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All Journal KAPAL Jurnal Ilmu Pengetahuan dan Teknologi Kelautan Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik agriTECH Jurnal Sumberdaya Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology AL-HAYAT: Journal of Biology and Applied Biology
Sri Pujiyanto
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Other

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Kemampuan Isolat Fungi Endofit Tanaman Nilam (Pogostemon cablin) sebagai Penghasil Antimikroba terhadap Escherichia coli dan Staphylococcus aureus Novi Alvita Pratama; Endang Kusdiyantini; Sri Pujiyanto
Jurnal Akademika Biologi Vol. 7 No. 4 Oktober 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Nilam (Pogostemon cablin) merupakan salah satu tanaman obat yang dapat digunakan sebagai antimikroba karena dapat menghasilkan minyak atsiri. Nilam juga dapat digunakan sebagai sumber isolat fungi endofit yang dapat dikembangkan sebagai alternatif penghasil senyawa antimikroba. Penelitian ini bertujuan untuk menyeleksi isolat fungi endofit tanaman nilam yang berpotensi sebagai antimikroba serta mengetahui aktivitas antimikroba supernatan dan ekstrak fungi endofit tanaman nilam terhadap bakteri Escherichia coli dan Staphylococcus aureus. Penelitian ini menggunakan rancangan penelitian Rancangan Acak Lengkap (RAL). Pengujian aktivitas antimikroba dari supernatan dan ekstrak fungi endofit (konsentrasi 10 µg/disk, 30 µg/disk, dan 50 µg/disk) dilakukan pada isolat E1, E2 dan E3 terhadap bakteri E. coli dan S. aureus. Perlakuan dilakukan tiga kali ulangan. Data dianalisis menggunakan One Way Anova dan uji lanjut Tukey. Sebanyak 3 isolat terbaik dipilih dari 14 isolat yaitu isolat E1, E2, dan E3. Hasil uji aktivitas antimikroba dari supernatan menunjukkan bahwa supernatan E2 memberikan diameter zona hambat paling besar terhadap E.coli (20.7 mm) dan S. aureus (19 mm) dibandingkan dengan supernatan E1 dan E3. Hasil uji aktivitas antimikroba dari ekstrak menunjukkan bahwa ekstrak E2 memberikan diameter zona hambat paling besar terhadap E.coli (18.5 mm) dan S. aureus (25.1 mm). Hasil uji Anova menunjukkan bahwa masing-masing supernatan terdapat pengaruh yang berbeda signifikan (p<0,05) dan masing-masing konsentrasi ekstrak pada isolat E1 dan Isolat E2 memiliki pengaruh yang berbeda signifikan (p<0,05) sedangkan ekstrak isolat E3 tidak memiliki pengaruh yang berbeda signifikan terhadap pertumbuhan bakteri E.coli dan S. aureus. 
ISOLASI, KARAKTERISASI Aeromonas hydrophila DAN DETEKSI GEN PENYEBAB PENYAKIT MOTILE AEROMONAS SEPTICEMIA (MAS) DENGAN 16S rRNA DAN AEROLYSIN PADA IKAN LELE (Clarias sp.) M Muslikha; Sri Pujiyanto; Siti Nur Jannah; Hessy Novita
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aeromonas hydrophila merupakan bakteri Gram negatif yang banyak ditemukan di perairan dan dapat menyerang ikan. Selain itu, A. hydrophila menyebaban penyakit Motile Aeromonas Septiemia (MAS) yang menyerang beberapa organ dalam seperti hati, limpa dan ginjal. Isolat bakteri diisolasi dari ikan lele (Clarias sp.) yang berasal dari berbagai daerah seperti, Ciganjur, Sukamandi dan Citayam. Penelitian ini bertujuan untuk mengisolasi, karakterisasi dan deteksi gen patogen bakteri A. hydrophila penyebab penyakit MAS pada ikan lele. Beberapa pengujian yang dilakukan pada penelitian ini yaitu uji biokimia (pewarnaan Gram, uji oksidatif-fermentatif, uji katalase, uji oksidase, uji d-mannitol, uji TSA skim milk, uji Mac Conkey, dan uji novobiosin), uji deteksi gen patogen dilakukan secara molekuler dengan menggunakan primer 16S rRNA dan aerolysin. Berdasarkan hasil pengujian biokimia, A. hydrophila merupakan Gram negatif dengan sel berbentuk basil pendek, bersifat motil, positif menghasilkan enzim oksidase, enzim katalase dan positif oksidatif dan fermentatif, positif fermentasi laktosa. Hasil deteksi gen patogen menunjukkan isolat AH2 dan B9 memiliki gen faktor virulen yaitu, aerolysin. Isolat AH2 dan B9 menghasilkan gen aerolysin kembali pada deteksi gen hasil reisolasi dari postulat Koch. Kata kunci : Ikan Lele (Clarias sp.), Aeromonas hydrophila, Gen Faktor Virulen, Motile Aeromonas Septicemia (MAS).
ISOLASI DAN IDENTIFIKASI BAKTERI GENUS Sphingomonas DARI DAUN PADI (Oryza sativa) DI AREA PERSAWAHAN CIBINONG Gabriela Christy Sabbathini; Sri Pujiyanto; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The unique ability of  the genus Sphingomonas bacteria as degrade the contaminants refractory contaminants, to serve as the antagonists bacteria to phytopathogenic fungi, and capable to secrete  hidhly useful exopolysaccharide gellan make these bacteria may play an important role in various industrial fields. Exploitation of the metabolic capabilities by genus Sphingomonas bacteria can provide significant commercial advantages for biotechnology.The species of Sphingomonas are often found associated with the rice plant as one of the endophytic bacteria that can be cultured. This study aims to isolate the local bacteria that can produce gellan gum from the leaves of the rice plant (Oryza sativa). The isolation process is done with a spread plate method suspension of rice leaves on Nutrient Dextrose Agar (NDA) media. Single colonies of bacteria that can be isolated then identified by colony PCR method to proceed at sequencing process. Sequencing followed by equalization sequences on the BLAST program shows four isolates of the genus Sphingomonas which isolates XA1, XA2, XA6, XA12 with the results are Sphingomonas sp. Fse41, Sphingomonas sp. Fse41, Sphingomonas sanguinis L4-317 strain and Sphingomonas sp. MLB01Keywords: endophytic bacteria, padi, Sphingomonas
Produksi Enzim Inulinase Khamir Pichia manshurica DUCC Y-015 Dari Tepung Umbi Dahlia (Dahlia variabilis Willd.) Dengan Variasi Konsentrasi Magnesium Sulfat (MgSO4.7H2O) Dan Waktu Inkubasi Riza Laksita Devi Mutiaratri; W Wijanarka; Sri pujiyanto
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Dahlia tubers (Dahlia variabilis Willd.) contain inulin which can be hydrolyzed by the inulinase enzyme (E.C.3.2.1.7) into fructose monomer units. Application of  inulinase enzyme is used in the production of HFS (High   Fructose  Syrup)  dan  FOS  (Fructo-oligosaccharides).  Inulinase  can  be  produced  by  several microorganisms  including  inulinolytic  yeast  Pichia  manshurica  DUCC  Y-015.  One  of  the  factors  that influence the production of enzyme inulinase is macro minerals and incubation time on production medium. This study aims to determine the concentration of magnesium sulphate (MgSO4.7H2O) and the most optimal incubation time in producing the inulinase  enzyme. The research was carried out experimentally using a Randomized Block Design factorial. The first factor is the concentration MgSO4.7H2O those are 0,5 g/L; 1 g/L; and 1,5 g/L. The second factor is the variation of the incubation time, those are 12 hours; 18 hours; and24 hours, repetition was performed three times. Data were analyzed using ANOVA with 5% significant level(α = 0,05) and Duncan Test for further analysis. The results showed that the variation of the concentration ofMgSO4.7H2O has not been able to increase the production of inulinase enzyme, while the incubation time of18 hours produced the inulinase enzyme activity of 0,9605 IU/mL. Keywords:  Inulinase,  Dahlia  variabilis  Willd.,  Pichia  manshurica  DUCC  Y-015,  MgSO4, Incubation Time
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI ANTAGONIS TERHADAP Vibrio parahaemolyticus PATOGEN PADA UDANG Litopenaeus vannamei DARI PRODUK PROBIOTIK DAN SEDIMEN MANGROVE DI REMBANG Bunga Fajriani; Anto Budiharjo; Sri pujiyanto
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Shrimp is one of the main commodities in the aquaculture industry because it has high economic value and high demand product. The Litopenaeus vannamei shrimp have the advantage of being able to grow as fast as a tiger shrimp (3 g / wk), can be grown on a wide salinity range (0.5-45 ppt), lower protein requirement (20-35%) than tiger shrimp and stylirostris shrimp. Vibrio parahaemolyticus is a normal flora in the brackish waters environment which is pathogenic to shrimp commodities as well as in humans. The use of Probiotics as additional feed in the form of microbial cells intact widely used in shrimp farming as one effort to improve the quality of the environment and supress the growth of pathogenic bacteria. One of the probiotic products used is super PS. This study aims to obtain bacterial isolate probiotic products that can suppress the growth of Vibrio parahaemolyticus bacteria. Isolation is also done on mangrove sediments as a comparable type of bacteria that can suppress the growth of pathogens. Methods used include isolation of bacterial probiotic and mangrove sediments, antagonistic test, and molecular identification with PCR methods (Polymerase Chain Reaction). The isolation result obtained by seven isolate bacteria of probiotic product and eleven isolate of mangrove sediment bacteria. One selected bacterial isolate from isolation of probiotic product that is IP 7 which able to supress the growth of Vibrio parahaemolyticus with a diameter of clear zone 10.84 mm. The results of identification using the 16S rRNA gene sequence showed that IP 7 isolate had a similarity index of 92% with Lysinibacilllus cresolivorans.Keyword : Litopenaeus vannamei, Vibrio parahaemolyticus, probiotics, antibacterial, gen 16S rRNA.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK TUMBUHAN Euphorbia hirta L. TERHADAP Ralstonia solanacearum, Escherichia coli, DAN Staphylococcus aureus SECARA IN VITRO Genoveva Preta Angelika; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biocontrol using Patikan kebo (Euphorbia hirta L.) plant is an alternative solution to control pathogenic bacteria. Such wild plant is known to contain active compounds with antibacterial activity such as tannins, alkaloids, flavonoids, saponins, and phenols. This study aims to determine the antibacterial activity of the methanol extract of E. hirta against R. solanacearum, E. coli and S. aureus. The extraction method of E. hirta was maceration with methanol solvent, while antibacterial activity test using the agar diffusion method (Kirby-Bauer) with test bacteria was R. solanacearum, E. coli and S. aureus. E. hirta extract tested was pure extract (100%). Observed response was diameter of inhibitory zone formed around the paper discs that had been smeared with E. hirta extract on the media. Analysis of the data using a Completely Randomized Design (CRD) 1 factor (test bacteria) with three times repetition, followed by a further test of Duncan with 95% confidence level. The results indicated that E. hirta produced extraction yield of 6,45%. Antibacterial activity of E. hirta extract against R. solanacearum, E. coli and S. aureus was indicated by Inhibitory Zone Diameter (HZD), respectively for 21,8 mm, 18,26 mm and 17,06 mm. The results of this study showed that the methanol extract of E. hirta plant had antibacterial activity against R. solanacearum, E. coli and S. aureus, thus can be used as a biocontrol agent of bacterial wilt disease in plants caused by R. solanacearum and human disease caused by E. coli and S. aureus. Keywords: Euphorbia hirta, Ralstonia solanacearum, Escherichia coli, Staphylococcus aureus, antibacterial activity, diffusion method
KAJIAN AWAL POTENSI OPOSUM LAYANG (Petaurus breviceps) SEBAGAI RESERVOIR BAKTERI ZOONOTIK DAN RESISTENSI ANTIMIKROBA Rifka A. N. Safitri; Sarsa A. Nisa; Nurul Inayah; Taufiq P. Nugraha; Agung Suprihadi; Sri Pujiyanto; Anang S. Achmadi; Achirul Nditasari; Sugiyono Saputra
BERITA BIOLOGI Vol 20, No 1 (2021)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i1.3974

Abstract

Oposum layang atau sugar glider (Petaurus breviceps) merupakan salah satu satwa endemik Indonesia. Permintaan akan satwa eksotis ini sebagai hewan peliharaan terus meningkat namun informasi terkait potensi zoonosis yang ditimbulkannya masih sangat terbatas. Penelitian ini bertujuan untuk mendeteksi dan mengidentifikasi bakteri patogen yang dibawa oleh oposum layang melalui pendekatan culture-dependant method dan untuk mengetahui pola resistensi antibiotiknya. Sampel yang digunakan adalah feses oposum layang (n=21) yang dikoleksi dari fasilitas riset satwa liar di Pusat Penelitian Biologi LIPI, Bogor. Berdasarkan uji presumptif Salmonella pada medium Xylose Lysine Deoxycholate (XLD) agar, sebanyak 6 sampel (29%) dinyatakan positif, sedangkan  uji presumtif untuk Listeria pada Listeria isolation transwab dinyatakan positif untuk semua sampel (100%). Secara total, sebanyak 43 isolat telah berhasil dikoleksi dan dikarakterisasi fenotipiknya terhadap antibiotik dan sembilan isolat (21%) diantaranya menunjukkan adanya resistensi terhadap satu jenis antibiotik atau lebih. Sementara itu, tiga isolat potensial patogen telah diidentifikasi menggunakan gen 16S rRNA yaitu Shigella sonnei (X15), Klebsiella pneumoniae (X21) dan Bacillus flexus (H8). Penelitian lanjutan untuk mengidentifikasi bakteri yang dikoleksi dan mengkonfirmasi patogenisitasnya masih perlu dilakukan namun berdasarkan hasil dari kajian awal ini, kami menguatkan hipotesis bahwa oposum layang berpotensi sebagai reservoir dari bakteri zoonosis sekaligus reservoir dari resistensi antimikroba. 
Isolasi kapang endofit dari tanaman ciplukan (Physalis angulata L.) dan potensi antibakteri terhadap Escherichia coli dan Staphylococcus aureus Wahyu Aji Mahardhika; MG Isworo Rukmi; Sri Pujiyanto
NICHE Journal of Tropical Biology Vol. 4, No. 1, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.1.33-39

Abstract

Isolasi khamir penghasil enzim inulinase dari buah kersen (Muntingia calabura) serta pengaruh mikronutrien mangan (Mn) pada produksi enzimnya Fitri Ulfana Risky; Wijanarka Wijanarka; Sri Pujiyanto
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5252.206 KB) | DOI: 10.14710/niche.2.2.27-37

Abstract

Produksi Pigmen Karotenoid oleh Khamir Phaffia rhodozyma yang Diperlakukan dengan Radiasi Sinar UV Sri Pujiyanto; Wijanarka Wijanarka; Endang Kusdiyantini; T. A. Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.146

Abstract

Carotenoid pigment is an essential element in aquaculture, since it gives characteristic of color on shrimp and fish. Carotenoid pigments can be produced microbiologically using Paffia rhodozyma. Genetic improvement of the yeast, one of which can be accomplished by radiation mutation, will increase the production of carotenoid pigments. The aims of this study were to mutate P. rhodozyma using UV irradiation and to figure out pigment production by the mutant strains resulting from 30 minute-irradiation. Irradiated culture was incubated in dark condition and plated onto YMA media. Grown mutant colonies were collected in order to test for their pigment production. Pigment production was measured on the basis of extinction coefficient of 1%. The results showed that mutant strain encoded with MUV-1 produced the highest pigment at 179.96 mg/g dry weight cell, higher than the wild type (63.20 mg/g dry weight cell).
Co-Authors Achirul Nditasari Agung Suprihadi Agung Suprihadi Ahmad Qi Sahlan Anang S. Achmadi Anggraeni, Via Annisa Widyasari Anto Budiharjo Aqlina, Maulida Ari Wibawa Budi Santosa Arina Tri Lunggani Ariyani, Mei Dwi Aviany, Hanna Berliana Bambang Sri Waluyo Budi Raharjo Budi Raharjo Bunga Fajriani Chang, Helen Choirunnisaa, Nadia Maharani Jasmine Debby Widiyanti Dewanti, Ardelia Dewi, Tirta Kumala Dewi, Yulita Wiwik Irana Diani Ajeng Prahesti Dinda Khairunnisa, Dinda Dwi Retnowati Elawati, Nunung Eni Elawati, Nunung Eni Endang Kusdiyantini Endang Kusdiyantini Erfianti, Tia Fahrurrozi Fahrurrozi Fathmah, Ema Nuzula Fathmah, Ema Nuzulah Fatin, Nuhaul Fitri Ulfana Risky Fitria Fitria Gabriela Christy Sabbathini Genoveva Preta Angelika Ghaida Afra Akhsani Halwiyah, Nurul Hapzi Ali Hermin Pancasakti Kusumaningrum Hery Widijanto Hessy Novita Jepri Agung Priyanto, Jepri Agung Larasati, Dinar Rahmi Luthfian Nur Afifi M Muslikha Maerani Sumarno Mamik Setyowati Maulida Aglinia Mawarni, Shelina Nurunnisa MG Isworo Rukmi Moi, Maria Yasinta Mulyani, Nies S Nadina, Rahmah Qisti Nafisah, Hidayatun Nandina, Rahmah Qisti Nanik Rahmani NANIK RAHMANI Novi Alvita Pratama Noviasih, Ria Frasida Nunung Eni Elawati Nunung Eni Elawati Nurul Inayah Oktavia, Anik Oktavia, Nurrizqi Permatasari, Vera Pramita Dian Pramitasari Prastya, Muhammad Eka Pratama, Novi Alvita Primahana, Gian Putra, Mohammad Affan Dwica Putri Ramadhani Putri, Adde Lolita Octavia R. Rahardian Rahmah, Chairunnisaa Jabal Rahmandanni, Yunnia Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi RATIH DEWI HASTUTI Rejeki Siti Ferniah Resdiani, Merysa Ridho Mathori Ikhwan Rifka A. N. Safitri Ristia Rachmatunnisa Riza Laksita Devi Mutiaratri Rizko, Nurmalisa Roseliana Fitri Roslenawati Roslenawati RS Ferniah Sari, Suli Arum Sarsa A. Nisa Setiawan, Ruby Sigit Hananto Siti Nur Jannah Siti Nur Jannah Sri Rahayu Tri Astuti Sugiyono Saputra Sumarno, Maerani Sunarno s Sunarno Sunarno Sunarno Sunarno Suprihadi Supriyadi Supriyadi Susiana Purwantisari T. A. Lestari Taufiq P. Nugraha Tri Rahayu, Hesti Tsania Dyna Falasifa Utami, Linda Ayu w wijanarka Wahyu Aji Mahardhika Wandita, Ryan Hilda Wigunarti, Anggia Hesti Wijanarka Wijanarka Wijanarka, W Wijarnaka, Wijarnaka Yopi Yopi YOPI YOPI Yudi Yunanto Yuswan, Apriza