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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia Jurnal Pendidikan Sains AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas
Oktira Roka Aji
Program Studi Biologi, FAST, Universitas Ahmad Dahlan
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Antimicrobial Properties of Endophytic Fungal Culture Filtrates from Tinospora crispa Aji, Oktira Roka; Fitria, Karomah Indah; Vianingtyas, Naresh Wari Mega; Wicaksono, Adelyanty Pangestuty
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.635-640

Abstract

Endophytic fungi can produce bioactive compounds that are useful as antimicrobials. This study evaluates the antimicrobial potential of culture filtrate extracts derived from endophytic fungi isolated from the medicinal plant Tinospora crispa. Isolation was carried out from the roots, leaves, and stems of T. crispa, which were then identified based on the ITS gene. The culture filtrate was extracted using ethyl acetate and assessed for antimicrobial activity using the disc diffusion method against Escherichia coli, Staphylococcus aureus, and Candida albicans. A total of 3 endophytic fungal isolates were isolated and identified as Acrocalymma vagum, Diaporthe tulliensis, and Colletotrichum truncatum. The results showed that all culture filtrate extracts of the fungal endophyte isolates exhibited varying antimicrobial activity, with the highest antibacterial activity demonstrated by C. truncatum isolates against Escherichia coli and Staphylococcus aureus. The most significant anticandida activity was by D. tulliensis isolates. Endophytic fungi of medicinal plant T. crispa can be developed as a source of antimicrobial agents, especially to overcome the increasing antibiotics resistance.
Aktivitas Antibakteri Ekstrak Daun Rivina humilis L. Terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Yuliani, Ririn Kholifatu; Ilma, Nur Malika; Tufahati, Nadhira; Salimah, Azizah Zahratus; Aji, Oktira Roka
JST (Jurnal Sains dan Teknologi) Vol. 14 No. 1 (2025): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v14i1.93228

Abstract

Rivina humilis (getih-getihan) is a wild plant that contains bioactive compounds that have the can to be used as antibiotics. This study aims to determine the antibacterial activity of ethanol extract of getih-getihan leaves against Staphylococcus aureus (Gram positive) and Pseudomonas aeruginosa (Gram negative) bacteria through in vitro experimental research. The subject used in the study was getih-getihan (Rivina humilis) leaves which were fresh and good without any disease. Data collection methods were carried out through laboratory experiments including extraction, metabolic analysis using LC-HRMS, antibacterial testing using disc diffusion, MIC and MBC. Data were analyzed using ANOVA and Duncan's post-hoc test with a significant value of p<0.05. The results of ethanol extract of getih-getihan leaves showed flavonoids, phenols, triterpenoids, amino acids, fatty acids, and carboxylic acids. The extract is can to inhibit and kill both bacteria, which is indicated by the presence of an inhibition zone, a decrease in absorbance, and no colony growth on the test media. In conclusion, ethanol extract of getih-getihan leaves has antibacterial activity against S. aureus and P. aeruginosa. The implication is that ethanol extract of getih-getihan leaves has the potential to be developed as a natural antibacterial agent that supports alternative treatment against bacterial infections against S. aureus and P. aeruginosa.
Pelatihan Pembuatan Mikroorganisme Lokal Untuk Pupuk Organik Di Desa Banaran, Kulon Progo Aji, Oktira Roka; Pratiwi, Ambar
Jurnal Pengabdian Masyarakat Indonesia Vol 4 No 4 (2024): JPMI - Agustus 2024
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.2769

Abstract

Ketergantungan petani pada pupuk kimia sintetis telah menyebabkan kerusakan struktur tanah dan penurunan kesuburan, yang pada akhirnya mengurangi produktivitas pertanian. Hal ini menunjukkan perlunya alternatif yang lebih berkelanjutan untuk menjaga kesehatan tanah dan meningkatkan hasil pertanian. Tujuan dari pelatihan ini adalah untuk meningkatkan pengetahuan dan keterampilan petani Desa Banaran dalam memanfaatkan limbah organik menjadi pupuk organik yaitu Mikroorganisme Lokal (MOL) yang lebih ramah lingkungan. Metode yang digunakan meliputi sosialisasi, pelatihan, dan evaluasi. Sosialisasi dilakukan melalui ceramah dan diskusi tentang MOL, sedangkan pelatihan melibatkan praktik langsung pembuatan MOL dengan bahan-bahan yang tersedia secara lokal. Hasilnya, peserta menunjukkan peningkatan pengetahuan dan keterampilan dalam membuat dan menggunakan MOL, serta peningkatan kesadaran tentang pentingnya penggunaan pupuk organik untuk menjaga keseimbangan ekosistem.
Empowerment of Kelompok Wanita Tani (KWT) Amanah, Sidomulyo in Ecoprint Making Aji, Oktira Roka; Pratiwi, Ambar
International Journal of Research in Community Services Vol. 5 No. 4 (2024)
Publisher : Research Collaboration Community (Rescollacom)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijrcs.v5i4.723

Abstract

Sidomulyo Village, situated in Bantul Regency, Indonesia, is confronted with economic challenges, particularly among its predominantly female population who face limited educational and economic opportunities. Recognizing the village's abundant natural resources, this community service program facilitated by Kelompok Wanita Tani (KWT) Amanah aimed to empower local women through ecoprint batik making. The program focused on equipping participants with skills in ecoprint techniques using locally sourced plant materials. Through stages of socialization, training, mentoring, and evaluation, the participants learned to create vibrant fabric patterns without chemical dyes, promoting environmental sustainability and preserving local biodiversity. Positive feedback highlighted the program's effectiveness in enhancing participants' skills and knowledge, with a strong interest shown in further developing ecoprint skills and exploring entrepreneurial opportunities, including online marketing. This study underscores the transformative impact of community-based initiatives in promoting women's economic independence and sustainable development through traditional craft practices.
Pemberdayaan anggota Pimpinan Cabang 'Aisyiyah (PCA) Gamping dalam pengolahan limbah organik rumah tangga Aji, Oktira Roka; Pratiwi, Ambar; Suwartiningsih, Nurul
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 7, No 2 (2024): Mei
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v7i2.1970

Abstract

Sampah organik merupakan bahan biodegradable yang mudah terdegradasi oleh dekomposer. pembuangan sampah organik menghasilkan bau yang tidak sedap. Kecamatan Gamping terletak di Kabupaten Sleman, Yogyakarta. Seiring bertambahnya jumlah penduduk, volume sampah meningkat pesat. Jika tidak ada kesadaran pengelolaan sampah oleh masyarakat, penumpukan sampah akan menjadi masalah lingkungan. Tujuan program ini yaitu memberikan penyuluhan dan pelatihan bagi peserta tentang pengelolaan sampah rumah tangga, khususnya sampah organik. Evaluasi pengetahuan peserta dilakukan dengan menyebarkan kuesioner pada awal dan akhir pelatihan. Peserta program ini menunjukkan antusiasme yang tinggi. Program ini memberikan efek positif yang signifikan pada seluruh peserta. Hal ini terlihat dari adanya peningkatan pengetahuan dan keterampilan peserta dalam mengelola sampah organik menjadi pupuk cair dan kompos. Harapannya, melalui program ini, peserta dapat berperan sebagai agen edukasi, mendidik anggota keluarga dan orang lain di sekitar untuk turut serta dalam upaya pengelolaan sampah organik di rumah tangga.
Pelatihan pengendalian Organisme Pengganggu Tanaman (OPT) pada budidaya pisang di Kelurahan Serut Kabupaten Gunungkidul Aji, Oktira Roka; Adi, Yudi Ari; Salamah, Umi; Ardi, Subhan Zul; Haryadi, Bagus
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 7, No 4 (2024): November
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v7i4.2224

Abstract

Pertanian adalah sektor penting di Kabupaten Gunung Kidul, DIY, dengan budidaya pisang memiliki potensi ekonomi yang menjanjikan. Program pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan petani dalam pengendalian organisme pengganggu tanaman (OPT) secara ramah lingkungan. Metode yang digunakan mencakup pendataan masalah, persiapan materi, pelatihan, evaluasi, dan tindak lanjut. Pelatihan dilakukan untuk 18 petani muda dari Sanggar Tani Muda di Kelurahan Serut, meliputi teknik pengendalian OPT preventif, biologis, dan kimiawi. Hasil menunjukkan peningkatan pengetahuan signifikan dari pre-test ke post-test, serta umpan balik positif dari peserta. Dengan demikian, program ini efektif dalam meningkatkan pemahaman dan keterampilan peserta, mendorong praktik pertanian berkelanjutan, serta meningkatkan produksi dan kesejahteraannya.
PELATIHAN OPTIMASI IRIGASI SMART FARMING DI KALURAHAN SERUT, GEDANGSARI, GUNUNG KIDUL Adi, Yudi Ari; Salamah, Umi; Haryadi, Bagus; Roka Aji, Oktira; Zul Ardi, Subhan; Rossa Puteri Baharie, Sri
Jurnal Berdaya Mandiri Vol. 6 No. 2 (2024): JURNAL BERDAYA MANDIRI (JBM)
Publisher : Universitas PGRI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/jbm.v6i2.6145

Abstract

The banana commodity is one of the prioritized commodities in Gunung Kidul Regency. The Young Farmers' Studio and Youth Organization “Solid Berkarya” in Serut Village are trying to develop banana plants by managing critical land into productive land using smart-farming irrigation systems. The concept of smart farming includes using technology such as sensors, connected devices (IoT), data analytics, artificial intelligence, and agricultural management software to create an efficient farming system. The irrigation system needs to apply optimization of timing and intensity tailored to the needs of the plants. The geographical terraced land conditions require unique methods to maximize agricultural yields. In banana cultivation, geographical conditions, climate, soil conditions, and water availability are crucial, so the community's knowledge and skills in irrigation on critical land must be improved. Therefore, banana irrigation training is conducted in this community service, especially related to water availability optimization. In this training, the participant's level of knowledge is measured by conducting pre-tests and post-tests related to irrigation optimization materials. From the test results, it can be seen that there is an increase in community knowledge from 6.44 before training to 9.0 after training. Thus, there is an increase in knowledge by 53.4%. The level of community satisfaction with the implementation of this service activity is 96.43%. keywords: Serut Village, Optimization, Smart-farming, Irrigation
Virtual Biology Laboratory Training for High School Biology Teachers and Laboratory Technicians in Yogyakarta Aji, Oktira Roka; Pratiwi, Ambar; Suwartiningsih, Nurul; Astuti, Sutan Nur Chamida Tri; Irsalinda, Nursyiva
Jurnal Abdimas Vol. 28 No. 2 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/57867x61

Abstract

As the COVID-19 pandemic comes to an end, education has returned to normal with in-person learning. The good practices in online learning conducted during the pandemic can be maintained to support practical activities, for example, using a virtual laboratory. The limitations of laboratory facilities and the skills of educators and laboratory staff in developing practical materials have made Biology practicals minimal and monotonous. Based on discussions with partners, there is a main problem that needs to be addressed through this program, which is the limited laboratory facilities in terms of tools and materials, resulting in minimal implementation of Biology practical activities in schools. The objective of this community service is to enhance the knowledge, skills, and empowerment of Biology teachers at Muhammadiyah high schools in Yogyakarta City in conducting practicals using virtual labs. This community service method is carried out by training on Biology practical learning online using virtual labs. This program is conducted in several sessions, each lasting 200 minutes, from June to October. Each session consists of a pre-test, material delivery, Q&A discussions, and a post-test. It can be concluded that the community service activities ran smoothly and provided benefits in the form of increased knowledge and skills of participants in Biology practical learning using virtual labs.
Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli Aji, Oktira Roka; Azizah, Mar'atul
Journal of Natural Sciences and Mathematics Research Vol. 9 No. 2 (2023): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Actinomycetes form associations with plants through colonizing plant tissues (endophytes) or by residing in the soil around some plants' roots (rhizosphere). Actinomycetes are known to produce antibacterial compounds. This study aimed to investigate the antibacterial activity of actinomycetes associated with the clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli. Actinomycetes were isolated from clove plants and the rhizosphere, and their antimicrobial activity against Staphylococcus aureus and Escherichia coli was evaluated using the agar plug method, where the presence of transparent zones around 10-day-old actinomycete growth indicated inhibition of bacterial growth. Four isolates showed inhibitory effects against Staphylococcus aureus, while only three isolates, B.4, T.3, and T.4, demonstrated inhibitory activity against Escherichia coli, as indicated by the presence of inhibition zones. Isolate T.3 exhibited the highest inhibition zone of 8.5 mm against S. aureus, whereas B.4 displayed the highest inhibition zone of 7.7 mm against E. coli. In conclusion, the actinomycetes found in clove plants (Syzygium aromaticum) demonstrate antibacterial properties against Staphylococcus aureus and Escherichia coli, indicating their potential as antibacterial agents.
Comparison of Quality and Ethanol Content of Liquid and Powder Kombucha from Jasmine Tea (Jasminum sambac L.) Nisa Ihsani; Anisa Nurul Huda; Arifa Nadya Nur Afifah; Tiwi Sartika; Linda Pertiwi; Maelita Ramdani Moeis; Muhammad Farid Maksum; Aila Gema Safitri; Oktira Roka Aji
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.47357

Abstract

The fermentation process of kombucha has become a focus of halal product research due to its ethanol content. Processing kombucha into powder form can be an alternative to address the ethanol content in liquid kombucha, as it has undergone a drying process, making it a potential halal-certified functional drink. The objective of this study was to compare the quality of liquid and powdered kombucha. The research was conducted from March to September 2024 at the Biotechnology Laboratory, Muhammadiyah University of Bandung. The method used was the Complete Randomized Design (CRD) method. The parameters analyzed included ethanol content, total BAA (Bacterial Acetic Acid), pH, organic acid content, and sugar content. In the production of powdered kombucha, optimization of maltodextrin and gum arabic usage was also conducted to achieve the best solubility and taste. The best powdered kombucha was obtained with a maltodextrin-to-gum arabic ratio of 3:2. In the research, liquid kombucha contained ethanol at an average of 0.09%. In comparison, no ethanol was detected in the powdered kombucha (0%). Total BAA in powder decreased after the drying process (p<0.05). The pH value of kombucha in liquid form (4.4) also differed significantly from powdered (3.2) (p<0.05). However, the levels of organic acids and sugars in liquid and powdered kombucha did not differ significantly (p>0.05). The sugar content in liquid and powdered kombucha was lower than that in kombucha in general.
Co-Authors Abdul Aziz Adz-Dzikir, Fatihatuljannah Aila Gema Safitri Aisyah, Iis Nisya Nur Al Farisi, Irsyad Andika Bayu Chandra Aninda Kumala Sari Anisa Nurul Huda Ardi, Subhan Zul Arifa Nadya Nur Afifah ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Azizah, Mar'atul Cahya Ningsih, Ayu Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani ERNAWATI ARIFIN GIRI-RACHMAN Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah IHSANAWATI IHSANAWATI Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Kurnia, Dhiffa Trisna Laila, Alfiatul Larasati Haliimah Roosyidah Lestari, Iva Dita Linda Pertiwi MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mardiah, Irma Merlia Rahma Tari Muhammad Farid Maksum Nisa Ihsani Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia Putri , Diah Asta Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Sri Wahyuni Sri Wahyuni Sumbudi, Milliniawati Suwartiningsih, Nurul Suwartiningsih, Nurul Tiwi Sartika Tri Puspita Sari Tufahati, Nadhira Umi Salamah Vianingtyas, Naresh Wari Mega Wachid Eko Purwanto Wicaksono, Adelyanty Pangestuty Wulandari, Endah Retno Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan