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Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Azura, Sarah; Ratnani, Hermin; Susilowati, Suherni; Hariadi, Mas'ud; Samik, Abdul; Soepranianondo, Koesnoto
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (923.396 KB) | DOI: 10.20473/ovz.v9i1.2020.1-6

Abstract

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.
Serum Progesterone profile of pregnant Ettawa crossbred does at day-0, -21, -42, -63 and -84 Bellani, Ayu Aprodhita Cintya; Ismudiono, Ismudiono; Hariadi, Mas'ud; Lokapirnasari, Widya Paramita
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.53-58

Abstract

This study aimed to determine the progesterone concentrations of Ettawa crossbred does at day-0, -21, -42, -63, and -84 after mating. Five Ettawa crossbred does were injected with PGF2α twice with an interval of 11 days for estrous synchronization followed by natural mating on day-3 after the second PGF2α injection. Blood was collected through the jugular vein for the measurement of serum progesterone concentrations using ELISA, whereas pregnancy was diagnosed at 90 days after mating by abdominal palpation. The results showed that all does were on estrus onthethird day after the second PGF2α, three of them diagnosed to be pregnant following mating. The progesterone concentration at day-0 was 1.52 ± 0.33 ng/mL, increased (p <0.05) to 7.08 ± 1.02 and 5.50 ± 2.82 ng/mL at day-21 and day-42, and increased (p <0.05) again to 12.01 ± 5.30 and 12.04 ± 0.30 ng/mL at day 63 and day 84, respectively. There was no significant difference in progesterone concentrations between day 21 and day 42 and between day 63 and day 84. It could be concluded that there was a biphasic increase of progesterone concentrations of the pregnant Ettawa crossbred does, i.e. from day-0 to day-21/day-42 and from day-21/day-42 to day-63/day-84. 
Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows Ulfah, Norma Amalia; Samik, Abdul; Hariadi, Mas'ud; Suprayogi, Tri Wahyu; Hidanah, Sri
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.9-14

Abstract

Small farmers dominate dairy farming in Indonesia, and the average productivity is low. The contribution of domestic production only reaches about 23% of the total national milk consumption needs as a source of human nutrition. This study was aimed to identify the relationship between cow's parity and  age to services per conception (S/C), conception rate (CR), and gestation length (GL) of Holstein Friesian (HF) cross dairy cows. Data of this study consisted of primary data obtained from farmers interview, and secondary data from the Cooperative Unit of Argopuro village, Krucil, Probolinggo. Sample size was 200 cows, which was 10% of the total population. Data were analyzed for correlation and linear regression using the Statistic Package and Service Solution (SPSS) software version 20 (IBM Corp., USA) at a 95% confidence level. The average S/C, CR, and GL were 2.55 ± 1.57, 21%, and 278.19 ± 11.92 days, respectively. There was no significant correlation (p >0.05) between the parity with S/C, CR, and GL and the age with CR and GL. The only significant correlation (p <0.05) was between the age and S/C. Regression of S/C based on age followed the equation S/C = 0.104 + 0.407*age, with a correlation coefficient and determination coefficient of 0.733 and 0.538, respectively. It could be concluded that S/C could be predicted based on the age of the cows.
The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer's-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster Pitaloka, Dinda Galuh; Mas'ud Hariadi; Suherni Susilowati; Budi Utomo; Tatik Hernawati; Eka Pramyrtha Hestianah; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.49-58

Abstract

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer's-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours.
Penggemukan sapi menggunakan pakan tanpa hijauan (complete feed) di era pandemi covid-19 (Fattening of cow cattle using green-free feed (complete feed) in the covid-19 pandemic era) Meles, Dewa Ketut; Mustofa, Imam; Wurlina, W; Hariadi, Masud
Buletin Pengabdian Vol 1, No 2 (2021): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v1i2.21640

Abstract

Problems in the COVID-19 pandemic era for cows cattle farmers were limited skills of farmers in processing of feed without forage in order to reduce production costs and anticipate prolonged droughts. The government's steps to overcome these problems are by providing business capital assistance, deferring loan payments, and assisting cows cattle farmers. The Regional Superior Product Development Program (RSPD) aims to determine the increase in body weight of various types of cattle using feed without forage and growth promoters in the COVID-19 pandemic era of SMEs of cows cattle fattening. Data was collected by interview/questionnaire methods and making feed without forage, growth promoter, and fermentor. The feed from RSPD was applied to five types of cows cattle (local cattle, ongole crossbreeds, madura cattle, limousin crossbreeds and Simmental crossbreeds). This activity was carried out from February until June 2021. The results showed that farmers skill in making feed without forage, growth promoters, and fermenters has been improved and also type of cows cattle with good weight gain has been determined. In conclusion, the production of feed without forage, growth promoters, and fermentor in the COVID-19 pandemic era could be done by the cattle farmers independently. Limousin and Simmental crossbreeds had good weight gain compared to local cattle, ongole crossbreeds and madura cattle.