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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Canrea Journal: Food Technology, Nutritions, and Culinary Journal Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Proceeding Biology Education Conference Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas NJCOM: Community Service Journal Biodiversitas Journal of Biological Diversity
Oktira Roka Aji
Program Studi Biologi, Fakultas Sains Dan Teknologi Terapan, Universitas Ahmad Dahlan
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Pelatihan pengendalian Organisme Pengganggu Tanaman (OPT) pada budidaya pisang di Kelurahan Serut Kabupaten Gunungkidul Aji, Oktira Roka; Adi, Yudi Ari; Salamah, Umi; Ardi, Subhan Zul; Haryadi, Bagus
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 7, No 4 (2024): November
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v7i4.2224

Abstract

Pertanian adalah sektor penting di Kabupaten Gunung Kidul, DIY, dengan budidaya pisang memiliki potensi ekonomi yang menjanjikan. Program pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan petani dalam pengendalian organisme pengganggu tanaman (OPT) secara ramah lingkungan. Metode yang digunakan mencakup pendataan masalah, persiapan materi, pelatihan, evaluasi, dan tindak lanjut. Pelatihan dilakukan untuk 18 petani muda dari Sanggar Tani Muda di Kelurahan Serut, meliputi teknik pengendalian OPT preventif, biologis, dan kimiawi. Hasil menunjukkan peningkatan pengetahuan signifikan dari pre-test ke post-test, serta umpan balik positif dari peserta. Dengan demikian, program ini efektif dalam meningkatkan pemahaman dan keterampilan peserta, mendorong praktik pertanian berkelanjutan, serta meningkatkan produksi dan kesejahteraannya.
PELATIHAN OPTIMASI IRIGASI SMART FARMING DI KALURAHAN SERUT, GEDANGSARI, GUNUNG KIDUL Adi, Yudi Ari; Salamah, Umi; Haryadi, Bagus; Roka Aji, Oktira; Zul Ardi, Subhan; Rossa Puteri Baharie, Sri
Jurnal Berdaya Mandiri Vol. 6 No. 2 (2024): JURNAL BERDAYA MANDIRI (JBM)
Publisher : Universitas PGRI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/jbm.v6i2.6145

Abstract

The banana commodity is one of the prioritized commodities in Gunung Kidul Regency. The Young Farmers' Studio and Youth Organization “Solid Berkarya” in Serut Village are trying to develop banana plants by managing critical land into productive land using smart-farming irrigation systems. The concept of smart farming includes using technology such as sensors, connected devices (IoT), data analytics, artificial intelligence, and agricultural management software to create an efficient farming system. The irrigation system needs to apply optimization of timing and intensity tailored to the needs of the plants. The geographical terraced land conditions require unique methods to maximize agricultural yields. In banana cultivation, geographical conditions, climate, soil conditions, and water availability are crucial, so the community's knowledge and skills in irrigation on critical land must be improved. Therefore, banana irrigation training is conducted in this community service, especially related to water availability optimization. In this training, the participant's level of knowledge is measured by conducting pre-tests and post-tests related to irrigation optimization materials. From the test results, it can be seen that there is an increase in community knowledge from 6.44 before training to 9.0 after training. Thus, there is an increase in knowledge by 53.4%. The level of community satisfaction with the implementation of this service activity is 96.43%. keywords: Serut Village, Optimization, Smart-farming, Irrigation
Virtual Biology Laboratory Training for High School Biology Teachers and Laboratory Technicians in Yogyakarta Aji, Oktira Roka; Pratiwi, Ambar; Suwartiningsih, Nurul; Astuti, Sutan Nur Chamida Tri; Irsalinda, Nursyiva
Jurnal Abdimas Vol. 28 No. 2 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/57867x61

Abstract

As the COVID-19 pandemic comes to an end, education has returned to normal with in-person learning. The good practices in online learning conducted during the pandemic can be maintained to support practical activities, for example, using a virtual laboratory. The limitations of laboratory facilities and the skills of educators and laboratory staff in developing practical materials have made Biology practicals minimal and monotonous. Based on discussions with partners, there is a main problem that needs to be addressed through this program, which is the limited laboratory facilities in terms of tools and materials, resulting in minimal implementation of Biology practical activities in schools. The objective of this community service is to enhance the knowledge, skills, and empowerment of Biology teachers at Muhammadiyah high schools in Yogyakarta City in conducting practicals using virtual labs. This community service method is carried out by training on Biology practical learning online using virtual labs. This program is conducted in several sessions, each lasting 200 minutes, from June to October. Each session consists of a pre-test, material delivery, Q&A discussions, and a post-test. It can be concluded that the community service activities ran smoothly and provided benefits in the form of increased knowledge and skills of participants in Biology practical learning using virtual labs.
Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli Aji, Oktira Roka; Azizah, Mar'atul
Journal of Natural Sciences and Mathematics Research Vol. 9 No. 2 (2023): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Actinomycetes form associations with plants through colonizing plant tissues (endophytes) or by residing in the soil around some plants' roots (rhizosphere). Actinomycetes are known to produce antibacterial compounds. This study aimed to investigate the antibacterial activity of actinomycetes associated with the clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli. Actinomycetes were isolated from clove plants and the rhizosphere, and their antimicrobial activity against Staphylococcus aureus and Escherichia coli was evaluated using the agar plug method, where the presence of transparent zones around 10-day-old actinomycete growth indicated inhibition of bacterial growth. Four isolates showed inhibitory effects against Staphylococcus aureus, while only three isolates, B.4, T.3, and T.4, demonstrated inhibitory activity against Escherichia coli, as indicated by the presence of inhibition zones. Isolate T.3 exhibited the highest inhibition zone of 8.5 mm against S. aureus, whereas B.4 displayed the highest inhibition zone of 7.7 mm against E. coli. In conclusion, the actinomycetes found in clove plants (Syzygium aromaticum) demonstrate antibacterial properties against Staphylococcus aureus and Escherichia coli, indicating their potential as antibacterial agents.
Antimicrobial Properties of Endophytic Fungal Culture Filtrates from Tinospora crispa Oktira Roka Aji; Karomah Indah Fitria; Naresh Wari Mega Vianingtyas; Adelyanty Pangestuty Wicaksono
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.635-640

Abstract

Endophytic fungi can produce bioactive compounds that are useful as antimicrobials. This study evaluates the antimicrobial potential of culture filtrate extracts derived from endophytic fungi isolated from the medicinal plant Tinospora crispa. Isolation was carried out from the roots, leaves, and stems of T. crispa, which were then identified based on the ITS gene. The culture filtrate was extracted using ethyl acetate and assessed for antimicrobial activity using the disc diffusion method against Escherichia coli, Staphylococcus aureus, and Candida albicans. A total of 3 endophytic fungal isolates were isolated and identified as Acrocalymma vagum, Diaporthe tulliensis, and Colletotrichum truncatum. The results showed that all culture filtrate extracts of the fungal endophyte isolates exhibited varying antimicrobial activity, with the highest antibacterial activity demonstrated by C. truncatum isolates against Escherichia coli and Staphylococcus aureus. The most significant anticandida activity was by D. tulliensis isolates. Endophytic fungi of medicinal plant T. crispa can be developed as a source of antimicrobial agents, especially to overcome the increasing antibiotics resistance.
Banana Endophytic Fungi Producing Antagonistic, Antioxidant, and Antibacterial Bioactive Metabolites Aji, Oktira Roka; Putri, Diah Asta; Yuliani, Ririn Kholifatu; Ilma, Nur Malika; Astuti, Sutan Nur Chamida Tri
Indonesian Journal of Applied Research (IJAR) Vol. 7 No. 1 (2026): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v7i1.721

Abstract

The increasing prevalence of antibiotic-resistant pathogens and the environmental hazards associated with synthetic agrochemicals have intensified the search for natural bioactive agents. This study isolated and identified endophytic fungi from banana (Musa spp.) and evaluated their antimicrobial and antioxidant activities. Endophytic fungi were isolated from leaf and stem tissues using a surface-sterilization method, followed by culture purification. Antagonistic activity was assessed through dual culture assays, while molecular identification was performed using ITS rDNA sequencing. Five isolates were obtained, and isolate J2 exhibited clear antifungal activity against Fusarium oxysporum. Sequence analysis showed that J2 is closely related to Talaromyces aculeatus. Fungal metabolites were extracted using methanol and ethyl acetate, and the extracts were tested for antioxidant activity (DPPH assay), total phenolic content, and antibacterial activity against Escherichia coli and Bacillus cereus. The methanol extract exhibited stronger antioxidant activity (IC₅₀: 3285.41 ± 80.06 ppm), while the ethyl acetate extract showed higher phenolic content (45.04 ± 9.02 mg GAE/g) and superior antibacterial effects. These results highlight T. aculeatus as a promising natural source of multifunctional bioactive compounds.
Pemberdayaan Ekonomi Peternak Madu Kelulut Terengganu, Malaysia melalui Pendampingan Analisis Penjualan Oktira Roka Aji; Nursyiva Irsalinda; Imelda Pangaribuan
Jurnal Pengabdian Masyarakat Indonesia Vol 5 No 3 (2025): JPMI - Juni 2025
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.3553

Abstract

Madu Kelulut merupakan produk lebah tanpa sengat yang memiliki nilai ekonomi tinggi, namun pengelolaan penjualannya masih menjadi tantangan bagi peternak. Pengelolaan penjualan hanya terbatas pada pencatatan manual pada buku catatan. Pemahaman terhadap analisis penjualan belum memadai, sehingga strategi untuk peningkatan penjualan masih belum mampu dilakukan. Pelatihan analisis penjualan ini bertujuan untuk membekali komunitas peternak lebah madu Kelulut di Terengganu, Malaysia, dengan keterampilan dasar dalam pencatatan dan analisis penjualan menggunakan perangkat lunak sederhana. Kegiatan ini dilaksanakan melalui metode ceramah, diskusi interaktif, dan praktik langsung. Hasil evaluasi menunjukkan peningkatan signifikan dalam pemahaman peserta, di mana sebelum pelatihan, seluruh peserta (100%) belum mengetahui konsep analisis penjualan, namun setelah pelatihan, semua peserta telah memahami dan menguasai materi yang diberikan. Selain itu, mayoritas peserta (86%) menilai metode yang diajarkan mudah diterapkan, dan seluruh peserta (100%) menganggap pelatihan ini sangat bermanfaat bagi usaha mereka. Tingginya antusiasme dan respons positif terhadap pelatihan menunjukkan efektivitas metode yang digunakan. Dengan meningkatnya keterampilan dalam analisis penjualan, peternak dapat mengelola usaha mereka secara lebih efisien, meningkatkan strategi pemasaran, serta mendukung pencapaian SDG 12 terkait konsumsi dan produksi yang berkelanjutan.
Penerapan Keselamatan dan Kesehatan Kerja pada Lahan Miring di Sanggar Tani Kalurahan Serut Gunung Kidulul Subhan Zul Ardi; Yudi Ari Adi; Umi Salamah; Arif Rahman; Oktira Roka Aji
NJCOM: Community Service Journal Vol. 2 No. 2 (2026): JULY
Publisher : RAM PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.20095148

Abstract

Penerapan K3 selama ini lebih terfokus pada sektor formal yang memiliki struktur organisasi dan belum terfokus ke sektor informal. Oleh karena itu, penting untuk melakukan kajian mengenai penerapan K3 di sektor pertanian informal sebagai upaya dalam meningkatkan kesadaran akan keselamatan dan kesehatan. Desa Serut adalah salah satu desa yang terletak di Kecamatan Gedangsari, Kabupaten Gunung kidul dengan mayoritas bekerja sebagai buruh tani. Tujuan dari pengabdian adalah implementasi K3 pada pembukaan lahan pisang oleh sanggar tani muda pemerintah kalurahan serut. Solusi yang ditawarkan adalah para petani di desa serut memahami dan mengimplementasikan keselamatan ketika bekerja termasuk kondisi di lahan. Mitra dalam kegiatan PKM adalah Sanggar tani muda, dan pemerintah kalurahan Serut. Hasil yang telah dicapai adalah peningkatan pengetahuan pada para petani di sanggar tani muda.
Enhancing antioxidant activity and flavor profile of buni fruit (Antidesma bunius) kombucha through optimized fermentation conditions Oktira Roka Aji; Trianik Widyaningrum; Putri Nabela; Chlara Jelviora; Nisa Ihsani
jurnal1 VOLUME 9 ISSUE 1, JUNE 2026
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v9i1.1341

Abstract

Kombucha, a fermented beverage, is a rich source of antioxidants and offers various health benefits. This study explored the potential of Buni fruit (Antidesma bunius) in kombucha production, evaluating its chemical, biological, and antioxidant properties and sensory perceptions of Buni Fruit Kombucha under different fermentation conditions, including sugar concentration (5% and 10%), incubation temperature (4°C and 30°C), and fermentation duration (7 and 14 days). The chemical analysis of Buni fruit kombucha revealed a consistent decrease in pH, a gradual decline in sugar concentration, and an increase in total titratable acidity, highlighting significant changes during fermentation. During the 14-day incubation period, the microbial population dynamics of kombucha showed a significant decrease in total plate count and yeast count, but an increase in beneficial lactic acid bacteria. A significant difference in antioxidant activity was observed, with the highest activity recorded at an IC50 value of 61.32 ± 1.24 µL/mL. Sensory evaluation revealed no significant difference in the colour of kombucha among treatments, but there are significant differences in taste and aroma. The optimal conditions for enhancing antioxidant activity and sensory properties were found to be a 5% sugar concentration and fermentation at 30°C for 7 days. GC-MS analysis revealed the presence of three major compound groups (carboxylic acids, alcohols, and esters). These findings suggest that Buni has a high potential for use in creating functional foods through fermentation, which can contribute to developing functional kombucha beverages using fruit substrates to enhance their benefits and flavour profile.
Fermentation Characteristics and Bioactive Properties of Kombucha Senna alexandrina Mill. Leaves Oktira Roka Aji; Diah Asta Putri; Alya Firli Refanza Putri; Nabela Dwi Wahyu Nurvi Fibriyanti; Siti Nurhasanah
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1329-1336

Abstract

Kombucha is a fermented beverage produced by a symbiotic culture of bacteria and yeast (SCOBY) and is widely recognized for its functional properties. This study investigated the fermentation characteristics, bioactive properties, antibacterial activity, ethanol content, and sensory attributes of kombucha produced from senna leaves (Senna alexandrina Mill.) during different fermentation periods (0, 4, 8, and 12 days). Physicochemical parameters including pH, optical density, SCOBY biomass, reducing sugar, and total titratable acidity were monitored, while bioactive properties were evaluated through total phenolic content and antioxidant activity (IC50). Antibacterial activity against Escherichia coli, ethanol content, and sensory characteristics were also analyzed. The results showed that fermentation significantly decreased pH while increasing total titratable acidity, optical density, and SCOBY biomass, indicating active microbial metabolism. Total phenolic content increased during fermentation from 96.80 to 119.56 µg GAE/mL, accompanied by a substantial improvement in antioxidant activity as indicated by decreasing IC50 values. Antibacterial activity against E. coli was absent in the unfermented sample but emerged after fermentation. Ethanol concentration increased gradually but remained very low (0.0141% at day 12), well below regulatory limits for non-alcoholic and halal beverages. Sensory evaluation showed that fermentation time influenced taste and aroma, while color remained relatively stable. Overall, the findings demonstrate that senna leaves can serve as a suitable herbal substrate for kombucha fermentation and contribute to the development of functional fermented beverages with enhanced bioactive properties.
Co-Authors Abdul Aziz Adelyanty Pangestuty Wicaksono Adz-Dzikir, Fatihatuljannah Aila Gema Safitri Aisyah Aulia Nugraha Aisyah, Iis Nisya Nur Al Farisi, Irsyad Alfiatul Laila Alya Firli Refanza Putri Ambar Pratiwi Andika Bayu Chandra Aninda Kumala Sari Anisa Nurul Huda Ardi, Subhan Zul Arif Rahman Arifa Nadya Nur Afifah ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Azizah, Mar'atul Cahya Ningsih, Ayu Chlara Jelviora Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani Ernawati A. Giri Rachman Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Haris Setiawan Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah Ihsanawati Ihsanawati Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Karomah Indah Fitria Kinasti Dwi Lestari Kurnia, Dhiffa Trisna Larasati Haliimah Roosyidah Lestari, Iva Dita Linda Pertiwi Maelita Ramdani Moeis MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mardiah, Irma Merlia Rahma Tari Muhammad Farid Maksum Nabela Dwi Wahyu Nurvi Fibriyanti Naresh Wari Mega Vianingtyas Nisa Ihsani Nisa Ihsani Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nursyiva Irsalinda Nursyiva Irsalinda Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia PUTRI NABELA Putri , Diah Asta Putri Nabela Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rara Asti Azizah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Siti Nurhasanah Sri Wahyuni Sri Wahyuni Subhan Zul Ardi Sumbudi, Milliniawati Suwartiningsih, Nurul Suwartiningsih, Nurul Tiwi Sartika Tri Puspita Sari TRIANIK WIDYANINGRUM Trianik Widyaningrum Tufahati, Nadhira Umi Salamah Umi Salamah Umu Kamilah Vibula Rosa Arumbina Wachid Eko Purwanto Wulandari, Endah Retno Yudi Ari Adi Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan