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Anas Fadli Wijaya
Universitas Dr. Soebandi
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Hubungan Pengetahuan dan Kepatuhan Cuci Tangan terhadap Kejadian Demam Tifoid di Kabupaten Tulungagung Nurjanah, Mutia Hariani; Wijaya, Anas Fadli; Fuzianingsih, Eka Novia; Rahmawati, Ayu Sintya Citra; Martha, Rahma Diyan
JURNAL BIOSHELL Vol 14 No 2 (2025): Oktober
Publisher : Universitas Islam Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/bio.v14i2.4572

Abstract

Demam tifoid adalah penyakit infeksi yang ditimbulkan oleh bakteri Salmonella typhi dan menyebar melalui makanan atau minuman yang terkontaminasi tinja atau urine yang mengandung bakteri tersebut. Penyakit ini termasuk ke dalam kategori penyakit menular (transmissible disease). Gejala utama yang biasa muncul antara lain mual, nyeri pada perut, gangguan buang air besar seperti diare atau sembelit, serta demam tinggi. Penelitian ini bertujuan untuk mengetahui hubungan antara cuci tangan dengan kejadian demam tifoid. Penelitian dilakukan dengan desain pendekatan cross-sectional dan melibatkan 30 responden. Berdasarkan hasil analisis menggunakan uji chi-square, diperoleh nilai p = 0,004, yang menunjukkan adanya hubungan yang signifikan antara kebersihan diri dan kejadian demam tifoid, serta faktor lingkungan. Kesimpulan: Terdapat hubungan yang signifikan antara kebersihan diri dengan kejadian demam tifoid. Sebagian besar partisipan dalam penelitian ini adalah perempuan, dengan rentang usia terbanyak berada antara 5 hingga 13 tahun.
Pemeriksaan Kesehatan Pada Lansia di Dusun Kandangan Rambipuji Jember: Pemeriksaan Kesehatan Pada Lansia di Dusun Kandangan Rambipuji Jember Wijaya, Anas Fadli; Mutiurrohman, Achmad; Ikhsan, Ahmad Mufti; Putri, Diva Amalia; Hanifiyah, Fitroh; Rossa, Handita Emilia; Kamaliyah, Indy; M Syaifuddin; Wafa, Muhammad Ali; Soleha, Nurmaidatus; Maimunah, Siti; Holifah, Siti Nur; Sofia
Jurnal Pengabdian Masyarakat Kesehatan Terkini Vol 3 No 2 (2024): Jurnal Pengabdian Masyarakat Kesehatan Terkini
Publisher : Ruang Ide Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58516/5w046437

Abstract

Elderly individuals are a vulnerable age group prone to various health issues due to the aging process, such as hypertension, diabetes mellitus, and uric acid disorders. Low health awareness and economic constraints lead to infrequent health check-ups among the elderly, resulting in missed opportunities for early disease detection. This community service activity aims to conduct health screenings for the elderly, focusing on blood pressure measurement, blood glucose testing, and uric acid levels in Dusun Kandangan, Pecoro Village, Rambipuji District, Jember Regency, on August 21, 2024. The methods employed include blood pressure measurement using a sphygmomanometer, blood glucose testing, and uric acid testing using test strips. Of the 27 elderly individuals examined, 15 were found to have hypertension, 8 showed symptoms of diabetes mellitus, and 10 had elevated uric acid levels. This activity successfully identified health issues among the elderly.
Gambaran Morfologi Sel Neutrofil Pada Pewarnaan Giemsa dengan Variasi Waktu Pada Larutan Pengencer Akuades Muflihah, Ahdiah Imroatul; Destiawan, Rian Anggia; Wijaya, Anas Fadli; Widia, Leny Yulia; sufi, Queen Nurul; Azizah, Laila Camelia Nur; Makki, Alisa Alfiya
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 1 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i1.1470

Abstract

Giemsa staining is a microscopic staining technique that aims to identify the morphology of leukocyte cell types. One of the methods that uses giemsa staining is the method of examining peripheral blood smear preparations which aims to evaluate the calculation of leukocyte type. The dyeing quality of giemsa is greatly influenced by the type of diluent material, namely buffer and aquadest. This study aims to determine the morphological picture of neutrophil cells in giema staining with time variations in aquadest diluent solution. The method of this study was to take blood from adults aged 20-25 years and had no history of illness as many as 24 samples. It was divided into 2 dilution groups, namely pH buffer 6.8 and aquadest, where each dilution was divided into 4 groups, namely group 1 soaking the peripheral blood smear preparation for 10 minutes, group 2 soaking the peripheral blood smear preparation for 20 minutes, group 3 soaking the peripheral blood smear preparation for 30 minutes, group 4 soaking the peripheral blood smear preparation for 40 minutes. Then a qualitative analysis was carried out, namely by comparing the background of the preparation and the morphology of neutrophil cells microscopically in each group. The results of this study are that microscopically groups 1 and 2 have a clear preparation background, while in groups 3 and 4 the preparation background is dirty and there are neutrophil granules and morphology in groups 1 and 2 show dark blue neutropnhil cells, groups 3 and 4 show purple and bluish-purple neutrophil cells. The conclusion of this study is based on the background of the preparation, and the morphology of neutrophil cells, the group that has the best results is groups 1 and 2. Key words : Aquadest, eosinophil cells, eutrophil cells, Giemsa staining
The Effect of Differences Time On The Macroscopic Picture Of Giemsa Staining Using Aquades Diluent Muflihah, Ahdiah Imroatul; Rian Anggia Destiawan; Anas Fadli Wijaya; Lenny Yulia Widia Sari; Queen Nurul Sufi; Alisa alfiya makki; Laila Camelia
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.678 KB)

Abstract

Peripheral Blood Smear Preparation (SADT) is an examination used to see the structure and number of red and white blood cells, besides giemsa staining can be used for malaria examination. The quality of giemsa staining is influenced by one of which is the soaking time of the giemsa solution. The purpose of this study was to determine the quality of slides or blood smears based on the incubation duration of giemsa staining. This research method qualitatively performed giemsa staining on blood smears with different soaking times (10 minutes, 20 minutes, 30 meinit and 40 minutes) then compared with controls. Results showed that at minutes 10 and 20 the giemsa staining showed a smooth surface and no granules, at 30 and 40 minutes the giemsa staining showed a rough surface and many granules. The conclusion of this study was that 10 and 20 min giemsa soaking showed optimal staining of blood smear preparations.    
Hubungan Kejadian Typoid Fever dengan Kebersihan Diri Pada Usia 5-45 Tahun di Tulungagung Nurjanah, Mutia Hariani; Wijaya, Anas Fadli; Safitri, Yunita Diyah; Santoso, Arif; Destiawan, Rian Anggia
Innovative: Journal Of Social Science Research Vol. 4 No. 6 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i6.14007

Abstract

\ Typoid fever adalah infeksi bakteri yang disebabkan oleh bakteri S. Typhi dengan mengkonsumsi makanan atau air yang terkontaminasi oleh feaces atau saluran kemih yang terdapat bakteri. Gangguan ini menular langsung yang disebut dengan transmissible disease. Gejala utama infeksi ialah mual, sakit perut, dan buang air besar diare maupun sembelit dengan disertai demam tinggi. Kekambuhan mungkin lebih ringan dibandingkan infeksi awal, namun dapat menyebabkan gejala yang lebih parah dibandingkan infeksi awal.  Kekambuhan ini bisa ringan atau berat dan bisa terjadi hingga dua atau tiga kali. Selain itu, Komplikasi dapat terjadi da berdampak jika demam tifoid jika tidak ditangani dengan tepat sasaran. Tujuan untuk mengetahui hubungan kejadian typoid fever dan kebersihan diri. Metode menggunakan metode pendekatan cross secssional dengan jumlah responden sebanyak 30 partisipan. Hasil menggunakan uji chi square menunjukkan bahwa kejadian  typoid fever dan kebersihan diri p = 0.001 menunjukkan adanya hubungan demam tifoid dengan kebersihan diri dan lingkungan. Kesimpulan : Terdapat hubungan antara typhoid fever dan kebersihan diri. Partisipan paling banyak perempuan rentang usia paling tinggi 5-15 tahun.
Pemeriksaan Kesehatan Dan Edukasi Kesehatan Masyarakat Desa Kamal Dalam Upaya Mengontrol Kadar Gula Darah Aggustin, Ayu Tri; Wijaya, Anas Fadli; Muflihah, Ahdiah Imroatul; Hidayati, Sholihatil
PRAXIS: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 3 (2024): PRAXIS April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47776/praxis.v2i3.943

Abstract

This community service activity is a form of effort to support the government program "Healthy Communities that are Independent and Just. The target subjects for partners in this community service activity are the people in Kamal Village, Arjasa District, and Jember Regency. Preliminary studies have been carried out and show that the incidence rate in the village is very high. Thus, health checks and health promotion in an effort to control blood sugar levels through the use of natural germplasm are very necessary. The health checks that will be carried out include random blood sugar checks (GDS). The education provided can include understanding the causes, signs and symptoms, risk factors, and types of healthy nutrition to prevent and control complications of diabetes mellitus. Early screening can be done by carrying out laboratory examinations in the community. After the examination, it was discovered that some people experienced hyperglycemia and some experienced hypoglycemia. Through this activity, the public can understand the material presented and can find out their health status so that early prevention can be carried out.
Evaluation of (Tinomiscium petiolare Hook.F. &Thomson) Extract Ointment on Inflammation and Angiogenesis During Incision Wound Healing Hidayati, Larantika; Romaidha, Iqlila; Wijaya, Anas Fadli; Sucipto, Ade; Laksono, Eko Budi
Indonesian Journal of Global Health Research Vol 7 No 2 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i2.5339

Abstract

Wound incidence remains high worldwide and includes surgical injuries, burns, ulcers and trauma. National reports also show that abrasions and contusions account for more than half of wound complaints. These data indicate a continuing need for effective therapeutic approaches. Wound healing depends on a regulated inflammatory phase and sufficient angiogenesis, and disturbances in these processes can delay tissue repair. Natural compounds with anti-inflammatory and pro-angiogenic activity offer potential support. Tinomiscium petiolare contains flavonoids and alkaloids with documented roles in modulating inflammation and promoting vascular growth. Evidence regarding its effects on incision wounds is limited and requires systematic evaluation. This study aimed to evaluate the wound-healing effects of Tinomiscium petiolare extract ointment by examining inflammatory and angiogenic responses in incision wounds with and without Staphylococcus aureus infection. Twenty-four male Rattus norvegicus were randomized into four groups consisting of non-infected controls, infected controls, extract-treated non-infected rats and extract-treated infected rats. Standardized incision wounds were created, infection was induced in designated groups and the extract ointment was applied to treatment groups. Tissue samples were collected on days 3 and 7 to measure TNF-α expression, macrophage infiltration and blood vessel formation. Statistical analysis included normality testing, variance homogeneity testing and comparative analyses with post-hoc procedures when required. TNF α levels showed no group differences on day 7. Temporal analysis demonstrated significant reductions from day 3 to day 7 in T3 and T4 (p < 0.05). Macrophage infiltration differed among groups (p = 0.044), and post-hoc analysis indicated significantly lower macrophage counts in T4 compared with T1 after Bonferroni correction. Blood vessel density also varied among groups (p = 0.019), and temporal evaluation showed a progressive increase in angiogenesis in T4 from day 3 to day 7, although pairwise comparisons did not reach statistical significance. The extract modulated inflammatory activity and supported angiogenic progression, with the strongest effects in infected wounds treated with the ointment.
The Effect of Papaya Leaf Extract (Carica papaya L.) on Erythrocyte Values in Anemia-Model Rats Dwi Susanto, Fajar; Yulia Widia Sari, Leny; Imroatul Muflihah, Ahdiah; Wijaya, Anas Fadli; Agustin, Ayu Tri
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 2 (2025): Desember
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i2.34

Abstract

Background: Anemia remains a significant public health challenge with a global prevalence of 40-88%. Papaya leaf extract (Carica papaya L.) contains bioactive compounds such as flavonoids, vitamin C, and vitamin E that potentially enhance erythrocyte production. Purpose: This study aimed to determine the effect of papaya leaf extract on erythrocyte values in anemia-model rats (Rattus novergicus). Methods: An experimental laboratory study was conducted from April to June 2025 using 25 rats divided into 5 groups: positive control (sodium nitrite), negative control (aquadest), and three treatment groups receiving papaya leaf extract at doses of 100, 200, and 400 mg/kg BW. Anemia was induced using sodium nitrite at 50 mg/kg BW via intraperitoneal injection. Erythrocyte measurements were performed using a hematology analyzer. Data were analyzed using Shapiro-Wilk normality test, Levene's homogeneity test, one-way ANOVA followed by Tukey HSD (α=0.05). Results: Treatment group P3 (dose 400 mg/kg BW) demonstrated the highest erythrocyte value of 12.066±0.84 (10⁶/µL), followed by P1 (11.576±0.71), P2 (11.496±1.19), negative control (10.728±1.01), and positive control (6.220±1.96). ANOVA test revealed significant differences between groups (p<0.001). Conclusions: Papaya leaf extract increases erythrocyte values in anemia-model rats, with 400 mg/kg BW being the most effective dose.
Analisis Pemantapan Mutu Internal Pada Pemeriksaan Trombosit Dan Leukosit Menggunakan Metode Sigma Metric Di Puskesmas X Muflihah, Ahdiah Imroatul; Muhammad Wildanun Ulum; Anas Fadli Wijaya; Hartalina Mufidah
Jurnal Analis Kesehatan Vol. 14 No. 2 (2025): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v14i2.5458

Abstract

Pemantapan mutu internal (PMI) di laboratorium krusial dilakukan untuk pengawasan dan pencegahan kesalahan hasil pemeriksaan, salah satu PMI dilakukan pada pemeriksaan trombosit dan leukosit menggunakan hematology analyzer untuk pencegahan kesalahan baca. Penelitian ini bertujuan untuk menganalisis hasil kontrol kualitas berapa akurasi, presisi, grafik Levey Jenning dengan aturan Westgard dan Sigma Metrik. Metode pada penelitian ini menggunakan metode deksriptif kuantitatif dengan menggunakan data sekunder pemeriksaan kontrol trombosit dan leukosit pada bulan Juli - Oktober 2024. Data kontrol pemeriksaan trombosit dan leukosit dianalisis berdasarkan aturan Westgard dan Sigma Metrik. Hasil pemantapan mutu internal pada penelitian ini menunjukkan bahwa keduanya memiliki tingkat akurasi yang baik karena berada dalam batas bias ±10%. Nilai presisi memenuhi kriteria karena tidak keluar dari nilai CV >5%. Evaluasi grafik Levey–Jennings berdasarkan aturan Westgard tidak menunjukkan pelanggaran aturan. Nilai Sigma Metrik untuk trombosit berada pada kategori sangat baik (>6), sedangkan nilai sigma metrik untuk leukosit berada pada kisaran >2 yang masih dapat dinilai layak untuk digunakan dalam pemeriksaan sampel.
Antibacterial Activity of Papaya (Carica papaya L.) Seed Ethanol Extract against Staphylococcus aureus Isolated from Diabetic Ulcers Putri Aprilia, Diah; Mufidah, Hartalina; Wijaya, Anas Fadli
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.30

Abstract

Background: Staphylococcus aureus is a bacterium that causes infections and is pathogenic to humans. Papaya (Carica papaya L.) seed extract contains various bioactive compounds, including tocopherols, terpenoids, flavonoids, alkaloids such as carpaine, and enzymes such as papain and lysozyme, which are known for their potential antimicrobial effects. Purpose: This study aimed to determine the antibacterial activity of papaya seed ethanol extract against S. aureus isolated from the foot ulcers of diabetic patients. Methods: An experimental quantitative study was conducted to evaluate the antibacterial activity of papaya seed ethanol extract at concentrations of 40%, 60%, and 80%. The extracts were prepared using the Ultrasonic-Assisted Extraction (UAE) method. Antibacterial activity was assessed by measuring the inhibition zones formed against S. aureus. Data were analyzed using a one-way ANOVA followed by a post hoc test to determine statistical significance. Results: The papaya seed extract exhibited concentration-dependent antibacterial activity against S. aureus. The mean inhibition zones were 0.00 mm at 40%, 15.07 mm at 60%, and 18.83 mm at 80%, whereas the positive control produced an inhibition zone of 46.36 mm. Conclusions: Papaya seed ethanol extract demonstrated antibacterial activity against S. aureus isolated from diabetic foot ulcers, with the strongest inhibition observed at the 80% concentration.
Co-Authors Ade Sucipto Aggustin, Ayu Tri Agustin, Ayu Tri AHDIAH IMROATUL MUFLIHAH Ahdiah Imroatul Muflihah Alisa Alfiya Makki Arif Santoso Azizah, Laila Camelia Nur Delvia Asista Indarusanti Destiawan, Rian Anggia Dwi Susanto, Fajar Elnaja, Dinar Anisa Fuzianingsih, Eka Novia Hanifiyah, Fitroh Hartalina Mufidah Hayati, Farhatul Hidayati, Larantika Hidayati, Sholihatil Holifah, Siti Nur Humairoh, Riska Tsamarah Ikhsan, Ahmad Mufti Imroatul Muflihah, Ahdiah Kamaliyah, Indy Kumalasari, Nurul Chamidah Laila Camelia Laila Camelia Nur Azizah Laksono, Eko Budi Lenny Yulia Widia Sari Leny Yulia Widia M Syaifuddin M. Elyas Arif Budiman Makki, Alisa Alfiya Maruni Wiwin Diarti Meri, Meri Mufidah, Hartalina Muflihah, Ahdiah Imroatul Muhammad Wildanun Ulum Mutia Hariani Nurjanah Mutiurrohman, Achmad Ni Ketut Yuliana Sari Nosa Ika Cahyariza Nosa Ika Cahyariza Nurjanah, Mutia Hariani Pratikah Verdianti Purwanti, Ananda Kamila Nurcahya Putri Aprilia, Diah Putri, Diva Amalia Queen Nurul Sufi Rahma Diyan Martha Rahmawati, Ayu Sintya Citra Rahmawati, Septyana Eka RIAN ANGGIA DESTIAWAN Rian Anggia Destiawan Romaidha, Iqlila Rossa, Handita Emilia Safitri, Yunita Diyah Sholihatil Hidayati Sigit Purwanto Siswidiani, Maya Diva Siti Maimunah Sofia Soleha, Nurmaidatus sufi, Queen Nurul Sulastri Sulastri Tilarso, Dara Pranidya Urip Urip Wafa, Muhammad Ali Widayanti, Sindi Dwi Widia, Leny Yulia Yulia Widia Sari, Leny Yunan Jiwintarum