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All Journal Jurnal Fitopatologi Indonesia Jurnal BETA (Biosistem dan Teknik Pertanian) GEMA TEKNOLOGI Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Vektora : Jurnal Vektor dan Reservoir Penyakit VISIKES Prosiding KPSDA Planta Tropika Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA E-Dimas: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Jurnal Surya Masyarakat Jurnal Entomologi Indonesia To Maega: Jurnal Pengabdian Masyarakat Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Surya Masyarakat Bioscientist : Jurnal Ilmiah Biologi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Pengabdian Vokasi Biosaintifika: Journal of Biology & Biology Education E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian Hasil Hutan Jurnal Bioteknologi & Biosains Indonesia
Susiana Purwantisari
Biotechnology Laboratory, Departement Of Biology, Faculty Of Science And Mathematics, Diponegoro University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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MOLECULAR IDENTIFICATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM PGPR PRODUCT DERIVED FROM TEMANGGUNG REGENCY Akbar, Muhammad Falih; Purwantisari, Susiana; Pujiyanto, Sri; Ferniah, Rejeki Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2840

Abstract

T Phosphate solubilizing bacteria are soil bacteria that can dissolve phosphates from insoluble phosphate bonds so that they can be absorbed by plants. Phosphates in the soil are naturally present in organic and inorganic forms. Both forms are insoluble or slightly soluble forms of phosphate, so their availability for soil biota is very limited. This study aimed to identify the phosphate solubilizing bacteria from locally PGPR product from Temanggung, Indonesia, namely BPF 1. The method in this study was carried out by molecular identification using the 16S rRNA encoding gene which includes bacterial DNA isolation, DNA amplification using PCR, bacterial DNA sequencing and the creation of phylogenetic trees. The results of the study were obtained isolate bacteria solubilizing phosphate BPF 1 from PGPR products of local farmers of Temanggung Regency identified as the genus Bacillus, having the closest kinship with B. subtilis strain SBMP4 (NR_118383.1) with a similarity of 98.42%.
Characterization and Evaluation of Tobacco Stem Waste Liquid Smoke as an Eco-Friendly Tofu Bio-preservation Purwantisari, Susiana; Rasyid, Rido Baharudin Ar; Wardana, Tegar Pratama Kayong; Arfieta, Frisca; Wibisono, Shafa’ Khairunnisa; Jaya, Myra Ivana Yulia; Dafip, Muchamad
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.21552

Abstract

Tofu preservation often uses unsafe chemicals, especially formaldehyde, that trigger health problems in humans. Therefore, it is necessary to develop preservation methods that are both effective and safe for human health. Liquid smoke derived from tobacco stem waste is well known to contain antioxidant and antimicrobial properties that may be suitable for use as a preservative compound for tofu. This study aims to evaluated the potential of tobacco stem liquid smoke as a natural preservative by analyzing its chemical profile, antimicrobial efficacy, and impact on tofu characteristics. Tofu samples were soaked in 0%, 5%, and 15% liquid smoke solutions and stored at room temperature for 24 hours. GC-MS analysis confirmed the presence of key compounds: phenolics (51.21%), fatty acids (30.82%), and ketones (17.96%). The treatment significantly improved preservation metrics, reducing the microbial load by up to 81% and lowering the pH to 5.1. The moisture content was reduced while protein levels were proportionally maintained. The 15% concentration provided the highest antimicrobial effect, enhanced aroma, and imparted a distinctive smoky flavor, yet the treatment was preferred by panelists for its better preservation of original color and soft texture. These findings highlight the potential of tobacco stem liquid smoke as a natural, low-cost preservative for tofu, promoting the valorization of biomass waste into value-added products.
Co-Authors Achmadi Priyatmojo Agus Evendi, Agus Ahmad Arfi Setiadi Ahmad Hanafi Ahmad Thontowi Akbar, Muhammad Falih Amal, Daffa Ikhlasul Amatullah, Lutfiana Hary Ana Bila Nadia Anindya Ardiansari Annisa Dhea Lathifa Anto Budiharjo Arfieta, Frisca Arina Tri Lunggani Astriana, Novi Atik Mawarni Azizah, Hana Fitria Bodhicitta Wardaja Budi Raharjo Budi Raharjo Budi Setiyana Budi Setiyana Budihardjo, Kadarwati Chatarina Umbul Wahyuni Choiriyah, Miftahul Dafip, Muchamad Dewi Fajar Kharisma Dion, Romario Dwi Handayani Dwi Handayani Eflita Yohana Elvi Yetti, Elvi Fikri, Dzakiyya Nabilla Fiva Andriyani Govinda Restu Syahputra Hapzi Ali Harum Sitepu Harwika Ria Rizky Agista Hermawan Dwi Ariyanto Hermawan Dwi Ariyanto Herry Wirianata Husna, Faza Laili Ilyas Teguh Pangestu Indriana Putri Inggrit Amedia, Inggrit Jaya, Myra Ivana Yulia Kadarwati Budiharjo Karnoto, K Khanifah, Neni Nur Lintang Dian Saraswati M. Arie Wuryanto Mahardhika, Wahyu Aji Martini Martini MG Isworo Rukmi Mohamad Endy Y Yulianto Mufida, Naimatul Muhammad Bismar Iskandar putra Naufal, Mochammad Fa’iq Qoys Noviyanti, Nita Nurbayani, Farah Arhusy Nurul Halwiyah Pangestu, Ilyas Teguh Purwoko, Agus Puspitasari, Elsye Giovanny Rahmadita, Tika Ramadhan, Akhmad Andika Rasyid, Rido Baharudin Ar Rejeki Siti fe Rejeki Siti Ferniah Retno Hestiningsih RETNO PENI SANCAYANINGSIH Retno Peni Sancayaningsih Rina Sri Kasiamdari Ririh Citra Kumalasari, Ririh Citra Risnanda, Meizulfa Ayu Rizka Amalia Rizky Wahyu Saputro Safina, Marista Fikri Irsya Sari, Dyah Maharani Sisya Puspita Sari, Rachel Dewita Sarjana Parman Septa Primananda Sitepu, Harum Siti Nur Jannah Siti Nur Jannah Sofatun Misrofah Sri Pujiyanto Sri Yuliawati Sri Yuliawati Sri Yuliawati Sukarman Sukarman Supriyadi Supriyadi Sutra Diyana Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Tatik Handayani, Tatik Theresia Damayanti Tuti Yuniatun, Tuti Wardana, Tegar Pratama Kayong Wibisono, Shafa’ Khairunnisa Wijanarka Wijanarka Wulandari, Sukma Aulia YOPI YOPI Yudanto, Yusuf Arya