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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Prosiding Seminar Biologi Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Cancer Chemoprevention Prosiding Pendidikan Sains Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA Journal of Health (JoH) Indonesian Aquaculture Journal Jurnal Biomedika Sains dan Matematika Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Warta LPM Jurnal Ilmiah Ilmu Kesehatan
Rarastoeti Pratiwi
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Therapeutic Effects of BRC Functional Food from Indonesian Black Rice on Body Weight and Haematological Parameters in Obese Rats Sofyantoro, Fajar; Syam, Adi Mazdi; Adania, Baik Aisyah; Almunawar, Muhammad Fikri; Nasution, Nurlita Putri Bela; Hidayat, Rheina Faticha Asyamsa; Mataram, Made Bagus Auriva; Maharesi, Chesa Ekani; Nurhidayah, Septika; Purwestri, Yekti Asih; Nuriliani, Ardaning; Hidayati, Lisna; Pratiwi, Rarastoeti
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.85847

Abstract

Obesity increases the risk of various diseases. Black rice, renowned for its high anthocyanin content, is considered a potential functional food for preventing metabolic disorders. The current study investigated the effects of black rice crunch (BRC) on body weight and haematological profiles in obese rats. Rats were fed with high-fat diet to induce obesity and supplemented with different concentrations of BRC for 4 and 8 weeks. The results showed that high-fat diet effectively induced obesity, as evidenced by significant increase in body weight. Importantly, 75% BRC supplementation resulted in significant weight reduction in obese rats. Further analysis revealed an increase in erythrocyte numbers in obese groups supplemented with 75% BRC, but no significant changes in haemoglobin concentration or haematocrit percentage. Further investigation showed that 75% BRC led to a decrease in mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and mean corpuscular volume (MCV), potentially affecting the size and concentration of haemoglobin within erythrocytes. The total leucocytes count increased with the high-fat diet, while BRC supplementation alone did not have significant impact. Lymphocyte percentage remained stable across the groups, indicating minimal influence of the dietary interventions. Neutrophil percentage varied initially but was not specific to BRC or the high-fat diet. Platelet count and distribution width were not significantly influenced, but mean platelet volume (MPV) increased after 8 weeks of BRC treatment, suggesting larger platelet sizes associated with obesity. Overall, the study provides important insights into the effects of BRC supplementation on body weight and haematological parameters related to obesity. 
Chitosan Potential as Delivery Agent for S1 Gene from SARS-CoV-2 with pEGFP-N1 as its Vectors: Potensi Kitosan sebagai Sistem Penghantaran Gen S1 Virus SARS-CoV-2 dengan Vektor pEGFP-N1 Kusumawati, Asmarani; Fahhlevi, Nabilah Adzra; Pratiwi, Rarastoeti; Jannah, Raudatul; Maulina, Nurina Tahta Afwi
Journal of Health (JoH) Vol 11 No 02 (2024): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v11n2.716

Abstract

Coronavirus Disease-19 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), this disease began to emerge at the end of 2019 and has become a pandemic to this day. Vaccination can play an important role to prevent the spread of the disease, so there are many vaccine developments, including DNA vaccines. Delivery systems such as chitosan can increase the effectiveness of vaccines. The purpose of this study was to optimize and evaluate the pEGFP-N1-S1 formula with a chitosan delivery system as a DNA vaccine candidate. Plasmids were cloned in E.coli DH5α and extracted for further evaluation with restriction enzymes and sequencing. The EGFP-N1-S1 plasmid was then formulated with chitosan nanoparticles with a mass ratio of Plasmid DNA:chitosan of 1:0.1; 1:0.2; 1:0.3; 1:0.4; 1:0.5; 1:0.6 and 1:0.7. The isolated plasmid has a concentration of 2182.32 ng/L and a purity of 1.911. Restriction results showed that the S1 gene (684 bp) was successfully inserted into the pEGFP-N1 plasmid (4813 bp). DNA:chitosan mass ratio of 1:0.6 is the optimal formulation for DNA to bind with chitosan perfectly.
POTENSI EKSTRAK ORGAN VEGETATIF ANGGREK VANDA HASIL PERSILANGAN SEBAGAI AGEN ANTIKANKER Maturbongs, Agatha; Pratiwi, Rarastoeti; Nugroho, L. Hartanto; Maturbongs, Rudi A.
Jurnal Ilmiah Ilmu Kesehatan Vol. 1 No. 2 (2023): Volume 1 Nomor 2 Tahun 2023
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jiik.v1i2.12908

Abstract

Penelitian ini bertujuan untuk menguji sitotoksisitas fraksi ekstrak kloroform gabungan organ vegetatif Vanda hasil persilangan terhadap kanker payudara cell line T47D dan kanker serviks cell line HeLa. Organ vegetatif dari tanaman tersebut diekstrak dan difraksinasi. Hasil fraksinasi dengan profil yang sama digabungkan. Fraksi gabungan diuji sitotoksisitasnya terhadap cell line T47D dan cell line HeLa menggunakan metode MTT Assay. Analisis data untuk penentuan IC50 dihitung dengan analisis probit dalam SPSS 17. Hasil penelitian menunjukan fraksi C yang paling baik dalam menghambat pertumbuhan sel kanker payudara cell line T47D dan sel kanker serviks cell line HeLa.
Electric Field-Based Cancer Therapy Induces the Expression of HMGB1 and PD-L1 mRNA Genes on Breast Tumor of Female Rats Fathurrohmah, Siti; Cahyadi, G.A.B Yehezkiel P.; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Cancer Chemoprevention Vol 13, No 2 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss2pp128-136

Abstract

Electro Capacitive Cancer Therapy (ECCT) is an electric field-based cancer therapy method using intermediate frequency (150 kHz) and low intensity (18 Vpp). High Mobility Group Box 1 (HMGB1) is a cytokine related to damage-associated molecular patterns (DAMPs) secreted by dead cells. The expression of Programmed Death Ligand 1 (PD-L1) is ligand present on the surface of tumor cells and its expression is associated with the increase in the number CD8+ T lymphocytes. This study aims to examine the effect of ECCT exposure on the expression of HMGB1 and PD-L1 genes on the breast tumor, brain, and liver tissues of Rattus norvegicus (Berkenhout, 1769). The tissues were obtained from the previous studies stored in RNAlater (-20˚C). Female rat tissues of the previous study from four treatment groups, namely the control group (NINT), non-DMBA-induction with therapy (NIT), DMBA-induction with non-therapy (INT), and DMBA-induction with therapy (IT). Gene expression was analyzed using the RT-qPCR. Statistical t-test with a p<0.05 significance level was performed using GraphPad Prism 9.4.0 software. The result shows HMGB1 and PD-L1 mRNA genes were both significantly expressed in breast tumor samples. The liver and brain samples of normal rats did not show any significant changes in the activity of these genes after exposure to the electric field. This study indicates that exposure to electric fields may trigger the expression of HMGB1 and PD-L on the rat’s breast tumor samples. This study also provides information related to the safety of ECCT in healthy organs of female rats, especially the brain and liver.Keywords: ECCT, breast tumor, HMGB1, PD-L1, IFN- γ.
Pembelajaran Biologi Molekular dan Bioteknologi dalam Konsep Merdeka Belajar Pratiwi, Rarastoeti
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2022: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pendidikan merupakan kebutuhan dasar umat manusia. Setiap insan yang dilahirkan memiliki hak yang sama untuk mendapatkan pendidikan yang layak. Proses pendidikan mencakup 4 pilar pembelajaran, yakni learning to know, learning to do, learning to be, dan learning to live together. Keempat pilar tersebut memiliki aspek yang sangat luas untuk dapat diterapkan. Salah satunya, dikaitkan dalam pembelajaran IPTEK (Ilmu Pengetahuan dan Teknologi) bidang Biologi, khususnya mata pelajaran Biologi Molekuler dan Bioteknologi. Selain itu, pembelajaran dapat dikaitkan dengan tingkatan (leveling) peserta didik, dari tingkat Pra Sekolah (Pendidikan Anak Usia Dini dan Taman Kanak-kanak), Sekolah Dasar, Sekolah Menengah Pertama atau sederajad, Sekolah Menengah atas atau sederajad, hingga Perguruan Tinggi. Pendidikan dan pembelajaran perlu menyesuaikan perkembangan jaman, dengan tantangan masing-masing jaman yang berbeda, khususnya terkait IPTEK. Dalam paparan topik ini, akan diulas lebih jauh tentang pembelajaran Biologi Molekuler dan Bioteknologi dalam konsep Merdeka Belajar, yang dikaitkan dengan tingkatan peserta didik. Dalam rangka mengikuti, mengisi dan mengantisipasi perkembangan jaman terkini hingga berikutnya, Kementerian Pendidikan dan Kebudayaan Republik Indonesia telah menerapkan program Merdeka Belajar. Beberapa konsep tentang program Merdeka Belajar, antara lain arah kebijakan yang meliputi pengembangan literasi, numerasi dan karakter siswa. Selain itu, pelaksanaan proses pendidikan menjadi lebih otonom dengan kultur pembelajaran yang lebih inovatif. Diharapkan dengan konsep Merdeka Belajar, guru dan peserta didik lebih termotivasi dalam mengikuti proses pembelajaran, hingga tercapai tujuan pendidikan maupun pembelajaran secara optimum, serta dilaksanakan secara lebih membahagiakan, termasuk didalamnya pembelajaran untuk mata pelajaran Biologi Molekuler dan Bioteknolgi.
PENGARUH PAKAN EKSOGEN AWAL TERHADAP AKTIVITAS ENZIM PENCERNAAN DAN PERTUMBUHAN LARVA Epinephelus fuscoguttatus (Forsskal, 1775) melianawati, Regina; Pratiwi, Rarastoeti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 14 No. 1 (2022): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v14i1.39801

Abstract

Pemberian pakan eksogen awal merupakan hal yang penting dilakukan dalam kegiatan pembenihan ikan laut, termasuk ikan kerapu macan (Epinephelus fuscoguttatus). Peralihan dari pakan endogen ke pakan eksogen merupakan fase kritis bagi kelangsungan hidup larva ikan kerapu macan pada stadia awal. Keberadaan pakan eksogen dapat berpengaruh terhadap aktivitas enzim pencernaan dan pertumbuhan larva. Penelitian ini bertujuan untuk mengevaluasi peranan pakan eksogen awal terhadap aktivitas enzim pencernaan dan pertumbuhan larva stadia awal. Dua perlakuan yang diujicobakan adalah larva diberi pakan eksogen dan larva tidak diberi pakan eksogen. Pakan eksogen awal yang diberikan berupa zooplankton rotifer Brachionus rotundiformis. Parameter yang diamati adalah aktivitas enzim pencernaan yang meliputi protease, amilase dan lipase; penyerapan pakan endogen; dan pertumbuhan larva yang meliputi panjang total dan bobot tubuh. Hasil penelitian menunjukkan bahwa aktivitas enzim pencernaan larva umur 3 hari yang tidak diberi pakan awal adalah lebih tinggi dibandingkan dengan larva yang diberi pakan eksogen awal. Pakan endogen habis terserap pada larva umur 3 hari. Panjang total larva pada kedua perlakuan adalah cenderung sama, sedangkan bobot tubuh pada larva yang diberi pakan awal lebih besar dibandingkan yang tidak diberi pakan. Hasil penelitian ini menunjukkan bahwa pemberian pakan eksogen awal berpengaruh terhadap aktivitas enzim pencernaan dan pertumbuhan larva ikan kerapu macan pada stadia awal.
Co-Authors A.A. Ketut Agung Cahyawan W Adania, Baik Aisyah Afifah, Fitri Nur ALIM ISNANSETYO Almunawar, Muhammad Fikri Ardaning Nuriliani Asmarani Kusumawati Asmarani Kusumawati Cahyadi, G.A.B Yehezkiel P. Christina Retna Handayani Christina Retna Handayani, Christina Retna Dewi Nur&#039;aeni Setyowati Endah Sri Palupi Fahhlevi, Nabilah Adzra Fajar Sofyantoro Fathurrohmah, Siti Feri Sukmawati Firman Alamsyah Hartati, Yuni Hidayat, Rheina Faticha Asyamsa L. Hartanto Nugroho Lanjar Lisna Hidayati, Lisna Maharesi, Chesa Ekani Masashi Kawaichi Masashi Kawaichi, Masashi Mataram, Made Bagus Auriva Maturbongs, Agatha Maturbongs, Rudi A. Maulina, Nurina Tahta Afwi Meti Indrowati Mulyati-Sarto Mulyati-Sarto Mulyati-Sarto, Mulyati-Sarto Murwantoko . Nasution, Nurlita Putri Bela Ni Wayan Widya Astuti Niken Satuti Nur Handayani Nurhidayah, Septika Nurul Inayah Nyoman Puniawati Soesilo Palupi, Endah Sri Paramita Sari, Indah Penny Humaidah Hamid Penny Humaidah Hamid, Penny Humaidah PUDJI ASTUTI Purnami, S.E. raudatul jannah Regina Melianawati Rina Puji Astuti Rizal Maarif Rukmana Rumiyati, Rumiyati Sarbini Soesilo, Nyoman Puniawati Soesilo, Nyoman Puniawati SUWARNO HADISUSANTO Syam, Adi Mazdi Triana Nuryastuti Trijoko Trijoko Trijoko Trijoko Yekti Asih Purwestri