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Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Abstract

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.
Mangrove Biodiversity of Bunaken National Park in Project-Based Learning to Improve Students’ Competitive Skills during the COVID-19 Pandemic Masje Wurarah; Mokosuli Yermia Semuel; Herry Maurits Sumampouw
Jurnal Pendidikan Progresif Vol 12, No 2 (2022): Jurnal Pendidikan Progresif
Publisher : FKIP Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mangrove Biodiversity of Bunaken National Park For Project-Based Learning To Improve Competitive Student Skills During The COVID 19 Pandemic. College graduates in the era of the industrial revolution 4.0 are required to have strong competitive abilities. Competitive skills can be measured from the science process skills possessed. Objectives: This study aims to obtain a competitive profile of students' skills through a project-based learning method on mangrove biodiversity in Bunaken National Park, North Sulawesi. Methods: The research subjects were students who took Sulawesi biodiversity courses in 2020/2021. Fourteen science process skills were measured using a rubric Findings: The results showed that students' mastery of science process skills was high in the Sulawesi biodiversity course. Conclusion: . The application of project-based learning to the problem of mangrove biodiversity in Bunaken National Park can induce students to develop problem-solving skills, collaboration, creativity, and literacy skills. Keywords: biodiversity mangrove, competitive skills, project-based learning.DOI: http://dx.doi.org/10.23960/jpp.v12.i2.202219
PENGEMBANGAN E-BOOK DAN PRAKTIKUM BERBASIS ANALISIS DNA LALAT BUAH ISOLAT LOKAL PADA MATERI GENETIKA DI SMA Friscila Avianti Djaila; Mokosuli Yermia Semuel; Masje Wurarah
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 1 (2025): Volume 8 No. 1 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i1.41423

Abstract

Penelitian ini bertujuan untuk mengembangkan dan menghasilkan media pembelajaran e-book dan praktikum berbasis analisis DNA lalat buah isolat lokal pada materi genetika di SMA yang layak digunakan. Penelitian ini merupakan penelitian pengembangan (Research and Development) dengan model ADDIE yang terdiri 5 tahapan, yaitu Analysis, Design, Development, Implementation, dan Evaluation. Instrumen tes pada penelitian ini adalah angket, wawancara dan dokumentasi. Lembar angket penilaian diberikan kepada subjek penelitian yaitu ahli materi, ahli media, guru mata pelajaran biologi dan siswa kelas XII di SMA Negeri 1 Pagimana. Hasil penelitian ahli materi dengan persentase 93% kriteria sangat valid, ahli media dengan persentase 96% kriteria sangat valid, guru biologi dengan persentase 97% kriteria sangat valid dan respons siswa dengan persentase 88% kriteria sangat baik. Dapat disimpulkan bahwa media e-book dan praktikum berbasis analisis DNA lalat buah isolat lokal pada materi genetika di SMA produk yang dihasilkan sangat layak untuk digunakan sebagai media pembelajaran.
ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Abstract

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.
Peningkatan Kapasitas Produksi dan Manajemen Kelompok Tani Singkanaung melalui Pendampingan Teknologi Tepat Guna di Desa Mokoditek, Bolaang Mongondow Utara Montolalu, Friska; Rumondor, Fadly; Potalangi, Nerni; Mege, Revolson; Soputan, Grace; Mokosuli, Yeremia; Pomalingo, Moh Fikri
Servitium Smart Journal Vol 3 No 2 (2025): Servitium Smart Journal
Publisher : Universitas Klabat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31154/servitium.v4i1.35

Abstract

Mokoditek Village in North Bolaang Mongondow has great agricultural potential, especially in corn and rice production. However, low productivity and limited technology have caused local farmer groups, especially the Singkanaung Farmers Group, to be unable to optimize their agricultural output. This research aims to increase the production capacity and management of farmer groups through the application of appropriate technology and intensive training. The results of the program showed an increase in corn productivity from 3 tons/ha to 4 tons/ha. In addition, financial management training has strengthened the capacity of farmer group members to manage agricultural businesses more efficiently and sustainably.
Biodegradasi Bioplastik Berbahan Dasar Pati Daluga (Cyrtosperma merkusii) dengan Cellulose Nano Crystal sebagai Agen Reinforcement sebagai Dasar Pengembangan Food Packaging Timbuleng, Nobel; Naharia, Orbanus; Gedoan, Sukmarayu Piter; Mokosuli, Yermia Semuel; Rahardiyan, Dino; Moko, Emma Mauren
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 3 (2023): Oktober
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i3.67917

Abstract

Daluga (Cyrtosperma merkusii) adalah tanaman pesisir memiliki karakter sebagai tanaman paludikultur. Tanaman ini merupakan tanaman yang kurang dimanfaatkan sehingga cocok sebagai bahan utama bioplastik yang mana tidak berkompetisi dengan bahan pati pangan lainnya. Penelitian ini merupakan penelitian deskriptif kualitatif  memberikan gambaran laju biodegradabilitas dalam tanah dan dalam consortium bakteri EM4 dari bioplastik daluga dengan plasticizer gliserol,  subjek penelitian adalah bioplastik dari pati umbi daluga dengan gliserol sebagai plasticizer dibandingkan dengan bioplastik pati umbi daluga yang diperkuat strukturnya dengan selulosa nano kristal (CNC). Proses pengumpulan datanya dilakukan selama masa inkubasi tujuh hari, terhadap densitas, laju biodegradibilitas dalam tanah dan dalam larutan EM4, daya serap air, porositas, dan laju transmisi uap air, dimana dalam kurun waktu tersebut laju biodegradabilitasnya meningkat terutama bagi bioplastik dalam media tanah demikian juga bioplastik dalam EM4. Penambahan filler CNC memberikan nilai reinforcement bagi struktur bioplastik sehingga memperkuat struktur agar dapat dipergunakan sebagai kemasan bahan pangan dengan kadar air tinggi.. Laju biodegradibilitas bioplastik daluga yang diperkuat oleh CNC lebih lambat baik yang  dalam tanah maupun yang ada dalam larutan EM4. Keterbaruan dari penelitian ini adalah pengujian biodegradibilitas dari bioplastik yang menggunakan sumber pati underutilized berbasis kearifan lokal yang diperkuat (reinforced) strukturnya dengan CNC asal limbah pengolahan pati daluga.
Water Quality of Ranowangko River That Crosses Tomohon City and Minahasa Regency, North Sulawesi Province Tumanduk, Yahya Y.R.; Naharia, Orbanus; Sumampouw, Herry M.; Ogi, Nova L.I.M.; Gedoan, Sukmarayu P.; Mokosuli, Yermia S.
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6119

Abstract

The Ranowangko River passes through Tomohon City and Minahasa Regency, ending in the Sulawesi Sea. Tombariri District, is the Bunaken National Park area. Ranowangko River Water Monitoring in 2022 by the Regional Environmental Service of North Sulawesi Province shows that Fecal Coli and Total Coli parameters in the Ranowangko River at Station 1 do not meet the requirements according to Government Regulation Number 22 of 2021 concerning Class 2 National Water Quality Standards (BMA). Research to assess water quality from biological, physical and chemical aspects as well as determine water quality status. Monitoring was carried out 4 times in 2 consecutive years at 3 observation stations. Quantitative data and qualitative data include water quality analysis through determination of 14 parameters compared according to BMA Class 2. The water quality data was analyzed using the Storet Method to determine water quality status in accordance with Minister of Environment Decree Number 115 of 2003. The results showed Fecal Coli and Total Coli (Biological Aspects), TSS (Physical Aspects), Total Phosphate (Chemical Aspects) exceeded the BMA. Score calculation results using the Storet method at Station 1: -68, Station 2: -72 and Station 3: -76. Based on the value system According to Minister of Environment Decree 115 of 2003, the score above shows that the Water Quality Status of the Ranowangko River is heavily polluted. Recommendations to the regional governments of Tomohon City and Minahasa Regency to carry out further monitoring of the water quality of the Ranowangko River.
Co-Authors Alfonds Andrew Maramis Anice I. Tuda Anita C. C. Tengker Anita Costanci Christine Tengker Arrijani - Britney Putri Hermanus Budiarto Carolin Manuahe Dewa Nyoman Oka Dina Munawaroh Dina Rombot Edieli Zebua Edyson Maneasa Emma M Moko Esibrena Br Kemit Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Febiola Gabriela Tular Febiola Najoan Ferencia Esananda Rattu Ferni Margo Tumbel Ferny Margo Tumbel Feydina Tewu Friscila Avianti Djaila Friska Mery Montolalu Futwembun, Fransiska Gedoan, Sukmarayu Piter Grace Jenny Soputan H. M. Sumampouw Hariyadi Hariyadi Helen J Lawalata Helen J Lawalata Helen Joan Lawalata Hendra Pratama Maliangkay Herry Maurits Sumampouw Ilham Saputra Butar-butar IRIANI SETYAWATI Jacklin Stella Manopo James S. Turambi Jantje Ngangi Jantje Pelealu Jeane Mantiri Jimmy Langi Jonathan Polandos julduz ruland paus Kamagi, Decky D. W. Kandowangko, Sandy Kaunang, Eva Sherly Nonke Keysia Stephanie Tesalonica Mulder Manampiring, Noni Mansyur, Djailani Masje Wurarah Masye Wurarah Max Tulung Meike Paat Meisa Tabita Rogahang Meity Nelltje Tanor Melita Irene Gracia Kaligis Migau, Natalina Mongdong, Priskila Indah Theresya Motulo, Serlin J Mundaeng, Camel Gantonio Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Ogi, Nova L.I.M. Orbanus Naharia Orbanus Naharia Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pua, Devis Christian Rahardiyan, Dino Revolson Mege Rolef Rumondor Rudi Alexander Repi Rymond Jusuf Rumampuk Sibala, Henike Simbolon, Kristina Br Sinyo Herry Sumampouw Suddin Simandjuntak Sukmarayu Gedoan Sukmarayu P. Gedoan Sumampouw, Harry Maurits Taariwuan, Marlin Bernadet Thesia Megi Kurniawati Rettob Timbuleng, Nobel Tjiakra, Rosalina A. Tripena Mamuaya Tuegeh, Reinny Silvana Tumanduk, Yahya Y.R. Utari Satiman Verawati I Y Roring Verawati Ida Yani Roring Verra E. J. Mawitjere Wisda Nurjannah Abdullah Worang, Rina L. Wuwungan, Axel Yessie K. Kelly