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All Journal KAPAL Jurnal Ilmu Pengetahuan dan Teknologi Kelautan Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik agriTECH Jurnal Sumberdaya Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology AL-HAYAT: Journal of Biology and Applied Biology
Sri Pujiyanto
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Other

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Kandungan Proksimat dan Daya Pengikatan Tepung Iles-Iles (Amorphophallus oncophylus) Terhadap Aflatoksin Sebagai Upaya Pencarian Bahan Pengikat Alternatif pada Pakan Sigit Hananto; Sri Pujiyanto; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aflatoxin  is  a  metabolite  secondary  of  Aspergillus  flavus  and  A.  Parasiticus. Contamination  aflatoxin in poultry have an impact on their liver damage. Technique to reduce the effects of aflatoksin can be done by addition of aflatoxin binder. Aflatoxin binder material commonly used still import as  glucomannan yeast product (GYP), so we needed study local material that has the potential  as a a  aflatoxin binder. The purposes is  test proximate content and the binding power of flour iles-iles compared  to GYP. Sample in research  is  flour  derived  from  bulbs  iles-iles  (Amorphophalus  oncophylus)  and  GYP  as control. The parameters in research that is the physical characteristics , proximate analysis , glukomannan levels and the ability of binding aflatoksin in vitro between flour iles-iles and GYP. The results of the physical test, the sample has delicate texture and gross energy flour iles-iles not significantly different with GYP. The results of proximate analysis ( the water level , ashes , crude fiber , crude protein and crude fat) show between flour iles-iles and GYP was significantly different, except crude fat that shown the result is no different (p<0,05) .The results of the analysis in the glukomannan levels of flour iles-iles is 52,74 % and GYP 17,36%. Testing the ability bind done with a solution of aflatoksin 2 ml (2.7 ng/ml) and materials binder used is 0  g; 0,0013 g; 0,0027 g and 0,0040 g. The research results show binding aflatoxin by flour iles-iles optimal in heavy 0,0013 g (92,92 %), while binding aflatoxin by GYP optimal in heavy 0,0040 g (87,71 %). The ability binding aflatoksin flour iles-iles in proportion to GYP so that can be used as a alternative binder in feed. Keyword: Iles-iles (Amorphophallus oncophylus), aflatoxin, Glucomannan 
Isolasi Bakteri Endofit Bangle (Zingiber cassumunar Roxb.) dan Uji Antibakteri Supernatan Crude Metabolit Sekunder Isolat Potensial terhadap Staphylococcus aureus Maulida Aglinia; Sri Pujiyanto; Wijanarka Wijanarka
Jurnal Akademika Biologi Vol. 9 No. 1 Januari 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Infeksi nosokomial adalah infeksi yang terjadi pada pasien selama menerima perawatan kesehatan dan berkembang dalam 48-72 jam setalah dirawat di RS, menyebabkan morbiditas dan mortalitas yang signifikan pada pasien. Mikroorganisme yang sering menyebabkan infeksi nosokomial adalah Staphylococcus aureus. Bangle (Zingiber cassumunar Roxb.) merupakan salah satu tanaman obat tradisional Indonesia yang digunakan masyarakat untuk obat diare dan larutan pembersih karena infeksi. Tujuan penelitian ini yaitu untuk mengetahui aktivitas antibakteri dari supernatan crude metabolit sekunder bakteri endofit Bangle dalam menghambat pertumbuhan Staphylococcus aureus. Isolasi bakteri endofit dari bagian daun, batang, rimpang dan akar tanaman agar diperoleh isolat murni untuk diseleksi aktivitas antibakterinya dan didapatkan isolat potensial. Hasil isolasi diperoleh 16 isolat, 3 isolat diantaranya berpotensi memiliki aktivitas antibakteri terhadap bakteri uji dengan kode isolat Ri1, Ri4 dan Ak1. Hasil uji antibakteri supernatan diperoleh rata-rata diameter zona hambat terhadap S. aureus oleh Ri1, Ri4 dan Ak1 berturut-turut sebesar 6,98 mm; 8,12 mm; dan 9,25 mm. Hasil analisis statistik menunjukkan signifikansi nilai F hitung < F tabel 0,05 sehingga pengaruh aktivitas antibakteri supernatan isolat endofit potensial terhadap S. aureus berbeda secara signifikan satu dengan lainnya. Aktivitas antibakteri isolat Ak1 terhadap bakteri uji menunjukkan efek paling baik.
AKTIVITAS INHIBITOR Α-AMILASE ISOLAT KHAMIR ENDOFIT DARI TUMBUHAN BROTOWALI (Tinospora crispa L.) Pramita Dian Pramitasari; Sri Pujiyanto; Agung Suprihadi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus is a carbohydrate, fat, and protein metabolism disorder characterized by high blood sugar level. The α-amylase inhibitor may inhibit the carbohydrates digestion and cause a rate decrease of glucose absorption. The α-amylase inhibitors are found in a plant that potentially containing antidiabetic compounds, such as Brotowali (Tinospora crispa L.). The exploration of endophytic microbial is expected can produce secondary metabolites with similar properties to those produced by host plant metabolites. The purpose of this research were to obtain endophytic yeast isolates which can produce the best α-amylase inhibitors and to determine the ability of selected isolates to produce α-amylase inhibitors on varied carbon source and pH production media. This research was conducted by isolating endophytic yeast from roots, stems, and leaves of brotowali. The best isolate was selected by considering its inhibitory activity. The α-amylase inhibitor test was determined by measuring the resulting reduction sugar. This research used Completely Randomised Design. First treatment was carbon source media, such as starch, sucrose, maltose and lactose. The best carbon source then was used for further tests. Second treatment was pH media including pH of 5, 6, 7 and 8. The data obtained later were analyzed using One Way ANOVA. The selection results showed that  DG26 isolate had the best α-amylase inhibition value of 68.27% with 72 hours optimal time production. The statistical result test on the varied carbon source treatment showed insignificant effect (P> 0,05) with the best α-amylase inhibition value of 19,98% at starch treatment. The statistical result test on the varied pH treatment showed a significant different effect (P <0.05) with the best α-amylase inhibition value of 44.91% at pH 7.Keywords: α-amylase inhibitor, endophytic yeast, Tinospora crispa, carbon source, pH
Produksi Pigmen Merah Monascus sp. pada Substrat Tepung Biji Durian (Durio zibethinus Murr.) dan Biji Nangka (Artocarpus heterophyllus Lam.) dengan Variasi Penambahan Sumber Nitrogen Tsania Dyna Falasifa; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Public awareness about the danger of using synthetic dyes impact on health for a long term, pushes the demand of natural dye that is healthier. One of the natural dye is Monascus sp. red pigment which traditionally uses the rice substrate. Durian seed and jackfruit seed are residue from agroindustry which still have high nutritional value thus potentially become the alternative substrate. The aim of this study was to measured Monascus sp. red pigment production in the flour substrate of durian seed and jackfruit seed with variant nitrogen source added. This research used completely randomized design (CRD) factorial with 2 factors. The first factor was a kind of substrate fermentation (N): jackfruit seed flour and (D): durian seed flour. The second factor was concentration peptone added (P0): 0%, (P1): 1%, (P2): 2%, and (P3): 3%. Research consist of 8 treatments with 3 replications. Research parameters were red pigment concentration and biomass estimation. Analysis of the data used Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at the 95% significance level and Pearson correlation test. The result showed that Monascus sp. can  grew and producted red pigment on both of substrate with the addition of a nitrogen source variation. Fermentation on the jackfruit seed flour substrate with 2% peptone added was the most effective in red pigment production  with 5,55 CVU/gds. The addition of 2% peptone on both types of substrate yielded highest biomass with 1.53 mg/gds, and there was a positive correlation between the biomass and the red pigment production. Keywords: Pigment, Monascus sp., Durio zibethinus, Artocarpus heterophyllus, nitrogen
UJI ANTAGONIS KAPANG ENDOFIT DUWET (Syzigium cumini (L.) Skeels) TEHADAP KAPANG Fusarium oxysporum PENYEBAB PENYAKIT MOLER PADA BAWANG MERAH (Allium ascalonicum L.) SECARA IN-VITRO Luthfian Nur Afifi; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Endophytic fungi inhabit plant hosts for all or part of their life cycle in plant tissues without doing harm to its host. Utilization of endophytic fungi as controlling plant pathogens has been widely studied in in-vivo and in-vitro. The aims of this study was to determined the antagonistic activities of 8 endophytic fungal isolates from some parts of duwet (Syzigium cumini (L.) Skeels.) tree against F. oxysporum, a pathogenic fungi causing bulb rot disease on red onion. This study was conducted using Completely Randomized Design (CRD), with 8 endophytic fungal isolates as treatment, done in triplicates. The antagonistic activity were examined using dual culture method, by determined the percentage of inhibition. The mycelial plugs (4 mm diameter) of endophytic fungi and pathogenic fungi F. oxysporum were placed in same dish 3 cm each other. The endophytic fungal isolates were conventionally identified to genus level. The identification results showed that endophytic fungi came from 1 isolates of Aspergillus, 5 isolates of Penicillium, 1 isolate of Fusarium, and 1 isolate of Mycelia sterilia. All endophytic fungal isolates showed capability on inhabiting the growth of F. oxysporum with the percentage of inhibition varied between 1.1 to 36.0%. Fusarium JD1 isolates showed the highest antagonist activity against F. oxysporum (36.0%). Keywords : Endophytic fungi, duwet, Fusarium oxysporum, growth inhibition.
ISOLASI DAN KARAKTERISASI ISOLAT KONSORSIUM BAKTERI LAHAN PERTANIAN SEBAGAI POTENSI DEGRADASI PESTISIDA PROPOXUR Ahmad Qi Sahlan; Endang Kusdiyantini; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Propoxur pesticide is a pesticide used widely enough by the Indonesian agricultural community. This pesticide is used as a systemic pesticide in agricultural areas to deal with pests as well as insecticides, herbicides, and bactericide. Study of the degradation of propoxur has long performed using the bacterial bioremediation agent. Bacterial consortium is a combination of several bacteria in a medium which that has a complementary function in the environment. The purpose of this study was the isolation and characterization of a consortium of farms that use propoxur pesticide. Isolation was done by spread plate and was observed characteristics of colony morphology. The obtained isolates were tested against propoxur and its potential role in the consortium. Degradation of propoxur observations using High Performance Liquid Cromatography (HPLC). Isolation results obtained 8 (eight) bacterial isolates and one that have an important role in the consortium is K1 isolate. K1 isolate were able to work up a consortium to degrade Propoxur in 2 isolates with K2 isolate. The interaction of these bacteria are natural and have a tendency to vary in nature after a process of isolation. K1 isolate are not able to work alone in a mineral medium, and worked with synergistic and complementary that in the bacterial consortium. KSR consortium optimum growth occurs in an environment with temperature 35 °C and pH 6.Keywords: Isolation, Propoxur, Bioremediation, Bacterial Consortium, HPLC
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN JAGUNG (Zea mays) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Debby Widiyanti; Sri Pujiyanto; Agung Suprihadi; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Maize (Zea mays) is the second most important food commodity after rice plants. The role of the corn crop in agricultural commodities encourage the use of biological fertilizers to increase crop productivity of maize. The use of biological fertilizers open opportunities using rhizosphere bacteria as inoculants boosters grow plants.This study aims to obtain bacterial isolates from the rhizosphere of maize (Zea mays), characterize the morphology and biochemical properties of the ability to produce IAA, provide nutrients such as N and P and to get a bacterial inoculant boosters grow corn. Rhizosphere bacteria were isolated using SEA media (soil extract agar). Isolates that have the ability to produce IAA, tether N and high P solvents tested against corn seed germination and applied in a phase of plant growth. The results showed there were 24 isolates corn crop rhizosphere dominated by gram-positive bacteria and shaped basil capable of producing IAA grading 4,83 to 125,84 ppm. 16 rhizosphere bacterial isolates capable of dissolving phosphate with phosphate solubility index ranged from 2,1 to 4,6. 18 rhizosphere bacterial isolates capable of tether N with the highest N tether capacity of 2,8 x 10 3 CFU/ mL. Application 3 selected bacteria such as J11, J16 and J19 as inoculants are not able to increase the growth of corn plants compared to control water. Keyword :Maize, Rhizosphere, The growth of corn plants.
POTENSI RIZOBAKTERI PEMBENTUK ENDOSPORA DARI TANAMAN PADI SEBAGAI BIOKONTROL FITOPATOGEN Xanthomonas oryzae Maerani Sumarno; Anto Budiharjo; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Xanthomonas oryzae is phytopathogen causing bacterial leaf blight which decreases in agricultural product reaching 20-70 % in Asia. Bacterial leaf blight symptoms is characterized by the formation of lines in the leaf blade turnings yellow, then white, causing the plant to wither and die. Endospore-forming rhizobacteria are soil microbes potential as biocontrol to inhibit phytopathogen growth. The aims of this study were to isolate endospore-forming rhizobacteria from rice plant and determine its ability as biocontrol against X. oryzae. The methods used consisted of isolation, antibacterial activity test, molecular identification, and biochemical characterization. Twenty isolates of endospore-forming rhizobacteria were obtained from the isolation of the rice crop. Isolate P-10 had the greatest ability against X. oryzae with inhibition zone of 18.89 mm. Molecular identification using 16S rRNA gene showed that isolates P-10 had 98 % homology with Bacillus pumilus. Biochemical characterization showed the isolate P-10 had a rod-shaped with center of endospores, gram-positive, catalase positive, are motile, negative in starch hydrolyze, not forming gas on glucose, these characteristics fitted with B. pumilus character. Keywords : Rice plant, antibacterial, Xanthomonas oryzae, endospore-forming, rhizobacteria
Produksi Enzim Protease Dari A.niger PAM18A dengan Variasi pH dan Waktu Inkubasi Putri Ramadhani; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Advances in the field of fermentation technology, genetic engineering, and technology applications of enzymes lead to the use of enzymes in the industry is increasing. Enzymes used in the industry can be derived from microorganisms such as bacteria, molds, and yeasts. Protease is one of the important enzyme that has been used widely in various fields of industrial application and 65% of the sales of enzymes in the world. Alkaline protease is one of hydrolytic enzymes that can hydrolysis proteins which working at the pH range of 7-12. The aim of this study was  to investigate the influence of pH and incubation time on the protease production of A. niger PAM 18A. The experimental were done using Factor Block Random Design with 2 i.e pH of7, 8, 9, and incubation time of 5, 6, 7 days. The results showed that highest protease fromA. niger PAM18A were found in the treatment of pH 8 and of 5 days incubating with the protease activity and specific activity were 0,930 U/mL and 3,632U/mg respectively.Keywords: Production Enzyme, Protease, A. niger PAM18A, pH, incubation time.
ISOLASI, UJI AKTIVITAS, DAN OPTIMASI INHIBITOR α-AMILASE ISOLAT KAPANG ENDOFIT TANAMAN BINAHONG (Anredera cordifolia) (Ten.) Steenis Diani Ajeng Prahesti; Sri Pujiyanto; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus (DM) is a hyperglycemia disease caused by insensitivity of cells to insulin that causes the presence of glucose in blood and urine. The α-amylase inhibitors is a compound that inhibit carbohydrates breakdown into glucose by amylase enzyme. Binahong (Anredera cordifolia) has been used by Indonesian people since long time ago to treat various diseases such as for anti-diabetic. The aims of study were to obtained the endophytic fungal isolated from Binahong plant, and find out of their ability of α-amylase inhibitor activity by treatment of carbon source and pH of production medium. The isolating endophytic fungal from binahong roots, stems, and leaves in medium PDA. The α-amylase inhibitory test was using starch substrate and the α-amylase inhibitory measure using spectrophotometric method. The study was used one factor CRD i.e. carbon source including sucrose, lactose, and maltose. The best carbon source then continued to test with variation pH 5,6, and 7. The data obtained were then analyzed by Analysis of Variance (ANOVA). Five endophytic fungal isolated from binahong were only one isolated i.e DT should high potensial in inhibiting α-amylase 91,43%. The best carbon source for inhibitory α-amylase i.e sucrose with the percentage of 58,91%, while pH 5 is the best pH medium production continous treatment carbon source sucrose with the percentage 37,83%. The result of statistical test showed that sucrose as the best carbon source just than lactose and maltose, and pH 5 as the best medium pH for the production α-amylase inhibitor. Key words : α-amylase inhibitor, endophyte fungi, Anredera cordifolia, diabetes mellitus
Co-Authors Achirul Nditasari Agung Suprihadi Agung Suprihadi Ahmad Qi Sahlan Anang S. Achmadi Anggraeni, Via Annisa Widyasari Anto Budiharjo Aqlina, Maulida Ari Wibawa Budi Santosa Arina Tri Lunggani Ariyani, Mei Dwi Aviany, Hanna Berliana Bambang Sri Waluyo Budi Raharjo Budi Raharjo Bunga Fajriani Chang, Helen Choirunnisaa, Nadia Maharani Jasmine Debby Widiyanti Dewanti, Ardelia Dewi, Tirta Kumala Dewi, Yulita Wiwik Irana Diani Ajeng Prahesti Dinda Khairunnisa, Dinda Dwi Retnowati Elawati, Nunung Eni Elawati, Nunung Eni Endang Kusdiyantini Endang Kusdiyantini Erfianti, Tia Fahrurrozi Fahrurrozi Fathmah, Ema Nuzula Fathmah, Ema Nuzulah Fatin, Nuhaul Fitri Ulfana Risky Fitria Fitria Gabriela Christy Sabbathini Genoveva Preta Angelika Ghaida Afra Akhsani Halwiyah, Nurul Hapzi Ali Hermin Pancasakti Kusumaningrum Hery Widijanto Hessy Novita Jepri Agung Priyanto, Jepri Agung Larasati, Dinar Rahmi Luthfian Nur Afifi M Muslikha Maerani Sumarno Mamik Setyowati Maulida Aglinia Mawarni, Shelina Nurunnisa MG Isworo Rukmi Moi, Maria Yasinta Mulyani, Nies S Nadina, Rahmah Qisti Nafisah, Hidayatun Nandina, Rahmah Qisti Nanik Rahmani NANIK RAHMANI Novi Alvita Pratama Noviasih, Ria Frasida Nunung Eni Elawati Nunung Eni Elawati Nurul Inayah Oktavia, Anik Oktavia, Nurrizqi Permatasari, Vera Pramita Dian Pramitasari Prastya, Muhammad Eka Pratama, Novi Alvita Primahana, Gian Putra, Mohammad Affan Dwica Putri Ramadhani Putri, Adde Lolita Octavia R. Rahardian Rahmah, Chairunnisaa Jabal Rahmandanni, Yunnia Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi RATIH DEWI HASTUTI Rejeki Siti Ferniah Resdiani, Merysa Ridho Mathori Ikhwan Rifka A. N. Safitri Ristia Rachmatunnisa Riza Laksita Devi Mutiaratri Rizko, Nurmalisa Roseliana Fitri Roslenawati Roslenawati RS Ferniah Sari, Suli Arum Sarsa A. Nisa Setiawan, Ruby Sigit Hananto Siti Nur Jannah Siti Nur Jannah Sri Rahayu Tri Astuti Sugiyono Saputra Sumarno, Maerani Sunarno s Sunarno Sunarno Sunarno Sunarno Suprihadi Supriyadi Supriyadi Susiana Purwantisari T. A. Lestari Taufiq P. Nugraha Tri Rahayu, Hesti Tsania Dyna Falasifa Utami, Linda Ayu w wijanarka Wahyu Aji Mahardhika Wandita, Ryan Hilda Wigunarti, Anggia Hesti Wijanarka Wijanarka Wijanarka, W Wijarnaka, Wijarnaka Yopi Yopi YOPI YOPI Yudi Yunanto Yuswan, Apriza