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Asosiasi Glomus sp. dan Gigaspora margarita pada bibit Aquilaria malaccensis Endah SUSILOWATI; Melya RINIARTI; Maria Viva RINI
Menara Perkebunan Vol. 87 No. 2 (2019): 87 (2), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i2.342

Abstract

AbstractAgarwood (Aquilaria malaccensis) is one of the non-timber forest products that has high economic value. However, this plant grows very slowly.  Therefore, seed technology input is needed, such as addition of arbuscular mycorrhizal fungi (AMF). The objectives of this research were to study the effect of AMF inoculum i.e. Glomus sp., Gigaspora margarita and mixture of both species on agarwood growth and to determine the best AMF type for agarwood seedlings growth. The experimental research design used was a completely randomized design with three treatments, namely Glomus sp. (G), G. margarita (Gi) and a mixture of Glomus sp. and G. margarita (GGi) and without AMF inoculation (K) with each treatment repeated 8 times. The number of AMF spores used was ± 300 spores/seedling, and applied at the time of transplanting from germination tray to the polybag. Data were processed using analysis of variance and least significant difference test (LSD). The results showed there was AMF colonization in agarwood roots after 12 weeks of inoculation with the highest percentage at 20.50% on G. margarita treatment. Overall, AMF improved the growth of agarwood seedlings. The best seedlings growth was in the treatment of mixed AMF (GGi). Increased growth was found in plant height (14.68 cm), seedling diameter (2.16 mm), leaf area (119.30 cm2), root volume (1.15 mL), total dry weight (0.83 g), and shoot root ratio (4.99).[Keywords: agarwood, A. malaccensis,AMF, G. margarita,Glomussp.]AbstrakGaharu (Aquilariamalaccensis) adalah salah satu hasil hutan bukan kayu yang memiliki nilai ekonomis tinggi. Namun tanaman ini memiliki pertumbuhan yang lambat, sehingga diperlukan input teknologi pembibitan berupa penambahan fungi mikoriza arbuskular (FMA). Penelitian bertujuan untuk mengetahui pengaruh pemberian FMA tunggal Glomussp., Gigaspora margaritadan campuran keduanya (Glomussp. dan G. margarita)terhadap pertumbuhan gaharu serta menentukan isolatyang menghasilkan pertumbuhan bibit gaharu yang lebih baik.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap untuk menguji tiga perlakuan yaitu Glomussp. (G), G. margarita (Gi) dan campuran Glomus sp. dan G. margarita(GGi) serta satu kontrol (K) dengan  masing – masing perlakuan diulang 8 kali. Inokulum FMA yang digunakan memiliki kepadatan ±300 spora/bibit dan diinokulasikan saat bibit dipindahkan dari persemaian ke polybag. Data diolah menggunakan analisis varians dan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa ditemukan adanya kolonisasi FMA dalam akar gaharu setelah 12 minggu inokulasi dengan persentase kolonisasi tertinggi 20,50% oleh G. margarita.  Secara keseluruhan, pemberian FMA mampu meningkatkan pertumbuhan bibit gaharu. Pertumbuhan terbaik terjadi pada perlakuan FMA campuran (GGi).  Peningkatan pertumbuhan secara nyata dapat dilihat pada tinggi tanaman (14,68 cm), diameter bibit (2,16 mm), luas daun (119,30 cm2), volume akar (1,15 mL), bobot kering total (0,83 g) dan nisbah tajuk akar (4,99).[Kata kunci: A. malaccensis, FMA, gaharu, G. margarita, Glomus sp.]
Selection of four types arbuscular mycorrhizal fungi in oil palm seedling planted in histosol soil Maria Viva RINI; Radix SUHARJO; Lestari WIBOWO; David IRVANTO; Adhy ARIYANTO
Menara Perkebunan Vol. 89 No. 1 (2021): 89 (1), 2021
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.406

Abstract

Arbuscular mycorrhizal fungi (AMF) are beneficial microbes for plants, especially in the absorption of nutrients and water from the soil. Some factors that influence the effectiveness of this fungus are the suitability of the AMF species with their host plants and abiotic condition such as soil characteristics. Therefore, this study aims to determine the type of AMF that produced the best growth and nutrient uptake in oil palm seedlings planting in histosol soil. This study used a single factor treatment design consisting of 9 AMF treatments, namely control without AMF (T1), given Glomus sp. (T2), Gigaspora sp. (T3), Entrophospora sp. (T4), Acaulospora sp. (T5), a mixture of Glomus sp. and Gigaspora sp. (T6), a mixture of Glomus sp. and Entrophospora sp. (T7), a mixture of Gigaspora sp. and Entrophospora sp. (T8), and a mixture of Glomus sp., Gigaspora sp., Entrophospora sp., and Acaulospora sp. (T9). Each treatment was repeated five times. The experimental design used was a randomized block design. In the AMF treatment, 500 spores per seedling were given when the seedlings were transplanted from prenursery to the main nursery. The seedlings were kept in the prenursery for 3.5 months and in the main nursery for nine months. The results showed that the type of mycorrhizal that consistently produced better seedling growth and nutrients uptake compared to the control were Glomus sp. (T2) and a mixture of Gigaspora sp. and Entrophospora sp. (T8), which supported by data on plant height, number of leaves, shoot dry weight, and total nutrient uptake of N, P, K, Ca, Mg, and B.
Pengaruh Volume Media Tanam dan Jumlah Tanaman Inang pada Produksi Spora Fungi Mikoriza Arbuskular Rini, Maria Viva; Santoso, Ramadian Budi; Sugiatno, Sugiatno
JURNAL AGROTROPIKA Vol. 22 No. 1 (2023): Jurnal Agrotropika Vol 22 No 1, Mei 2023
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v22i1.6696

Abstract

Produksi fungi mikoriza arbuskular sangat dipengaruhi oleh media tanam dan tanaman inang. Oleh karena itu penelitian ini bertujuan untuk mendapatkan volume media tanam dan jumlah tanaman inang yang terbaik untuk memproduksi spora fungi mikoriza arbuskular (FMA). Penelitian menggunakan rancangan faktorial 5x 5 dengan faktor pertama volume media tanam yang terdiri dari 200 ml, 400 ml, 600 ml, 800 ml, dan 1000 ml. Sedangkan faktor ke dua adalah jumlah tanaman inang per pot yang terdiri dari 1, 2, 3, 4, dan 5 tanaman. Setiap perlakuan diulang sebanyak 4 kali. Media tanam yang digunakan adalah campuran pasir sungai steril dengan zeolite dengan perbandingan 1: 1 berdasarkan volume dan tanaman inang yang digunakan adalah Pueraria javanica. Tanaman inang dipelihara di rumah kaca hingga 4 bulan kemudian dikeringkan (tanpa disiram selama 2 minggu) untuk memicu produksi spora. Data hasil penelitian menunjukkan bahwa volume media tanam yang paling sesuai untuk memproduksi spora FMA adalah 400 ml dengan jumlah tanaman inang sebanyak 5 tanaman. Dari perlakuan ini diperoleh jumlah spora terbanyak dibandingkan perlakuan lainnya.
Populasi dan Keanekaragaman Fungi Mikoriza Arbuskular pada Rizosfir Tanaman Lada Monokultur dan Polikultur Sari, Oktafia; Rini, Maria Viva; Evizal, Rusdi; Karyanto, Agus
JURNAL AGROTROPIKA Vol. 23 No. 1 (2024): Jurnal Agrotropika Vol 23 No 1, Mei 2024
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v23i1.8593

Abstract

Arbuscular mycorrhizal fungi (AMF) is a type of fungus that comes from the endomycorrhizal group, these fungi have the ability to form symbiosis with almost 90% of higher plant species. Population and diversity of AMF are influenced by biotic factors and abiotic factors. This research aims to determine the differences in population, diversity and dominant types of AMF in the rhizosphere of pepper planted in monoculture and pepper in polyculture. The soil samples were taking from monoculture and polyculture pepper plantations in Air Naningan District, Tanggamus Regency. The AMF population was tested using the One way Annova test. Culture trapping was done using a factorial treatment with the first factor being the origin of the soil sample (K) and the second actor being the type of host plant (T). Treatments design used was randomize design (CRD). The results showed that the AMF population in polyculture pepper plantation soil samples was higher than monoculture pepper, based on the Shannon-Wiener Diversity Index, AMF diversity in monoculture pepper and coffee intercropping pepper plantations was higher than in cocoa intercropping pepper plantations, and the type of AMF was dominant. from the results of trapping culture with monoculture pepper garden soil samples, namely spores of the species code S7, in samples of coffee intercropping pepper plantations the code S7 is dominant, while in cocoa intercropping pepper plantations it is dominated by code S6. Keywords: Arbuscular mycorrhizal fungi, diversity, population.
PENGARUH DOSIS DAN INTERVAL PUPUK DASAR TERHADAP PERTUMBUHAN TANAMAN NANAS (Ananas comosus [L.] Merr.) Aulia, Fathia Nur; Rini, Maria Viva; Widyastuti, R.A. Diana; Karyanto, Agus
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9925

Abstract

Pineapple (Ananas comosus [L.] Merr.) is one type of fruit that has an important role in Indonesia because it is a major export commodity, both in fresh and processed forms such as canned fruit and juice. Therefore, the development of pineapple production is a strategic matter. One of the steps taken to increase pineapple production is through proper and balanced fertilization. The objective of this study was to determine the best fertilizer package that supports the growth of pineapple plants. The research was conducted from November 2022 to March 2023 using a single-factor Randomized Group Design (RAK), consisting of treatments and repeated 4 times.  The treatment packages tested included the dose and interval of fertilization, namely: P0= 200 kg DAP + 200 kg Kiesirite + 300 kg K2SO4 + 50 kg ZA per ha (0, 30 HST), P1= 300 kg DAP + 300 kg Kiesirite + 300 kg K2SO4 + 50 kg ZA per ha (0, 30, 60 HST), P2= 300 kg DAP + 300 kg Kiesirite + 300 kg K2SO4+ 50 kg ZA per ha (0, 15, 45 HST), P3= 350 kg DAP + 350 kg Kiesirite + 350 kg K2SO4 + 50 kg ZA per ha (0, 30, 60 HST), and P4= 350 kg DAP + 350 kg Kiesirite + 350 kg K2SO4 + 100 kg ZA per ha (0, 15, 45 HST). In each experimental unit, 5 sample plants were selected, so that the total plants observed were 100 sample plants. The data were analyzed using analysis of variance, and the F test showed significant differences which were then further tested with the Least Significant Difference (BNT) test at the 5% level.  The results showed that in general, fertilizer doses and intervals had no significant effect on most of the observed variables, except on d-leaf width and d-leaf length.  The fertilizer package in the P0 treatment (200 kg DAP + 200 kg Kiesirite + 300 kg K2SO4 + 50 kg ZA) per ha proved to be the best to support pineapple plant growth. Keywords: dosage, fertilization frequency, pineapple, inorganic fertilizer
Population and Diversity of Arbuscular Mycorrhiza Fungi in the Rhizosphere of Kasetsart Cassava Clone Grown on Two Different Locations Rini, Maria Viva; Sitio, Selly Novita Sari; Hidayat, Kuswanta Futas
JOURNAL OF TROPICAL SOILS Vol. 22 No. 3: September 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i3.183-189

Abstract

Population and diversity of arbuscular mycorrhiza fungi (AMF) are varied in the soil and influenced by biotic factors such as host plant and abiotic factors such as soil fertility, soil moisture, pH, temperature, etc.  This study aimed to determine the population, diversity, and the dominant type of AMF in the rhizosphere of Kasetsart cassava clones obtained from LampungTimur and Tulang Bawang Barat Regencies, Lampung Province, Indonesia. Population of AMF was counted directly from the rhizosphere of Kasetsart cassava clones and the diversity of AMF was assesed using a pot culture experiment.  The results showed that the population and the diversity of AMF in the rhizosphere of Kasetsart clone obtained from Tulang Bawang Barat was higher than that from LampungTimur.  The predominant type of AMF found in the pot culture using soil samples from Lampung Timur was spore with S2 code that belongs to the genus Gigaspora and S4 code that belongs to the genus Glomus. On the other hand, the type of AMF found in the rhizosphere of soil samples from Tulang Bawang Barat was dominated by spore with S9 code that belongs to the genus Entrophospora.
The Growth of Oil Palm (Elaeis guineensis Jacq.) Seedlings with the Application of Different Arbuscular Mycorrhiza Fungi and Various Phosphorous Dosages Krisnarini, Krisnarini; Rini, Maria Viva; Timotiwu, Paul Benyamin
JOURNAL OF TROPICAL SOILS Vol. 23 No. 3: September 2018
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2018.v23i3.117-124

Abstract

The effectiveness of Arbuscular Mycorrhiza Fungi (AMF) colonization depends on the type of AMF, plant species and phosphorus fertilization.  The aims of this study were to determine: the best type of AMF and the appropriate dosage of P fertilizer for the growth of oil palm seedlings, and whether the oil palm seedling responses to the application of AMF type is determined by the dosage of phosphorus applied. The experiment was arranged in a Randomized Complete Block Design (RCBD) with two factors and 5 replications. The first factor was the types of AMF (m), consisting of without mycorrhiza inoculation (m0), Gigaspora sp. MV16 isolate (m1), Glomus sp. MV7 isolate (m2), Gigaspora sp. MV16 isolate + Glomus sp. MV7 (m3) isolate.  The second factor was phosphorus fertilization (SP-36), consisting of 3 levels: 1/3 recommended dosage (p1), 2/3 recommended dosage (p2), full recommended dosage (p3). The results showed that the application of all AMF types resulted in the same effects on the growth of oil palm seedlings, however the seedling growth in the inoculated plants were better than in uninoculated plants (control). The application of the recommended dosage of P fertilizer produced the highest seedling growth. The response of seedlings to the application of AMF type was not determined by doses of phosphorus fertilizer applied.
PENGARUH APLIKASI TIGA JENIS BIOCHAR DAN PUPUK FOSFOR TERHADAP KEBERADAAN FUNGI MIKORIZA ARBUSKULAR SERTA PERTUMBUHAN TANAMAN JAGUNG (Zea mays) DI LTPD UNILA Sholihah, Fairuz Nabila; Yusnaini, Sri; Rini, Maria Viva; Arif, M. A. Syamsul
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.10644

Abstract

Fungi Mikoriza Arbuskular (MA) merupakan salah satu fungi yang melakukan simbiosis mutualisme dengan akar tanaman dan memberikan beberapa manfaat.Penelitian ini bertujuan untuk mengetahui pengaruh pemberian biochar terhadap keberadaan fungi MA, mengetahui pengaruh pemberian pupuk fosfor terhadap keberadaan fungi MA pada pertanaman jagung, serta mengetahui interaksi antara jenis biochar dengan dosis pupuk fosfor terhadap keberadaan fungi MA pada pertanaman jagung.  Penelitian ini dilaksanakan pada Maret hingga Oktober 2022 di Laboratorium Lapang Terpadu (LTPD) dan Laboratorium Produksi Perkebunan dan Mikoriza Fakultas Pertanian Universitas Lampung.  Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) faktorial dengan dua faktor.  Faktor pertama biochar (B) terdiri dari tanpa biochar (B0), biochar sekam padi (B1), biochar tongkol jagung (B2), dan biochar batang singkong (B3), faktor kedua pupuk fosfor (P) terdiri dari tanpa pupuk fosfor 0 kg P2O5 ha-1 (P0) dan dengan pupuk fosfor 100 kg P2O5 ha-1 (P1).  Hasil penelitian menunjukkan bahwa pemberian biochar tidak berpengaruh nyata terhadap populasi fungi MA maupun persen kolonisasi akar oleh fungi MA.  Pemberian pupuk fosfor tidak berpengaruh nyata terhadap populasi fungi MA maupun persen kolonisasi akar oleh fungi MA.  Terdapat interaksi antara kedua perlakuan terhadap bobot brangkasan kering panen, bobot biji kering, dan populasi fungi MA.  Hasil analisis menunjukkan bahwa interaksi biochar sekam padi tanpa pupuk fosfor meningkatkan populasi fungi MA paling tinggi dibandingkan interaksi lainnya.
Co-Authors Adhy ARIYANTO Agus Karyanto Agus Karyanto Ainin Niswati Ananda Y. Rahmayanti Arif, M. A. Syamsul Aulia, Fathia Nur Azzahra, Salwa Catur Putra Satgada Catur Putra Satgada, Catur Putra Citra Bara Kurniastuty Dad Resiworo J. Sembodo David IRVANTO Decha Bagus Saputra Dermiyati Dermiyati Eldineri Zulkarnain Eldineri Zulkarnain, Eldineri Endah SUSILOWATI Fitri Yelli Fluenty Dwitama Hanum Riajeng Amalia Hanum Riajeng Amalia, Hanum Riajeng Hidayat Pujisiswanto Hidayat Saputra Hindun Nur Haqiqie Husna Fii Karisma Jannah Husna Husna Ina Febria Ginting Inggar Damayanti Jamalam Lumbanraja Jamalam Lumbanraja Krisnarini, Krisnarini Kus Hendarto Kusuma Oka Pertiwi Kuswanta Futas Hidayat Kuswanta Futas Hidayat Kuswanta Futas Hidayat Kuswanta Futas Hidayat Lestari Wibowo Listyowati, Mastutik Sri Lita Andriyyani M. A. Syamsul Arif M. A. Syamsul Arif Mas Achmad Syamsul Arif Maulana Malik Melya Riniarti Minarsih Minarsih Muhamad Gary Ranchiano Novalim Purlasyanko Novri Dwi Damayanti Novri Dwi Damayanti Palasta, Rio Paul Benyamin Timotiwu Puput Azizah Raden Ajeng Diana Widyastuti Radix Suharjo Ramadian Budi Santoso Ramires, Ryano Retta Ramadhina Rias Retta Ramadhina Rias Rio Palasta Rugayah Rusdi Evizal Sanjaya, Purba Santoso, Ramadian Budi Sari, Oktafia Sarno Sarno Septiana, Liska Mutiara Sholihah, Fairuz Nabila Silfi Indrasari Sitio, Selly Novita Sari Sri Yusnaini Sri Yusnaini Sri Yusnaini Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Sugiatno Syaifudin Nur Hasan Tegar Rafshodi Awang Tegar Rafshodi Awang, Tegar Rafshodi Titiek Nur Aeny Usnaqul EFRIYANI Vida Rozalinda Wiwik Agustina Wiwik Agustina, Wiwik Yunita Siwi Palupi