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All Journal Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Lingkungan Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Abdimas Indonesian Journal Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Jurnal Perikanan dan Kelautan Jurnal Natur Indonesia
Nursyirwani, Nursyirwani
Universitas Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Veterinary Other

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ISOLATION OF DIESEL OIL DEGRADATION BACTERIA IN THE WATERS AROUND THE PORT OF TELUK BAYUR PADANG CITY Sri Melinda Citra; Dessy Yoswaty; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Diesel oil degradation bacteria can be found in various habitats in the marine environment including in the waters around the harbor. This research aims at diesel oil in the waters around the port of Teluk Bayur and test its ability to degrade diesel oil. Isolation of the degrading bacteria has been from April to conductedJune 2018 in the Marine Microbiology Laboratory, Department of Marine Science, Faculty of Fishery and Marine University of Riau.Total count of diesel oil decomposing bacteria ranges from 0.4 x 106 to 7.3 x 106 CFU/ml. Eleven isolates of the diesel oil degradation bacteria were selected, ie BM1a, BM1b, BM1c, BM1d, BM7a, BM7b, BM7c, BM7d, BM14a, BM14c and BM14d. Based on morphological, physiology and biochemical characteristics, seven isolates were irregular, two round and concentric isolates, seven isolates with corrugated colony edges, two irregular and notched isolates, six yellow colony isolates, five white isolates, colony elevation all isolates emerge, all isolates were positive on catalase test, in H2S test all negative isolates, all isolates in the sugar test produced gas, all isolates were motile and Gram negative, all indole isolates were negative, seven methyl red positive isolates and four methy red isolates negative. In the degradation test only six bacterial isolates were selected, ie BM1a, BM1c, BM7a, BM7d, BM14c and BM14d. The highest degradation of diesel oil at concentrations 1%, 2% and 3% were performed by isolates BM1c, BM7a and BM1a respectively. Overall, all isolates were able to degrade diesel oil in the waters around Teluk Bayur Port, Padang City. Keyword: Isolation, Bacteria, Degradation, Diesel Oil 
IS OLATION OF PROBIOTIC CANDIDATE BACTERIA FROM GIANT GOURAMY (Osphronemus gouramy Lac.) INTESTINE TO CONTROL Aeromonas hydrophila Lola Pitaloka br Barus; Iesje Lukistyowati; Nursyirwani '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study aimed to isolate and to find the bacteria that potential as probiotic candidate from Giant Gouramy (Osphronemus gouramy Lac.) intestine cultivated in Riau provincial to be used to control MAS (Motile Aeromonas Septicemia) desease. Survey method and experiment method were conducted in this study. Bacteria was isolated from the fish intestine, and was inoculated on TSA and MRS agar at different pH level (2,4, and 6). The isolateis were indentified by morphological observation, physiological and biochemical test, and by using API 50 CHL test kit. Antibacterial activity of the isolated against Aeromonas hydrophila was tested by the paper disk diffusion method. Eighteen isolates of probiotic candidates were found, and indicated optimal growth in all pH treatment. Based on morphological, physiological test, and biochemichals test, three genus of bacteria were identified as Bacillus sp., Lactobacillus sp., and Bifidobacterium sp. The highest antibacterial activity against A. hydrophila was indicated by Isolate G63 with inhibition zone of 13,2 mm. Based API 50 CHL test kit the isolate was categorized in to Lactobacillus planatarum at similarity level of 84%.Keywords: Giant Gouramy, intestine, isolate, probiotic candidate
EFEKTIVITAS BAKTERI PROBIOTIK DARI UDANG GALAH (Macrobrachium rosenbergii, de Man) TERHADAP BAKTERI PATOGEN Aeromonas hydrophila, Pseudomonas stutzeri DAN Vibrio alginolyticus Desi Anggreni; Feliatra '; Nursyirwani '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractProbiotic bacteria capable of producing substances like antibiotic sandin dispensable for being able to suppressthe growth of pathogenic bacteria into disease and parasites in aquaculture. The research purposeof this study to find probiotic isolates were able to controlthe disease in aquaculture. The candidate probiotic bacteria isolated from prawn (Macrobrachium rosenbergii, de Man) by the method of scratch on TSA and NA media. Anti-bacterial activity using media discs on Zobell and TSA. 4 isolates were found which consisted of coccus, white and orange colonies. All isolates had inhibitory effect onthe growth of pathogenic bacteria. The highest sensitivitytest is indicated by P4 DUG isolates of A.hydrophilla at13,30, 13,20 and14,10 of P.stutzeri in V.alginolyticusKeyword : Probiotic, Effectiveness, Tiger Prawn.
ABILITY OF ISOLATE PROBIOTIC BACTERIA CANDIDATE FROM KAKAP PUTIH (Lates calcarifer) IN PRESSING GROWING OF BACTERI Vibrio alginolyticus IN CO-CULTURE Christine M Situmeang; Nursyirwani '; Irwan Effendi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Three candidates of probiotic bacteria isolated from kakap putih (Lates calcarifer) had been examined for their antagonism against Vibrio alginolyticus. However, the ability to suppress the pathogen in co-culture system has not been tested. This research aimed to test the ability of probiotic candidates to suppress the growth of V. alginolyticus in co-culture trial. Three probiotics candidates (KP1, KP2, and KP3) were used in this experiment method. Four treatments consisted of P1 (broth culture of KP1 + V. alginolyticus), P2 (KP2 + V. alginolyticus ), P3 (KP3 + V. alginolyticus), P4 (V. alginolyticus) as control were prepared in triplicate. Total counts of each probiotic candidate and V. alginolyticus was calculated after 48 hours culture incubation. KP1 isolate showed highest ability suppress the growth of V. alginolyticus, followed by KP3 and KP2 isolates.Keywords: Probiotics, Kakap Putih, Growth, V. alginolyticus, Co-culture
ADHESION OF LACTIC ACID BACTERIA (LAB) TO INTESTINAL EPITHELIAL CELLS OF RED SNAPPER (Lutjanus argentimaculatus) IN INHIBITING Vibrio alginolyticus Gavion Roston Sitepu; Nursyirwani '; Efriyeldi '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The research was conducted from November 2015 until March 2016 in the Marine Microbiology Laboratory and in the Laboratory of Aquaculture Enviromental Quality of in the Faculty of Fishery and Marine Science, Riau University. The purpose of this research was to determine the adhesion ability of Lactic Acid Bacteria (LAB) isolates on intestinal epithelial celss of red snapper (L. argentimaculatus) and in inhibiting adhesion of V. alginolyticus. Completely randomized design (CRD) method was performed in this experimental research. Two factors treated were three LAB isolates (KP 1, KP 2, KP 3 and concertio) and V. alginolyticus. Three adhesion methods applied were competitive, competition and displacement. Adhesion of bacteria to the epithelial cells was calculated based on number of bacterial cells adhered. The results showed that all LAB isolates had higher adhesion ability than V. alginolyticus. Isolate concertio indicated the highest adhesion ability (52.67±7.76 cells/epithelial cells) to the epithelial cells and the lewest was indicated by isolate V. alginolyticus (23.11±2.79 cells/epithelial cells). However, the inhibition ability of the three LAB isolates upon V. alginolyticus by the three adhesion methods was not significantly different.Keywords: Adhesion, Lactic acid bacteria (LAB), V. alginolyticus, Intestinal epithelial cells, red snapper.
Effect of Addition “Attack” Detergent at Different Concentration and Exposure Time on Heterotrophic Bacterial Growth in Sea Water Roiman Pakpahan; Irwan Effendi; Nursyirwani '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research aims to determine the effect of “Attack” detergent addition at different concentration and exposure time on heterotrophic bacterial growth in sea water. The research was conducted from February to April 2017 used experimental method. The results showed that the highest number of heterotrophic bacteria was observed on the A5 day 1st exposure treatment of 6,69 x 107 (CFU/ml) and the lowest was in the 16th day of exposure treatment of 0,098 x 107 (CFU/ml). At day 1st, the value of the correlation coefficient (r) obtained by 0.277 showed the relationship between two variables with positive linearity, while day 6th to day 21th, the correlation coefficient (r) are 0,982, 0,9426, 0,9778 and 0,986, showed the relationship between moderate two was very strong variables with negative linearity. The regression relationship between exposure time and bacterial growth showed a strong correlation with negative linearity, where the coefficient value (r) was 0,87. Significant treatment of detergent concentration that was equal to 0,00 and exposure time that was equal to 0,00 < α (0,05). The bacterial growth on treatment A1 (control) was significantly different with treatment A2, A3, A4 and A5. Bacterial growth at day 1st was significantly different from 6th, 11th, 16th and day 21th. The result of this research showed that there were a effect of “Attack” detergent addition with different concentration and exposure time to the growth of heterotrophic bacteria in seawater.Keyword: Detergent, Concentration, Exposure, Growth, Heterotrophic Bacteria
Identification of Antibiotic-Producing Bacteria from Extreme Microhabitat in Molecular Mangrove Ecosystems and Their Activity on Pathogenic Bacteria (Vibrio alginolyticus) Desy Mutia Sari; Irwan Effendi; Nursyirwani Nursyirwani
Jurnal Perikanan dan Kelautan Vol 9, No 2 (2019)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v9i2.8628

Abstract

The presence of antibiotic-producing bacteria from microhabitat in mangrove ecosystems can be useful to produce new antibiotics for industrial and environmental conservation. Mangrove ecosystems in Dumai have the potential to possess antibiotic-producing bacteria that have not been much studied. The objective of this research was to obtain antibiotic-producing bacteria from extreme microhabitats in the mangrove ecosystem in suppressing the growth of pathogenic bacterial pathogens (Vibrio alginolyticus). Samples were taken from several microhabitat mangrove areas in Dumai (Kelurahan Purnama Dumai), Riau Province. The survey method is used for identification, antibacterial test against pathogenic bacteria (V. alginolyticus). Bacterial isolation was carried out using the pour plate method and purification was carried out using the quadrant streak method. Identification is carried out through observations of macroscopic, microscopic morphology, biochemical tests, antagonism tests (agar diffusion method on agar nutrient media) and DNA analysis. Sequencing of 16S rDNA from the best antibiotic-producing bacterial isolates indicated that molecularly identified of Bacillus amyloliquefaciens, Bacillus cereus, Enterobacter hormaechei, Klebsiella pneumonia, and Enterococcus gallinarum. All bacteria were able to inhibit the growth of V. alginolyticus
kemampuan biodegradasi bakteri selulolitik pada ekosistem mangrove Fiki Harjuni; Nursyirwani .; Irwan Effendi
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 8, No 1 (2020): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.835 KB) | DOI: 10.29406/jr.v8i1.1519

Abstract

Bakteri selulolitik merupakan bakteri yang memiliki kemampuan menghidrolisis kompleks selulosa menjadi oligosakarida yang lebih kecil dan akhirnya menjadi glukosa dimana bakteri selulolitik telah banyak dikaji potensinya dalam memproduksi enzim selulase yang telah dimanfaatkan dalam berbagai bidang, seperti pada bidang pertanian, perikanan, industri dan kedokteran . Tujuan dari penelitian ini adalah untuk mendapatkan miroba selulolitik, mengetahui waktu optimum produksi enzim selulolitik dan bagaimana kemampuan degradasi bakteri selulolitik terpilih terhadap serasah mangrove. Penelitian ini dilaksanakan pada bulan Februari sampai Juni 2019 di Laboratorium Mirobiologi Laut Fakultas Perikanan dan Kelautan Universitas Riau. Metode yang digunakan dalam penelitian ini adalah menggunakan metode survei dan dilanjutkan dengan analisis di laboratorium.  Data diperoleh melalui pengamatan dan pengukuran parameter lingkungan di lapangan, dan analisis sampel di laboratorium.Hasil penelitian menunjukkan bahwa isolasi, seleksi (Identifikasi mikroba pada sedimen hutan mangrove Stasiun Kelautan Dumai), diperoleh 24 isolat yang memiliki kemampuan tumbuh dalam media yang mengandung CMC dengan aktifitas seluloliti tertinggi terdapat pada isolat BS.ST2.8. Hasil karakteristik isolat bakteri selulolitik secara fenotip dan genotip diketahui bahwa ketiga isolat tersebut adalah B. toyonensis. Enzim selulase terbanyak dihasilkan oleh isolat bakteri BS.ST2.8 terjadi pada jam ke -78 setelah inkubasi. Dari hasil uji lebih lanjut dengan menggunakan beberapa dosis enzim selulase isolat BS.ST2.8 terhadap substrat serasah mangrove diketahui bahwa secara umum dapat mendegradasi serat kasar pada serasah mangrove pada dosis 50%  penurunan fraksi serat NDF, ADF, selulosa dan hemiselulosa.
ANALYSIS OF COASTLINE CHANGES IN RUPAT ISLAND USING REMOTE SENSING DATA AND GEOGRAPHIC INFORMATION SYSTEMS Devi Puspita; Mubarak Mubarak; Nursyirwani Nursyirwani
Asian Journal of Aquatic Sciences Vol 4 No 3 (2021): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.4.3.236-246

Abstract

This study was conducted from July to August 2021 in coastal areas of Rupat Island, Riau Province. Coastal zone is an unstable area toward change, because it is a rendezvous place for land and sea, which the line is called shoreline. The purpose of this study was to determine the distance of shoreline change and to find out the landcover change in the coastal area. Method used in this study is a shoreline overlay from Landsat 5 in 1988, 1996, 2004 and Landsat 8 OLI in 2012 and 2020 and classifiation of land cover using supervised classification and Rupat Island monogram. This research showed shoreline changes between 1988 and 2020, abrasion of 106,55 hectares and accretion of 58,28 hectares. The largest land cover change was the decrease of dryland agriculture and forest area of 1530.80 hectares and 726.92 hectares. The largest addition of area occurred on the plantation that is equal to 741.02 hectares
MANGROVE LITTER DECOMPOSITION SPEED IN MARINE STATION DUMAI Nur Azizah; Joko Samiaji; Nursyirwani &#039;
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study of was conducted in December 2014 - March 2015 in the area of mangrove forests Marine Station Dumai. The purpose of this study was to determine the speed of litter decomposition of mangrove and the diversity of fungi in litter of mangrove. The method used was survey method. The sampling and measurement for water quality included salinity, temperature and pH that were done directly in the field. Weighing litter carried out at the Laboratory of Marine Chemistry whereas litter decomposition data analysis and identification of fungi diversity was carried out in the Laboratory of Marine Microbiology Faculty of Fisheries and Marine Sciences University of Riau. Mangrove leaf litter decomposition was in the highest at station III with a percentage value of litter decomposition rate of 1.31% per day. The identification results in leaf litter on the mangrove areas showed that there were eight types of fungi that included two of the species Aspergillus sp., Chaetomium sp., Candida sp., Gliocladium sp., Culvularia sp., Bipolaris sp. and Exophiala sp. The most often fungi found in each observation was Gliocladium sp. The highest fungi species diversity index was at score of 2.08 on a mangrove leaf litter that was found on observation IV.Key words : mangrove , litter , decomposition rate , fungi , Dumai Marine Station
Co-Authors ', Afrimansyah A.E.T.H. Wahyuni A.E.T.H. Wahyuni Afrimansyah &#039; Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Alfarisy, M. Phyto Alfiati Silfi Andhini, Nadia Andrei Putra Zirma Anisa Qoriman Apriliani Indrawati Putri Aras Mulyadi Arman Effendi Asri, Zainul B.A. Moestomo Bachar, Sutan Barbara, Shanta Batubara, Ummi Mardhiah Bintal Amin BP, Valentina Dian Christine M Situmeang Claudia, Kristiwany Mayneke Darmawan, Ernanda P. Desi Anggreni Dessy Yoswati Dessy Yoswaty Desy Mutia Sari Devi Puspita Dewita Dewita Efriyeldi, Efriyeldi Elfis Suanto Elizal, Elizal Elkana Gabariel Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Feliatra, Pamela Isya Ferdana, Gilang Fiki Harjuni Furqan, M. Alief Gavion Roston Sitepu Gina Ulfa Fitria Gunova, M Taufik Gunova, M. Taufik Gustina, Julia Gustina, Lenny Halima, Asyatul Hanies Ambarsari Hanifa, Zurya Haura, Sari R. Al Ida Rahayu Simanjuntak, Ida Rahayu Iesje Lukistyowati Indri Wahyuni Irvina Nurachmi Irwan Effendi Irwan Irwan Joko Samiaji Julians, Rizky Fajar Kathy Copper Amolle Lazuardi Lazuardi Lazuardi Umar Lestari, Anggi Lola Pitaloka br Barus Mahdiyah, Evfi Mahmudah, Dewi A. Mardalisa, Mardalisa Meiji Nurdiansyah Miptahul Huda Wassyukur Monika, Siska Mubarak Mubarak Mubarak Mubarak Muhammad Hafiz Perdana Muhammad Hafizh Muhammad Razali Mursawal, Asri Musrifin Galib Nainggolan, Robby Netti Aryani Nur Azizah Nuri Aslami Nursyafni Nanggola Oktavia, Dewi Asmiati Pakpahan, Deni Panjaitan, Grace P Povy Hutabarat Pratama, Bima Purba, Paedo Yayaki Puspita, Devi Putra, Rajiv R. Putri Ayu Putri, Redila Putri, Redila Aprilivia Putri, Resty Fauziah Rahmanda, Melysa Ramadhan, Ragiel Rinno Ferryno Roiman Pakpahan Ronal Kurniawan Saharudin Saharudin Sarbaini &#039; Sari, Desy Mutia Shaumi, Nurul Silitonga, Lamtiur Rotua Sinuraya, Tri Setia Darma Sophia Rahmah Saleha Sri Melinda Citra Sri Wahyuni Sufaidah, Siti Nur Suparmi Suparmi Syafri Syafri Syafruddin Nasution Syahada, Melania Syahril Nedi Syahrul Syahrul Thamrin Thamrin Thamrin Thamrin Tjipto Leksono Triyanto . Triyanto Triyanto Triyanto Triyanto Ummi Mardiah Batubara Usman Muhammad Tang Utami, Rahma I. Valentina Dian W. Asmara W. Asmara Widantari, Meilya Widya Asmara Windarti Windarti Wiya, Siti Ada WULANDARI, APRILIA Yahya Benardus Sihombing Yanti, Della Hijri Yoswati, Dessy Yulhadis Yulhadis Yuni Fitria Yurestira, Ilham Yusni Ikhwan Siregar Zebua, Abdi Hikmat Petra Zevira, Bella Permata Zhilalul Huda