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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA Science and Technology Indonesia Journal of Health (JoH) Indonesian Journal of Chemistry Indonesian Aquaculture Journal Jurnal Biomedika Sains dan Matematika Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Warta LPM Berkala Ilmiah Biologi Indonesian Journal of Biomedicine and Clinical Sciences Jurnal Ilmiah Ilmu Kesehatan
Rarastoeti Pratiwi
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Electric Field-Based Cancer Therapy Induces the Expression of HMGB1 and PD-L1 mRNA Genes on Breast Tumor of Female Rats Fathurrohmah, Siti; Cahyadi, G.A.B Yehezkiel P.; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Cancer Chemoprevention Vol 13, No 2 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss2pp128-136

Abstract

Electro Capacitive Cancer Therapy (ECCT) is an electric field-based cancer therapy method using intermediate frequency (150 kHz) and low intensity (18 Vpp). High Mobility Group Box 1 (HMGB1) is a cytokine related to damage-associated molecular patterns (DAMPs) secreted by dead cells. The expression of Programmed Death Ligand 1 (PD-L1) is ligand present on the surface of tumor cells and its expression is associated with the increase in the number CD8+ T lymphocytes. This study aims to examine the effect of ECCT exposure on the expression of HMGB1 and PD-L1 genes on the breast tumor, brain, and liver tissues of Rattus norvegicus (Berkenhout, 1769). The tissues were obtained from the previous studies stored in RNAlater (-20˚C). Female rat tissues of the previous study from four treatment groups, namely the control group (NINT), non-DMBA-induction with therapy (NIT), DMBA-induction with non-therapy (INT), and DMBA-induction with therapy (IT). Gene expression was analyzed using the RT-qPCR. Statistical t-test with a p<0.05 significance level was performed using GraphPad Prism 9.4.0 software. The result shows HMGB1 and PD-L1 mRNA genes were both significantly expressed in breast tumor samples. The liver and brain samples of normal rats did not show any significant changes in the activity of these genes after exposure to the electric field. This study indicates that exposure to electric fields may trigger the expression of HMGB1 and PD-L on the rat’s breast tumor samples. This study also provides information related to the safety of ECCT in healthy organs of female rats, especially the brain and liver.Keywords: ECCT, breast tumor, HMGB1, PD-L1, IFN- γ.
Pembelajaran Biologi Molekular dan Bioteknologi dalam Konsep Merdeka Belajar Pratiwi, Rarastoeti
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2022: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pendidikan merupakan kebutuhan dasar umat manusia. Setiap insan yang dilahirkan memiliki hak yang sama untuk mendapatkan pendidikan yang layak. Proses pendidikan mencakup 4 pilar pembelajaran, yakni learning to know, learning to do, learning to be, dan learning to live together. Keempat pilar tersebut memiliki aspek yang sangat luas untuk dapat diterapkan. Salah satunya, dikaitkan dalam pembelajaran IPTEK (Ilmu Pengetahuan dan Teknologi) bidang Biologi, khususnya mata pelajaran Biologi Molekuler dan Bioteknologi. Selain itu, pembelajaran dapat dikaitkan dengan tingkatan (leveling) peserta didik, dari tingkat Pra Sekolah (Pendidikan Anak Usia Dini dan Taman Kanak-kanak), Sekolah Dasar, Sekolah Menengah Pertama atau sederajad, Sekolah Menengah atas atau sederajad, hingga Perguruan Tinggi. Pendidikan dan pembelajaran perlu menyesuaikan perkembangan jaman, dengan tantangan masing-masing jaman yang berbeda, khususnya terkait IPTEK. Dalam paparan topik ini, akan diulas lebih jauh tentang pembelajaran Biologi Molekuler dan Bioteknologi dalam konsep Merdeka Belajar, yang dikaitkan dengan tingkatan peserta didik. Dalam rangka mengikuti, mengisi dan mengantisipasi perkembangan jaman terkini hingga berikutnya, Kementerian Pendidikan dan Kebudayaan Republik Indonesia telah menerapkan program Merdeka Belajar. Beberapa konsep tentang program Merdeka Belajar, antara lain arah kebijakan yang meliputi pengembangan literasi, numerasi dan karakter siswa. Selain itu, pelaksanaan proses pendidikan menjadi lebih otonom dengan kultur pembelajaran yang lebih inovatif. Diharapkan dengan konsep Merdeka Belajar, guru dan peserta didik lebih termotivasi dalam mengikuti proses pembelajaran, hingga tercapai tujuan pendidikan maupun pembelajaran secara optimum, serta dilaksanakan secara lebih membahagiakan, termasuk didalamnya pembelajaran untuk mata pelajaran Biologi Molekuler dan Bioteknolgi.
PENGARUH PAKAN EKSOGEN AWAL TERHADAP AKTIVITAS ENZIM PENCERNAAN DAN PERTUMBUHAN LARVA Epinephelus fuscoguttatus (Forsskal, 1775) melianawati, Regina; Pratiwi, Rarastoeti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 14 No. 1 (2022): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v14i1.39801

Abstract

Pemberian pakan eksogen awal merupakan hal yang penting dilakukan dalam kegiatan pembenihan ikan laut, termasuk ikan kerapu macan (Epinephelus fuscoguttatus). Peralihan dari pakan endogen ke pakan eksogen merupakan fase kritis bagi kelangsungan hidup larva ikan kerapu macan pada stadia awal. Keberadaan pakan eksogen dapat berpengaruh terhadap aktivitas enzim pencernaan dan pertumbuhan larva. Penelitian ini bertujuan untuk mengevaluasi peranan pakan eksogen awal terhadap aktivitas enzim pencernaan dan pertumbuhan larva stadia awal. Dua perlakuan yang diujicobakan adalah larva diberi pakan eksogen dan larva tidak diberi pakan eksogen. Pakan eksogen awal yang diberikan berupa zooplankton rotifer Brachionus rotundiformis. Parameter yang diamati adalah aktivitas enzim pencernaan yang meliputi protease, amilase dan lipase; penyerapan pakan endogen; dan pertumbuhan larva yang meliputi panjang total dan bobot tubuh. Hasil penelitian menunjukkan bahwa aktivitas enzim pencernaan larva umur 3 hari yang tidak diberi pakan awal adalah lebih tinggi dibandingkan dengan larva yang diberi pakan eksogen awal. Pakan endogen habis terserap pada larva umur 3 hari. Panjang total larva pada kedua perlakuan adalah cenderung sama, sedangkan bobot tubuh pada larva yang diberi pakan awal lebih besar dibandingkan yang tidak diberi pakan. Hasil penelitian ini menunjukkan bahwa pemberian pakan eksogen awal berpengaruh terhadap aktivitas enzim pencernaan dan pertumbuhan larva ikan kerapu macan pada stadia awal.
A Novel Multiepitope Vaccine for Jembrana Disease: Immunoinformatics, Structural Analysis, Molecular Docking, and Molecular Dynamics Fatimah, Fatimah; Wibowo, Syahputra; Sari, Dini Wahyu Kartika; Pratiwi, Rarastoeti; Kusumawati, Asmarani
Indonesian Journal of Chemistry Vol 26, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.110759

Abstract

Jembrana disease, caused by the Jembrana virus, leads to high mortality (30%) and abortion (49%) in cattle, making vaccination essential. In this study, a multiepitope vaccine was designed using immunogenic TM and CA proteins. Predicted cytotoxic T lymphocyte, helper T lymphocyte, and linear B-cell epitopes were linked with flexible linkers, and the 50S L7/L12 ribosomal protein was added as a TLR4 agonist. In silico analysis confirmed the construct as non-allergenic, antigenic, and thermostable (aliphatic index 85.44), with 94.2% of residues in favored Ramachandran regions. Docking analysis revealed strong binding to TLR4 (−66 kcal/mol), and molecular dynamics simulations validated the structural stability. Immune simulations revealed increased antigen and antibody levels (IgM early, IgG1/IgG2 after day 15), progressive CD4+ T-helper expansion, transient CD8+ T-cell peaks, elevated IFN-γ and IL-2, and strong dendritic cell activation through MHC I and II pathways. These findings indicate the vaccine effectively stimulates humoral and cellular responses, supporting its potential as a promising candidate against the Jembrana virus.
Non-contact electro capacitive cancer therapy (ECCT) modulate the mRNA expression of p53, Apaf-1, survivin, NF-κB, TSP-1 and bFGF in DMBA-induced breast cancer rat Hidayah, Nurul; Putra, Agung; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Biomedicine and Clinical Sciences Vol 58 No 1 (2026)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v58i1.24954

Abstract

Breast cancer is the most common cancer that causes death in women in the world. Cancer development is facilitated by the inhibition of apoptosis and induction of angiogenesis. Current cancer therapy still encounters problems in the form of recurrence, resistance, and side effects of drugs. Non-contact static electric field therapy, electro capacity cancer therapy (ECCT) with medium frequency, is a therapy developed to inhibit the proliferation of tumor cells. This study aimed to determine the mRNA expression of p53, Apaf-1, survivin related to apoptosis and NF-κB, bFGF and TSP-1 related to angiogenesis in rat breast tumor tissue after ECCT frequency of 150 kHz. Breast tissue samples and rat breast tumor nodules stored in RNA later at -20°C were used. The tissue was obtained from the non-induction non-therapy (NINT) group, induction non-therapy (INT), non-induction therapy (NIT), and induction therapy (IT). mRNA expression of p53, Apaf-1, NF-κB, bFGF and TSP-1 were analyzed using qRT-PCR and calculated with the Livak formula. Data were analyzed using one-way Anova and post-hoc LSD. The results showed that, mRNA expression of p53, Apaf-1 and TSP-1 in the IT group increased significantly, and mRNA expression of survivin and bFGF decreased significantly compared to the INT group. However, the expression of NF-κB mRNA in the IT group remained the same as in the INT group. In conclusion, ECCT with a frequency of 150 kHz upregulates p53, Apaf-1 and TSP-1 mRNA expression and downregulates survivin and bFGF mRNA expression but have no effect on NF-κB mRNA expression in rat breast tumor tissue.
Anticancer Efficacy of a Combination of Curcuma longa L. Rhizome and Annona muricata L. Leaf Extracts Against T47D Cells Pebriani, Sonia Ardilla; Bhagawan, Weka Sidha; Prastiyanto, Muhammad Evy; Rukmana, Rizal Maarif; Pratiwi, Rarastoeti; Suhendi, Andi
Science and Technology Indonesia Vol. 11 No. 2 (2026): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.2.405-419

Abstract

Breast cancer remains one of the most prevalent malignancies worldwide. The use of combinations of natural products is increasingly recognized as a promising strategy for cancer treatment. Turmeric rhizome (Curcuma longa L.) and soursop leaves (Annona muricata L.) are two natural materials known for their anticancer potential. This study aimed to identify the phytochemical constituents of turmeric rhizome and soursop leaf extracts and to evaluate the anticancer activity of their combination against T47D breast cancer cells. Turmeric and soursop leaves were extracted with 96% ethanol using the maceration method. Raw material standardization was performed by measuring water content, ethanol-soluble content, and water-soluble content. The extract was standardized by thin-layer chromatography. T47D and Vero cell lines were used in this study. Compound identification was performed using Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry (LC/Q-TOF-MS). The candidate combination ratio was determined using the simplex lattice design approach in Design Expert 13 software. Cytotoxicity and antiproliferative effects were assessed using the MTT assay, while antimetastatic potential was evaluated through the scratch assay. Apoptosis and cell cycle arrest were analyzed by flow cytometry. IC_50 data were analyzed using one-way ANOVA, and post hoc testing was performed using Tukey’s multiple-comparison test. Antiproliferation and scratch assay data were analyzed using two-way ANOVA followed by the Bonferroni test. Apoptosis and cell cycle assay data were analyzed using one-way ANOVA followed by the Tukey post hoc test. Phytochemical profiling indicated the existence of fifteen chemicals in both turmeric rhizome and soursop leaf extracts. The candidate combination ratio of turmeric rhizome to soursop leaf extracts was 1:21, exhibiting cytotoxic activity with an IC50 value of 32.2 ± 2.5 ug/mL. The combined extract was associated with antiproliferative, anti-migratory, and pro-apoptotic responses and induced G2/M phase cell cycle arrest in T47D cells.
Formulasi Natural Antibacterial Facial Wash Berbahan Aktif Infusa Bunga Telang (Clitoria ternatea L.) Yudhatama, Abisha Yoofi; Pratiwi, Rarastoeti; Ningrum, Mutia Cahya; Darmasiwi, Sari
Berkala Ilmiah Biologi Vol 17 No 1 (2026)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v17i1.22050

Abstract

Clitoria ternatea L., or butterfly pea (telang), is a flowering vine known for its high content of anthocyanin, an antibacterial water-soluble phytochemical. Antibacterial properties hold a crucial role as an active compound of personal care product formulations such as facial wash. This research aims to evaluate the antibacterial activity of a facial wash formulation incorporating butterfly pea flower infusion as the active ingredient against S. aureus and P. aeruginosa, known opportunistic pathogens in the skin microbiome. Research methodology includes butterfly pea flower infusion extraction, antibacterial facial wash formulation, agar well diffusion assay, minimum inhibitory concentration (MIC) test, physicochemical analysis, and organoleptic evaluation. Infuse extraction resulted in 10% butterfly pea infusion. Facial wash formulated using a water-based formulation. Agar well diffusion assay showed medium antibacterial strength. Antibacterial results by MIC revealed that the 4% facial wash formulation has the highest antibacterial activity. Physicochemical evaluation showed an average pH value of 6.81, a spread ability of 8.8 cm and 9.1 cm, a foam volume of 123.3 mL, and a foam height of 11.2 cm. Organoleptic assessment indicated that panelists rated the antibacterial facial wash formulation as satisfactory to good. In conclusion, the butterfly pea flower antibacterial facial wash formulation is effective against S. aureus and P. aeruginosa and is well-accepted by potential consumers.
Co-Authors A.A. Ketut Agung Cahyawan W Adania, Baik Aisyah Afifah, Fitri Nur Agung Putra ALIM ISNANSETYO Almunawar, Muhammad Fikri Andi Suhendi Ardaning Nuriliani Asmarani Kusumawati Asmarani Kusumawati Cahyadi, G.A.B Yehezkiel P. Christina Retna Handayani Christina Retna Handayani, Christina Retna Darmasiwi, Sari Dewi Nur&#039;aeni Setyowati Endah Sri Palupi Fahhlevi, Nabilah Adzra Fajar Sofyantoro Fathurrohmah, Siti fatimah Fatimah Feri Sukmawati Firman Alamsyah Hartati, Yuni Hidayat, Rheina Faticha Asyamsa L. Hartanto Nugroho Lanjar Lisna Hidayati, Lisna Maharesi, Chesa Ekani Masashi Kawaichi Masashi Kawaichi, Masashi Mataram, Made Bagus Auriva Maturbongs, Agatha Cecilia Maturbongs, Rudi A. Maulina, Nurina Tahta Afwi Meti Indrowati Mulyati-Sarto Mulyati-Sarto Mulyati-Sarto, Mulyati-Sarto Murwantoko . Nasution, Nurlita Putri Bela Ni Wayan Widya Astuti Niken Satuti Nur Handayani Ningrum, Mutia Cahya Nurhidayah, Septika Nurul Hidayah Nurul Inayah nuryastuti, Triana Nyoman Puniawati Soesilo Palupi, Endah Sri Paramita Sari, Indah Pebriani, Sonia Ardilla Penny Humaidah Hamid Penny Humaidah Hamid, Penny Humaidah Prastiyanto, Muhammad Evy PUDJI ASTUTI Purnami, S.E. raudatul jannah Regina Melianawati Rina Puji Astuti Rizal Maarif Rukmana Rizal Maarif Rukmana, Rizal Maarif Rumiyati, Rumiyati Sarbini Sari, Dini Wahyu Kartika Soesilo, Nyoman Puniawati Soesilo, Nyoman Puniawati SUWARNO HADISUSANTO Syam, Adi Mazdi Trijoko Trijoko Trijoko Trijoko Weka Sidha Bhagawan Wibowo, Syahputra Yekti Asih Purwestri Yudhatama, Abisha Yoofi