

Abstract

Ikan kakaktua (Chlorurus sordidus) merupakan salah satu ikan herbivora pada ekosistem terumbu karang yang memiliki peran dalam menjaga kesehatan terumbu karang. Penelitian ini bertujuan untuk mempelajari karakteristik hambur balik akustik ikan kakaktua dan hubungannya dengan ukuran panjang ikan. Manfaat dari penelitian ini berguna untuk pendugaan distribusi ukuran dan kelimpahan ikan kakaktua di suatu perairan. Pengukuran hambur balik akustik dilakukan secara ex-situ menggunakan metode tethered, dimana ikan kakaktua digantung pada kedalaman 2 meter di bawah transduser dan di sounding dengan echosounder Simrad EK-15 pada frekuensi 200 kHz. Data hasil perekaman dianalisis dengan software Echoview 8 untuk memperoleh data target strength (TS). Hasil penelitian menunjukkan bahwa hambur balik ikan kakaktua (C. sordidus) memiliki nilai TS rata-rata -49.42 dB pada ukuran panjang baku berkisar 14 sampai 22 cm. Perbedaan nilai TS pada ikan kakaktua diduga dipengaruhi ukuran panjang tubuh, panjang gelembung renang dan aktifitas renang.  Rasio ukuran panjang gelembung renang dan panjang tubuh tidak menunjukkan keterkaitannya dengan nilai TS. Hubungan antara TS dan panjang baku ikan kakaktua (C. sordidus) diformulasikan dalam persamaan TS = 10,43 Log SL – 62,65 (R2 = 0,752). Hasil ini berbeda dengan ketetapan Love (1977) dimana hambur balik akustik dari ikan merupakan kuadrat dari ukuran panjang ikan yang tetapkan dengan nilai slope (b) sebesar 20. Parrot fish (Chlorurus sordidus) is one of the herbivorous fish in coral reef ecosystems that has a role in maintaining the health of coral reefs. This study aims to study the characteristics of acoustic backscattering of parrot fish and their relationship to fish length measurements. The benefits of this study are useful for estimating the size and abundance distribution of parrot fish in a waters. Acoustic backscattering measurements were carried out ex-situ using the tethered method, where the parrot fish was hung at a depth of 2 meters below the transducer and sounded with a Simrad EK-15 echosounder at a frequency of 200 kHz. Recording data were analyzed with Echoview 8 software to obtain target strength (TS) data. The results showed that the backscatter of parrot fish (C. sordidus) had an average TS value of -49.42 dB at standard lengths ranging from 14 to 22 cm. Differences in TS values in parrot fish are thought to be influenced by body length, swimbladder length and swimming activity. The ratio of the size of the swimbladder length and body length does not indicate an association with TS values. The relationship between TS and standard length of parrot fish (C. sordidus) was formulated in the equation TS = 10.43 Log SL - 62.65 (R2 = 0.752). This result is different from the Love (1977) provision where the acoustic backscatter of a fish is the square of the length of the fish set with a slope (b) of 20. 
IDENTIFICATION OF GROUPER (EPINEPHELUS SP) AT MUARA ANGKE TRADITIONAL FISH MARKET IN NORTH JAKARTA USING MORPHOLOGY AND DNA BARCODING METHODS Shodikin Aznardi; Hawis Madduppa
Berkala Perikanan Terubuk Vol 48, No 1 (2020): Februari 2020
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253 KB) | DOI: 10.31258/terubuk.48.1.298-303

Abstract

Grouper fish is the most exploited commodity for coral fisheries. The controversy that occurred between researchers regarding the identification of fish species is a problem that is quite alarming. Identification using morphometric measurements, shape and color or morphology is considered still not accurate in determining the fish species. The DNA barcoding method uses COI markers by tracing the nucleotide bases to determine which species of biota have advantages in species identification. This study aims to identify grouper fish species in the traditional Muara angke fish market by using morphological methods and DNA barcoding. The identification results obtained from the morphological methods (shape, color and morphometrics) that the sample species are white-tailed grouper fish (Epinephelus areolatus) by looking at morphological characteristics, namely the white lines on the caudal fins and brown spots on the body. Identification using the DNA Barcoding method using the COI markers showed that the fish samples were 95% similar to E. areolatus.
IDENTIFICATION OF SPECIES Carangoides malabaricus USING MORPHOLOGICAL ANALYSIS AND DNA BARCODING FROM MUARA ANGKE, NORTH JAKARTA Novian Prahandhy Kusuma; Hawis Madduppa
Berkala Perikanan Terubuk Vol 48, No 1 (2020): Februari 2020
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.496 KB) | DOI: 10.31258/terubuk.48.1.304-308

Abstract

Keseimbangan suatu ekosistem tercipta oleh adanya interaksi antara makhluk hidup dengan lingkungannya. Rusak atau hilangnya suatu aspek akan mengganggu keseimbangan ekosistem tersebut. Salah satu aspek biota yang memiliki peranan dalam ekosistem adalah ikan Carangoides malabaricus yang merupakan konsumen perairan.  Penelitian ini bertujuan untuk mengidentifikasi sampel ikan menggunakan analisis morfologi dan DNA barcoding. Hasil analisis menggunakan morfologi menunjukkan bahwa sampel yang ada berasal dari genus Carangoides. Hasil analisis menggunakan COI menunjukkan bahwa sampel merupakan ikan Carangoides malabaricus dengan nilai kesamaan mencapai 99,40%
Identifikasi Caesio cuning berdasarkan Karakterisasi Morfometrik dan DNA Barcoding yang didaratkan di Pasar Ikan Muara Baru, Jakarta Muhammad Fahmi Zuhdi; Hawis Madduppa
Jurnal Kelautan Tropis Vol 23, No 2 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i2.7036

Abstract

Yellow-tailed fish (Caesio cuning) have morphologically similarities with Lutjanidae families, it causes ambiguity on species authentication process. The process of species identification using morphological characteristic does not provide a precise information related to the species.This study was aimed to identify the morphometric and molecular of Yellow-tailed fish (Caesio cuning) which landed on Muara Baru Fish Market, Jakarta using COI gene. A total 30 fishes were observed their nineteen morphometric characters, and 1 fish sample was taken from the fins for DNA extraction, amplification using PCR method, electrphoresis visualization, and sequensing. Sample was analyzed by MEGA 6 software. Based on morphological analysis showed that sampel are Yellow-tailed fish which part of Caesio genus and Caesionidae family. While, genetic analysis using COI gene showed has similarities with database of Genbank NCBI. It can be concluded that identification using morphological character and DNA barcoding methode showed the species belong to Yellow-tailed fish (Caesio cunning).  Ikan Ekor Kuning (Caesio cuning) memiliki kemiripan morfologi dengan anggota famili Lutjanidae lainnya, hal tersebut menyebabkan kesulitan dalam proses autentikasinya.  Proses identifikasi pada suatu spesies menggunakan karakteristik morfologinya belum mampu memberikan informasi yang akurat terkait spesies tersebut. Penelitian ini bertujuan untuk mengidentifikasi ikan ekor kuning (Caesio cuning) yang didaratkan di Pasar Muara Baru, Jakarta melalui kajian karakteristikmorfometrik dan DNA barcoding menggunakan marka gen COI. Total 30 ikan diamati karakter morfometriknya, dan 1 sampel ikan diambil bagian siripnya untuk dilakukan ekstraksi DNA, amplfikasi PCR, elektroforesis, sekuensing dan dianalisis menggunakan aplikasi MEGA 6. Hasil analisis morfologi menunjukkan sampel ikan berasal dari genus Caesio dan termasuk famili Caesionidae. Sedangkan berdasarkan analisis secara molekuler menggunakan marka gen COI, didapatkan hasil bahwa spesies yang diamati (Caesio cuning) memiliki kemiripan dengan database GenBank NCBI. Dapat simpulkan bahwa identifikasi secara morfologi dan DNA menunjukkan bahwa spesies yang di peroleh yaitu Ikan ekor kuning (Caesio cuning).
Variasi Temporal Kelompok Ikan Terumbu Karang di Pulau Tidung Kecil Menggunakan eDNA Metabarkoding dan Sensus Visual Muhammad Fahmi Zuhdi; Hawis Madduppa; Neviaty P. Zamani
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.11810

Abstract

Coral reef fish are play key role in coral reef ecosystem. The presence of reef fish affected by antrophogenic and natural factors, such seasonal changes. This study aimed to asess the temporal variation of coral reef fish group in Tidung Kecil Island using eDNA metabarcoding and Undewater Visual Census. This research was conducted at December 2019 (West season) and August 2020 (East season). Target group are dominated in west season (64.1%) and east season (59.25%) using eDNA metabarcoding. While, major group fish are the highest relative abundance in both season by using Underwater Visual Census. Family Carangidae are the highest species richness (15 species) in wet season and Serranidae (3 species) in east season, respectively.  Futhermore, famili Pomacentridae are the most richness species in west and east seasons 10 and 11 species respectively. Thus, it can be concluded these two methods are effective for monitoring structure or abundance of coral reef fish based on seasonal variation. Ikan karang menjadi indikator dalam menilai keanekaragaman hayati di ekosistem tersebut. Keberadaan ikan di ekosistem terumbu karang dapat dipengaruhi oleh faktor antropogenik dan faktor alam salah satunya perubahan musim. Penelitian ini bertujuan untuk menganalisis kelimpahan kelompok ikan terumbu karang di Pulau Tidung Kecil menggunakan eDNA metabarkoding dan Sensus Visual. Pengambilan data dilakukan pada bulan Desember 2019 (musim barat) dan Agustus 2020 (musim timur). Ikan target mendominasi pada musim barat dan timur dengan persentase sebesar 64.11% dan 59.25%. Sensus visual berhasil mendeteksi ikan mayor dengan persentase tertinggi 62.5% di musim barat dan 82.8% di musim timur. Famili Carangidae merupakan famili dengan jumlah spesies tertinggi di musim barat (15 species) dan Siganidae di musim timur menggunakan eDNA metabarkoding (3 species). Hasil UVC menunjukkan famili Pomcentridae memilki jumlah spesies tertinggi di kedua musim (11 dan 10 spesies) menggundakan sensus visual. Dapat disimpulkan bahwa kedua metode tersebut dapat menjadi pendekatan dalam monitoring struktur atau kelimpahan ikan terumbu karang berdasarkan musim. 
Identifikasi Scatophagus argus yang dipasarkan di Jakarta berdasarkan Analisis Morfologi dan DNA Barcoding Sulistiowati Sulistiowati; Hawis Madduppa
Jurnal Kelautan Tropis Vol 23, No 3 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i3.7057

Abstract

Species list of economical fish landed in Muara Baru Modern Fish Market, Jakarta needs to be examined. Fish species information needs to know the species of fish being traded. However, many species are difficult to identify morphologically. The aim of this research was to identify fish based on their morphological characters and DNA barcoding. The methods of this research were morphologic, morphometric, and molecular identification with DNA barcoding using PCR. Morphological analysis results showed that fish samples have unique characteristics in the presence of spots on their bodies and fading in the abdomen. Morphometric observations were made with 19 different characters and weight measurements. The 19 fish characters observed have a standard deviation of <1, mean that the fish samples taken have a size that was not much different. A comparison ratio of 18 morphometric characters to total length (PT) showed variable results. Genetic analysis of the fish studied had the Max Score and Total Score was same, 1201, Query Coverage was 95%, and Ident was 100%. Based on morphological analysis and DNA barcoding used by fish species identified as Scatophagus argus species. Both methods were successfully carried out and the two methods complement each other to identify fish species correctly and accurately. Jumlah ikan ekonomis yang banyak diperjualbelikan di Pasar Muara Baru, Jakarta perlu dilakukan penelitian. Informasi spesies ikan perlu diketahui untuk mengetahui jenis ikan yang diperjualbelikan. Penelitian ini bertujuan untuk mengidentifikasi morfologi ikan dengan pendekatan studi morfometrik dan DNA barcoding. Metode yang digunakan dalam penelitian ini yaitu pengamatan morfologi dan pengukuran morfometri serta identifikasi molekuler dengan DNA barcoding menggunakan PCR. Hasil analisis morfologi menunjukkan bahwa sampel ikan memiliki karakteristik yang unik dengan adanya corak totol di seluruh tubuhnya dan memudar di bagian perut. Pengamatan morfometrik dilakukan dengan 19 karakter yang berbeda serta pengukuran berat. 19 karakter ikan yang diamati memiliki standar deviasi yang <1, artinya sampel ikan yang terambil memiliki ukuran yang tidak jauh berbeda. Rasio perbandingan 18 karakter morfometri terhadap panjang total (PT) menunjukkan hasil yang bervariasi. Analisis genetik dari ikan  yang diteliti memiliki Max Score dan Total Score sama yaitu 1201 dengan Query Coverage 95%, dan Ident 100%. Berdasarkan analisis morfologi dan DNA barcoding yang digunakan spesies ikan teridenfikasi spesies Scatophagus argus.  Kedua metode tersebut berhasil dilakukan dan kedua metode tersebut saling melengkapi untuk melakukan identifikasi spesies ikan secara tepat dan akurat.
Co-Authors . Zairion AA Sudharmawan, AA Abdul Kadir Yamko Abdurrachman Baksir Abdurrachman Baksir Aditya Bramandito Adriani Sunuddin Agus A Hakim Agus Atmadipoera Agus Kusnadi Agus Ramli Agus S. Atmadipoera Ahmad Eko Suprianto Ahmad Taufik Ghozali Ahmad Taufik Ghozali Alan Frendy Koropitan Ali Mashar Am Azbas Taurusman Anggraini, Nurlita Putri Aradea Bujana Kusuma Aradea Bujana Kusuma Arief Pratomo, Arief Ario Damar Astini, Lita Astuti, Anggini Fuji Audina putri Ayu Ervinia Ayu, Inna Puspa Aznardi, Shodikin Aznardi Beginer Subhan Benyamin, Azhari Bertoka Fajar S.P. Negara Bisman Nababan Borbee, Erin Budi Prabowo Dea Fauzia Lestari, Dea Fauzia Deddy Bakhtiar Deden Yusman Maulid Dedi Soedharma Dedi Soedharma Dedi Soedharma Denny Khaerudi Dharmawan, I Wayan Eka Dian Pertiwi Dietrich Geoffrey Bengen Dietrich Geoffrey Bengen Dietriech G Bengen Dietriech Geoffrey Bengen Dining Nika Alina Dondy Arafat Dony Kushardono Eko S Wibowo Ester Restiana Endang Gelis Fadel Muhammad Fadillah Rahmawati Fajar Nugroho Farhan Ramdhani Fatchiyyah, Sayyiddah Fauzan Dzulfannazhir Fauzan Dzulfannazhir Fildzah Z Hulwani Firdaut Ismail Firsta Kusuma Yudha Gelis, Ester R. E. Genadi Algadri Hadadi, Namira Hadi, Sutanto Hanami, Cindy Claudea Hari Eko Irianto HEDI INDRA JANUAR Henry Munandar Manik Husna, Alief K Ichtineza H Hardono Idham A. Nasrullah Ikbal Marus Ilyas, Turissa Pragunanti Indra Jaya Indra Jaya Irianda, Nadya Jeny Irmalita Tahir Ismail Ismail Iswandi Wahab Iswandi Wahab Jeddah Isnul Jhon Septin Maurisdo Siregar Jhoni Wahyu Adi Jonson Lumban Gaol Karizma Fahlevy Kustiariyah Tarman Lalu M Iqbal Sani Lalu M. Iqbal Sani Lalu M. Iqbal Sani Lane, Christopher Luky Adrianto Luzmi Malia Izza Mahardika Rizqi Himawan Majariana Krisanti Mala Nurilmala Mas Irfanto Meutia Samira Ismet Minsaris, La Ode Alam Moh Muhaemin Mohd Adip Setiawan Muhamad Darmawan Muhamad Gilang Arindra Putra Muhamad Gilang Arindra Putra Muhamad Iqbal Muhammad Andre Nugraha, Muhammad Andre Muhammad Aris Muhammad Fahmi Zuhdi Muhammad Fahmi Zuhdi Muhammad Taufik MUJIZAT KAWAROE Mujizat Kawaroe Mujizat Kawaroe Mujizat Kawaroe Mu’min Mu’min Nadya Cakasana Nadya Jeny Irianda NAILA KHURIL AINI Nebuchad Nezzar Akbar Nebuchadnezzar Akbar Nella Tri Agustini, Nella Tri Neviaty P Zamani Neviaty P Zamani Neviaty P. Zamani Neviaty P. Zamani Neviaty P. Zamani NEVIATY PUTRI ZAMANI Novian Prahandhy Kusuma Nurafni Nurafni Nurhaya Afifah Nurjanah Nurjanah Nurlisa Alias Butet Nurlita P Anggraini Nurlita Putri Anggraini Nurlita Putri Anggraini Prakas Santoso Prakas Santoso Prakas Santoso Prakas Santoso Prehadi . Prehadi Prehadi Purwanto, Budi Rahmat Sawalman Raismin Kotta Ramadian Bachtiar Ramili, Yunita Rani Utari Ayuningtyas Raymon Rahmanov Zedta Ridha Fathihatunnisa Rifki Aldi Ramadhani Risnita Tri Utami Risti Endriani Arhatin Rizky Janatul Magwa Romanus Edy Prabowo Rommy M. Abdullah Rosmi N Pesillette Rustam E Paembonan Ruzanna, Arina Salsabila, Afviya Samsul Bahri Samsul Bahri Sani, L. Mukhsin Iqbal Sani, Lalu M Iqbal Sani, Lalu Mukhsin Iqbal Satya Pratama Atmaja Sayyidah Fatchiyyah Sayyiddah Fatchiyyah Sebastian C. A. Ferse Sebastian Ferse Septy Heltria Setyaningsih, Wahyu Adi Shita Azaraly, Irma Shodikin Aznardi Sigid Hariyadi Sihono Sihono SITI KHODIJAH Sulistiono Sulistiowati Sulistiowati Surahman Surahman Sutanto Hadi Syamsul Arifin Syarifudin Nur Taqiyuddin, Muhammad Wijdan Teddy Triandiza Teddy Triandiza Tyani Fitrian Udhi E Hernawan Ummu Salma Wedi Andika Wiwid Andriyani Lestariningsih Yoppie Wulanda Yusli Wardiatno Zakiyah Rahim Zedta, Raymon Rahmanov