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WAODE MUNAENI
Universitas Khairun, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Microbiology Indonesia

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Effect of Micro-encapsulated Synbiotic at Different Frequencies for Luminous Vibriosis Control in White Shrimp (Litopenaeus vannamei) WAODE MUNAENI; MUNTI YUHANA; WIDANARNI WIDANARNI
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.24 KB) | DOI: 10.5454/mi.8.2.5

Abstract

The aim of this study was to evaluate the effect of micro-encapsulated synbiotic application at different frequencies for luminous disease control in white shrimp (Litopenaeus vannamei). The luminous disease is caused by Vibrio harveyi. In this experiment, a synbiotic which was a combination of the probiotic Bacillus sp. NP5 RfR and the oligosaccharides from sweet potato (Ipomea batatas L.) jago variety was apllied. The synbiotic was encapsulated by spray drying method. The in vivo experiment was conducted by supplementing the shrimp’s diet with the micro-encapsulated synbiotic for 40 days. Treatments included the administration micro-encapsulated synbiotic in different frequencies i.e. once a week (A), twice a week (B), daily (C), and without micro-encapsulated synbiotic (control treatment). The control treatment consisted of positive (K+) and negative (K-) controls. After 30 days period, all of the shrimp were challenged by intramuscular injection of pathogenic V. harveyi RfR at a concentration of 106 CFU ml-1 except the negative control. The treatment C resulted in significantly higher survival rate (SR), specific growth rate (SGR), and immune responses than those of the controls, whereas the feed conversion ratio (FCR) was lower than the controls. In addition, the population of Bacillus sp. NP5 RfR and total bacterial count (TBC) in the intestines increased, whereas the population of V. harveyi RfR and the total vibrio count (TVC) were lower compared to the controls.
In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi WAODE MUNAENI; ARMAN PARIAKAN; LAODE BAYTUL ABIDIN; MUNTI YUHANA
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.507 KB) | DOI: 10.5454/mi.11.3.1

Abstract

The objectives of this study were to analyze phytochemical content of bawang hutan bulbs extract (Eleutherine palmifolia) and to test the inhibitory potential of bawang hutan bulbs extract on the growth of Vibrio harveyi bacteria at different doses. This study was conducted in March-May 2017 in Testing Laboratory of Fisheries and Marine Science Faculty of Halu Oleo University and Laboratory of Fish Health of Aquaculture Department of Fisheries and Marine Science Faculty and Laboratory of Biopharmaca of Bogor Agricultural University. Test parameter included: (1) Phytochemical test through the method of color visualization, (2) Inhibitory potential test using two methods namely agar diffusion and co-culture. Treatment of dose consisted of positive control/K+ (Chloramphenicol 30 mg/ml), negative control/K- (Sterile Aquadest) and treatment of extract included A (20 mg/ml), B (40 mg/ml), C (60 mg/ml), D (80 mg/ml). Qualitatively, result of phytochemical test showed that bawang hutan bulbs extract contained flavonoid, tannin, saponin, quinone, steroid and triterpenoid compounds. Result of inhibitory potential test indicated that treatment D obtained the highest inhibitory potential, while the minimum inhibitory potential was found in treatment A. The best co-culture test result was also found in treatment D, in which 24 hours after co-culture was performed, no V. harveyi colonies (total bacteria of 0 CFU/mL) were found. Bawang hutan bulbs extract in this study was able to inhibit the growth of V. harveyi.
Co-Authors . Tamrin Abdullah, Nursanti Adriani, Rovina Agustinus Herlis Maholle Ahmad, Khamsiah Aras Syazili Aris Tri Wahyudi Aris, M. Aris Aris, Muhammad Darsan, Ismi Musdalifah Daud, Asmar Hi Disnawati Disnawati Disnawati Disnawati Disnawati, Disnawati Eko Harianto, Eko Febrina Rolin Haji, Sulfi Abdul Ismi Musdalifah Darsan Juharni, Juharni La Ode Baytul Abidin Labenua, Rusmawati M IRFAN, M M. Aris Malan, Sudirto Maryani, Maryani Mia Setiawati Muchdar, Fatma Mufti Abdul Murhum Muh. Aris Muhammad Aris Muhammad Aris MUNTI YUHANA Pangentasari, Dwinda Pariakan, Arman Rahmatullah, Miftahul Riyadi Subur, Riyadi Rovina Ruslami, Rovina Rusmawati Labenua Rusmawati Labenua Safratilofa Safratilofa, Safratilofa Samadan, Gamal M Sudirto Malan Sulfi Abdul Haji Suryani Suryani Syafari, Muhamad Rismon Tamrin Tamrin WIDANARNI WIDANARNI Yuliana Yuliana