Article Per Year (5 Year)
p-Index From 2020 - 2025
7.752
P-Index
This Author published in this journals
All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia Jurnal Pendidikan Sains AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas
Oktira Roka Aji
Program Studi Biologi, FAST, Universitas Ahmad Dahlan
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

Published : 57 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 57 Documents
Search

 1   2   3   4   5 
Expression and Purification of PhoR Sensor-Domain Histidine Kinase of Mycobacterium tuberculosis in Escherichia coli ERNAWATI ARIFIN GIRI-RACHMAN; FENRYCO PRATAMA; OKTIRA ROKA AJI; ARUM PATRIATI; IHSANAWATI IHSANAWATI; MAELITA RAMDANI MOEIS; EDY GIRI-RACHMAN PUTRA
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1382.183 KB) | DOI: 10.5454/mi.9.2.1

Abstract

Globally, tuberculosis (TB) remains a leading cause of death. The emergence of multidrug-resistant strains (MDR-TB) and extensively drug-resistant strains (XDR-TB) has fuelled the discovery for novel drugs and drug targets for its successful and better treatment. One of the potential candidates for drug target is PhoR sensory protein histidine kinase, a part of the Two Component System (TCS) PhoP/PhoR in Mycobacterium tuberculosis (Mtb). This protein system was known for its role on regulating hundred of Mtb virulence factors, from genes for cell wall and lypid synthesis to genes for adaptation in human leukocyte and hypoxia response. Previous studies have successfully characterized, isolated, and cloned the putative sensory domain of PhoR protein gene into pRSET vector expression system. In this study, Escherichia coli was transformed with pRSET-SensPhoR and cultivated at 37oC under IPTG induction to express PhoR sensor-domain protein. Most of the proteins were overexpressed in the form of inclusion bodies.  Subsequent protein purification in Ni-NTA system under refolding condition on urea gradient was performed to isolate PhoR sensor-domain protein in soluble form. Arginine was supplemented in purified protein solution to prevent aggregation during long term storage.  While highly purified protein was acquired, small angle X-ray scattering (SAXS) analysis was conducted to obtain 3-dimensional (3D) protein structures in solution.    doi:10.5454/mi.9.2.1 
Pengaruh Ekstrak Daun Jeruju (Acanthus ilicifolius L.) dalam Menghambat Trichodina spp. pada Benih Ikan Nila (Oreochromis niloticus L.) Andika Bayu Chandra; Oktira Roka Aji
JRST (Jurnal Riset Sains dan Teknologi) Volume 7 No. 2 September 2023: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v7i2.16749

Abstract

Ikan Nila (Oreochromis niloticus L.) adalah ikan yang digemari oleh masyarakat luas. Namun, dalam pembudidayaannya tidak terlepas dari berbagai masalah penyakit, khususnya yang disebabkan oleh ektoparasit. Trichodina spp. merupakan salah satu jenis ektoparasit yang seringkali menyerang benih ikan Nila. Pengendalian Trichodina spp. dapat dilakukan dengan penggunaan bahan alam yang ramah lingkungan. Salah satu bahan alam yang berpotensi digunakan untuk mengurangi intensitas serangan Trichodina spp. adalah daun Jeruju (Acanthus ilicifolius L.). Penelitian ini bertujuan untuk memanfaatkan daun Jeruju sebagai agen untuk menghambat ektoparasit Trichodina spp. pada ikan Nila. Pengujian dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dengan dosis yang berbeda yaitu 0 g/L; 0,25 g/L; 0,5 g/L; 1 g/L dan 2 g/L. dan pengulangan 5 kali. Ikan Nila direndam pada dekokta daun Jeruju selama 24 jam lalu diamati prevalensi dan intensitas serangan Trichodina spp. serta kelangsungan hidup benih ikan nila. Hasil penelitian menunjukan perlakuan terbaik yaitu perendaman dengan dosis 2 g/L yang memiliki prevalensi serangan Trichodina spp. sebesar 53,33%, dengan intensitas Trichodina spp. sebesar 1,34 pada kulit dan 0,734 pada insang serta kelangsungan hidup benih ikan nila yaitu 100%. Berdasarkan penelitian dapat disimpulkan bahwa pemberian dekokta daun Jeruju dengan berbagai dosis berpengaruh nyata terhadap prevalensi dan intensitas Trichodina spp. serta kelangsungan hidup benih ikan nila.
Antibacterial Efficacy of Turmeric (Curcuma domestica) Rhizome Infusion Against Aeromonas hydrophila and Its Toxicity Nur Azizah; Oktira Roka Aji
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.16934

Abstract

Aeromonas hydrophila bacteria can cause Motile Aeromonas Septicemia (MAS), a disease that impacts freshwater fish. The turmeric rhizome contains numerous bioactive compounds that act as antibacterials and might be utilized to inhibit A. hydrophila. This research aimed to identify the phytochemicals in turmeric (Curcuma domestica) infusion, evaluate its antibacterial activity against A. hydrophila, and define the toxicity effect of turmeric rhizome infusion. The agar dilution method was used to measure antibacterial activity, whereas the Brine Shrimp Lethality Test (BSLT) was used to assess toxicity. The research confirmed the presence of phytochemicals such as tannins, alkaloids, flavonoids, phenols, and saponins in a turmeric rhizome infusion. According to the findings, the infusion from turmeric rhizome effectively inhibited the growth of A. hydrophila at concentrations between 750-1000 ppm. In toxicity tests, the LC50 value of the turmeric rhizome infusion against shrimp larvae was 381.18 ppm. Hence, the turmeric rhizome infusion has the potential to be further developed for the prevention and treatment of fish infected with A. hydrophila in freshwater fish farming.
Analysis of Genetic Engineering Material Misconceptions in Biology Textbooks for Ninth-Grade in Yogyakarta Silfi Pratiwi; Purwanti Pratiwi Purbosari; Oktira Roka Aji; Rinaldi Rizal Putra
Jurnal Pendidikan Sains Vol 12, No 2: June 2024
Publisher : Pascasarjana Universitas Negeri Malang (UM)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Textbooks play a pivotal role in the pedagogical landscape, serving as indispensable aids for learning, founts of knowledge, and platforms for practical application. This study endeavors to scrutinize the content of textbooks, specifically focusing on ninth-grade biology textbooks within the educational milieu of Yogyakarta City, and to juxtapose these contents with existing literature to discern potential misconceptions. Employing a descriptive qualitative approach, the investigation centers on printed biology textbooks utilized in ninth-grade curricula across junior high schools in Yogyakarta. The selection of textbooks for analysis was based on their prevalence as instructional materials, as determined through survey data. Genetic engineering emerged as the focal point for identifying misconceptions within the chosen textbooks. The tool employed for this purpose was a meticulously crafted misconception analysis sheet, utilized alongside textual scrutiny. Analysis revealed the presence of misconceptions within both Textbooks A and B. Textbook A exhibited one instance of misidentification and one of oversimplification, while Textbook B featured two instances of oversimplification. The prevalence of misconception categories in Textbook A was calculated at 5.88% for misidentification and an equal percentage for oversimplification. In Textbook B, the percentage rose to 15.38%, exclusively attributed to oversimplification. Despite the relatively low incidence of misconceptions in both textbooks, these findings underscore the imperative of mitigating such inaccuracies in future editions.DOI: 10.17977/jps.v12i22024p063
Pelatihan Pembuatan Mikroorganisme Lokal Untuk Pupuk Organik Di Desa Banaran, Kulon Progo Oktira Roka Aji; Ambar Pratiwi
Jurnal Pengabdian Masyarakat Indonesia Vol 4 No 4 (2024): JPMI - Agustus 2024
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.2769

Abstract

Ketergantungan petani pada pupuk kimia sintetis telah menyebabkan kerusakan struktur tanah dan penurunan kesuburan, yang pada akhirnya mengurangi produktivitas pertanian. Hal ini menunjukkan perlunya alternatif yang lebih berkelanjutan untuk menjaga kesehatan tanah dan meningkatkan hasil pertanian. Tujuan dari pelatihan ini adalah untuk meningkatkan pengetahuan dan keterampilan petani Desa Banaran dalam memanfaatkan limbah organik menjadi pupuk organik yaitu Mikroorganisme Lokal (MOL) yang lebih ramah lingkungan. Metode yang digunakan meliputi sosialisasi, pelatihan, dan evaluasi. Sosialisasi dilakukan melalui ceramah dan diskusi tentang MOL, sedangkan pelatihan melibatkan praktik langsung pembuatan MOL dengan bahan-bahan yang tersedia secara lokal. Hasilnya, peserta menunjukkan peningkatan pengetahuan dan keterampilan dalam membuat dan menggunakan MOL, serta peningkatan kesadaran tentang pentingnya penggunaan pupuk organik untuk menjaga keseimbangan ekosistem.
Pancreatic lipase inhibitory activity of butterfly pea flower (Clitoria ternatea) Kombucha Roka Aji, Oktira; Putri, Nida’a Fauziyyah; Putri , Diah Asta
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 2 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i2.35724

Abstract

Preventing the action of pancreatic lipase is believed to be an effective method for treating obesity. Pancreatic lipase inhibitor act by suppressing the activity of pancreatic lipase, leading to a decrease in lipid absorption. Kombucha is a traditional fermented drink believed to have numerous health benefits, including as anti-obese. It can be produced using a range of substrates, such as butterfly pea flowers. The aim of this research was to investigate the inhibiting activity of kombucha made from butterfly pea flowers towards pancreatic lipase. The fermentation parameters monitored in this study included changes in cell density (OD600), the dry weight of the kombucha mushroom, pH, reducing sugar content, and the % titratable acid. The total phenol and total flavonoid content were also analyzed before and after fermentation. The inhibitory effect on pancreatic lipase of butterfly pea flower kombucha was presented as an IC50 value. The findings indicated that as fermentation progressed, the pH level and amount of reducing sugar decreased while the % titratable acid, cell density (OD600), and dry weight of the kombucha mushroom increased. The phenol and flavonoid content of butterfly pea kombucha was found to be greater compared to that of butterfly pea infusion, with respective levels of 0.040 mg GAE/g and 0.017 mg QE/g. This study confirms that butterfly pea flower kombucha has the ability to inhibit pancreatic lipase in vitro with an IC50 value of 162.83 μg/mL. Compared to butterfly pea flower tea infusion, butterfly pea flower kombucha has been found to be more effective in inhibiting pancreatic lipase. Thus, butterfly pea flower kombucha might be a promising candidate for being developed as an agent for combating obesity.
PELATIHAN OPTIMASI IRIGASI SMART FARMING DI KALURAHAN SERUT, GEDANGSARI, GUNUNG KIDUL Adi, Yudi Ari; Salamah, Umi; Haryadi, Bagus; Roka Aji, Oktira; Zul Ardi, Subhan; Rossa Puteri Baharie, Sri
Jurnal Berdaya Mandiri Vol. 6 No. 2 (2024): JURNAL BERDAYA MANDIRI (JBM)
Publisher : Universitas PGRI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/jbm.v6i2.6145

Abstract

The banana commodity is one of the prioritized commodities in Gunung Kidul Regency. The Young Farmers' Studio and Youth Organization “Solid Berkarya” in Serut Village are trying to develop banana plants by managing critical land into productive land using smart-farming irrigation systems. The concept of smart farming includes using technology such as sensors, connected devices (IoT), data analytics, artificial intelligence, and agricultural management software to create an efficient farming system. The irrigation system needs to apply optimization of timing and intensity tailored to the needs of the plants. The geographical terraced land conditions require unique methods to maximize agricultural yields. In banana cultivation, geographical conditions, climate, soil conditions, and water availability are crucial, so the community's knowledge and skills in irrigation on critical land must be improved. Therefore, banana irrigation training is conducted in this community service, especially related to water availability optimization. In this training, the participant's level of knowledge is measured by conducting pre-tests and post-tests related to irrigation optimization materials. From the test results, it can be seen that there is an increase in community knowledge from 6.44 before training to 9.0 after training. Thus, there is an increase in knowledge by 53.4%. The level of community satisfaction with the implementation of this service activity is 96.43%. keywords: Serut Village, Optimization, Smart-farming, Irrigation
Effect of An Additional Amount of Fish and Fermentation Time on The Sensory Properties of The Lemea Aziz, Abdul; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.8261

Abstract

Lemea, a traditional fermented food of the Rejang people in Bengkulu, is made from young bamboo and fish. The fish commonly used in the making Lemea is Mozambique tilapia or Mujair (Oreochromis mossambicus). The fermentation process typically lasts between 3 to 7 days. This study aimed to assess the sensory characteristics, including taste, color, texture, and aroma, of Lemea, based on the preferences of panelists. The variations examined were fermentation duration, and the amount of Mujair fish added. The research followed a completely randomized design, incorporating three fermentation durations (24, 96, and 144 hours) and three fish quantities (250 grams, 500 grams, and 750 grams). Sensory evaluations were conducted on the fermented samples, encompassing taste, aroma, texture, and color. The data revealed that the most favored taste and texture of Lemea were achieved with 500 grams of fish and a fermentation duration of 144 hours (J2W3). Likewise, the preferred aroma and color were observed in the sample with 750 grams of fish and 24 hours of fermentation (J3W1). The data analysis indicated that the sample with 500 grams of fish and a fermentation duration of 144 hours (J2W3) exhibited the most favorable sensory characteristics (taste, aroma, texture, and color). Thus, the quantity of fish added and the duration of fermentation influenced the sensory attributes of Lemea.
Antagonistic Activities of Endophytic Fungi from Chili Pepper (Capsicum frutescens L.) Stems and Leaves against Colletotrichum sp. Puspitarini, Sofia; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9736

Abstract

Colletotrichum sp. is one of the type pathogenic fumgi that can cause antachnose diseases. One of the plants that can be attacked by antrachnose is cayenne pepper (Capsicum frutescens L.). This study attempts to determine the ability of endophytic fungi from the stems and leaves cayenne pepper (Capsicum frutescens L.). Methods: This study used dual culture method to determine the percentage of resistence to patogen Colletotrichum sp. and the area of growth endophytic fungi planted on PDA (Potato Dextrose Agar). Results:. There are 3 isolates from the stems and leaves of cayenne pepper that is JE-B4-C, JE-D5-C, dan JE-B1-C. Among all the isolates, the largest percentage of inhibition and growth area endophytic fungi were found in isolate JE-B4-C with each of 29,41% dan 58,04 cm2. Conclusions: Expected isolates JE-B4-C derived from the genus eupenicillium, while isolates JE-D5-C and JE-B1-C respectively derived from the genus Aspergillus and Neoscytalidium.
Macrofungi Diversity in Wisdom Park UGM, Yogyakarta Zahra, Safa Auli; Safitri, Salsabila; Nugraha, Farhan Satya; Hidayah, Hidayah; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9743

Abstract

Indonesia's warm and humid tropical climate provides an ideal setting for the flourishing of fungi. Fungi are pivotal in the intricate balance of nutrient cycles and soil fertility within forest ecosystems. They can be broadly categorized into two types: microscopic fungi, which are minuscule and require observation under a microscope, and macroscopic fungi, which can be identified based on visible morphological features without additional tools. Forests, with their limited sunlight and high humidity due to the dense canopy of trees, serve as fertile grounds for a myriad of fungal species. This research aims to analyze fungal diversity using the exploratory survey method, involving direct search and observation of macrofungi. The sampling was conducted in May 2023 within the Wisdom Park UGM area, covering an area of 3,762.12 m² with coordinates 7° 46'09"S 110°22'59"E. The findings revealed a commendable diversity in terms of shape and size. However, the color palette of fungi in the area exhibited limited variation, primarily consisting of subdued white and brown hues. Some identified fungal species include Ganoderma sp., Mycena sp., Trametes sp., Lepiota cristata, and Panaeolus fimicola. These fungi predominantly thrived on substrates such as trees, soil, leaf litter, and decaying wood.
Co-Authors Abdul Aziz Adz-Dzikir, Fatihatuljannah Aisyah, Iis Nisya Nur Al Farisi, Irsyad Ambar Pratiwi Andika Bayu Chandra Aninda Kumala Sari ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Bagus Haryadi Cahya Ningsih, Ayu Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani ERNAWATI ARIFIN GIRI-RACHMAN Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah IHSANAWATI IHSANAWATI Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Kurnia, Dhiffa Trisna Laila, Alfiatul Larasati Haliimah Roosyidah Lestari, Iva Dita MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mar'atul Azizah Merlia Rahma Tari Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nursyiva Irsalinda Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia Putri , Diah Asta Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Sri Wahyuni Sri Wahyuni Subhan Zul Ardi Sumbudi, Milliniawati Sutan Nur Chamida Tri Astuti Suwartiningsih, Nurul Suwartiningsih, Nurul Tri Puspita Sari Tufahati, Nadhira umi salamah Umi Salamah Vianingtyas, Naresh Wari Mega Wachid Eko Purwanto Wicaksono, Adelyanty Pangestuty Wulandari, Endah Retno Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan