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Characterization of Pathogenic Bacteria Causing Bacterial Wilt Disease of Kepok Banana in Manokwari, West Papua Mogea, Rina; Puspasari, Aninda; Ruimassa, Reymas M R
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.4

Abstract

Kepok bananas, including their processed derivatives, serve as a vital alternative staple food alongside rice and contribute significantly to food security, particularly in the Manokwari region. Maintaining the health of banana plants is essential for preserving global food diversity. Ensuring their sustainable availability is crucial; however, this is increasingly threatened by the emergence of banana diseases. The rapid spread of banana diseases was first reported in 2018 in the SP IV Bowi Subur area of Masni District, and by 2019, similar cases were documented in the Anday area of South Manokwari District. This study aims to isolate and identify the pathogenic bacteria responsible for banana diseases in Kepok bananas from the Anday region of South Manokwari, using morphological and biochemical characterization. The macroscopic characteristics of the four isolates revealed a circular shape with entire margins, a convex surface, and a maroon coloration when cultured on CPG medium. Gram-staining analysis indicated that the bacterial cells were gram-negative and exhibited a short bacillus morphology. Isolation from samples collected from the banana rhizosphere, pseudostems, and fruits yielded four distinct isolates, designated as AR1, APS1, AR2, and ABF2. Based on nitrate reduction and gelatin hydrolysis tests, isolates AR1, AR2, and ABF2 were identified as belonging to the Blood Disease Bacterium (BDB), specifically Ralstonia syzygii phylotype IV (Indonesia), which has undergone taxonomic reclassification as Ralstonia syzygii subsp. celebesensis. This disease can result in crop losses exceeding 50% when it spreads within a consortium. In contrast, the APS1 isolate could not be conclusively identified as either R. syzygii subsp. celebesensis or R. solanacearum.
Hubungan komunitas zooplankton dengan faktor oseanografi di ekosistem terumbu karang Manokwari S. Tururaja, Tresia; Bawole, Roni; Mogea, Rina; Murtihapsari
Cassowary Vol 8 No 3 (2025): Juli
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v8.i3.484

Abstract

Zooplankton play an important dual role in marine ecosystems as food webs connectors and sensitive indicators of water quality. This study examines the composition, abundance, and relationship of zooplankton communities with oceanographic parameters in the coral reef ecosystem of Manokwari Waters. Samples were collected from three observation stations: Kaki Island, Lemon Island, and Marampa Port, using a 25 μm plankton net. Morphological identification resulted in six classes of zooplankton, namely Hexanauplia, Gastropoda, Bivalve, Malacostraca, Branchiopoda, and Ostracoda, for a total of 23 species. The class Hexanauplia dominates in all locations, with the most abundant species being Undinula vulgaris (10 individuals), Calanus sinicus (7), and Eurytemora pacifica (6). Cluster analysis based on the Bray-Curtis similarity index with the Average Linkage method shows the similarity of zooplankton communities between Marampa Port and Lemon Island. Kaki Island forms a separate cluster, indicating differences in species composition spatially. The highest abundance was recorded in the Hexanauplia class with an abundance index of >2,500 cells/L in some species. Canonical Correspondence Analysis (CCA) revealed a positive correlation between DO and Bivalvia (+0.99), temperature with Gastropoda (+0.92), and salinity with Hexanauplia (+0.28). Environmental factors such as temperature, DO, pH, and depth have been shown to influence the spatial distribution and diversity of communities. These findings reinforce the dual role of zooplankton as a fundamental food source for benthic organisms such as the soft coral Sarcophyton sp. and as an effective bioindicator for monitoring the health of tropical coastal ecosystems. Keywords: zooplankton, Undinula vulgaris, Hexanauplia, oceanographic parameters, Manokwari
Keragaman fungi rhizosfer tanaman pisang kepok yang terinfeksi penyakit darah (Rastolnia syzygii subsp. celebesensis) di Bremi, Distrik Manokwari Utara, Papua Barat Puspasari, Aninda; Mogea, Rina; M. R. Ruimassa, Reimas
Cassowary Vol 8 No 4 (2025): Oktober
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v8.i4.462

Abstract

ABSTRACT: Blood disease is a significant problem in Kepok banana cultivation, resulting in substantial economic losses. Blood disease is caused by the pathogenic bacteria Rastolnia syzygii subsp. celebecensis, a soil-borne disease. Soil microbes play a crucial role in the biocontrol process because they can produce certain enzymes or metabolites that can suppress disease-causing pathogens in plants. One approach is the use of fungi as antagonistic biological agents that can protect plants from disease attack. The presence of fungal biological agents in the rhizosphere of Kepok banana plants infected with blood disease requires isolation and identification of soil fungi to obtain data on the diversity of indigenous rhizosphere fungi in Kepok banana plants infected with blood disease in Bremi, North Manokwari District. The results of the isolation and identification of rhizosphere fungi in Kepok banana plants infected with blood disease yielded six (6) fungal isolates with high disease virulence and eleven (11) fungal isolates with moderate disease virulence. The fungal isolates obtained from the rhizosphere of high disease virulence were identified as Trichoderma sp1., Rhizoctonia sp., Aspergillus sp1., Aspergillus sp2., Trichoderma sp2., and Trichoderma sp3. Fungal isolates obtained from the rhizosphere of moderate disease virulence were identified as Fusarium sp1., Fusarium sp2., Penicillium sp1., Aspergillus sp1., Aspergillus sp2., Trichoderma sp1., Trichoderma sp2., Trichoderma sp3., Fusarium sp3., Penicillium sp2., and Penicillium sp3.
Konservasi terumbu karang dengan bioreeftek di Perairan Kampung Bakaro Manokwari Papua Barat: Coral reefs conservation with bioreeftech in the Beach of Bakaro Village Manokwari West Papua Mogea, Rina; Noya, Alce I.; Tururaja, Tresia S.; Lamadi, Rahmat; Tandililing, Jeanifer G.; Puspasari, Aninda; Maahury, Sevian A.; Bakri, Iskandar
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 5 No. 3 (2024): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v5i3.479

Abstract

ABSTRACT  Bakaro Beach is one of the beach tourist destinations in Manokwari and is known as a fish calling place. Unfortunately this place was damaged due to fishing using bombs or tubas. The aim of this service activity is to provide an understanding of the importance of coral reef ecosystems and how to restore coral reefs using bioreeftek, ie technology that uses coconut shells as a medium for attaching coral planula larvae to form new individuals (coral). This is done to create new habitat or restore damaged coral. This technology is easy because it uses simple tools, using coconut shells as the main material and the process is not difficult, besides all the materials used are environmentally friendly. Coconut shells function as a place or container for coral planula larvae to settle after the larvae attach to the coconut shell substrate and are then transferred to damaged coral reefs. With the development of bioreeftek, it has become a new focus in preserving coral reef ecosystems.  Keywords: Coral Reef Restoration; Bioreeftek; Coconut Shells; Bakaro Beach; Environmental Conservation   ABSTRAK Pantai Bakaro tempat ini sebagai salah satu destinasi wisata pantai yang ada di Manokwari dan dikenal dengan tempat pemanggilan ikan.  Sayangnya tempat ini mengalami kerusakan akibat pengambilan ikan dengan menggunakan bom atau tuba. Tujuan kegiatan pengabdian ini yaitu memberi pemahaman mengenai pentingnya ekosistem terumbu karang dan cara melakukan pemulihan terumbu karang dengan bioreeftek yaitu teknologi yang memakai tempurung kelapa sebagai media menempelnya larva planula karang sampai membentuk individu baru (karang). Hal ini dilakukan untuk menciptakan habitat baru atau memulihkan kembali karang yang rusak.  Teknologi ini mudah sebab menggunakan alat sederhana yaitu tempurung kelapa sebagai bahan utama dan proses pengerjaannya tidak sulit, disamping itu juga semua bahan yang digunakan ramah lingkungan. Tempurung kelapa berfungsi untuk tempat atau wadah dari larva planula karang menetap setelah larvanya menempel pada subtrat tempurung kelapa maka dipindahkan ke terumbuh karang yang rusak. Dengan menggembangkan bioreeftek ini menjadi tumpuan baru dalam melestarikan ekosistem terumbu karang. Kata kunci: Pemulihan Terumbu Karang; Bioreeftek; Tempurung Kelapa; Pantai Bakaro; Pelestarian Lingkungan 
Co-Authors Adinda Rindiani Putri Ai Siti Patimah Alce I. Noya Amestina Matualage Andoyo Supriyantono Aradea Bujana Kusuma, Aradea Bujana Astriet Y. Manangkoda Bakri, Iskandar Barahima Abbas Barahima Abbas Bimo Budi Santoso D. Wasgito Purnomo Daniela Ijie Darma Santi Daud Bano Deasy Erawati Dian Kristin Dwiranti, Febriza Eko Agus Martanto Frengky Krey George Yarangga Hendri Hendri Hermawaty Abubakar Ishak Musaad Kolibongso, Duaitd Lamadi, Rahmat M. R. Ruimassa, Reimas Maahury, Sevian A. Manan, Jemmy Maria Elisabeth Krey Maria Massora Markus Heryanto Langsa Martha Kayadoe Mawikere, Nouke L. Moeljono, Soetjipto Mogea, Rinondom AR Murtihapsari Murtihapsari . Musaad, Ishak Nouke L. Mawikere Noya, Alce I. Nuari Rante Tondok Nurul Abidin Nurul Endang Lestari Nurul Muzizah Nuryanti Rumalolas Panjaitan, Rawati Panjaitan, Rawati Paskalina Theresia Lefaan Paulus Boli Paulus Boli Paulus Boli Purba, Gandi YS Purba, Gandy Y. S. Puspasari, Aninda Ratnawati, Sita Rein M. R. Ruimassa Reymas M.R. Ruimassa Ridwan Sala Robi Binur Roni Bawole Ruimassa, Reymas M R Ruimassa, Reymas M.R. S. Tururaja, Tresia Simon P.O Leatemia Simon Sutarno Sinaga, Nurhaida I. Sinuraya, Sabarita Sonya F. Nauw Sri Hartini Suawa, Elfira K. Suparno, Antonius Syafrudin Raharjo Syufi, Yafed Tandililing, Jeanifer G. Tirza Bandi Tresia Tururaja Trisiwi Wahyu Widayati Tururaja, Tresia S. Tururaja, Tresia Sonia Vanny H. Siwi Vera Sabariah Vera Sabariah, Vera WAHYUDI Waode Intan Cendrasari La Halim Putri Wibawati, Zarima Yafed Syufi Yamco, Fallen Yeni Salosa Yenni Yendry Salosa Yolanda Holle Yuanike Kabera, Yuanike Zarima Wibawati Zita L. Sarungallo