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IDENTIFICATION AND CHARACTERIZATION OF FEED PLANTS FOR Apis dorsata Binghami IN THE PINABETENGAN FOREST, MINAHASA Feydina Tewu; Orbanus Naharia; Yermia Mokosuli
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6023

Abstract

Apis Dorsata Binghami is a honey bee that is often found in the forest, which is conserved by the community, but there are also honey bees that are just left alone; the community preserves these honey bees because their honey can be traded and can also be used as medicine. This study aims to determine the plants that are a feed source for Apis Dorsata Binghami in the Pinabetengan Forest, Minahasa Regency. This research was conducted in June-August 2022. The data collected came from field survey data using descriptive methods and identified plant species with values of Reactive Density (KR), Relative Frequency (FR), Relative Dominance (DR), and Index Significant Value (INP). Plant diversity as a food source for Apis Dorsata Binghami has different types of plants based on their habitus or the characteristics of the plants themselves.
Bioactivity of Papua Red Fruit Extract (Pandanus conoideus L.) Against Superoxide dismutase, Malondialdehyde and Blood Glucose of Rat (Rattus norvegicus L.) Hyperglycemia Mokosuli, Yermia Semuel; Migau, Natalina; Wurarah, Masje
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9029

Abstract

Red fruit (Pandanus conodus L.) is a plant native to Papua, ethnomedical used as a traditional medicine with a wide spectrum of pharmacological effects. However, research on the bioactivity of red fruit is still very poorly reported. The aim of this study was to characterize the bioactive content, antioxidant bioactivity and the effect of lowering blood glucose in hyperglycemic rats. Red fruit has extracted used the maceration method with 95% ethanol. Analysis of active compound content using Gas Chromatography Mass Spectrophotometry (GC MS). Analysis of Malondialdehyde (MDA), Superoxide dismutase (SOD) and blood glucose levels was carried out using the in vivo method using white rats. The research results showed that red fruit ethanol extract contained 173 compounds from six main groups of compounds. Red fruit extract has effective in reducing MDA levels in hyperglycemic mice. K4 and K5 treatments were the best treatments for reducing MDA. Red fruit extract affects the duration of induction of increased SOD in hyperglycemic rats. Treatments K4 and K5 were the best treatments for SOD induction. Red fruit extract affects reducing blood glucose in hyperglycemic mice. Red fruit extract has antioxidant activity and lowers blood glucose. Red fruit extract has the potential to be developed as a source of bioactive antioxidants and antidiabetics. Keywords: Glucose, hyperglycemia, malondialdehyde, superoxide dismutase
Penerapan Model Pembelajaran Project Based Learning untuk Meningkatkan Hasil Belajar Biologi Peserta Didik Kelas XII di SMA Negeri 1 Kabaruan Mundaeng, Camel Gantonio; Mege, Revolson A.; Mokosuli, Yermia S.
Jurnal Pendidikan Tambusai Vol. 7 No. 3 (2023): Desember 2023
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v7i3.10724

Abstract

Model Pembelajaran Project Based Learning ialah Model Pembelajaran yang berpusat pada kegiatan pembuatan proyek yang dilakukan oleh peserta didik. Model Pembelajaran ini dapat membantu Peserta didik untuk lebih memahami materi yang diajarkan di kelas dengan melakukan kegiatan praktik secara langsung di kehidupan nyata. Penelitian yang dilakukan peneliti ini bertujuan agar peserta didik mampu mencapai ketuntasan dalam mata pelajaran biologi khususnya pokok bahasan pertumbuhan dan perkembangan makhluk hidup dengan menerapkan model pembelajaran PjBL. Penelitian in berjenis penelitian tindakan kelas. Penelitian ini dilakukan di SMA Negeri 1 Kabaruan. Subjek penelitian yang digunakan yaitu Peserta didik kelas XII IPA yang berjumlah 22 orang. Hasil penelitian menunjukkan presentasi ketuntasan belajar pada siklus I sebesar 5% dengan rata – rata 41 dan pada siklus II menjadi 82% dengan rata – rata 78,9. Hasil penelitian ini menunjukkan bahwa materi pembelajaran pertumbuhan dan perkembangan makhluk hidup dengan menggunakan model pembelajaran Project Based Learning dapat meningkatkan hasil belajar peserta didik dan mencapai ketuntasan di kelas XII SMA Negeri 1 Kabaruan.
Pengembangan Modul Pembelajaran Konsep Genetika dengan Filosofi Mapalus dan Pendekatan Keterampilan Metakognitif Mokosuli, Yermia Semuel
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 1 (2024)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Learning in the era of the industrial revolution 4.0 requires students to learn how to integrate concepts at all levels of organization and complexity as well as synthesize and analyze information that connects conceptual domains. A metacognitive approach will instill the skills the current generation needs to interact in society and the world of work. The aim of the research is to describe students' responses to the genetics learning module with the Mapalus philosophy approach to metacognitive skills. This research applies development research methods. Media experts' responses to the module were valid (78%), while material experts' responses were very valid (85%). The genetic concept module developed using the Mapalus local wisdom approach produced good responses from students both in terms of use and cognitive assessment results. The module also raises students' soft skills in their use, namely cooperation, leadership, the ability to divide tasks in groups and present the results of group work. The module developed is also able to improve students' metacognitive abilities. Students' ability to understand problems increased from 65 to 80 (maximum score 100). The ability to perform calculations increased from 55 to 70. The ability to make problem solving plans increased from 67 to 75. The Genetic Concepts Module with the mapalus philosophy and metacognitive approach can be applied in schools.
Revitalisasi Usaha Tani untuk Menunjang Pariwisata Super Prioritas Likupang Minahasa Utara Mantiri, Jeane; Manampiring, Noni; Semuel, Mokosuli Yermia; Mege, Revolson Alexius; Roring, Verawati Ida Yani; Pratasik, Stralen; Rumondor, Rolef
Jurnal Aplikasi dan Inovasi Iptek Vol 5 No 1 (2023): Jurnal Aplikasi dan Inovasi Iptek No. 5 Vol. 1 Oktober, 2023
Publisher : Denpasar Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52232/jasintek.v5i1.124

Abstract

Suatu kegiatan Pemberdayaan Kemitraan Masyarakat (PKM) telah dilaksanakan untuk memberdyakan masyarakat berwirausaha tani terpadu guna menunjang pariwisata pantai desa Marinsow Kecamatan Likupang Timur Minahasa Utara. Metode yang digunakan adalah kombinasi metode kombinasi metode Andragogik dan Pilot Project aplikasi teknlogi tepat guna usaha tani terintegrasi dengan mengoptimalkan lahan dan usaha tani Mitra berada persis di tepian jalan utama menuju kawasan wisata pantai Paal desa Marinsow. Berdasaran kegiatan sampai saat ini diperoleh hasil yaitu, (1) terfasilitasi upaya menghadirkan satu unit cultivator untuk revitalisasi pengolahan lahan pertanian, (2) terfasilitasi pengadaan mulsa plastic hitam perak (MPHP) dan peralatan pertanian lainnya untuk menunjang usaha budidaya tanaman pangan, (3) terfasiitasi pemasangan tower dan pengadaan satu unit tangki air untuk pemenuhan irigasi lahan pertanian saat musim kemarau, (4) terfasilitasi peningkatan pengetahuan dan keterampilan mitra dalam pengolahan lahan menggunakan cultivator, (5) terfasilitasi peningkatan pengetahuan dan keterampilan mitra memproduksi dan mengemas aneka produk kacang tanah menggunakan vacuum sealer dengan plastic vacuum emboose. (6) Terfasilitasi satu aplikasi pemasaran digital
PENGEMBANGAN ASESMEN DIAGNOSTIK BERBASIS MEDIA GOOGLE FORM DI MAN MODEL 1 MANADO Motulo, Serlin J; Mokosuli, Yeremia S; Kamagi, Decky W.
SOSCIED Vol 7 No 1 (2024): SOSCIED - Juli 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i1.768

Abstract

Serlin J. Motulo, 20 507 023. Development of a diagnostic assessment based on Google Form Media in MAN Model 1 Manado. Thesis, Department of Biology, Faculty of Mathematics, Natural and Earth Sciences, State University of Manado. Tondano. 2024. Supervisor Prof. Dr. Yermia S. Mokosuli, M.Si and Dr. Decky W. Kamagi, M.Sc. This research is motivated by teachers who did not maximize diagnostic assessments due to a lack of understanding and limited time spent creating, applying and analyzing data. This research aims to develop a diagnostic assessment that is effective, efficient, easy to perform and economical. This research is a developmental research using the ADDIE model which includes five stages, namely analysis, design, development, implementation and evaluation. According to the research results on products developed by media experts, the percentage obtained was 97.1% (very achievable). Then for the evaluation experts, the percentage obtained is 100% (very achievable). Moreover, materials experts, the percentage obtained was 98.75% (very feasible). Meanwhile, the test results for subject teachers' responses obtained a percentage of 97.3% (very feasible) and the average percentage of student responses was 91.8% (very feasible). The development of diagnostic assessments based on Google Forms materials is very suitable for carrying out assessments that can be carried out both online and offline
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Tuegeh, Reinny Silvana; Sumampouw, Herry Maurits; Tanor, Meity Neltje; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants
The Nutritional Content Found in Rhizophora Mangrove Fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa Mongdong, Priskila Indah Theresya; Mokosuli, Yermia S.; Ogi, Nova L.I.M
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4468

Abstract

Mangroves are fertile plants that dominate the coastal areas. The fruit of the Rhizophora mangrove can be used as food and beverage ingredients, containing numerous nutrients for the human body. The mangrove fruit contains carbohydrates, water, protein, fat, as well as secondary metabolites such as flavonoids, triterpenoids, tannins, and saponins. The aim of this research is to determine the nutritional content of the Rhizophora apiculata, Rhizophora mucronata, and Rhizophora stylosa mangrove fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa. This study employs a descriptive research method, where laboratory experiments were conducted using the SNI 01-2891-1992 method at the Baristand Manado laboratory. The nutritional analysis results for Rhizophora apiculata showed a water content of 62.17%, ash content of 1.09%, protein content of 0.59%, fat content of 0.79%, crude fiber content of 5.45%, and carbohydrate content of 35.36%. For Rhizophora mucronata, the analysis revealed a water content of 65.54%, ash content of 3.07%, protein content of 1.27%, fat content of 0.74%, crude fiber content of 7.52%, and carbohydrate content of 29.38%. Lastly, Rhizophora stylosa had a water content of 64.77%, ash content of 3.547%, protein content of 1.30%, fat content of 1.63%, crude fiber content of 7.14%, and carbohydrate content of 28.76%. These results indicate that mangrove fruits have numerous benefits and contain valuable nutrients and good nutritional value for the body. By preserving the mangrove ecosystem and utilizing the nutrients found in the fruits and leaves, there is an opportunity to explore their potential as alternative natural remedies
Isolation, Identification, and Antibacterial Assay of Indigenous Bacterial Isolates from Apis cerana Honeycomb Simbolon, Kristina Br; Mokosuli, Yermia; Lawalata, Helen J
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3441

Abstract

Apis cerana is a honey bee species with a cavity hive known as the eastern honey bee. Honey bee hives have Indigenous bacteria that have antimicrobial potential. Indigenous bacteria are free bacteria that can synthesize nitrogen compounds, sugars, and other bioactive substances. This study aims to isolate, identify and test the antibacterial of Indigenous bacteria isolate Apis cerana against gram-positive Staphylococus aureus and gram-negative bacteria Escherichia coli. This study used a descriptive research method to obtain the data from laboratory experiments. The study consisted of isolating Indigenous bacteria, identifying and testing the antibacterial agar diffusion method. The study results obtained seven isolates of AC 1, AC 2, AC 3, AC 4, AC 5, AC 6, AC 7 Indigenous bacteria from Apis cerana nests. AC 1 isolate has similarities with the genus Streptococcus, AC 2 isolates have similarities with the Klebsiella genus, while AC 3 isolates have similarities with the Bacillus genus, and AC 4, AC 5, AC 6 and AC 7 isolates have similarities with the Citrobacter genus. Indigenous bacterial isolates with potential antibacterial potential where the most significant inhibition against Staphylococus aureus bacteria was shown by isolate AC 1 (10.79 mm). At the same time, the smallest was found in isolate AC 7 (8.52 mm). The most significant inhibition against Escherichia coli bacteria was shown by isolate AC 1 (9.0 mm) while the smallest was found in isolate AC 3 (7.4 mm). Apis cerana nests have indigenous bacteria that have the potential to produce antibacterial substances
Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest Semuel, Mokosuli Yermia; Wurarah, Masje; Tuegeh, Reinny Silvana
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2: Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2739

Abstract

Apis Dorsata Binghami honey bee is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The research consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six isolates of fungi from the nest of Apis dorsata Binghami, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB2 and FAB3 had the best antagonist properties, while the FAB6 isolates had the weakest antagonist properties. The FAB2 isolate showed the best bacterial growth inhibition zone average for the isolates of oral bacteria and Staphylococcus aureus. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.
Co-Authors Alfonds Andrew Maramis Anice I. Tuda Anita C. C. Tengker Anita Costanci Christine Tengker Arrijani - Britney Putri Hermanus Budiarto Carolin Manuahe Dewa Nyoman Oka Dina Munawaroh Dina Rombot Edieli Zebua Edyson Maneasa Emma M Moko Esibrena Br Kemit Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Febiola Gabriela Tular Febiola Najoan Ferencia Esananda Rattu Ferni Margo Tumbel Ferny Margo Tumbel Feydina Tewu Friscila Avianti Djaila Friska Mery Montolalu Futwembun, Fransiska Gedoan, Sukmarayu Piter Grace Jenny Soputan H. M. Sumampouw Hariyadi Hariyadi Helen J Lawalata Helen J Lawalata Helen Joan Lawalata Hendra Pratama Maliangkay Herry Maurits Sumampouw Ilham Saputra Butar-butar IRIANI SETYAWATI Jacklin Stella Manopo James S. Turambi Jantje Ngangi Jantje Pelealu Jeane Mantiri Jimmy Langi Jonathan Polandos julduz ruland paus Kamagi, Decky D. W. Kandowangko, Sandy Kaunang, Eva Sherly Nonke Keysia Stephanie Tesalonica Mulder Manampiring, Noni Mansyur, Djailani Masje Wurarah Masye Wurarah Max Tulung Meike Paat Meisa Tabita Rogahang Meity Nelltje Tanor Melita Irene Gracia Kaligis Migau, Natalina Mongdong, Priskila Indah Theresya Motulo, Serlin J Mundaeng, Camel Gantonio Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Ogi, Nova L.I.M. Orbanus Naharia Orbanus Naharia Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pua, Devis Christian Rahardiyan, Dino Revolson Mege Rolef Rumondor Rudi Alexander Repi Rymond Jusuf Rumampuk Sibala, Henike Simbolon, Kristina Br Sinyo Herry Sumampouw Suddin Simandjuntak Sukmarayu Gedoan Sukmarayu P. Gedoan Sumampouw, Harry Maurits Taariwuan, Marlin Bernadet Thesia Megi Kurniawati Rettob Timbuleng, Nobel Tjiakra, Rosalina A. Tripena Mamuaya Tuegeh, Reinny Silvana Tumanduk, Yahya Y.R. Utari Satiman Verawati I Y Roring Verawati Ida Yani Roring Verra E. J. Mawitjere Wisda Nurjannah Abdullah Worang, Rina L. Wuwungan, Axel Yessie K. Kelly