Djoko Purnomo
Program StudiAgroteknologiFakultasPertanian UNS Jl. Ir. Sutami, Kentingan, Surakarta

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In Vitro Proliferation and Acclimatization of Kepok Banana Unti Sayang (ABB) with Addition of Organic Materials Rachmi, Devinawati; Samanhudi; Purnomo, Djoko
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.91-100

Abstract

Pisang kepok (Musa balbisiana) mengandung genom B dengan potensi pencoklatan tinggi dan tingkat proliferasi rendah dalam kultur in vitro. Salah satu cara mengurangi pencoklatan dan meningkatkan kandungan vitamin serta hormon dalam media, yaitu dengan penambahan suplemen sintetik maupun ekstrak bahan organik alami. Penelitian ini bertujuan mengetahui proliferasi eksplan pisang kepok Unti Sayang dengan media diperkaya ekstrak buah dan kemampuan aklimatisasi planletnya. Penelitian ini terdiri atas 2 percobaan, pertama tahap in vitro menggunakan Rancangan Acak Lengkap split plot dengan 2 faktor, yaitu main plot (3 taraf suhu ruang kultur) dan subplot (5 taraf ekstrak buah). Kedua, tahap aklimatisasi menggunakan Rancangan Acak Lengkap split plot dengan 2 faktor, yaitu main plot (4 taraf media aklimatisasi) dan subplot (4 taraf vigor planlet). Hasil percobaan menunjukkan penambahan ekstrak buah pada media MS berpengaruh, sedangkan perbedaan suhu inkubasi tidak berpengaruh terhadap proliferasi eksplan pisang kepok Unti Sayang. Media MS dengan penambahan ekstrak buah pepaya (150 g L-1) dan air kelapa (100 ml L-1) memberikan hasil yang tidak berbeda nyata dengan penambahan vitamin sintetik terhadap proliferasi dan morfogenesis eksplan pisang kepok Unti Sayang. Vigor planlet pada berbagai media aklimatisasi tidak berbeda nyata dengan kemampuan hidup mencapai 92%.
Study of Black Rice Parents Performance and the Crossing Ability Nandariyah, Nandariyah; Sukaya, Sukaya; Purnomo, Djoko; Sutarno, Sutarno; Yuniastuti, Endang; Az-Zahra, Catalina Dara Ayu
Caraka Tani: Journal of Sustainable Agriculture Vol 38, No 1 (2023): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v38i1.60245

Abstract

Black rice generally has problems in cultivation such as relatively long plant life, high habitus and low productivity. Plant breeders use several methods in hybridization activities, such as backcross method that can lead to the development towards emphasizing the superiority of each parent. This research aimed to study and determined the success rate of the black rice lines F1 backcross and the parents’ performance. This research was conducted using F1 of promising lines, parental lines and the Jeliteng variety with 9 sets of crosses. Each line and variety were repeated 3 times, in total there were 57 experimental units. The observation parameters of this research were plant height, crossing success, weight of seeds, length and width of grain, flowering age, harvesting age also number of grains and unfilled spikelet. The results of this study showed that parents plant height was positively correlated with the number of productive tillers. The taller the plant, the more productive tillers and the more flowers can be crossed. The success of the cross can increase with the number of flowers crossed. The success of crosses between F1 black rice promising lines and their parents has a success rate range of 10.82% to 33.75%. The findings imply that F1 crossbreeding of black rice promising lines with their parents can be carried out to produce backcross offspring.
Effect of NPK Fertilizer and Foliar Fertilizer on Chili Growth and Yield Anatalia, Rahma; Harsono, Puji; Yunindanova, Mercy Bientri; Purnomo, Djoko
Agrotechnology Research Journal Vol 6, No 2 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.858 KB) | DOI: 10.20961/agrotechresj.v6i2.54540

Abstract

Chili consumption in Indonesia has increased. Efforts to increase chili production need fertilization through roots and leaf fertilization. The research aimed to obtain optimum intervals of NPK (nitrogen, phosphor, potassium) fertilization and kinds of foliar fertilizer for the growth and yield of chili. The research was conducted from April to August 2020 in Gayam Village, Sukoharjo Regency, Central Java, Indonesia. This study used an experiential method with a Randomized Complete Block Design (RCBD) of 2 factors. The first factor was an interval of NPK fertilization (16-16-16) with a concentration of 25 g.L-1 with a dose of 240 mL.plant-1. The interval of NPK fertilization consists of 4 levels, NPK fertilizer which was applied every 5, 10, 15, and 21 days. The second factor was foliar fertilizer which consists of A foliar fertilizer, B foliar fertilizer, and C foliar fertilizer. The results showed that the interval of NPK fertilizer affected the number of dichotomous branches, dry stove weight, cumulative fruit, cumulative fruit weight, and fruit length at 1st harvest. NPK fertilization every 15 days increased the dichotomous branches by 23.16%, dry stove weight by 37.98%, cumulative fruits by 37.41%, and cumulative fruit weight by 41.46%. B foliar fertilizer and A foliar fertilizer gave the same performance in dichotomous branches. There was no interaction effect between the interval of NPK fertilization and the kind of foliar fertilizer on all growth and yield variables. NPK fertilization optimum intervals as supplementary fertilizers should be given every 15 days. This research can be used as a reference for farmers in fertilizing chilies.