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Gambaran Kadar Kolesterol Total pada Pengguna Rokok Elektrik Dewi Arisanti; Mujahidah Basarang; Muh. Rifo Rianto; Waode Rustiah; Siti Maryam Bano
Lontara Journal of Health Science and Technology Vol 4 No 2 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v4i2.404

Abstract

An electric cigarette is a tool that functions to convert chemical substances into the form of vapor and distributes them to the lungs using electricity. One of the harmful elements contained in cigarettes is nicotine. If nicotine circulates in the body, it stimulates the secretion of the adrenaline hormone resulting in an increase in heart rate, blood pressure and changes in fat metabolism. This study aims to describe cholesterol levels in e-cigarette users in Mamajang sub-district. This research is a laboratory observation which aims to describe cholesterol levels in e-cigarette users in Mamajang district. The number of samples used were 20 samples. The object of this research is the blood of electric smokers using the POCT method. From the results of research conducted on 20 samples. 6 of them got increased results and 14 samples got normal. So it can be concluded that of the 20 samples, 6 samples (74%) got normal results and 6 samples (26%) got increased results.
Comparison of Carbol Fuchsin Concentration on the Results of Acid-Fast Bacteria Staining (AFB) Al Adawiah, Putri Rabiah; Basarang, Mujahidah; Rantung, Juneke Gabriella
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.691

Abstract

Background & Objective: Infections caused by the bacterium Mycobacterium tuberculosis are known as tuberculosis. (TB). The treatment process takes a long time because this bacillus bacteria is very strong. Compared to other parts of the human body, this bacteria more frequently attacks the lungs. Patients who test positive for acid-fast bacilli (AFB) are the cause of pulmonary tuberculosis (TB). The aim of this research is to determine how the concentration of Carbol Fuchsin differs in the results of Acid-Fast staining. (BTA). Method: This research was conducted in a laboratory and used purposive sampling techniques with the Ziehl-Neelsen staining method, resulting in 5 positive acid-fast bacilli (AFB) samples. Result: The results of this study indicate that the best concentration is 1.5% carbol fuchsin, so it can be concluded that the optimal staining concentration is 1.5% carbol fuchsin. Conclusion: Based on the research conducted, it was concluded that the higher the concentration of carbol fuchsin, the stronger it binds to the cell walls that have a lipid layer, thus being able to bind the red colour.
Identification of Malassezia sp. as a Cause of Dandruff Among Students at Muhammadiyah Polytechnic Makassar Annisa, Riska; Rasyid, Nur Qadri; Basarang, Mujahidah; Faisal, Restu Andini
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.703

Abstract

Background & Objective: Dandruff is a scalp condition attributed to the fungus Malassezia sp. It manifests as fine to coarse scales that are grayish or white, resulting from the shedding of the stratum corneum layer on the scalp. Factors contributing to the onset of dandruff include the proliferation of Malassezia fungi, sebaceous gland activity, and individual predisposition. This study aimed to identify Malassezia sp. responsible for dandruff among female students at the Muhammadiyah Polytechnic of Makassar. Method: The research employed a laboratory observational design utilizing a purposive sampling technique. Dandruff samples were collected from 10 female students and cultured on SDA (Sabouraud Dextrose Agar) media, followed by microscopic examination of the resulting colonies. Result: The findings revealed that none of the 10 dandruff samples contained Malassezia sp.; instead, contamination by Aspergillus fumigatus was observed. Conclusion: Therefore, it can be concluded that Malassezia sp. was not identified as a causative agent of dandruff in female students at the Muhammadiyah Polytechnic of Makassar
Kemampuan Senyawa Anti Ketombe pada Sampo dalam Menghambat Pertumbuhan Jamur yang Diisolasi dari Ketombe Basarang, Mujahidah; Rianto, Muh. Rifo; Julianti, Riskiah Dwi
Lontara Journal of Health Science and Technology Vol. 3 No. 2 (2022): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v3i2.320

Abstract

Dandruff is a medical condition that indicates the activity or growth of fungi on the scalp. Shampooing using an anti-dandruff shampoo is an action that can be taken to inhibit the growth of these fungi. Anti-dandruff shampoo contains antifungal active compounds such as ZnPtO, selenium sulfide, piroctone olamine which functions in reducing fungal infections on the scalp which are expected to inhibit the growth of fungi found on the scalp that cause dandruff. This study aims to determine the inhibition of the active compound in anti-dandruff shampoo against fungal growth isolated from dandruff. This research is a laboratory experimental study which was carried out by isolating the fungus from dandruff and continued by testing the inhibition of fungal growth using a shampoo containing active compounds. From the results of research conducted on 3 shampoos that have different anti-dandruff compounds, namely ZnPtO, 1% selenium sulfide, and piroctone olamine, the growth of fungal colonies decreased with different contact times, namely 2 minutes, 4 minutes, and 6 minutes. The conclusion of the study stated that shampoo containing piroctone olamine compound with a contact time of 6 minutes inhibited the growth of fungal colonies better than other shampoos containing 1% selenium sulfide and zinc pyrithione (ZnPtO).
Gambaran Kadar Kolesterol Total pada Pengguna Rokok Elektrik Arisanti, Dewi; Basarang, Mujahidah; Rianto, Muh. Rifo; Rustiah, Waode; Bano, Siti Maryam
Lontara Journal of Health Science and Technology Vol. 4 No. 2 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v4i2.404

Abstract

An electric cigarette is a tool that functions to convert chemical substances into the form of vapor and distributes them to the lungs using electricity. One of the harmful elements contained in cigarettes is nicotine. If nicotine circulates in the body, it stimulates the secretion of the adrenaline hormone resulting in an increase in heart rate, blood pressure and changes in fat metabolism. This study aims to describe cholesterol levels in e-cigarette users in Mamajang sub-district. This research is a laboratory observation which aims to describe cholesterol levels in e-cigarette users in Mamajang district. The number of samples used were 20 samples. The object of this research is the blood of electric smokers using the POCT method. From the results of research conducted on 20 samples. 6 of them got increased results and 14 samples got normal. So it can be concluded that of the 20 samples, 6 samples (74%) got normal results and 6 samples (26%) got increased results.
EKSTRAK DAUN JATI MUDA UNTUK PEWARNA PEMERIKSAAN SEDIMEN URINE Setiawan, Doni; Farihatun, Atun; Nurmalasari, Ary; Tia Istianah, Euis; Basarang, Mujahidah; TS Maulidiyanti2, Ellies
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20647

Abstract

Urinalysis examination is needed to find information and determine the condition of the kidneys, urinary tract or other organs such as the liver, pancreas, and bile ducts. A urinary sediment examination aims to see organic and inorganic elements in the urine by preparing using Sternheimer Malbin stain reagents. Sternheimer Malbin stain has the disadvantage that it is a synthetic chemical. Stain from natural ingredients becomes a solution as an alternative to reduce the use of synthetic chemicals. Natural stains with the same characteristics and widely tested for bacterial and fungal stains are natural stains from teak leaf extract. Young leaf extract contains anthocyanins in red colour. This study aimed to determine the ability of ethanol extract from young leaves as a natural stain for urine sediment examination. The research method is an experiment with the Static Group Comparison research design. The population and samples in this study are teak leaves determined at the Galuh University Laboratory of the Biology Study Program with the Latin name (Tectona grandis L.f.). The results of the Fisher Exact test obtained a p (value) <0.050, so there was no significant difference between sediments without stain, Sternheimer Malbin, and ethanol extract of young leaves in colouring urine sediments. The conclusion is that the dye of ethanol extract from young teak leaves effectively colours the components of urine sediment, namely epithelial cells, leukocyte cells, and erythrocyte cells with red colour. Keywords      : Anthocyanins, Alternative Dyes, Sternheimer malbin