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All Journal Majalah Kedokteran Bandung Althea Medical Journal Indonesian Journal of Tropical and Infectious Disease Global Medical and Health Communication ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Padjadjaran Journal of Dentistry Universa Medicina Paediatrica Indonesiana Chimica et Natura Acta Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia International Journal of Health Science and Technology Odonto dental journal

Ani Melani Maskoen

Universitas Padjadjaran

Author-ID : 3155714

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Optimized steps in determination of malondialdehyde (MDA) standards on diagnostic of lipid peroxidation Fauziah, Prima Nanda; Maskoen, Ani Melani; Yuliati, Tri; Widiarsih, Erlina
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Lipid peroxidation, one of the known indices of oxidative stress, is documented in various diseases. Secondary oxidation products such as malondialdehyde (MDA) is commonly measured to observe lipid peroxidation. In this study, a spectrophotometric method was evaluated to measure thiobarbituric acid reactive substances (TBARS) with high sensitivity. This study was aimed to optimisation standard of MDA using tetraethoxypropane (TEP) 97% (FW=220.3). Methods: The method is based upon the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. MDA is a known biomarker of oxidative status in a biological system. This research consists of two phases: first, making a stock of TEP, and the second phase was testing the concentration of TEP for finding the standard curve of MDA before used in diagnostic of lipid peroxidation. Results: Result showed the concentration 1,875-60 uM of TEP could form a precise standard curve. Conclusion: This concentration of TEP can be used as a reference as the standard of control in diagnostic of lipid peroxidation using TBARS method.

The effects of IRF6 rs2235373 polymorphism on mRNA expression changes in non-syndromic cleft lip and palate with various phenotypes Nasroen, Saskia Lenggogeni; Maskoen, Ani Melani; Soedjana, Hardisiswo; Soeria Soemantri, Eky Setiawan; Hilmanto, Dany
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Introduction: The IRF6 rs2235373 (C/T) is a polymorphism in the intron region that has been associated with non-syndromic cleft lip and palate (NS CLP) among some populations. Polymorphism in introns can also affect the transcription that should be detected through some changes in IRF6 mRNA expression. This study was aimed to evaluate the effects of IRF6 rs2235373 polymorphism on mRNA expression changes in NS CLP with its phenotypes which include complete unilateral (CU) CLP, complete bilateral (CB) CLP, cleft lip only (CLO), and cleft palate only (CPO). Methods: This study was a laboratory analytical study, using samples from 198 individuals in Indonesia (136 individuals with NS CL/P consisted of CU CLP (n=42), CB CLP (n=34), CLO (n=33), CPO (n=27), and 62 individuals as controls). DNA was extracted from venous blood, and the segment of rs2235373 was evaluated by PCR and Sanger sequencing. mRNA expressions analysis preceded by RNA extraction from oral epithelium, then real-time (RT) PCR was performed. mRNA expression changes were analysed by Livak method and Kruskal Wallis test followed by the Mann Whitney test through CC, GT, and TT genotype. Result: The mRNA expression of TT genotype from NS CU CLP and control was 3.36 higher value than CC genotype (p=0.008), and the mRNA expression of CT genotype from NS CB CLP and control was 0.16 lower value than CC genotype (p=0.000). Conclusion: The IRF6 rs2235373 polymorphism of TT genotype tend to cause mRNA overexpression in NS CU CLP, and CT genotype tend to cause mRNA underexpression in NS CB CLP.Keywords: Non-syndromic cleft lip and palate, NS CLP phenotype, IRF6, rs2235373, mRNA expression.

Predisposing factors analysis of mandibular anterior tooth crowding in the mixed dentition period by the tooth size and dental arch width Indriyanti, Ratna; Efendi, Sjarief Hidajat; Maskoen, Ani Melani; Riyanti, Eriska
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Introduction: Tooth crowding can cause complaints if not treated early — this condition resulting in aesthetic disorders, and also masticatory and speech function disruption. The study was aimed to determine the predisposing factors of tooth crowding in the mixed dentition period by the tooth size and dental arch width. Methods: Research type was random cross-sectional. The sample was as much as 29 children with mandibular casting; then the mandibular model cast of these 6 – 9-years old children were divided into two groups according to the crowding condition in the mandibular anterior region, and the measurement was performed afterwards. Space available category was (A/A1), mesiodistal width of mandibular incisor (B/B1), total arch length (C/C1), intercanine width (D/D1), first primary intermolar width (E/E1), second primary intermolar width (F/F1), first permanent intermolar width (G/G1), interalveolar width (H/H1). Result: The results of the t-test showed significant differences (p < 0.05) in the available space for 4 incisors, where the space was larger in the non-crowded group with a difference of 2.22 mm and the mesiodistal sizes of these 4 incisors was larger in the crowded teeth group, with the difference of 1.8 mm. The results of the Pearson correlation coefficient showed a positive relationship to several measurements, namely D to C1, E to D1 and H to B1, C1, D1, F1 and G1, with the t-value > 3 and the r-value was closer to 1, which showed a correlation with strong closeness degree (r > 0.61). Conclusion: Predisposing factors of tooth crowding in the mixed dentition period were the first intercanine width (D), first primary intermolar width (E), and alveolar arch width (H).Keywords: Tooth crowding, arch dimension, mixed dentition period.

In Silico Study of Brazilin from Secang Wood (Caesalpinia Sappan L) as a Candidate for Splenomegaly Therapy Youngest, Racy; Rusdianto, Rusdianto; Kamisah, Yusof; Maskoen, Ani Melani; Safitri, Ratu
International Journal of Health Science and Technology Vol. 6 No. 3 (2025): April
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/ijhst.v6i3.4032

Brazilin is a flavonoid found in secang (sappan) wood extract (Caesalpinia sappan L) currently undergoing clinical trials in phase 2 for the treatment of thalassemia patients. It is recognized for its antioxidant effects and its efficacy as a strong iron chelator, facilitating the binding and excretion of excess iron in the bloodstream of patients with thalassemia. This flavonoid compound may serve as a Janus kinase 2 (JAK2) inhibitor through the EPO/EPOR/JAK2/STAT5 pathway, which is responsible for splenomegaly (enlarged spleen). This study aims to investigate the mechanism by which sappan wood metabolite chemicals (brazilin) inhibit JAK2 in silico. This inhibition is expected to reduce splenomegaly in thalassemia patients and serve as an alternative to ruxolitinib (conventional medications). The pharmacokinetic profile of the ligand is predicted according to Lipinski's rule, while the binding energy (ΔG), initiation constant, and chemical bonds are examined using molecular docking with AutoDock v.4.25. This study successfully determined that brazilin, with a binding energy of -8.37 kcal/mol, is comparable to ruxolitinib, which has a binding energy of -8.71 kcal/mol. This finding shows that brazilin derived from sappan wood contains bioactive chemicals with potential JAK2 inhibitory activities. This finding establishes a foundation for further research aimed at developing new therapeutic agents for the treatment of splenomegaly in β-thalassemia and associated disorders.

Analysis of FOXE1 rs3758249, IRF6 rs2235375, MTRR A66G in Non-syndromic Cleft Lip and Palate among Indonesian Deutero-Malay Population Nasroen, Saskia Lenggogeni; Maskoen, Ani Melani; Rahaju, Asih; Darwis, Rudi Satria; Putri, Maulia; Rachman, Levina Azzahra; Zannah, Putry Nurul
Global Medical & Health Communication (GMHC) Vol 12, No 3 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Non-syndromic cleft lip and palate (NS-CLP) is one of the most common orofacial malformations, with an incidence of 1 in 700 live births worldwide. This study aimed to determine the risk factor for NS-CLP among the Indonesian Deutero-Malay population by analyzing the FOXE1 rs3758249, IRF6 rs2235375, and MTRR A66G polymorphisms. It is a case-control study, using 50 samples of NS-CLP patients and 50 samples of control (for FOXE1 rs3758249 and MTRR A66G), 30 samples of NS-CLP patients, and 30 samples of control (for IRF6 rs2235375). After DNA was extracted, polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) were performed by using restriction enzymes of Mscl (FOXE1 rs3758249) and Nde1 (MTRR A66G), and sequencing was performed for IRF6 rs2235375. The study was done in the Molecular Genetic Laboratory, Faculty of Dentistry Universitas Padjadjaran Bandung, from September 2023 to January 2024. The chi-square test was used with the exact Fisher's alternatives. The results showed that in FOXE1 3758249, A allele (mutant) was found more in control (OR=0.744, p>0.05), in MTRR A66G, G allele (mutant) was found more in NS-CLP (OR=1.267, p>0.05) meanwhile in IRF6 rs2235375, G allele (mutant) (OR=1.710, p>0.05) was found more in NS-CLP. This study concluded that FOXE1 rs 3758249, IRF6 rs2235375, and MTRR A66G genes were not the risk factor for NS-CLP in the Indonesian Deutero-Malay population. DOI: https://doi.org/10.29313/gmhc.v12i3.14110

Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November 2009
Publisher : Universitas Padjadjaran

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.

Pengetahuan Tentang Talasemia pada Mahasiswa Kedokteran dan Dokter Umum di Bandung serta Prevalensi Karir β-Thalassemia Sahiratmadja, Edhyana; Wijaya, Michelle Angelica; Widjajakusuma, Anggraini; Maskoen, Ani Melani; Dewantoro, Julius Broto; Panigoro, Ramdan
Majalah Kedokteran Indonesia Vol 70 No 4 (2020): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.70.4-2020-232

Background: Thalassemia major ranks 5th among catastrophic diseases according to the National Health Insurance, BPJS. Health workers are expected to have good knowledge about thalassemia to raise public awareness for thalassemia carrier early detection. Aim: To explore the knowledge about thalassemia among medical students and general practitioners (GPs) in Bandung, and the prevalence of β-thalassemia carrier.Methods: Analytic study was designed using questionnaire to compare knowledge. The prevalence of anemia due to iron deficiency or β-thalassemia carrier was described using Mentzer Index (MCV/RBC) and Shine and Lal index (MCV*MCV*MCH/100). Results: The knowledge about thalassemia among medical students (n597) and GPs (n171) was moderate, however, there was a significant difference; GPs greater than 30 years old had lower knowledge compared to students. Students attended blood examination had better knowledge (p 0.036). Type of anemia among students (7.9%) was different according to Mentzer index vs. Shine and Lal index; iron deficiciency anemia (5.3% % vs. 3.2%) dan β-thalassemia carrier (2.6% vs. 4.7%). Conclusions: Knowledge of thalassemia among medical students and GPs need to be enhanced, since 6-10% of Indonesian population are thalassemia carriers. Further examination is needed to confirm thalassemia carriership.

Co-Authors Afandi Charles, Afandi Agnes Rengga Indrati Alvinsyah Adhityo Pramono Anggraini Widjajakusuma, Anggraini Anugrah Aulia Ulil Amri, Anugrah Aulia Ulil Artilia, Ira Atia Nurul Sidiqa Attamimi, Fathimah Azzahra Bethy S. Hernowo Budi Setiabudiawan Choirunnisa, Tias Cissy B. Kartasasmita Cynthia Kurniawan Dany Hilmanto Darwis, Rudi Satria Dewantoro, Julius Broto Dias Aryani Edhyana Sahiratmadja Edhyana Sahiratmadja Edhyana Sahiratmadja Edhyana Sahiratmadja Edhyana Sahiratmadja Efendi, Sjarief Hidajat Eky Setiawan Soeria Soemantri Eriska Riyanti Fitri Rahmi Fadhilah Florence Meliawaty Hardisiswo Soedjana Hendro Hendro Herman Susanto Herman Susanto Herry Garna I Made Joni Ida Parwati Indah Puti Rahmayani S. Kamisah, Yusof Khan, Muhammad Salman Lelani Reniarti Lelani Reniarti Maulia Putri, Maulia Mohammad Ghozali Muqdas, Faza Annisa Myrna Nurlatifah Zakaria, Myrna Nurlatifah Nasroen, Saskia Lenggogeni Nita Susanti Nurul Setia Rahayu Penggoam, Simeon Prasdiaz, Putri Indriani Prima Nanda Fauziah R. Agus Nurwiadh Rachman, Levina Azzahra Rahaju, Asih Rahayu, Nurul Setia Ramdan Panigoro Ratna Indriyanti, Ratna Ratu Safitri Reni Ghrahani Rovina Ruslami, Rovina Rusdianto Rusdianto, Rusdianto Samsudin Surialaga Sari Puspa Dewi Sarilita, Erli Saskia L. Nasroen, Saskia L. Simeon Penggoam Sulaksono, Haura Labibah Salsabil Sumantri, Nur Imaniati Syarief Hidayat Tichvy Tammama, Tichvy Tri Hanggono Achmad Ullah, Irfan Widiarsih, Erlina Widiarsih, Erlina Wijaya, Michelle Angelica Youngest, Racy Yuliati, Tri Yuliati, Tri Zannah, Putry Nurul

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