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All Journal AL KAUNIYAH Journal of Biomedicine and Translational Research Jurnal Sumberdaya Hayati Science and Technology Indonesia Jurnal Biologi Tropis Jurnal Kreativitas PKM Jurnal Ilmiah RESPATI INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Indonesian Journal of Halal Research Jurnal Pembelajaran dan Biologi Nukleus Jurnal EduHealth Jurnal Kesehatan Journal of Pharmaceutical and Sciences. Jurnal Sains dan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Jekmal Malau
PT. Sciencewerke Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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In Silico Analysis of Actin Gene as a Candidate for DNA Non-Halal Detection Base on Real-Time PCR Waluyo, Seagames; Malau, Jekmal; Raekiansyah, Muhareva; Yulian, Edwin; Hardiman, Imam
Indonesian Journal of Halal Research Vol. 3 No. 2 (2021): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v3i2.12123

Abstract

Actin genes are genes that are common in organisms, and their expression is constitutive. These genes are used for gene normalization and internal control of DNA extraction, but the actin gene is not widely used for halal certification tests. Bioinformatic studies help to analyze the experiment through in silico more deeply before the experiment is carried out in laboratory, making it more efficient and time effective. uMelt is an analysis to predict the melting curve of target amplification in real-time PCR. Real-time PCR has been widely used for screening and detection of pork content in a product. This research aimed to explore actin gene as a candidate for testing pork using qPCR. The study was carried out in two main stages, namely alignment of the DNA sequence and analysis of the melting curve using the uMelt approach. The results showed a set of actin genes containing conserved regions that can be used as degenerate primers with different family-type coverages. Melting curve prediction with uMelt shows differences in tm peaks so as the types of samples can be easily identified. The use of bioinformatic applications such as uMelt helps in the simulation of predicting the melting curve to increase the precision of the analysis.
Menelusuri Perkembangan, Kondisi Terkini, dan Prospek Masa Depan Pengujian DNA dalam Aplikasi Forensik Manusia dan Non-Manusia: Tinjauan Naratif Siboro, Dewi Pratiwi Purba; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Malau, Jekmal; Aprillia, Cantika; Ainaputri, Aliza Salsabila; Nugraha, Afif Tri; Aprianti, Endeh
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1389

Abstract

DNA typing has become a cornerstone of modern forensic science, profoundly influencing criminal investigations, forensic human identification, and non-human forensic applications. Since its introduction in the mid-1980s, forensic DNA analysis has evolved from restriction fragment length polymorphism-based methods to polymerase chain reaction-based short tandem repeat profiling, and more recently to sequence-based approaches enabled by massively parallel sequencing, resulting in substantial improvements in analytical sensitivity, robustness, and discriminatory power. This narrative review aims to trace the historical development, examine the current state, and explore future directions of DNA typing in both human and non-human forensic contexts, with particular emphasis on empirical case studies from Asia. A narrative review methodology was employed through a comprehensive analysis of peer-reviewed literature published between 2015 and 2026, sourced from major scientific databases including Google Scholar, PubMed, and ScienceDirect, with studies selected based on forensic relevance, methodological rigor, and regional significance. The review highlights the extensive application of DNA typing in routine criminal casework, disaster victim identification, missing persons investigations, wildlife forensic genetics, food fraud detection, and biosecurity, and documents emerging technologies such as portable DNA systems and CRISPR-based detection. Despite these advances, significant challenges remain related to data interpretation, validation requirements, contamination control, ethical and legal governance, and uneven forensic capacity across regions. Overall, this review underscores the continuing evolution of forensic DNA typing and emphasizes the importance of standardized protocols, interdisciplinary collaboration, and region-specific validation to ensure the reliable and responsible application of DNA evidence in modern forensic science.
Integrasi Strategi Genomik Presisi dan Perspektif Etika Islam dalam Pencegahan Stunting: Fokus pada Jalur Hormon Pertumbuhan–IGF-1 Qur’ani , Hifdzil; Nisa, Nasywa Khoirun; Pratama, Pramudya; Kasasiah , Ahsanal; Malau, Jekmal
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 2 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i2.1320

Abstract

Stunting remains one of the major chronic nutritional problems in Indonesia. The national prevalence of stunting has decreased from 37.2% in 2013 to 19.8% in 2024; however, this figure is still above the World Health Organization threshold (<20%) and has not yet reached the national target of 14%. The interaction of nutritional, environmental, and genetic factors influences stunting. Genetic variations within the Growth Hormone–Insulin-like Growth Factor One pathway play a significant role in regulating linear growth in children; disruptions in this pathway can increase vulnerability to stunting. In addition, the majority of Indonesia's population adheres to Islam, making religious values a strategic potential for strengthening health interventions. This review article examines the integration of scientific approaches, focusing on genetics and Islamic values, in stunting prevention by highlighting polymorphisms in the IGF1, IGF1R, and GHR genes and their relevance to the principles of maqāsid al-sharī‘ah, particularly hifz al-nasl (protection of lineage). The analysis is conducted using a descriptive-analytical approach based on recent scientific literature and Islamic references, including the Qur’an, prophetic traditions, and scholarly exegesis. The findings indicate a strong alignment between scientific approaches and Islamic teachings, including the importance of adequate nutrition during pregnancy, exclusive breastfeeding, consumption of lawful, nutritious food, genetic screening efforts consistent with the values of divine decree, and vitamin D supplementation to support Insulin-like Growth Factor One levels. This integration has the potential to enhance community acceptance of stunting-prevention programs, strengthen moral and spiritual motivation, and support the achievement of national stunting-reduction targets. Thus, an approach that combines precise genomics and Islamic values may serve as a contextual and sustainable strategy in shaping a generation that is healthy, intelligent, and spiritually resilient.
Co-Authors Abbas, Zuyyinna Alya Adrian Hartanto, Adrian Ahsanal Kasasiah Ainaputri, Aliza Salsabila Annajla, Fathina Aprianti, Endeh Aprillia, Cantika Astika, Eriyanti Atwita, Syelziva Yonda Damara, Dandy Satria Daniel Joko Wahyono Debora, Priscinya Christiana Dewijanti, Indah D. Edwin Yulian Elyyana, Nina Fikri, Al Mukhlas Hardiman, Imam Hasna, Vina Luthfiana Hermosaningtyas, Anastasia Aliesa Hitopik, Asman Imam Hardiman Imam Hardiman Indah Laily Hilmi Indratno, Saarah Hamidah Asmara Irwansyah, Silvana Lestari Kasasiah , Ahsanal Lestari, Agatha Nabilla Lestari, Sepiyani Ayu Manalu, Rosario Trijuliamos Meilani, Nanda Diva Mierza, Vriezka Muhareva Raekiansyah Muhareva Raekiansyah Munir Alinu Mulki Nisa, Nasywa Khoirun Nugraha, Afif Tri Nur Darmawan, Shiyami Aulia Permatasari, Vera Prastya, Muhammad Eka Pratama, Pramudya Primahana, Gian Purbasari, Dwi Qur’ani , Hifdzil Rahma, Anisa Aula Rahmasari, Ratika Ratnasari, Devi Sa'diyyah, Nur Saryono Seagames Waluyo Seagames Waluyo, Seagames Septi, Annisa Frastica Septiani, Dia Sholih, Mally Ghinan Siboro, Dewi Pratiwi Purba Siti Rohmah Sofia, Siti Sukma Wibowo, Sepvia Putri Sulistiawati Urbaningrum , Lestari Mahardika Urbaningrum, Lestari Mahardika Yulian, Edwin Yuswan, Apriza Zahra, Aliya Azkia Zahro, Aurora Fatimatuz Zakiyah, Wildani