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All Journal ACTA VETERINARIA INDONESIANA Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary
Hana Eliyani
Department Of Veterinary Anatomy, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Effect of Aflatoxin Contaminated Feed for 40 Days on Anatomy and Histopathology of the Thymus of Laying Hens in the Starter Phase Christy, Rachella; Soeharsono, Soeharsono; Ismudiono, Ismudiono; Eliyani, Hana; Sarmanu, Sarmanu; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28679

Abstract

The purpose of this study was to determine the effect of aflatoxin contaminated feed given for 40 days on the anatomy and histopathology of the thymus of laying hens in the starter phase. A total of 36 DOC laying hens were divided into two groups (P0 and P1). P0 (control group) was given 100% basal starter feed CP 521 and P1 (treatment group) was given 80% basal feed CP 524 plus 20% aflatoxin contaminated feed. Feeding contaminated with aflatoxin was carried out for 40 days, starting on the 20th to the 60th day after the DOC of laying hens was adapted for 20 days. The time of surgery in this study was carried out on the 20th, 40th, and 60th days, where at each surgery time the thymus organ was taken to collect weight data and histopathological preparations were made. The thymus weights based on time showed a significant decrease, but the intergroup thymus weights did not show a significant decrease. The decrease in thymus weight is caused by the involution process. Microscopic observations based on increased cell debris and congestion showed that P0 did not show a significant difference, but P1 showed a significant difference (p>0.05) on the 40th and 60th days. The conclusion in this study was that as much as 20% of feed contaminated with aflatoxins had not affected changes in thymus weight but had shown damage to the histopathological structure of the thymus.
Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Anugrah, Alvyan Lantang; Eliyani, Hana; Utomo, Budi; Susilowati, Suherni; Mafruchati, Maslichah; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
KORELASI ANTARA LINGKAR DADA, PANJANG BADAN DAN TINGGI GUMBA TERHADAP VOLUME SEMEN DAN KONSENTRASI SPERMATOZOA SAPI MADURA Mayola, Dilla Aranda; Rimayanti, Rimayanti; Warsito, Sunaryo Hadi; Utama, Suzanita; Eliyani, Hana; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.57 KB) | DOI: 10.20473/ovz.v8i2.2019.105-109

Abstract

The aim of this research was to know the correlation between heart girth, body length, and wither's height with semen volume and sperm concentration of Madura Bulls. Ten Madura bulls aged three to four years from Madura  were measured. Variables observed were heart girth, body length, wither's height, semen volume and sperm concentration. Data were analyzed by multiple linear regression analysis. The result of this research showed that there was no correlation between heart girth, body length, and wither's height with semen volume Y = 25,212 + 0,190 X1+ (-0,131) X2 + 0,136 X3, so as there was no correlation between heart girth, body length and wither's height with sperm concentration Y = 8739,559 + (-36,663) X1+ (19,714) X2 + 6,2960 X3. And than was no correlation between semen volume and sperm concentration Y = 7,303 + (-0,002) X1.
HUBUNGAN MORFOMETRI AMBING TERHADAP PRODUKSI SUSU KUDA DI DAERAH BIMA NUSA TENGGARA BARAT Mujahid, Mujahid; Mustofa, Imam; Tehupuring, Benjamin Chr; Restiadi, Tjuk Imam; Eliyani, Hana; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.647 KB) | DOI: 10.20473/ovz.v8i2.2019.169-174

Abstract

Objective from this research was to determine the relationship of udder morphometry which consists of the height of the front udder, the height of the rear udder and the circumference ofudder on milk production in the Bima District of West Nusa Tenggara. One of ways to choose a good lactational mare is to pay attention at the shape of the part external body which is udder. The sample had used 32 mares that were in lactation period. Milk production has been measured from the average milk production per day in the lactation period. Milking process in Bima District, West Nusa Tenggara has done in three to four times a day in the span of four to five hours per day. Body length had been measured from os thoracalis to os coxae, chest circumference had been measured from os vertebrae thoracalis to os thoracalis, posterior abdomen of pelvic to os pelvis and the last of os lumbar vetebrae. The research design in this study was survey on every wild mares rancher. Data that has been collected and processed using Multiple Regression with SPSS program version 23th. The result showed that there was a strong relationship between height of rear udder and udder circumference to milk production with back udder with height coefficient of 0.21 (P <0.05) and udder circumference was 0.11 (P <0.05) and equation in Y = 2.423 - 0.398LA + 0.793TAB. However, there is no significant relationship between the height of the front udder and milk production.
The effect of red dragon fruit skin extract (hylocereus polyrhizus) on the number of leydig cells, diameter of seminiferous tubules, and testicular weight of male mice (mus musculus) exposured with hot temperatures Prastyaningtyas, Kukuh; Kurnijasanti, Rochmah; Sugihartuti, Rahmi; Susilowati, Suherni; Suprayogi, Tri Wahyu; Eliyani, Hana
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.18-24

Abstract

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.
Efek pemberian α-tokoferol terhadap jumlah sel spermatogenik dan sel Leydig pada tikus putih (Rattus norvegicus) yang dipapar 2,3,7,8 Tetrachlorodibenzo-p-dioxin Machmudia, Ainun; Eliyani, Hana; Widjiati, Widjiati; Wurlina, Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.72-80

Abstract

 This research aimed to determine the effect of α-tocopherol on the count of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (dioxin). Dioxin is an endocrine-disrupting agent that adversely affects reproductive health, while α-tocopherol maintains fertility. This research used 25 rats aged 10-12 weeks weighing 150-200 grams. Rats were divided into five groups (K, P0, P1, P2 and P3). The K (control group) was administered with corn oil 1 ml/day. P0 was exposed to 700 ng/kg/day dioxin. P1, P2 and P3 was exposed to dioxin at a dose of 700 ng/kg/day and administered with α-tocopherol at a dose of 77, 140 and 259 mg/kg/day respectively. Dioxin exposure, corn oil and α-tocopherol administration were conducted orally for 20 days. On day-21, all rats were sacrificed for histological slides preparation of testicles with hematoxylin-eosin staining. Data were analyzed with analysis of variance and continued with the Duncan test. The results indicated that exposure to dioxin caused a decrease in the number of spermatogenic and Leydig cells. The administration of α-tocopherol at a dose of 140 mg/kg/day eliminated the effect of reducing the number of spermatogenic and Leydig cells caused by exposure to dioxin. The conclusion was the administration of α-tocopherol at 140 mg/kg/day was effective in maintaining the number of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin.
Co-Authors Adikara, Tatang Santanu Anton Sulistiono Anugrah, Alvyan Lantang Benjamin C Tehupuring Berlina, Cyrcilia Relita Budi Utomo Cahyono, Shahani Azpriyanne Chairul Anwar Chrismanto, Dony Christy, Rachella Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Hani Plumeriastuti Hendarti, Gracia Angelina Hermin Ratnani Hidajati, Nove IMAM MUSTOFA Indah Norma Triana, Indah Norma Ismudiono Ismudiono, Ismudiono Jihan Pangestuningrum Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Machmudia, Ainun Mafruchati, Maslichah Mayola, Dilla Aranda Mufasirin Muhammad Ahdi Kurniawan Mujahid Mujahid, Mujahid Nunuk Dyah Retno Lastuti Poedji Hastutiek Poetranto, Emmanuel Djoko Prastyaningtyas, Kukuh Pratama, Dio Putra Rahmi Sugihartuti, Rahmi Ratna Damayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rochmi, Siti Eliana Sami, Abdul Santoso, Kuncoro Puguh Sarmanu, Sarmanu Soeharsono Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Suzanita Utama Talita Yuanda Reksa Tehupuring, Benjamin Chr Tehupuring, Benjamin Chr. Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahidan Qodiip Maulana Warsito, Sunaryo Hadi Wenny Nur Azizah Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wurlina, W