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All Journal Rotasi J@TI (TEKNIK INDUSTRI) Jurnal Ilmu Lingkungan Industrial Engineering Online Journal Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Sinergi Jurnal Ilmiah Teknik Industri MIX : Jurnal Ilmiah Manajemen Jurnal Sistem dan Manajemen Industri Jurnal Teknik Industri: Jurnal Keilmuan dan Aplikasi Teknik Industri Indonesian Food Science and TechnologyJournal ComTech: Computer, Mathematics and Engineering Applications STI Policy and Management Journal Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Advance Sustainable Science, Engineering and Technology (ASSET) Food Scientia: Journal of Food Science and Technology Makara Journal of Technology Jurnal Agrifoodtech Journal Community Service Consortium Jurnal Profesi Insinyur Indonesia
Ratna Purwaningsih
Departemen Teknik Industri Fakultas Teknik - Universitas Diponegoro
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Social Sciences Transportation

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Journal : Indonesian Food Science and TechnologyJournal

 1 
Detection of Lysteria monocytogenes in Frozen Meatballs Using Real-Time PCR Sophian, Alfi; Purwaningsih, Ratna
Indonesian Food Science and Technology Journal Vol. 6 No. 1 (2022): Volume 6. Number 1, December 2022 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v6i1.20391

Abstract

Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meatballs is one of the critical test parameters in product quality testing. The challenge in testing product quality is how to find a reliable and effective test method to generate test results data. With this research, it is hoped that it can provide information or references for similar research. The method used in this research is the enrichment method with a direct isolation technique and amplification using real-time PCR. Data analysis was carried out based on 2 main criteria which included: (1) Ct analysis (Cycle threshold) and (2) melting temperature (Tm) analysis. From the results of the analysis using real-time PCR, the results of the tested samples were detected at Ct 11.60 with a Tm value of 81.50. For NTC control not detected, this indicates that the master mix used is in good condition and no contamination occurred when testing the sample. The use of this NTC control plays an important role in monitoring the quality of the master mic and test run. For the LOD control and positive control, the results were detected, whereas in the LOD control, the Ct value was detected at 10.08 and the Tm value was at 81.30. for positive control, the value of Ct was detected at 10.85 and the value of Tm was at 82.0. Based on the results of the study, it can be concluded that the test method used can detect Listeria monocytogenes in processed meat products in the form of frozen meatballs.
Co-Authors Adrian Hartanto, Adrian Afifah Ahmad Afif Aisyah Aisyah Aisyah Aisyah Alya Az Zahraa Aminatuzzuhra Aminatuzzuhra, Aminatuzzuhra Anam, Muhammad Syaiful Arief Sugiyanto Aries Susanty Arimi, Izzan Ary Arvianto Aulia, Yussy Ayu, Fadila Dyah Bambang Purwanggono Belan Adison Cahya Farradita Sulistya Cahyantari, Anggaina Elfandora Christine Nauli Ibrahim Della Dias Oktarianingrum Devi Amalia Ayuningtias Dien, Shane Atsya Amara Dinar Anggita Restuti Dyah Hesti Wardhani Dyah Priamsari Dzulfikar, Rifki Daris Ernan Rustiadi Erwin Ardiansyah Fadhlandi Naufan Zharfano Fadli, Dinda Anggraini Fahira, Ayu Leuditya Fauzi, Muhammad Yusufa Riyan Gita Mahdiah Gunawan, Mega Sri Handy Nugroho, Handy Hapsari, Chaterine Alvina Prima Hari Kuncara Haryo Santosa Hayyina, Hilma Heru Prastawa Hery Suliantoro Ikhsan Yenifi Khalis Riyangga Kharisma Panca Kurniawati, Kharisma Panca Kresnanggara, Dionisius Yudhit Kusumawaty, Rini Mahardhika Cakra Yudha Maulana Ihsan Wardhana Maypemi, Niari Prittu Meila Zulhiana Meriska Damayanti Muhammad Arif Widyoadi Nadira Apsari Naniek Utami H Naniek Utami Handayani Naniek Utami Handayani Novie Susanto NURUL MIRANDA, NURUL Oktaviani Hadiati Putri Oryzanti, Parwa P., Dyah Ayu Pajar Damar Kusuma Pangestu, Winda Widya Prayogi, M. Agam Prima Hazairin Pugara, Ade Purnawan Adi Wicaksono Putri Indah Ramadani Putri, Ade Aisyah Arifna Raharjo, Danu Raka Mahendra Sulistiyo Rani Rumita Restuti, Dinar Anggita Riko Agisdihan Afifi Riska Ayu Purnamasari Riza, Fhariq Aulia Rizki Ridha Illahi Setiawan, Marwan Shintyastuti, Annisa Rahma Silviana Silviana Simanjuntak, Christoper Frans Singgih Saptadi Sjarief Widjaja Sonia, Regita Sophian, Alfi Sri Gunani Partiwi Sri Hartini Sri Sumiyati Sri Utaminingsih Suci Permata Sari Sulardjaka Sulardjaka Susatyo Nugroho Susatyo Nugroho W.P. Susatyo Nugroho WP Syamsinuha, Nabiqa Theresia Sihombing Tri Listyorini Unggul Dewandana Erich Bagaskara, Unggul Dewandana Erich Utami, Ajeng Sri Vita Shany Nugrahaeni Wardah Wardah Wibowo, Wido Kusumo Widharto, Yusuf Wiguna, I Kadek Candra Parmana Wijanarko, Aji Wiwik Budiawan Yahya, Muhammad Dimas Yenifi, Ikhsan Yenita . Yudha, Mahardhika Cakra Yulfin, Muhammad Dhani Yulita Arni Priastiwi Yussy Aulia Zainal Fanani R Zainal Fanani Rosyada