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Profil Histopatologi Otot Jantung pada Tikus Hipertensi (Doca-Garam) yang Dipapar Kombinasi Ekstrak Metanolik Benalu Teh Dan Benalu Mangga (EMBTBM) Annisatul Anis Hakimah; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p20

Abstract

Some herbs from the parasite group and the Loranthaceae family also act as antihypertensives. This study aims to look at the histopathological profile of the heart in hypertensive rats (DOCA-Salt) exposed to the combination of Methanolic Extract of Benalu Tea and Mango Benalu (EMBTBM) for 14 days using true experimental design method with completely randomized design on 50 rats, divided into 5 controls -), control (+), treatment 1 dose 50 mg / KgBB, treatment 2 doses 100 mg / KgBB and treatment 3 doses 200 mg / KgBB. Each treatment had 5 replications. In analyzing the data used the ANOVA test, one of the methods is JAMOVI with version 1.1.9.0. As for the calculation of cell diameter using the IMAGE J application. The results showed that the histopathological profile of the heart in hypertensive rats (DOCA-Salt) exposed to EMBTBM for 14 days on heart cell necrosis showed very real evidence between the control (+) and control (- ), treatment 1, 2, and 3. This is evidenced by the results of the analysis p-value <0.05, namely 0.002, so it is denied that EMBTBM has an effect on the average number of necrosis of cardiac cells. There is also a significant difference in the number of heart cell diameter of hypertensive rats between the control (+) and control (-) groups, treatment 1, 2, and 3. This is evidenced by the results of the analysis showing a p-value <0.05, namely 0.021, so that it can it is denied that EMBTBM can shrink the diameter of cells in the heart organ. Keywords: Hypertension, Heart, Left Ventricle, Anatomical Histopathology, EMBTBM.
DNA barcoding pada invertebrata laut Agus Mohammad Hikam; Nurul Jadid Mubarakati; Muhammad Dailami; Abdul Hamid A. Toha
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p06

Abstract

A large number of species of marine invertebrates that have similar morphology cause misidentification very possible. Molecular species identification is important in taxonomy study accurately. This study aims to determine the identity of Aquatic Papua marine invertebrates with DNA barcoding technique using markers COI gene (Cytochrome c oxidase I). The study identified 29 individuals of marine invertebrates, which came from sea cucumbers (6 samples), lobsters (6 samples), octopus (6 samples), chitons (5 samples), and sea urchins (6 samples). Molecular identification methods consist of DNA isolation, PCR COI gene fragments, sequencing, and DNA sequence analysis using bioinformatics software. The results showed that the sea cucumber came from the Bohadschia marmorata species, the lobster came from the Panulirus versicolor species, the octopus came from the Octopus cyanea species, the chiton came from the Ischnochitonaustralis species, and the sea urchins came from the Tripneustesgratilla species. The similarity of samples and references are in the range of 84.58 to 100.00%. The disparity index, genetic distance, and phylogenetic tree supported this result.
KARAKTERISASI PROFIL ZONA PELUSIDA MANUSIA (Homo sapiens) MENGGUNAKAN ANALISIS IN SILICO Nurul Jadid Mubarakati; Saimul Laili
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 2 No 2 (2017): Dunia Makhluk Hidup Mikro dan Makro
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.248 KB)

Abstract

Most important in mammlian fertilization were egg binding sperm, whose fusion generates a zygote that will develop into a new individual. In mammals, zona pellucida (ZP) is a specialized extracellular matrix of the egg. The matrix is important for this process by directly mediating species-restricted recognition between gametes. The aims of this study was to analyze the profile of human (Homo sapiens) zonna pellucida (hZP) using in silico method, position of O-linked and N-linked glycosilation units on human ZP amino acid sequence and to predict interaction between ZP and ADAM 2. The method of research was using in silico analysis. ZP protein profile was using data mining in UNIPROT database. Profile exploration searched primery funtion of protein, location, expression place, protein structure and ZP-interaction protein. The Primer data was compared each other. Determining O-linked and N-linkedglycosilation units in hZP amino acid sequence was analyzed using O and N-Glycosylation sites prediction. The results showed that in silico analysis conducted that profile of ZP3 identified as a primary receptor sites for spermatozoa and induced acrosome reaction. Amino acid sequence in ZP have potential site in N-Linked and O-linked glycosilation units, but have quantity different. Keywords : human zonna pellucida, N- Glycosylation, O- Glycosylation, in silico.ABSTRAKTahapan awal yang paling penting dalam fertilisasi mamalia adalah ikatan antara sel telur dan sperm yang dilanjutkan terbentuknya zigot dan terjadi perkembangan individu baru. Pada mamalia, zona pelusida merupakan lapisan ekstraseluler sel telur. Lapisan ini sangat penting dalam proses pengenalan secara langsung yang dimediasi oleh interaksi gamet. Tujuan dari penelitian ini adalah untuk mengetahui profil zona pelusida manusia secara in silico, untuk mengetahui posisi O-linked dan N-linked pada sekuen ZP manusia (Homo sapiens) serta memprediksi interaksi antara ZP dengan ADAM 2. Metode yang digunakan dalam penelitian ini adalah menggunakan analisis in silico.Profil Protein Zona pelusida dilakukan dengan cara data mining di database UNIPROT. Eksplorasi profil dilakukan pada pencarian fungsi utama protein, lokasi protein, tempat ekspresi, struktur protein,dan juga protein yang berinteraksi dengan zona pelusida. Informasi dasar tersebut kemudian dikomparasi agar diketahui peranan protein yang paling krusial. Penentuan sisi potensial O-linked dan N-linked pada sekuen ZP manusia dianalisis menggunakan O dan N-Glycosylationsites prediction. Interaksi ZP dengan ADAM 2 menggunakan moleculer docking. Hasil penelitian menunjukkan bahwa profil zona pelusida (ZP) secara in silico diperoleh bahwa ZP3 berperan sebagai reseptor primer yang berperan dalam ikatan dengan spermatozoa pada oosit dan menginduksi reaksi akrosom. ZP memiliki sisi potensial pada N-glikosilasi maupun O-glikosilasi, namun memiliki jumlah yang berbeda.Kata kunci: Zona Pelusida manusia, N-glikosilasi, O-glikosilasi, in silico
Studi Subkronik.28 Hari: Uji Toksisitas Ekstrak Metanolik Kombinasi Scurulla atropurpurea dan Dendrophthoe pentandra terhadap Kerusakan Fungsi Ginjal Tikus Wistar Betina Malia Anjani; Nour Athiroh AS; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.141 KB)

Abstract

Mistletoe tea (Scurrula. atropurpurea (Bl.) Dans) and Mistletoe Mango (Dendrophthoe pentandra) are parasitic plants that live on tea and mango plants, very potential as medicines, containing several secondary metabolite compounds such as flavonoids (quercetin). Flavonoids can act as natural antioxidants that can protect the kidneys from free radicals. Damage to kidney function can indicate from creatinine, BUN, urea, and renal histopathology. The.purpose.of.this study was.to determine toxicity of. a combination of. Mistletoe tea. extract and mango mistletoe (EMBTBM) to damage kidney function in female Wistar rats for 28 days, using the true experimental design method. Data on creatinine, bun, urea, and renal histopathology data were analyzed using the ANOVA. test. The number of test animals is 20 female white rats divided into four groups; each group there are five rats. Group.1 a control, then groups 3, and 4. as. treatment. Based on the results of the study showed that statistically significant. between. all groups. was p> 0.05. Therefore,.EMBTBM given to female.rats for.28 days at a dose.of 250 mg/KgBB, 500.mg/KgBB, and.1000 mg/KgBB reduces levels of creatinine, bun, urea and renal cell necrosis, in this case, the administration of EMBTBM tends to be safe and does not cause rise toxic properties in damage to kidney function in female Wistar rats. Keywords: .Subchronic, kidney function, and extract. ABSTRAK Benalu teh (Scurrula atropurpurea (Bl.) Dans) dan Benalu Mangga (Dendrophthoe pentandra) merupakan tanaman parasit yang hidup.menumpang pada tanaman teh dan mangga, sangat berpotensi sebagai obat-obatan, karena mengandung senyawa flavonoid yaitu quercetin dan rutin. Flavonoid berperan sebagai penyedia. antioksidan. alami. yang. melindungi. ginjal dari zat radikal.bebas. Kerusakan.fungsi ginjal diindikasi dari. kadar. kreatinin, BUN, urea, dan histopataologi ginjal. Tujuan penelitian ini adalah mengetahui. toksisitas pemberian. kombinasi ekstrak. benalu. teh. dan benalu mangga (EMBTBM) terhadap kerusakan fungsi ginjal pada tikus wistar betina selama 28 hari, dengan metode true eksperimental design. Data kadar kreatinin, bun, urea, dan histopatologi ginjal dianalisis menggunakan uji ANOVA. Penelitian ini menggunakan hewan.coba.berjumlah. 20. ekor. tikus. putih. betina. Hewan coba dibagi. menjadi 4.kelompok denga 5 ekor tikus pada tiap kelompok. Kelompok.1sebagai. kontrol, sementara kelompok. 3 dan 4 diberi perlakuan EMBTBM. Hasil. penelitian. menunjukan bahwa. perbedaan. nilai signifikan. antara. semua. kelompok. yaitu p>0.05..EMBTBM yang disondekan pada tikus wistar betina.selama 28.hari. dengan. dosis yang diberikan yaitu 250. mg/KgBB, 500. mg/KgB, dan 1000. mg/KgBB menurunkan kadar kreatinin, bun, urea dan nekrosis sel ginjal, dalam hal ini pemberian EMBTBM cenderung aman dan tidak menimbulkan sifat toksik pada kerusakan fungsi ginjal tikus wistar betina. Kata Kunci. : Ekstrak, Fungsi ginjal, dan Subkronik.
Uji Toksisitas Subkronik 28 Hari Kombinasi Ekstrak Metanolik Benalu Teh dan Benalu Mangga Terhadap Profil Protein pada Tikus Wistar (Rattus norvegicus) Betina Umu Intan Kinasih; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.375 KB)

Abstract

There are 3 indicators for indicating a protein breakdown in the blood through a total protein, albumin, and globulin stability. A compound capable of acting as antioxidants is needed to stabilize the protein's condition. This research is aimed at identifying the toxic effects of extract’s combination from tea parasite and mango parasite on the conditions of blood’s protein by giving EMBTBM constantly for 28 days. The data analysis used ANOVA one-way by SPSS version 17.0. As for the animal test used was a female Wistar rat of 20 with four treatments are control, treatment 1 with a dose of 250mg/Kg body weight (BW). Treatment 2 with a dose of 500mg/Kg body weight (BW) and treatment 3 with a dose of 1000mg/Kg body weight (BW). The result of this research shows that there is no difference between treatment and control over the results of a total protein and albumin. But, at the result of globulin, there is a difference between treatment and control. Therefore, EMBTBM is safe for profile protein and does not cause toxicity because protein is stable and not over-reducing. Keywords:Subchronic,Total Proteins, Albumine, Globuline, Toxicity
Uji Toksistas Subkronik 28 Hari Ekstrak Metanolik kombinasi Daun Benalu Teh dan Benalu Mangga terhadap fungsi Hepar Pada Tikus (Rattus norvegicus) Betina Hatif Khusnin Nida'; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (856.734 KB) | DOI: 10.33474/e-jbst.v7i2.355

Abstract

ABSTRACT Flavonoids and quercetin are metabolite compounds contained in parasite plants. The role of the compounds is as an antioxidant that can protect cell damage from free radicals,for example liver. The presence of antioxidant can neutralize and protect the liver from free radicals. Liver is an organ that is vulnerable to damage caused by drugs or toxic chemicals because the liver is the center of metabolism in the body. Can be known with indicators of increasing level of SGOT, SGPT total bilirubin and histopathologic liver. The purpose of this study to determine the effect of the combination of extracts of tea parasite and mango parasite (EMBTBM) on levels of liver function in female rats (Rattus norvegicus) for 28 days. Data were analyzed using ANOVA version Jamovi 1.0.1.0.. The number of test animals is 20 female rats were divided into four groups, group 1 as a control, group 2,3,4 as a treatment. The treatment groups was given EMBTBM with different doses of 250 mg/kgBW, 500 mg/kgBW and 1000 mg/kgBW. Based on the research results show that a significant value difference between the group was p> 0,05. Therefore EMBTBM given to female rats for 28 days with a dose of 250 mg/BW, 500 mg/kgBW and 1000 mg/kgBW in the treatment group all dose were not significantly different compared with controls. So that the level of liver function in female rats was safe (not toxic) and has no effect on liver histopathology of female wistar rats. Keyword: Subchronic, Extract and liver function
Analisis Distribusi Serangga Tanah Jalan MT Haryono dan Tlogomas Malang Hasan Zayadi; Nurul Jadid Mubarakati
Biota Vol 10 No 2 (2017)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1120.083 KB) | DOI: 10.20414/jb.v10i2.3

Abstract

Vegetation in urban areas can be viewed, as compensation for environmental change due to urbanization of plant diversity affecting the diversity of animals in an ecosystem, the loss of plant species will disrupt the food webs in the ecosystem. Research on the diversity of soil insects found in shade trees species is still not widely done. The purpose of this study were to identify the types of soil insects found in shade trees along Dinoyo and Tlogomas roadside in sub-district of Lowokwaru, Malang and to characterize spatial distribution of soil insect species found in shade trees along Dinoyo and Tlogomas roadside in sub-district of Lowokwaru Malang by using GIS application. Sampling was conducted at two different locations (MT Haryono and Tlogomas Road). Soil insect analysis was calculated using Shannon-Wiener (H') index at different sites. Variables measured were Relative Frequency (FR) and Relative Abundance (KR), while mapping Spatial descriptions. Distribution of soil insects was done by stages in spatial data analysis including digitization, attribute data, map overlay, and map output results. The results of this study obtained the number of species of soil insects as many as 14 species with a total of 287 individuals. Soil insects most commonly found were family Formicidae, the species of Momorium destructor with the number of individuals as much as 87 individuals, and Paratrechina longicornis as many as 86 individuals.
In silico exploration the phenolic compound of olive leaves as acetylcholinesterase enzyme (AChE) inhibitor for Alzheimer’s disease therapy Nurul Jadid Mubarakati; Oktavia Rahayu Puspitarini; Tintrim Rahayu; Alik Maulidiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.905 KB) | DOI: 10.23869/23

Abstract

Olive (Olea europaea) have been cultivated and grown well in tropical climates such as Indonesia. Indonesia local community have used olive as herbal medicines due to its active compounds known as oleuropein that has many biological activities including as neurotherapy in Alzheimer's disease. The purpose of this study is to explore the potential phenolic compounds of olive and examine the acetylcholinesterase (AChE) inhibitory activity displayed by different olive polyphenols through a silico approach. The bioactive compounds of olive which had been analyzed in this study were phenolic compound included oleuropein, demethyl-oleuropein, ligstroside, oleoside, verbascoside, luteolin 7-glucoside, and hydroxytyrosol. Interaction of bioactive compounds with acetylcholinesterase (AChE) was analyzed through molecular specific docking using AutoDock Vina with Pyrx Software. The result elucidate that olive contain potential biological activities as antioxidant, anti-inflammatory, antineoplastic, free radical scavenger, antibacterial, antifungal, expression TP53 enhancer, caspase 8 stimulant, platelet adhesion inhibitor, treatment for lipoprotein disorder, antiviral and dementia treatment/Alzheimer disease. The highest bioactivity percentage of olive phenolic compound are as an antioxidant of 82%, anti-inflammatory of 73%, and anti-cancer (antineoplastic) of 70% respectively. Based on molecular docking analysis show one of olive phenolic compound of the dimethyl-oleuropein has strong interaction with AChE as pointed in the binding affinity of demethyl-oleuropein +AChE of -8.9 kcal/mol has closed to galanthamine binding affinity of -10.3 kcal/mol. Along with glutamate acid 202, tyrosine 133 and tyrosine 124 are the major contributors in the target-ligand interactions. The selected demethyl-oleuropein ought to be tested in clinical studies to discover new neuro-therapeutic candidates.
The portrait of neem leaves-based high performance wound healing activity on zebrafish Nour Athiroh; Ari Hayati; Istirochah Pudjiwati; Ahmad Taufiq; Nurul Jadid Mubarakati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20214

Abstract

This study was designed to evaluate the role of sliced and dropping models of neem leaves (Azadirachta indica) and environmental factors on zebrafish wound shrinkage. This study employed two treatment models: neem leaf slices and drops model. The treatment in the neem leaf slice model was the control group (fish was injured without neem leaf slices treatment, and G1-G3 of each fish was injured at 0.3 cm + 0.5, 1, and 2 g. Treatment in the neem leaf drop model was control group (fish was injured without neem leaves drops, and G1-G3 of each fish was injured at 0.3 cm + 1, 2, 4 drops. Findings suggest that there is a significant difference between the control group by treating G1, G2, and G3 on the shrinkage of zebrafish wound area both in the neem leaf slice and drop models, but G1 was not significantly different from G2 and G3, as well as G2 and G3 both in the neem leaf slice and drop model. Neem leaves contain nutrients that play a vital role in the formation of collagen and the formation of new capillaries to help speed up the wound healing process. The healing process involves the dynamic interaction of physiological factors.
IDENTIFIKASI TOTAL MIKROBA, CEMARAN Escherichia coli DAN NILAI pH SUSU PASTEURISASI YANG BEREDAR DI KOTA MALANG Oktavia Rahayu Puspitarini; Nurul Jadid Mubarakati
JURNAL ILMIAH PETERNAKAN TERPADU Vol 7, No 1 (2019)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.568 KB) | DOI: 10.23960/jipt.v7i1.p201-205

Abstract

Malang is a rich culinary city of livestock products such as pasteurized milk. The purposes of this study were analyzed total microbes, contamination of Escherichia coli and pH of pasteurized milk produced by milk store, village unit cooperation milk house and STMJ cafe in Malang. The method of this research were survey and experiment. The samples were took from milk store (P1), village unit cooperation milk house (P2) and STMJ café (P3)  in Malang during 4 days. The data analysis of total microbial and pH using one-way analysis of variance (ANOVA) and continued by Duncan Multiple Range Test (DMRT) while Escherichia coli data was analyzed by descriptive analysis. The results showed that there were a real influence (P<0,05) of milk producers on total microbes and pH. Escherichia coli contaminant from three producers were showed the same results which were less than 3 apm/ml. The conclusions of this study were total microbes and contamination of Escherichia coli original pasteurized milk produced by milk store, village unit cooperation milk house and STMJ cafe under the limit of microbial contamination on food. Microbiological quality in terms of total microbes, and contamination of Escherichia coli pasteurized milk by milk store in Malang City were classified to national standard of pasteurized milk in Indonesia. Keywords: Total Microbes, Escherichia coli, pH, Pasteurized Milk, Malang City
Co-Authors Abdul Hamid A. Toha Abdullah Rozzen Muzayyan Adi Sofyan Ansori, Muhammad Agus Mohammad Hikam Ahmad Taufiq Alik Maulidiyah Annisatul Anis Hakimah Ari Hayati Arifiati, Shinta Arina Roikhana Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Azis Mawardi Azis Mawardi Chintya Ayu Pertiwi Della Aljanna Savita Devi Indrayani Fitri Asari Dewi Qurrot &#039;Aini Dewi, Atikah Nur Shabrina Razan Dyan Aprillia Susanti Eka Kumalasari Fradina, IntanTrixi Gatot Ciptadi Gatra Ervi Jayanti Gatra Ervi Jayanti Hakimah, Annisatul Anis Hasan Zayadi Hatif Khusnin Nida' Hikam, Agus Mohammad Husain Latuconsina Islami, Darajatun Islamya, Nur Afni Auli Ismatud Diniyah Istirochah Pujiwati Januarista, Terra Kamilah, Nahdhiyatun Kharisma, Viol Dhea Kurniasari, Yulia Lestari, Tengku Anggun M. Abdul Qodir Jailani Ma'ruf, Muhammad Maghfiroh, Faldha Laili Malia Anjani Moch Mustakim Moch Mustakim Muchammad Faruq Vikriansyah Muhammad Dailami, Muhammad Musaffak, Taufik Ridwan Nabilah Miftachul Khoiriyah Nafisa Nafisa Nia Novita Nurrohmah Nour Athiroh Abdoes Sjakoer Nur Cahya Kartika Slamet Nur Laily Azizah Nur Mufida Nur Mufida Nurrohmah, Nia Novita Nurul Humaidah Oktavia Rahayu Puspitarini Oktaviana, Nimas Ajeng Pinanti, Honesty Nurizza Pratiwi, Radita Intan Aisyah Radita Intan Aisyah Pratiwi Rahayu Dwi Lestari Ramadhan, Majida Ramizard Rafsanjani Rasyadan Taufiq Probojati Rossa Martha Wulandari Rossa Martha Wulandari Saimul Laili Sama’ Iradat Tito Saputri, Syntia Diyah Hayu Ria Silvia, Putri Azizatul Siti Khoirun Ni’mah Siti Qurrotul Aini Sulis Setiawati Suroyya, Mariyam Sutiman B. Sumitro Sutiman Bambang Sumitro Syaiful Arifin Tengku Anggun Lestari Thiya Fathiyatul Fauziyah Tintrim Rahayu, Tintrim Ula, Erfa Navadiatul Umu Intan Kinasih Vina Fitria Wulandari Waliyyul Ahdi Alhikami Widyananda, Muhammad Hermawan Yunani, Silvi