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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Lingkungan Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Abdimas Indonesian Journal Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan South East Asian Marine Sciences Journal Jurnal Perikanan dan Kelautan Jurnal Natur Indonesia
Nursyirwani, Nursyirwani
Universitas Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Veterinary Other

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ISOLATION, IDENTIFICATION AND SENSITIVITY OF AMILOLITIC BACTERIA FROM MANGROVE ECOSYSTEM SEDIMENT IN PURNAMA MARINE STATION DUMAI ON THE PATHOGENIC BACTERIA Silitonga, Lamtiur Rotua; Nursyirwani, Nursyirwani; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

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Abstract

Litter from the weathering of dead mangrove stems and leaves contains a lot of starch which has potential to be degraded by amylolytic bacteria into simple compounds with the help of the amylase enzyme. Amylolytic bacteria are bacteria that hydrolyze starch into simpler compounds namely glucose with the help of the amylase enzyme. This study aims to 1) isolate, identify and test sensitivity of amylolytic bacterial isolates found at the Purnama Dumai Marine Station, 2) the ability of amylolytic bacterial isolates to inhibit the growth of pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Vibrio alginolyticus) and 3) to determine the of amylolytic bacterial species by 16S rRNA sequence analysis. The results showed 10 bacterial isolates (TR 2, TR 6, TR 7, TR 9, TR 11, TR 13, TR 15, TR 16, TR 18 and TR 20) were able to inhibit the growth of pathogenic bacteria (E.coli, P.aeruginosa and V.alginolyticus). The sensitivity test of isolate TR 20 against E.coli was categorized into weak with inhibition zone diameter of 4.65 mm. Sensitivity of isolate TR 6 against P.aeruginosa was categorized into medium with inhibition zone diameter of 5.22 mm. Then sensitivity of isolate TR 11 against V.algynolyticus was categorized into medium with inhibition zone diameter of 5.55 mm. DNA analysis using 16S rRNA method and BLAST analysis showed similarity of each isolate. Isolate TR 6 was similar to Bacillus paramycoides strain MCCC 1A04098, isolate TR 11 was in a group of Enterobacter cloacae strain ATCC 13047 and TR 20 was in a group of Vibrio harveyi strains of NBRC 15634
ISOLATION OF LACTIC ACID BACTERIA FROM VANAME SHRIMP (Litopenaeus vannamei) AND ITS ANTAGONISM TO PATHOGEN BACTERIA Sufaidah, Siti Nur; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vaname shrimp is one of Indonesia's leading export species and is widely cultivated. The main problem in vaname shrimp pond cultivation is the attack of shrimp diseases caused by bacteria or viruses. This study aims to isolate and identify lactic acid bacteria (BAL) from vaname shrimp (Litopenaeus vannamei) and test their antagonism against pathogenic bacteria. The research was conducted from February to April 2023. The method used in this study is a survey method. Sampling was conducted at the vaname shrimp pond of the Fisheries Service Task Unit (UPT), Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The method used in this research was a survey method. BAL was isolated using MRS Agar + CaCO3 media. The Kirby-Bauer method was used to test bacterial antagonism against pathogenic bacteria. Based on the identification of seven isolates, it was found that only four isolates were positively identified as LAB bacteria, namely 47D, 54T1, 73DB1, and 73BD2. The results of the antagonism test against pathogenic bacteria showed that all BAL isolates could not suppress the growth of E. coli bacteria. However, these isolates suppressed the growth of A. hydrophila bacteria, with the widest inhibition zone diameter on isolate 54T1 (4.2 mm). On Vibrio sp. bacteria, the widest inhibition zone of BAL was on isolate 73DB1 (6 mm). These results indicate that the antibacterial activity produced by BAL is still relatively weak.
ISOLATION OF Escherichia coli FROM VANAME SHRIMP (Litopenaeus vannamei) POND AND THE SENSITIVITY TOWARD ANTIBIOTICS Putri, Resty Fauziah; Nursyirwani, Nursyirwani; Batubara, Ummi Mardhiah
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp (Litopenaeus vannamei) is a fishery commodity widely cultivated in various parts of Indonesia. The main problem often found in vannamei shrimp production is poor water quality during the rearing period. This study aims to isolate and identify E. coli contained in water, sediment, and meat of vannamei shrimp cultivated in shrimp ponds and examine its sensitivity to antibiotics. The research was conducted from March to May 2023 at the Technical Implementation Unit (UPT) Vannamei Shrimp Ponds of the Maritime Affairs and Fisheries Service, Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The survey method was used in this study. The Most Probable Number (MPN) method was employed for E. coli isolation to calculate bacterial density, and the Kirby-Bauer method was used to test bacterial sensitivity to antibiotics. Based on the identification of the six isolates carried out, it was found that only two isolates were positively identified as E. coli bacteria, namely SD C 3.1 and IP B 1.1, which were isolated from sediment and the Wastewater Treatment Plant (WWTP) pond. The diameter of the inhibition zone for sediment isolate RF01 against the antibiotic chloramphenicol was 18.75 mm, indicating intermediate sensitivity. On the other hand, WWTP isolates RF02 exhibited an inhibition zone of 17 mm for chloramphenicol, indicating medium sensitivity as well. However, it showed resistance to penicillin with a diameter of 6.5 mm and resistance to ampicillin with a diameter of 2 mm.
ANALYSIS OF ORGANIC MATTER CONTENT IN WATER AND SEDIMENT IN THE EAST COAST WATERS OF BENGKALIS ISLAND, RIAU PROVINCE Lestari, Anggi; Amin, Bintal; Nursyirwani, Nursyirwani
Asian Journal of Aquatic Sciences Vol. 6 No. 2 (2023): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Organic matter is one indicator of environmental fertility both on land and at sea. The content of organic matter in a certain amount can be useful for aquatic biota, but if the amount of organic matter exceeds the assimilation capacity of the waters, it can cause a disturbance. This study aims to determine the organic matter content of water and sediment at high and low tide conditions in the waters of the east coast of Bengkalis Island and to determine the relationship. The method used is a survey method that is carried out in the field directly to collect data and samples, which are then taken to the laboratory for analysis. The results showed that the organic matter content of water at high tide was 17.38-25.28 mg/L and at low tide, it was 29.63-35.55 mg/L. The organic matter content of sediments at high tide conditions ranged from 2.28-5.37% and at low tide, conditions ranged from 3.46-8.62%. These conditions indicate that the organic matter content of water and sediment has not passed the predetermined threshold. The results of the analysis of the relationship between the organic matter content of water and sediment at high tide conditions with a coefficient of determination (R2) of 0.533 with a correlation coefficient (r) of 0.730 and at low tide with a coefficient of determination (R2) of 0.713 with a correlation coefficient (r) of 0.844. The type of sediment that dominates the waters of the East Coast of Bengkalis Island is mud.
ANALYSIS OF COASTLINE CHANGES IN RUPAT ISLAND USING REMOTE SENSING DATA AND GEOGRAPHIC INFORMATION SYSTEMS Puspita, Devi; Mubarak, Mubarak; Nursyirwani, Nursyirwani
Asian Journal of Aquatic Sciences Vol. 4 No. 3 (2021): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study was conducted from July to August 2021 in coastal areas of Rupat Island, Riau Province. Coastal zone is an unstable area toward change, because it is a rendezvous place for land and sea, which the line is called shoreline. The purpose of this study was to determine the distance of shoreline change and to find out the landcover change in the coastal area. Method used in this study is a shoreline overlay from Landsat 5 in 1988, 1996, 2004 and Landsat 8 OLI in 2012 and 2020 and classifiation of land cover using supervised classification and Rupat Island monogram. This research showed shoreline changes between 1988 and 2020, abrasion of 106,55 hectares and accretion of 58,28 hectares. The largest land cover change was the decrease of dryland agriculture and forest area of 1530.80 hectares and 726.92 hectares. The largest addition of area occurred on the plantation that is equal to 741.02 hectares.
ABILITY OF AMILOLYTIC BACTERIA (Bacillus paramycoides AND Enterobacter cloacae) IN DEGRADING ORGANIC MATERIALS OF MANGROVE LITTLE Putri, Redila Aprilivia; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol. 4 No. 2 (2021): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aims to find out that Bacillus paramycoides and Enterobacter cloacae bacteria can produce amylase enzymes and have the ability to degrade organic matter, especially mangrove litter. From this study it was found that the optimal growth of B.paramycoides and E. cloacae bacteria occurred at 12th hour. The results of measurements and calculations of absorbance values ​​at 630 10.238 x 108 cells/mL (B. paramycoides) and 12.030 x 108 cells/mL (E. cloacae) using the spectrophotometric method. Meanwhile, with the TPC method at 12 hours, the number of bacterial cells was 2.08 x 108 CFU's/mL (B.paramycoides) and 2.44 x 108 CFU's/mL (E. cloacae). The ability to produce the largest amylolytic bacterial amylase enzyme also occurred at 12 hours as much as 0.306 mg/mL (B.paramycoides) with an increase of 0.046 mg/mL and 0.243 mg/mL (E. cloacae) with an increase of 0.028 mg/mL. The bacteria that have the highest amylase enzyme ability is E.cloacae as evidenced by the diameter of the clear zone of 10.10 mm. Testing the ability of amylolytic bacteria in degrading mangrove litter was carried out by adding amylase enzyme as much as 0%, 50% and 100%. Amylolytic bacteria can degrade organic matter by hydrolyzing starch contained in mangrove litter. The most degraded starch content was in the 100% enzyme treatment, which was 1.021 mg/mL (B. paramycoides) and 1.189 mg/mL (E.cloacae).
UTILIZATION OF MICROALGAE Scenedesmus vacuolatus AS A PHOSPHATE (PO43-) BIOREMEDIATOR IN DOMESTIC WASTEWATER Fathoni, Ahmad Yusron; Nursyirwani, Nursyirwani; Effendi, Irwan; Ambarsari, Hanies
Asian Journal of Aquatic Sciences Vol. 3 No. 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in October 2019 to determine the effect of differences in the addition of KH2PO4 concentrations and the concentration of microalgae Scendesmus vacuolatus with experiment method. The results showed significant effect was found on differences in microalgae concentration and KH2PO4 concentration. KH2PO4 with the concentration of 8 ppm is the most influential on decreasing phosphate levels than KH2PO4 with 5 ppm. The highest samples in phosphate reduction were A2, B2 and C2 with different algal formulations and 8 ppm KH2PO4 concentration. Two-way ANOVA test showed sig. 0.000, Which was mean that there were significant effect to decrease levels of phosphate based on concentration. Post hoc DMRT which has a significantly different dominance value of KH2PO4 concentration results that A1, B1, C1 with A2, B2, C2 have significant differences in the decrease in phosphate levels due to differences in KH2PO4 levels.
EFFECT OF UREA CONCENTRATION ON THE GROWTH OF CELLS OF Nannochloropsis sp. LABORATORY SCALE Wahyuni, Indri; Nurrachmi, Irvina; Nursyirwani, Nursyirwani
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

ABSTRACT This research was conducted from October 2021 to January 2022 in the Natural Feed Laboratory of the Brackish Water Cultivation Fisheries Center (BPBAP) Ujung Batee, Aceh. This study aimed to determine whether urea has an effect and the best urea concentration on the growth of Nannochloropsis sp. This study used an experimental method with one-factor RAL (Completely Randomised Design), consisting of 4 levels of treatment and control with three replications so that 15 experimental units were obtained. The treatments tested were the uses of urea 40 ppm (treatment A), urea 60 ppm (treatment B), urea 80 ppm (treatment C), urea 100 ppm (treatment D), and Walne 1 ml (control). The population density of Nannochlropsis sp. was found in treatment D on day 4 (2414 x 104 cells/mL), and the lowest population growth was shown in treatment A (1177 x 104 cells/mL). The highest absolute growth rate occurred in treatment D (2229x 104 cells/mL), while the highest relative growth rate occurred in treatment D (1205%), and the highest specific growth rate occurred in treatment B (79% per day).
CONCENTRATION OF WATER POLLUTION INDICATORS BACTERIA IN SIRIH PADANG SUMATERA BARAT Ferdana, Gilang; Nursyirwani, Nursyirwani; Amin, Bintal
Asian Journal of Aquatic Sciences Vol. 4 No. 1 (2021): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in October 2019 in the waters of Sirih Bay Padang, West Sumatra Province. The objective of this research aimed was to determine the number of bacterial cells as an indicator of water pollution (total coliform and Escherichia coli) in the waters of Sirih Bay, Padang. The method used in this study was a survey method. Polluted waters in Sirih Bay as sampling location was divided into 3 stations, namely the PLTU area (St. I), near BPBALP Padang (St. II) and a residential area (St. III). Isolation of total coliform and fecal coliform bacteria was carried out by assumption and affirmation tests which were further identified and carried out physiological and biochemical properties. The results showed the total number of coliforms in Sirih Bay waters in the assertion test using BGLB media ranged from 75-460 MPN/ml. This indicates the existence of pollution of coliform bacteria in these waters based on the Decree of the State Minister of Environment No. 51 of 2004 concerning Sea Water Quality Standards, the maximum level of total coliform bacteria is 1000 MPN / 100 ml. The results of this study indicated that the waters of Sirih Bay are considered to be in poor condition
OPTIMIZATION THE GROWTH OF PROTEOLYTIC BACTERIA Bacillus toyonensis AT DIFFERENT SALINITY AND CONCENTRATION OF TOFU WASTEWATER Oktavia, Dewi Asmiati; Nursyirwani, Nursyirwani; Yoswaty, Dessy
Asian Journal of Aquatic Sciences Vol. 5 No. 2 (2022): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Proteolythic bacteria, including Bacillus toyonensis, have potencial to be used in feed industry and in processing of industrial waste. Cultivation of this bacteria requires appropriate enviromental condition and substrate for the growth. The objective of this research was to observe the growth of B. toyonensis at different salinity and tofu wastewater concentration. This experimental research used completely randomized design consisted of two factors. Those were salinity (0‰, 15‰, dan 30‰) and different concentrations of tofu wastewater (8%, 10%, and 12%). Bacterial cell growth was observed by the spectrophotometric method and total plate count (TPC) method. The bacterial cell growth was observed at 0, 24, 48, 72 and 96 hours-incubation. The results indicated that the best medium for the growth B. toyonensis was in tofu wastewater concentration of 12% at salinity of 15‰. The optimum growth occurred at 48 hours-incubation.
Co-Authors ', Afrimansyah A.E.T.H. Wahyuni A.E.T.H. Wahyuni Afrimansyah ' Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Alfarisy, M. Phyto Alfiati Silfi Andhini, Nadia Andrei Putra Zirma Anisa Qoriman Apriliani Indrawati Putri Aras Mulyadi Arman Effendi Asri, Zainul B.A. Moestomo Bachar, Sutan Barbara, Shanta Batubara, Ummi Mardhiah Bintal Amin BP, Valentina Dian Christine M Situmeang Claudia, Kristiwany Mayneke Darmawan, Ernanda P. Desi Anggreni Dessy Yoswati Dessy Yoswaty Desy Mutia Sari Devi Puspita Dewita Dewita Efriyeldi, Efriyeldi Elfis Suanto Elizal, Elizal Elkana Gabariel Fachry Abda El Rahman Fakhrurozi, Fakhrurozi Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Feliatra, Pamela Isya Ferdana, Gilang Fiki Harjuni Furqan, M. Alief Gavion Roston Sitepu Gina Ulfa Fitria Gunova, M Taufik Gunova, M. Taufik Gustina, Julia Gustina, Lenny Halima, Asyatul Hanies Ambarsari Hanifa, Zurya Haura, Sari R. Al Ida Rahayu Simanjuntak, Ida Rahayu Iesje Lukistyowati Indri Wahyuni Irvina Nurachmi Irwan Effendi Irwan Irwan Joko Samiaji Julians, Rizky Fajar Kathy Copper Amolle Lazuardi Lazuardi Lazuardi Umar Lestari, Anggi Lola Pitaloka br Barus Mahdiyah, Evfi Mahmudah, Dewi A. Mardalisa, Mardalisa Meiji Nurdiansyah Miptahul Huda Wassyukur Monika, Siska Mubarak Mubarak Mubarak Mubarak Muhammad Hafiz Perdana Muhammad Hafizh Muhammad Razali Mursawal, Asri Musrifin Galib Nainggolan, Robby Netti Aryani Nur Azizah Nursyafni Nanggola Oktavia, Dewi Asmiati Pakpahan, Deni Panjaitan, Grace P Povy Hutabarat Pratama, Bima Purba, Paedo Yayaki Puspita, Devi Putra, Rajiv R. Putri Ayu Putri, Redila Putri, Redila Aprilivia Putri, Resty Fauziah Rahmanda, Melysa Ramadhan, Ragiel Rinno Ferryno Roiman Pakpahan Ronal Kurniawan Saharudin Saharudin Sarbaini ' Sari, Desy Mutia Shaumi, Nurul Silitonga, Lamtiur Rotua Sinuraya, Tri Setia Darma Sophia Rahmah Saleha Sri Melinda Citra Sri Wahyuni Sufaidah, Siti Nur Suparmi Suparmi Syafri Syafri Syafruddin Nasution Syahada, Melania Syahril Nedi Syahrul Syahrul Thamrin Thamrin Thamrin Thamrin Tjipto Leksono Triyanto . Triyanto Triyanto Triyanto Triyanto Ummi Mardiah Batubara Usman Muhammad Tang Utami, Rahma I. Valentina Dian W. Asmara W. Asmara Widantari, Meilya Widya Asmara Windarti Windarti Wiya, Siti Ada WULANDARI, APRILIA Yahya Benardus Sihombing Yanti, Della Hijri Yoswati, Dessy Yulhadis Yulhadis Yulinda, Three Yuni Fitria Yurestira, Ilham Yusni Ikhwan Siregar Zebua, Abdi Hikmat Petra Zevira, Bella Permata Zhilalul Huda Zulkifli Zulkifli