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All Journal Jurnal Fitopatologi Indonesia Jurnal BETA (Biosistem dan Teknik Pertanian) GEMA TEKNOLOGI Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Vektora : Jurnal Vektor dan Reservoir Penyakit VISIKES Prosiding KPSDA Planta Tropika Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA E-Dimas: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Jurnal Surya Masyarakat Jurnal Entomologi Indonesia To Maega: Jurnal Pengabdian Masyarakat Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Surya Masyarakat Bioscientist : Jurnal Ilmiah Biologi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Pengabdian Vokasi Biosaintifika: Journal of Biology & Biology Education E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian Hasil Hutan Jurnal Bioteknologi & Biosains Indonesia

Susiana Purwantisari

Biotechnology Laboratory, Departement Of Biology, Faculty Of Science And Mathematics, Diponegoro University

Author-ID : 349902

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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HUBUNGAN HIGIENE SANITASI DENGAN KUALITAS MIKROBIOLOGIS PADA MAKANAN GADO-GADO DI KECAMATAN TEMBALANG KOTA SEMARANG Yuniatun, Tuti; Martini, Martini; Purwantisari, Susiana; Yuliawati, Sri
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 4 (2017): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Gado-gado is one of traditional foods from Indonesia which sold in Tembalang District. Gado-gado is made from various vegetables mixed with peanut sauce, served at temperature 5-60oC which is the "temperature danger zone" for any food. This situation makes gado-gado such a watery yet not-so-hot food when its served, so has a potency to be contaminated by microbes and fungis. The purpose of this research is to understand correlation between hygiene sanitation and microbiological quality of gado-gado in Tembalang District, Semarang City. This research is observational research using cross sectional design and consists of three variables, namely sellers health condition, sellers hygienic practice, and places sanitation. The samples of this research are 36 gado-gado and respondents of this research are 36 gado-gado sellers which is observed their hygienic sanitation while processing gado-gado. Data were analyzed by frequency distribution and bivariate analysis using chi square. The results showed that sellers health condition categorized as good condition (88,9%); sellers hygienic practice categorized as good condition (33,3%); and places sanitation categorized as good condition (22,2%). There was statistically significant correlation between sellers hygienic practice (p=0,008) and places sanitation (p=0,028) with gado-gado microbiological quality. While there is no corrrelation between sellers health condition (p>0,999) with gado-gado microbiological quality.Gado-gado sellers should pay attention to their personal hygiene and the places sanitation when processing gado-gado, to improve gado-gado microbiological quality.

HUBUNGAN PRAKTIK HIGIENE PENJAMAH DENGAN KUALITAS MIKROBIOLOGIS PADA JAJANAN SIOMAY DI KECAMATAN TEMBALANG KOTA SEMARANG Azizah, Hana Fitria; Martini, Martini; Purwantisari, Susiana
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 4 (2017): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Siomay is a mixed food that has a limit of colony growth 1 x 104 colony/g or mL. A fish or meat-based food such as siomay is easily contaminated by pathogenic bacteria such as Escherichia coli. The contamination on siomay could be caused by the hygiene practices of food that was not applied properly. This research aimed to analyze the association between hygiene practices and microbiological quality of siomay stall in Tembalang Sub-District. The type of this research was analytic observasional study with cross-sectional approach. The population of this research was all of the siomay stall in Tembalang Sub-District. Sampling procedure used accidental sampling technique and it was obtained 37 samples. Interview and observation were conducted on hygiene practices, raw materials quality and raw materials storage practices at siomay stall. The data were analysed using chi square test. The results revealed that samples of 73% had the MPN values exceeding the limit, 70,3% was contaminated Escherichia coli exceeding requirement, and 32,4% was grown yeast exceeding the standards, then 91,9% samples did not supply the microbiological quality of food. The analysis showed that there was an association between hygiene practice (p = 0,047) in siomay stall with the microbiological quality. However, the raw materials quality and the raw materials storage practices of siomay had been quite good.

HUBUNGAN SANITASI DENGAN STATUS BAKTERIOLOGI (STATUS Koliform DAN KEBERADAAN Salmonella sp) PADA JAJANAN DI SEKOLAH DASAR WILAYAH KECAMATAN TEMBALANG, SEMARANG Kumalasari, Ririh Citra; Martini, Martini; Purwantisari, Susiana
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 3 (2016): MEI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Foodborne disease adalah suatu penyakit yang disebabkan oleh adanya mikroorganisme patogen yang masuk bersama makanan. Produk pangan yang tidak memenuhi persyaratan mutu, keamanan dan cemaran bakteri patogen dapat mengakibatkan terjadinya foodborne disease. Anak sekolah merupakan usia yang rentan terhadap infeksi bakteri dan membutuhkan makanan yang cukup secara kuantitas serta kualitas sehingga memiliki keadaan atau status gizi yang baik dan dapat memperkuat sistem imun dalam tubuhnya. Penelitian ini bertujuan untuk mengetahui hubungan sanitasi dengan kontaminasi bakteri pada makanan jajanan di kantin sekolah dasar yang berada Kecamatan Tembalang Kota Semarang. Jenis penelitian analitik observasional dan pendekatan cross sectional. Sampel penelitian sejumlah 48 jajanan yang dijual di sekolah dasar. Pemeriksaan kontaminasi bakteri didasarkan angka koliform dan Salmonella sp. Data dianalisis dengan Chi-squre test. Hasil penelitian menunjukkan jajanan di kantin sekolah dasar yang tidak memenuhi syarat kesehatan sebesar 82,2% dengan status koliform tidak memenuhi syarat sebanyak 67,8% dan terkontaminasi Salmonella sp sebanyak 37,8%. Faktor yang berhubungan dengan kontaminasi bakteri dan status bakteriologi jajanan di sekolah dasar diantaranya adalah pengetahuan penjual, praktik sanitasi penjual, serta sanitasi tempat berjualan. Pendidikan kesehatan tentang pengelolaan makanan perlu diberikan pada penjual makanan di sekolah dasar.

KARAKTERISASI BIODEGRADASI SENYAWA POLIAROMATIK DIBENZOTHIOPHENE OLEH BAKTERI LAUT NOVOSPHINGOBIUM MATHURENSE LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2894

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic

EKSTRAKSI SENYAWA BIOAKTIF THEAFLAVIN TEH OOLONG (CAMELLIA SINESIS) DENGAN AIR SEBAGAI GREEN SOLVENT Amalia, Rizka; Yulianto, Mohamad Endy; Purwantisari, Susiana; Yudanto, Yusuf Arya; Pangestu, Ilyas Teguh
Gema Teknologi Vol 21, No 1 (2020): April 2020 - October 2020
Publisher : Vocational School Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/gt.v21i1.32287

Theaflavin, a polyphenolic compounds foundÂ in oolong tea and black tea, thought to have medicinal potency. Theaflavins and their derivatives such as theaflavin gallate have shown a broad spectrum of antiviral activity against several viruses, including influenza A, B and hepatitis C viruses. Previous research show that theaflavins could inhibit RdRp activity through blocking the active site in the catalytic pocket ofÂ RdRpÂ in SARSâ€CoVâ€2, SARSâ€CoV and MERSâ€CoV.. This research aims to extract theaflavins in oolong tea with water solvent at a temperature of 100oC, pressure > 1 atm and varied extraction times (10-60 minutes). The effect of oolong tea extraction time towards theÂ theaflavin content was observed. The results showed that with the longer extraction time, the theaflavin levels were reduced due to the degradation of thermal theaflavins into thearubigins. The model exponential equation obtained is Â y=14,91488 e-0,2631x + 0,11865, with the R2=0.99161. The best operating conditions was obtained at 10 minutes of extraction time, resulted in 1.19% of the total theaflavin content of oolong tea.

Viabilitas Biofungisida Produk Lokal dan Aplikasinya untuk Penundaan Gejala Penyakit Hawar Daun Tanaman Kentang Naufal, Mochammad Faâ€™iq Qoys; Purwantisari, Susiana
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.188-195

Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of Trichoderma agent.Â The research objective was to determine the ability of biofungicide product from Temanggung local farmer group to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at Kaponan Â Sub District, Pakis District and Magelang Regency. Complete Randomized DesignÂ with six treatments and twenty replication was applied and each of which was placed in a different plot. On the first plot treatment was given chemical fertilizer application (P0);The second plot treatmentÂ (P2) was given with leaking and spraying application of biofungicide ( H-7, H+8, and H+15); The third plot treatment (P3) was given with spraying application of biofungicideÂ Â (H+8, H+15, and H+21); The fourth plot treatment (P4) was given with spraying application of biofungicideÂ Â (H+8, H+15, H+21 and H+28), and the last tretment (P5) was given with no application of chemical fungiside and pesticide. Result of the research showed that application of biofungicide could delay late blight disease incubation period until 14 days compared the control treatment (P0). The percentage of viability of the active biofungicide after being stored for 4 months still shows a high value of 87.13%, so that this local product biofungicide still meets the feasibility standard for its application in the field.

Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity Purwantisari, Susiana; Sitepu, Harum; Rukmi, Isworo; Lunggani, Arina Tri; Budihardjo, Kadarwati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26706

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.

POPULASI VEKTOR DEMAM BERDARAH DENGUE DI DAERAH RURAL KOTA SEMARANG : CROSSECTIONAL SURVEY BIONOMIK AEDES sp Sri Yuliawati; Dewi Fajar Kharisma; Martini Martini; Lintang Dian Saraswati; Retno Hestiningsih; Susiana Purwantisari
VISIKES: Jurnal Kesehatan Masyarakat Vol 19, No 2 (2020)
Publisher : Dian Nuswantoro Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/visikes.v19i2.3659

Dengue Hemorrhagic Fever (DHF) caused by dengue virus transmitted by Aedes sp. Population of Aedes sp needed to know bionomic and ecologic especially in rural areas. This study aims to determine the Aedes sp density description at the Rowosari village, Tembalang Sub-district, Semarang City. This is descriptive research using cross sectional approach that conducted by survey and observation method about the density of Aedes sp. The study sample are 100 houses spreading in each sub village in Rowosari selected by the purposive sampling method. The results show the density of larvae in Rowosari is very high based on larvae density index HI = 44.1%, CI = 31,7%, BI =74,9% OI= 64,1%, so it can be said Rowosari including areas with high risk of dengue. The Aedes aegypti density is 0.67 resting inside house and 0 resting outside house. The Aedes albopictus density 0.13 resting inside house and 0,06 resting outside house. During the study founded 2 types of Aedes sp that are Aedes aegypti (53%) and Aedes albopictus (4.7%). Based on this research, suggest to use the type of container that mosquitoes doesn’t prefer for breeding place and routinely clean the containers either in house and outside the house.Keywords: DHF, Aedes sp, Aedes albopictus, Larvae Density, DBD

Penapisan Cendawan Trichoderma spp. untuk Pengendalian Phytophthora infestans secara in vitro Susiana Purwantisari; Achmadi Priyatmojo; Retno Peni Sancayaningsih; Rina Sri Kasiamdari
Jurnal Fitopatologi Indonesia Vol 12 No 3 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Late blight disease on potato caused by a plant pathogenic fungus (Phytophthora infestans) is the most important disease in Indonesia. The use of antagonist fungi Trichoderma is an environmentally friendly technology to control the potato disease. The purpose of this study was to determine the ability of Trichoderma spp. to control P. infestans in vitro. Trichoderma spp. have been isolated from suppressive soil at central potato areas in district of Lembang, Bandung, West Java Province. To determine the inhibition ability of Trichoderma spp against P. infestans, a dual culture method was performed. Variable observed was inhibition zone of Trichoderma spp. against P. infestans. The results showed that 9 isolates of Trichoderma were successfuly isolated from suppressive soil, i.e. T. viride (2 isolates), T. atroviride (1 isolate), T. harzianum (1 isolate) and T. aureoviride (5 isolates). All the Trichoderma isolates revealed growth inhibition abality against P. infestans and. The highest growth inhibition (68.6%) was observed by T. viride isolate ( Ti 9).

Estimasi Produksi Tandan Kelapa Sawit Berdasarkan Analisis Ketersediaan Air dengan Teknik Oil Palm Dissection Sukarman Sukarman; Herry Wirianata; Kadarwati Budiharjo; Septa Primananda; Susiana Purwantisari
Jurnal BETA (Biosistem dan Teknik Pertanian) Vol 9 No 2 (2021): September
Publisher : Program Studi Teknik Pertanian dan Biosistem, Fakultas Teknologi Pertanian, Universitas Udayana, Badung, Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBETA.2021.v09.i02.p16

Jika faktor genetik, tanah dan teknik budidaya bukan merupakan faktor pembatas, maka fluktuasi hasil panen dipengaruhi oleh faktor iklim atau lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh ketersediaan air (defisit air, curah hujan dan hari hujan) terhadap sex ratio termasuk model hasil panen kelapa sawit. Penelitian telah dilakukan pada bulan November 2016 hingga Oktober 2017 di wilayah Wilmar International Plantation Kalimantan Tengah. Analisis menggunakan regresi berganda dan uji korelasi termasuk uji asumsi klasik (uji normalitas, multikolinieritas dan heteroskedastisitas) untuk analisis statistik dalam penelitian ini. Hasil penelitian menunjukkan bahwa curah hujan dan hari hujan berpengaruh nyata terhadap perkembangan sex ratio selama 23 bulan sebelum panen (MBH), saat defisit air berpengaruh nyata terhadap sex ratio pada 24 MBH. Estimasi distribusi produksi bulanan, anggaran produksi dan produksi aktual menunjukkan bahwa tidak signifikan. Estimasi distribusi produksi bulanan, anggaran produksi dan realisasi produksi menunjukkan bahwa tidak signifikan, dapat digunakan keakuratan distribusi produksi bulanan kelapa sawit. Kata kunci: sex ratio, curah hujan, hari hujan, defisit hujan, bunga betina

Co-Authors Achmadi Priyatmojo Agus Evendi, Agus Ahmad Arfi Setiadi Ahmad Hanafi Ahmad Thontowi Akbar, Muhammad Falih Amal, Daffa Ikhlasul Amatullah, Lutfiana Hary Ana Bila Nadia Anindya Ardiansari Annisa Dhea Lathifa Anto Budiharjo Arfieta, Frisca Arina Tri Lunggani Astriana, Novi Atik Mawarni Azizah, Hana Fitria Bodhicitta Wardaja Budi Raharjo Budi Raharjo Budi Setiyana Budi Setiyana Budihardjo, Kadarwati Chatarina Umbul Wahyuni Choiriyah, Miftahul Dafip, Muchamad Dewi Fajar Kharisma Dion, Romario Dwi Handayani Dwi Handayani Eflita Yohana Elvi Yetti, Elvi Fikri, Dzakiyya Nabilla Fiva Andriyani Govinda Restu Syahputra Hapzi Ali Harum Sitepu Harwika Ria Rizky Agista Hermawan Dwi Ariyanto Hermawan Dwi Ariyanto Herry Wirianata Husna, Faza Laili Ilyas Teguh Pangestu Indriana Putri Inggrit Amedia, Inggrit Jaya, Myra Ivana Yulia Kadarwati Budiharjo Karnoto, K Khanifah, Neni Nur Lintang Dian Saraswati M. Arie Wuryanto Mahardhika, Wahyu Aji Martini Martini MG Isworo Rukmi Mohamad Endy Y Yulianto Mufida, Naimatul Muhammad Bismar Iskandar putra Naufal, Mochammad Faâ€™iq Qoys Noviyanti, Nita Nurbayani, Farah Arhusy Nurul Halwiyah Pangestu, Ilyas Teguh Purwoko, Agus Puspitasari, Elsye Giovanny Rahmadita, Tika Ramadhan, Akhmad Andika Rasyid, Rido Baharudin Ar Rejeki Siti fe Rejeki Siti Ferniah Retno Hestiningsih Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Rina Sri Kasiamdari Ririh Citra Kumalasari, Ririh Citra Risnanda, Meizulfa Ayu Rizka Amalia Rizky Wahyu Saputro Safina, Marista Fikri Irsya Sari, Dyah Maharani Sisya Puspita Sari, Rachel Dewita Sarjana Parman Septa Primananda Sitepu, Harum Siti Nur Jannah Siti Nur Jannah Sofatun Misrofah Sri Pujiyanto Sri Yuliawati Sri Yuliawati Sri Yuliawati Sukarman Sukarman Supriyadi Supriyadi Sutra Diyana Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Tatik Handayani, Tatik Theresia Damayanti Tuti Yuniatun, Tuti Wardana, Tegar Pratama Kayong Wibisono, Shafa’ Khairunnisa Wijanarka Wijanarka Wulandari, Sukma Aulia YOPI YOPI Yudanto, Yusuf Arya

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