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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia Jurnal Pendidikan Sains AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas
Oktira Roka Aji
Program Studi Biologi, FAST, Universitas Ahmad Dahlan
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Antibacterial Efficacy of Turmeric (Curcuma domestica) Rhizome Infusion Against Aeromonas hydrophila and Its Toxicity Azizah, Nur; Aji, Oktira Roka
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.16934

Abstract

Aeromonas hydrophila bacteria can cause Motile Aeromonas Septicemia (MAS), a disease that impacts freshwater fish. The turmeric rhizome contains numerous bioactive compounds that act as antibacterials and might be utilized to inhibit A. hydrophila. This research aimed to identify the phytochemicals in turmeric (Curcuma domestica) infusion, evaluate its antibacterial activity against A. hydrophila, and define the toxicity effect of turmeric rhizome infusion. The agar dilution method was used to measure antibacterial activity, whereas the Brine Shrimp Lethality Test (BSLT) was used to assess toxicity. The research confirmed the presence of phytochemicals such as tannins, alkaloids, flavonoids, phenols, and saponins in a turmeric rhizome infusion. According to the findings, the infusion from turmeric rhizome effectively inhibited the growth of A. hydrophila at concentrations between 750-1000 ppm. In toxicity tests, the LC50 value of the turmeric rhizome infusion against shrimp larvae was 381.18 ppm. Hence, the turmeric rhizome infusion has the potential to be further developed for the prevention and treatment of fish infected with A. hydrophila in freshwater fish farming.
Antibacterial Efficacy of Turmeric (Curcuma domestica) Rhizome Infusion Against Aeromonas hydrophila and Its Toxicity Azizah, Nur; Aji, Oktira Roka
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.16934

Abstract

Aeromonas hydrophila bacteria can cause Motile Aeromonas Septicemia (MAS), a disease that impacts freshwater fish. The turmeric rhizome contains numerous bioactive compounds that act as antibacterials and might be utilized to inhibit A. hydrophila. This research aimed to identify the phytochemicals in turmeric (Curcuma domestica) infusion, evaluate its antibacterial activity against A. hydrophila, and define the toxicity effect of turmeric rhizome infusion. The agar dilution method was used to measure antibacterial activity, whereas the Brine Shrimp Lethality Test (BSLT) was used to assess toxicity. The research confirmed the presence of phytochemicals such as tannins, alkaloids, flavonoids, phenols, and saponins in a turmeric rhizome infusion. According to the findings, the infusion from turmeric rhizome effectively inhibited the growth of A. hydrophila at concentrations between 750-1000 ppm. In toxicity tests, the LC50 value of the turmeric rhizome infusion against shrimp larvae was 381.18 ppm. Hence, the turmeric rhizome infusion has the potential to be further developed for the prevention and treatment of fish infected with A. hydrophila in freshwater fish farming.
Aktivitas Antibakteri Infusa Daun Mangga Bacang (Mangifera foetida) Terhadap Bakteri Aeromonas hydrophila Cahya Ningsih, Ayu; Roka Aji, Oktira
Indobiosains 2025: Volume 7 Nomor 2 Agustus 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i2.17917

Abstract

Aeromonas hydrophila is an infectious agent that triggers Motile Aeromonas Septicemia (MAS) in freshwater fish, including catfish, leading to significant economic losses in aquaculture. This study aims to evaluate the antibacterial activity of Mango Bacang (Mangifera foetida L.) leaf infusion against A. hydrophila and its effect on catfish mortality. The research methods included the preparation of the infusion, phytochemical screening, antibacterial activity testing, and assessing the infusion's impact on catfish mortality. Phytochemical screening revealed that Mango Bacang leaf infusion contains alkaloids, flavonoids, phenols, tannins, and saponins, contributing to its antibacterial properties. The well-diffusion technique used to examine antibacterial effects demonstrated that the infusion at concentrations of 5–100% produced significant inhibition zones against A. hydrophila, with strong activity observed at a 5% concentration (14.38 ± 1.67 mm). However, toxicity testing on catfish indicated increased mortality with higher infusion concentrations, reaching 50% at 5% concentration. These findings suggest that Mango Bacang leaf infusion has potential as a natural antibacterial agent to control A. hydrophila, but its use requires precise dosage management to ensure safety for catfish. Further studies are necessary to optimize effective and environmentally friendly concentrations and explore applications on a broader scale.
Pemberdayaan komunitas peternak lebah Kelulut di Terengganu Malaysia melalui pelatihan pembuatan website sebagai media promosi Irsalinda, Nursyiva; Aji, Oktira Roka; Pangaribuan, Imelda
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 8, No 4 (2025): November
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v8i4.2916

Abstract

Peternak lebah kelulut di Terengganu menghadapi permasalahan dalam memanfaatkan media digital secara profesional, di mana mereka selama ini hanya mengandalkan media sosial seperti TikTok tanpa memiliki platform yang terstruktur dan kredibel untuk promosi produk. Tujuan pelatihan ini adalah untuk meningkatkan kapasitas digital para peternak dengan membekali mereka keterampilan dasar dalam membuat dan mengelola website menggunakan Google Sites sebagai media pemasaran digital yang lebih efektif. Pelatihan dilaksanakan dalam tiga tahapan, yaitu persiapan materi dan identifikasi kebutuhan peserta, pelaksanaan pelatihan secara praktikal melalui demonstrasi dan pendampingan langsung, serta evaluasi melalui pre-test dan post-test. Hasil menunjukkan peningkatan signifikan dalam pemahaman dan keterampilan peserta, di mana seluruh peserta yang sebelumnya belum memahami cara membuat website kini mampu membuat, mengelola, dan mengintegrasikan website mereka secara mandiri dengan media sosial dan marketplace. Efektivitas pelatihan ini diukur melalui peningkatan skor post-test dibandingkan pre-test serta keberhasilan peserta dalam menghasilkan website sebagai produk akhir. Dengan demikian, pelatihan berbasis praktik langsung terbukti efektif dalam meningkatkan literasi digital peternak dan memperluas jangkauan serta kredibilitas pemasaran produk mereka.
Efektivitas Nanogel Ekstrak Daun Getih-Getihan (Rivina humilis) Sebagai Obat Penyembuhan Luka Robek pada Tikus Aji, Oktira Roka; Tufahati, Nadhira; Yuliani, Ririn Kholifatu; Ilma, Nur Malika; Salimah, Azizah Zahratus
Jurnal Sains dan Edukasi Sains Vol. 8 No. 2 (2025): Jurnal Sains dan Edukasi Sains
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/juses.v8i2p159-166

Abstract

Getih-getihan (Rivina humilis) merupakan tanaman liar yang sering ditemukan tumbuh di sekitar pekarangan rumah atau lahan kosong. Daun dari tanaman getih-getihan diketahui mengandung berbagai senyawa aktif, salah satunya adalah flavonoid yang memiliki sifat antiinflamasi sehingga sangat berpotensi dalam mempercepat proses penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efektivitas berbagai konsentrasi nanogel ekstrak daun getih-getihan dalam membantu proses penyembuhan luka robek pada hewan uji tikus (Rattus norvegicus). Metode dalam penelitian ini  yang digunakan untuk menguji efektivitas konsentrasi nanogel ekstrak daun getih-getihan yaitu dengan menggunakan analisis eksperimental. Analisis data dilakukan dengan pengujian ANOVA untuk melihat perbedaan yang signifikan, dan kemudian dilanjutkan dengan uji lanjutan dengan analisis Duncan-Multiple Range Test (DMRT) untuk melihat kelompok perlakuan yang paling efektif. Hasil pengujian efektivitas nanogel ekstrak daun getih-getihan dengan konsentrasi 2 %, 4%, dan 6% b/v pada tikus (Rattus norvegicus) menunjukkan nanogel yang paling efektif dalam penyembuhan luka adalah pada konsentrasi 6%. Karena pada hari ke 6 menunjikan bahwa kondisi bekas luka sayatan sudah tertutup sempurna. Dengan demikian, dapat disimpulkan bahwa nanogel ekstrak daun getih-getihan dengan konsentrasi 6% merupakan formulasi paling efektif dalam mempercepat penyembuhan luka robek. Hasil penelitian ini menunjukkan potensi besar tanaman liar untuk dapat dikembangkan menjadi produk obat yang berbasis herbal sehingga aman dan ramah lingkungan.
Antimicrobial Properties of Endophytic Fungal Culture Filtrates from Tinospora crispa Aji, Oktira Roka; Fitria, Karomah Indah; Vianingtyas, Naresh Wari Mega; Wicaksono, Adelyanty Pangestuty
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.635-640

Abstract

Endophytic fungi can produce bioactive compounds that are useful as antimicrobials. This study evaluates the antimicrobial potential of culture filtrate extracts derived from endophytic fungi isolated from the medicinal plant Tinospora crispa. Isolation was carried out from the roots, leaves, and stems of T. crispa, which were then identified based on the ITS gene. The culture filtrate was extracted using ethyl acetate and assessed for antimicrobial activity using the disc diffusion method against Escherichia coli, Staphylococcus aureus, and Candida albicans. A total of 3 endophytic fungal isolates were isolated and identified as Acrocalymma vagum, Diaporthe tulliensis, and Colletotrichum truncatum. The results showed that all culture filtrate extracts of the fungal endophyte isolates exhibited varying antimicrobial activity, with the highest antibacterial activity demonstrated by C. truncatum isolates against Escherichia coli and Staphylococcus aureus. The most significant anticandida activity was by D. tulliensis isolates. Endophytic fungi of medicinal plant T. crispa can be developed as a source of antimicrobial agents, especially to overcome the increasing antibiotics resistance.
Aktivitas Antibakteri Ekstrak Daun Rivina humilis L. Terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Yuliani, Ririn Kholifatu; Ilma, Nur Malika; Tufahati, Nadhira; Salimah, Azizah Zahratus; Aji, Oktira Roka
JST (Jurnal Sains dan Teknologi) Vol. 14 No. 1 (2025): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v14i1.93228

Abstract

Rivina humilis (getih-getihan) is a wild plant that contains bioactive compounds that have the can to be used as antibiotics. This study aims to determine the antibacterial activity of ethanol extract of getih-getihan leaves against Staphylococcus aureus (Gram positive) and Pseudomonas aeruginosa (Gram negative) bacteria through in vitro experimental research. The subject used in the study was getih-getihan (Rivina humilis) leaves which were fresh and good without any disease. Data collection methods were carried out through laboratory experiments including extraction, metabolic analysis using LC-HRMS, antibacterial testing using disc diffusion, MIC and MBC. Data were analyzed using ANOVA and Duncan's post-hoc test with a significant value of p<0.05. The results of ethanol extract of getih-getihan leaves showed flavonoids, phenols, triterpenoids, amino acids, fatty acids, and carboxylic acids. The extract is can to inhibit and kill both bacteria, which is indicated by the presence of an inhibition zone, a decrease in absorbance, and no colony growth on the test media. In conclusion, ethanol extract of getih-getihan leaves has antibacterial activity against S. aureus and P. aeruginosa. The implication is that ethanol extract of getih-getihan leaves has the potential to be developed as a natural antibacterial agent that supports alternative treatment against bacterial infections against S. aureus and P. aeruginosa.
Co-Authors Abdul Aziz Adz-Dzikir, Fatihatuljannah Aisyah, Iis Nisya Nur Al Farisi, Irsyad Ambar Pratiwi Andika Bayu Chandra Aninda Kumala Sari ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Bagus Haryadi Cahya Ningsih, Ayu Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani ERNAWATI ARIFIN GIRI-RACHMAN Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah IHSANAWATI IHSANAWATI Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Kurnia, Dhiffa Trisna Laila, Alfiatul Larasati Haliimah Roosyidah Lestari, Iva Dita MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mar'atul Azizah Merlia Rahma Tari Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nursyiva Irsalinda Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia Putri , Diah Asta Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Sri Wahyuni Sri Wahyuni Subhan Zul Ardi Sumbudi, Milliniawati Sutan Nur Chamida Tri Astuti Suwartiningsih, Nurul Suwartiningsih, Nurul Tri Puspita Sari Tufahati, Nadhira umi salamah Umi Salamah Vianingtyas, Naresh Wari Mega Wachid Eko Purwanto Wicaksono, Adelyanty Pangestuty Wulandari, Endah Retno Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan