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Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Ferencia Esananda Rattu; Revolson Mege; Nonny Manampiring; Verawati Ida Yani Roring; Sukmarayu Gedoan; Yermia Mokosuli
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sample’s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.

ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

Peningkatan Kapasitas Produksi dan Manajemen Kelompok Tani Singkanaung melalui Pendampingan Teknologi Tepat Guna di Desa Mokoditek, Bolaang Mongondow Utara Montolalu, Friska; Rumondor, Fadly; Potalangi, Nerni; Mege, Revolson; Soputan, Grace; Mokosuli, Yeremia; Pomalingo, Moh Fikri
Servitium Smart Journal Vol 3 No 2 (2025): Servitium Smart Journal
Publisher : Universitas Klabat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31154/servitium.v4i1.35

Mokoditek Village in North Bolaang Mongondow has great agricultural potential, especially in corn and rice production. However, low productivity and limited technology have caused local farmer groups, especially the Singkanaung Farmers Group, to be unable to optimize their agricultural output. This research aims to increase the production capacity and management of farmer groups through the application of appropriate technology and intensive training. The results of the program showed an increase in corn productivity from 3 tons/ha to 4 tons/ha. In addition, financial management training has strengthened the capacity of farmer group members to manage agricultural businesses more efficiently and sustainably.

Biodegradasi Bioplastik Berbahan Dasar Pati Daluga (Cyrtosperma merkusii) dengan Cellulose Nano Crystal sebagai Agen Reinforcement sebagai Dasar Pengembangan Food Packaging Timbuleng, Nobel; Naharia, Orbanus; Gedoan, Sukmarayu Piter; Mokosuli, Yermia Semuel; Rahardiyan, Dino; Moko, Emma Mauren
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 3 (2023): Oktober
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i3.67917

Daluga (Cyrtosperma merkusii) adalah tanaman pesisir memiliki karakter sebagai tanaman paludikultur. Tanaman ini merupakan tanaman yang kurang dimanfaatkan sehingga cocok sebagai bahan utama bioplastik yang mana tidak berkompetisi dengan bahan pati pangan lainnya. Penelitian ini merupakan penelitian deskriptif kualitatif memberikan gambaran laju biodegradabilitas dalam tanah dan dalam consortium bakteri EM4 dari bioplastik daluga dengan plasticizer gliserol, subjek penelitian adalah bioplastik dari pati umbi daluga dengan gliserol sebagai plasticizer dibandingkan dengan bioplastik pati umbi daluga yang diperkuat strukturnya dengan selulosa nano kristal (CNC). Proses pengumpulan datanya dilakukan selama masa inkubasi tujuh hari, terhadap densitas, laju biodegradibilitas dalam tanah dan dalam larutan EM4, daya serap air, porositas, dan laju transmisi uap air, dimana dalam kurun waktu tersebut laju biodegradabilitasnya meningkat terutama bagi bioplastik dalam media tanah demikian juga bioplastik dalam EM4. Penambahan filler CNC memberikan nilai reinforcement bagi struktur bioplastik sehingga memperkuat struktur agar dapat dipergunakan sebagai kemasan bahan pangan dengan kadar air tinggi.. Laju biodegradibilitas bioplastik daluga yang diperkuat oleh CNC lebih lambat baik yang dalam tanah maupun yang ada dalam larutan EM4. Keterbaruan dari penelitian ini adalah pengujian biodegradibilitas dari bioplastik yang menggunakan sumber pati underutilized berbasis kearifan lokal yang diperkuat (reinforced) strukturnya dengan CNC asal limbah pengolahan pati daluga.

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