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All Journal Jurnal Ilmu Kimia Universitas Brawijaya KOVALEN: Jurnal Riset Kimia Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Adi Widya : Jurnal Pengabdian Masyarakat CHEESA: Chemical Engineering Research Articles JOPS (Journal Of Pharmacy and Science) Jurnal Surya Masyarakat Klinikal Sains (Jurnal Analis Kesehatan) Surya Abdimas Jurnal Biomedika INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang JURNAL ANALIS LABORATORIUM MEDIK Community Service Journal of Indonesia Jurnal Ilmiah Farmako Bahari Medicra (Journal of Medical Laboratory Science/Technology) Jurnal Surya Masyarakat Indonesian Journal of Cultural and Community Development Borneo Journal Of Pharmascientech Environmental and Toxicology Management (ETM) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis SWARNA Journal of Artificial Intelligence and Digital Business Cerdika: Jurnal Ilmiah Indonesia Journal of Pharmacy Science and Technology Jurnal Laboratorium Khatulistiwa SWARNA: Jurnal Pengabdian Kepada Masyarakat
Ersalina Nidianti
Universitas Nahdlatul Ulama Surabaya, Indonesia
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Journal : Jurnal Biomedika

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Cytotoxicity Assay of Chitosan-Collagen Wound Dressing using Brine Shrimp Lethality Test Methods Ary Andini; Ersalina Nidianti; Endah Prayekti
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.680

Abstract

Wound dressing has a function to protect the wound area from external exposure and provide a moist state around the wound area for accelerating wound healing process. Collagen and chitosan are known as appropriate biomaterials to synthesise of wound dressing because they have anti-bacterial, anti-fungal, biodegradable and biocompatible properties. One of biocompatibility assay for a material is cytotoxicity assay using Brine Shrimp Lethality Test (BSLT) that could be applied before in Vivo assay. The aim of the research was to know the cytotoxicity level of collagen-chitosan wound dressing with variance of concentration such as K0 as control used pure chitosan, K1 used collagen 25% and chitosan 75%, K2 used collagen 50% and chitosan 50%, K3 used collagen 75% and Chitosan 25%. Skin and scales of Gabus fish (Channa striata) were extracted using 2% HCl solvent to obtain collagen and chitosan powder dissolved in 1% acetic acid. Furthermore, wound dressing is made by a combination of collagen-chitosan concentration according to each group (K0, K1, K2 and K3. Citotoxicity assay used Brine Shrimp Level Test (BSLT) method with concentration each sample group were 10, 50, 100, 250, 500, 750 and 1000 ppm. The results showed that each wound dressing group such as K0, K1, K2 and K3 had LC50> 1000ppm that indicated wound dressing was non-toxic.
Co-Authors Adinda Priyanti Dewi Afianti Diah Anjelia Aidilfi Akbar, Dimas Arendra Aini, Fadlilah Nur Aisah Humairoh Aisyah Haddad, Fitri Algristian, Hafid Andini Devi Rizkiyah andini, ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ary Andini Ary Andini Aulia, Ilmiah Alvi Nisa Cahyani, Yuliana Dwi Kurnia Chika Nur Azizah Dea Ayu Rahmawati Desi Susanti Devyana Dyah Wulandari Devyana Dyah Wulandari Dewi Masithah, Dewi Endah Budi Permana Putri Endah Prayekti Endah Prayekti Fadlilah Nur Aini Farah Nuriannisa Fitri Aisyah Haddad Gilang Nugraha Halimah Prisilia Herliani Rahmania Sari Hidayat, Muhammad Taufiq Himawan, Toto Ilmiah Alvi Nisa Aulia Intan Oktariza Nuraulya Putri Irmawati Irmawati KARIMAH, AKHLAQUL Kartika Sari, Nathalya Dwi Khusnah, Hidayatul Kombih, Muhammad Fifin Kurniasari, Diyan Wahyu Maat, Suprapto Mardhotillah, Rachma Rizqina Ma’at, Suprapto Miladiya, Feby Ayu Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Nandasari, Anggy Dwipa Ngiluhtara Aditiya Putri Nila Dwi Utami Nisrina Salsabila Firdaus Nurdinata, Sandra Maulida Nuris Lutfika Nurul Hidayatih Pertiwi, Penti Prayekti, Endah Puspitasari, Fike Putri, Bismi Aisyah Rahma Putri, Ngiluhtara Aditiya Qotrinnada, Nabilah Rahayu Anggraini Rahayu Anggraini Rahmawati Fitri Awalia Rizka Amalia Rizka Amalia Rizkiyah, Mubarokator Rohmah, Wardah Romdloni, Muhmmad Afwan Rukman, Nia Kurniaty Safitri, Hana Salfa Salsabilah Zain Salfa Salsabilah Zain Santoso, Rizma Defva Sari, Herliani Rahmania Sari, Yuni Nur Malita Saumia Khoirunnisa Syadzila Septyanengrum, Rita Siska Wulandari, Siska Siti Basiroh Sri Surya Suciati Suciati, Sri Surya Sunaryo, Merry Suprapto Ma’at Syadzila, Saumia Khoirunnisa Syiddatul Budury Utami, Nila Dwi Utomo, Edi Priyo Widya Putra, Bima Arya Wieke Sri Wulan Wieke Sri Wulan Wulan, Wieke Sri Yauwan Tobing Lukiyono Yuliani, Kartika Yuni Nur Malita Sari Zain, Salfa Salsabilah