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Produksi Konsentrat Protein dari Ampas Kelapa (Cocos nucifera L.) Menggunakan NaOH dan (NH4)2SO4 : Production of Protein Concentrate from Coconut Pulp (Cocos nucifera L.) using NaOH and (NH4)2SO4 Hasdinda, Hasdinda; Satrimafitrah, Pasjan; Bahri, Syaiful; Razak, Abd. Rahman; Puspitasari , Dwi Juli; Indriani; Amar, Aini Auliana
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 1 (2023): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i1.16290

Abstract

Coconut pulp wasted from processed coconut oil has a protein content of up to 18.20%. The potential coconut pulp protein can be processed in protein concentrate which has many benefits. This research was conducted to determine the effect of NaOH and (NH4)2SO4 concentrations in isolating protein and producing protein concentrates that have the highest yield and protein content. Isolation of coconut pulp protein was carried out using NaOH at several concentrations, namely 0, 0.2, 0.4, 0.6, 0.8, and 1 M, while the protein concentrate was prepared using the salting out method with a saturation level of the ammonium sulfate salt of 55%, 60%, and 65%. Analysis of protein content was carried out using the Kjeldahl method. The use of NaOH 0.2 M and (NH4)2SO4 with a saturation level of 60% was the chosen treatment with crude protein content and yield of 75.63% and 33.83%, respectively.
Penentuan Kadar Albumin Ikan Gabus (Channa striata) dengan Metode Biuret setelah Dikukus dan Dipepes: Determination of Albumin Content of Snakehead Fish (Channa striata) by Biuret Method after Steamed and Wrapped Naser, Inda Hastuti N; Bahri, Syaiful; Satrimafitrah, Pasjan; Razak, Abd. Rahman; Ruslan; Ridhay, Ahmad; Puspitasari, Dwi Juli; Khairuddin
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 2 (2023): August Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i2.16391

Abstract

Snakehead fish (Channa striata) is a type of freshwater fish that contains high levels of protein albumin. The research was conducted to determine the albumin content in snakehead fish after the cooking process, both steamed and wrapped. The research was conducted using a completely randomized design with a factorial pattern, the first factor was the cooking method (steamed and wrapped) and the second was cooking time (10, 15, 20, and 20 minutes). Determination of protein albumin levels in snakehead fish after processing using the Biuret method. The steaming process for 15 minutes resulted in protein content in the snakehead fish of 0.76%, while the protein albumin that was lost was 19.28%. The wrapped method for 20 minutes has a remaining protein content of 0.59%, while the missing protein is 38.78%. The method of processing snakehead fish by steaming is better than the wrapping process.