Rika Meliansyah
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran Jalan Raya Bandung-Sumedang KM 21. Jatinangor, Indonesia 45363

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Journal : Cropsaver : Journal of Plant Protection

Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Paramita, Hedi; Puspasari, Lindung Tri; Maharani, Rani; Supratman, Unang; Hidayat, Yusup; Meliansyah, Rika; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Hedi Paramita; Lindung Tri Puspasari; Yusup Hidayat; Rika Meliansyah; Danar Dono; Rani Maharani; Unang Supratman
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.565 KB) | DOI: 10.24198/cs.v1i1.16995

Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Bioactivity Fraction of Methanolic Seed Extract of Barringtonia asiatica L. (Kurz.) (Lecythidaceae) Against Spodoptera litura F. (Lepidoptera: Noctuidae) Teddy Budiyansyah; Danar Dono; Rika Meliansyah; Unang Supratman
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.142 KB) | DOI: 10.24198/cs.v1i2.19755

Abstract

Water seed extract of Barringtonia asiatica was used as fish poison by traditional community. This study aims to determine the bioactivity of methanolic seed extract fraction of B. asiatica against S. litura. The extract tested was spread evenly on the leaf surface by using microsyringe. The experiment consisted of 12 treatments and 3 replications. Treatments were concentration of 0.3% and 0.1% of fraction 16, 17, 18, 19, 20 of methanolic seed extract of B. asiatica, and concentration of 0.264% methanolic seed extract of B. asiatica; and control. The results showed that the fraction 19 (20% EtOAc and 80% MeOH eluent) of methanol extract of B. asiatica seed has the highest bioactivity against the larvae of S. litura. The fraction 19 of methanolic seed extract of B. asiatica had LC50 value of 0.182% at 12 days after treatment. In addition, the 19 fraction of methanolic seed extract of B. asiatica decreased food intake, weight and lengthened development time of larvae.Keywords : Barringtonia asiatica, bioactivity, fraction, Spodoptera litura.
Effect of Neem Oil Formulation on the Population of Soil Fungi and Disease Intencity of Cercospora Leaf Spot (Cercospora capsici) on Chilli Plants (Capsicum annuum) Sri Hartati; Danar Dono; Rika Meliansyah; Mukhamad Agung Yusuf
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.176 KB) | DOI: 10.24198/cs.v1i2.19753

Abstract

Soil fungi and cercospora leaf spot are soil inhabitants and plant disease, respectively, that usually occur on chilli grown area. Formulation of neem oil was reported to have a role as an environmentally friendly botanical pesticide. This research was objected to studying the effect of neem oil formulation on the population of soil fungi and its suppression on cercospora leaf spot intensity of chilli crop. The field experiment was arranged in the randomized complete block design. The treatments were the concentration of neem oil formulation, i.e. 0%, 1%, 1.25%, 1.50%, 1.75%, and 2.0%. In vitro experiment on the suppression of neem oil formulation on the predominant soil, fungi were arranged in the completely randomized design. The results showed that neem oil formulation did not affect the soil fungi population and cercospora leaf spot intensity. However,2.0% neem oil had the lowest soil population. Neem oil formulation at 1.75% caused the highest suppression on TMa01 isolate (49.81%), and 1.25%, 1.50%, 1.75% and 2.0% of neem oil formulation caused more than 50% suppression on TMf01 isolate in vitro. Neem oil formulation at 2.0% caused the highest suppression on cercospora leaf spot intensity (46.34%).Keywords: Azadirachta indica, botanical pesticide, neem oil
The Ability of Three Species of Yeast in Inhibiting the in vitro Growth of Sclerotium rolfsii Sacc., the cause of damping off on soybean plants (Glycine max L.) Hartati, Sri; Setiani, Cahya; Meliansyah, Rika; Yulia, Endah; Mayanti, Tri
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58059

Abstract

Damping off disease caused by Sclerotium rolfsii is one of the important diseases of soybean plants. Biocontrol is considered as more environmentally friendly control method. Yeast is one of the biocontrol agents that can be used to control plant pathogens. This study was objected to test the potential of three species of yeast in inhibiting the growth of S. rolfsii. The experiment was arranged in the completely randomized design. The treatments were dual culture of the pathogen vs the yeasts on PDA and double dishes of S. rolfsii against three yeast isolates i.e. Rhodotorula minuta Dmg 16 BEP, Candida tropicalis Lm 13 BE, Aureobasidium pullulans Dmg 11 DEP, and a control. The results of the dual culture antagonism test showed that the three tested yeasts could inhibit the colony diameter of S. rolfsii by 23.30% – 40.00%, and the sclerotia formation by 46,33% – 98,05%. The results of the double dishes antagonism test showed that the three tested yeast isolates could inhibit the colony diameter of S. rolfsii by 19.60% – 28.20% and could inhibit 100% of sclerotia formation. The treatment of A. pullulans Dmg 11 DEP produced the highest inhibition in both the dual culture and double dishes antagonism tests.
The Ability of Nano Chitosan and Bacillus subtilis (Ehrenberg) Cohn to Suppress the Growth of Alternaria porri (Ellis) Ciferri Al Farisy, Muhammad Restu; Meliansyah, Rika; Susanto, Agus; Hersanti, Hersanti
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58185

Abstract

Purple blotch disease, caused by the fungus Alternaria porri (Ellis) Ciferri, poses a significant threat to shallot crops. Environmentally friendly control methods, such as applying nano-sized chitosan and Bacillus subtilis (Ehrenberg) Cohn, offer promising alternatives. This study aims to determine the effective concentrations of nano chitosan and B. subtilis, individually and in combination, to suppress the growth of A. porri in-vitro. The research was conducted from March to June 2024 at the Phytopathology Laboratory and Plant Protection Biotechnology Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Universitas Padjadjaran. A completely randomized design was employed, consisting of nine treatments with three replications each: a control treatment, B. subtilis 107 CFU/ml, 50 ppm nano chitosan, 100 ppm nano chitosan, 200 ppm nano chitosan, B. subtilis 107 CFU/ml + 50 ppm nano chitosan, B. subtilis 107 CFU/ml + 100 ppm nano chitosan, B. subtilis 107 CFU/ml + 200 ppm nano chitosan, and 80% mancozeb. The results indicated that nano chitosan at a concentration of 100 ppm was the most effective single treatment which could suppress the growth of A. porri colonies by 94% and inhibit conidia germination by 95%. Meanwhile, B. subtilis 107 CFU/ml + 100 ppm nano chitosan is the most effective mixed treatment which could suppress the growth of A. porri colonies by 84% and inhibit conidia germination by 90%.
Toxicity Effect of A Mixture Spiked Pepper (Piper aduncum L.) And Castor Bean (Ricinus communis L.) Against Crocidolomia pavonana F. Narendra, Nathanael Dimas; Dono, Danar; Meliansyah, Rika
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58910

Abstract

Crocidolomia pavonana is a cabbage crops pest that cause huge losses for farmers. Spiked pepper (Piper aduncum L.) and castor bean (Ricinus communis L.) extracts are known to be used as botanical insecticides because they have toxic compounds. This study aims to evaluate the toxic effects of a mixture of spiked pepper extract and castor oil as plant based insecticides against C. pavonana larvae and assess the index combination. The study consists of toxicity tests using experimental methods with a Randomized Complete Block Design with six treatments and four replications which were analyzed using analysis of probit regression and followed by an analysis of the mixture properties. Toxicity tests were conducted using a single spiked pepper extract, a single castor oil, and a mixture of spiked pepper extract and castor oil. The concentration of single spiked pepper extract used was 0.15%; 0.27%; 0.51%; 0.94%; 1.75%; and control, then the concentration of castor oil was 0.15%; 0.25%; 0.43%; 0.73%; 1.25%; and control, then the concentration of mixture of spiked pepper extract with castor oil was 0.30%; 0.41%; 0.57%; 0.79%; 1.1%; and control with a ratio 1:1. The results of the toxicity test showed that the mixture of spiked pepper extract with castor oil was toxic with LC50 and LC95 of 0.468% and 1.364% respectively at 10 days after treatment. The results of the mixture properties analysis showed that the mixture between spiked pepper and castor oil was additive at LC50 with a Combination Index value of 0.975 and was weakly synergistic at LC95 with a Combination Index value of 0.524.