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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia Jurnal Pendidikan Sains AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas

Oktira Roka Aji

Program Studi Biologi, FAST, Universitas Ahmad Dahlan

Author-ID : 363454

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Virtual Biology Laboratory Training for High School Biology Teachers and Laboratory Technicians in Yogyakarta Oktira Roka Aji; Ambar Pratiwi; Nurul Suwartiningsih; Sutan Nur Chamida Tri Astuti; Nursyiva Irsalinda
Jurnal Abdimas Vol. 28 No. 2 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/57867x61

As the COVID-19 pandemic comes to an end, education has returned to normal with in-person learning. The good practices in online learning conducted during the pandemic can be maintained to support practical activities, for example, using a virtual laboratory. The limitations of laboratory facilities and the skills of educators and laboratory staff in developing practical materials have made Biology practicals minimal and monotonous. Based on discussions with partners, there is a main problem that needs to be addressed through this program, which is the limited laboratory facilities in terms of tools and materials, resulting in minimal implementation of Biology practical activities in schools. The objective of this community service is to enhance the knowledge, skills, and empowerment of Biology teachers at Muhammadiyah high schools in Yogyakarta City in conducting practicals using virtual labs. This community service method is carried out by training on Biology practical learning online using virtual labs. This program is conducted in several sessions, each lasting 200 minutes, from June to October. Each session consists of a pre-test, material delivery, Q&A discussions, and a post-test. It can be concluded that the community service activities ran smoothly and provided benefits in the form of increased knowledge and skills of participants in Biology practical learning using virtual labs.

Antibacterial activity of actinomycetes associated with clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli Oktira Roka Aji; Mar'atul Azizah
Journal of Natural Sciences and Mathematics Research Vol. 9 No. 2 (2023): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2023.9.2.16702

Actinomycetes form associations with plants through colonizing plant tissues (endophytes) or by residing in the soil around some plants' roots (rhizosphere). Actinomycetes are known to produce antibacterial compounds. This study aimed to investigate the antibacterial activity of actinomycetes associated with the clove plant (Syzygium aromaticum) against Staphylococcus aureus and Escherichia coli. Actinomycetes were isolated from clove plants and the rhizosphere, and their antimicrobial activity against Staphylococcus aureus and Escherichia coli was evaluated using the agar plug method, where the presence of transparent zones around 10-day-old actinomycete growth indicated inhibition of bacterial growth. Four isolates showed inhibitory effects against Staphylococcus aureus, while only three isolates, B.4, T.3, and T.4, demonstrated inhibitory activity against Escherichia coli, as indicated by the presence of inhibition zones. Isolate T.3 exhibited the highest inhibition zone of 8.5 mm against S. aureus, whereas B.4 displayed the highest inhibition zone of 7.7 mm against E. coli. In conclusion, the actinomycetes found in clove plants (Syzygium aromaticum) demonstrate antibacterial properties against Staphylococcus aureus and Escherichia coli, indicating their potential as antibacterial agents.

Pemberdayaan Ekonomi Peternak Madu Kelulut Terengganu, Malaysia melalui Pendampingan Analisis Penjualan Aji, Oktira Roka; Irsalinda, Nursyiva; Pangaribuan, Imelda
Jurnal Pengabdian Masyarakat Indonesia Vol 5 No 3 (2025): JPMI - Juni 2025
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.3553

Madu Kelulut merupakan produk lebah tanpa sengat yang memiliki nilai ekonomi tinggi, namun pengelolaan penjualannya masih menjadi tantangan bagi peternak. Pengelolaan penjualan hanya terbatas pada pencatatan manual pada buku catatan. Pemahaman terhadap analisis penjualan belum memadai, sehingga strategi untuk peningkatan penjualan masih belum mampu dilakukan. Pelatihan analisis penjualan ini bertujuan untuk membekali komunitas peternak lebah madu Kelulut di Terengganu, Malaysia, dengan keterampilan dasar dalam pencatatan dan analisis penjualan menggunakan perangkat lunak sederhana. Kegiatan ini dilaksanakan melalui metode ceramah, diskusi interaktif, dan praktik langsung. Hasil evaluasi menunjukkan peningkatan signifikan dalam pemahaman peserta, di mana sebelum pelatihan, seluruh peserta (100%) belum mengetahui konsep analisis penjualan, namun setelah pelatihan, semua peserta telah memahami dan menguasai materi yang diberikan. Selain itu, mayoritas peserta (86%) menilai metode yang diajarkan mudah diterapkan, dan seluruh peserta (100%) menganggap pelatihan ini sangat bermanfaat bagi usaha mereka. Tingginya antusiasme dan respons positif terhadap pelatihan menunjukkan efektivitas metode yang digunakan. Dengan meningkatnya keterampilan dalam analisis penjualan, peternak dapat mengelola usaha mereka secara lebih efisien, meningkatkan strategi pemasaran, serta mendukung pencapaian SDG 12 terkait konsumsi dan produksi yang berkelanjutan.

Profil Resistensi Antibiotik Escherichia coli dari Peternakan Ayam di Bantul, Yogyakarta Wulandari, Endah Retno; Astuti, Sutan Nur Chamida Tri; Falih, Afifah Nurul; Aji, Oktira Roka
Filogeni: Jurnal Mahasiswa Biologi Vol 5 No 2 (2025): Mei-Agustus
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v5i2.57307

Resistensi antibiotik merupakan isu kesehatan penting yang berkaitan dengan penyebaran bakteri resisten dari peternakan ayam ke lingkungan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Escherichia coli dari perternakan ayam serta menentukan profil kepekaan antibiotiknya. Sampel diambil dari peternakan ayam di Bantul, Yogyakarta. Penelitian diawali dengan isolasi bakteri E. coli, diikuti pewarnaan Gram serta identifikasi molekuler dengan gen uspA. Uji sensitivitas antibiotik dilakukan menggunakan metode Kirby-Bauer terhadap antibiotik ampisilin, tetrasiklin dan kloramfenikol. Hasil menunjukkan bahwa 4 dari 8 isolat (A2, A4, B2, B3) merupakan positif bakteri E. coli, ditandai dengan koloni hijau metalik pada EMBA dan pita DNA sepanjang 884 bp pada hasil elektroforesis gen uspA. Uji resistensi menunjukkan seluruh isolat resisten terhadap ampisilin (100%), sedangkan terhadap tetrasiklin dan kloramfenikol menunjukkan hasil yang lebih rendah (50%). Temuan ini mengindikasikan adanya potensi penyebaran bakteri resisten dari peternakan ayam sehingga diperlukan pengawasan penggunaan antibiotik dan pemantauan resistensi secara berkala.

Growth Response and Biochemistry of Red Spinach (Amaranthus tricolor L.) with the Application of Liquid Organic Fertilizer Lemna sp. Pratiwi, Ambar; Roka Aji, Oktira; Sumbudi, Milliniawati
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Red spinach is widely cultivated and consumed by the public because it has high antioxidants, vitamins (A, C, E), protein, carbohydrates, fats, minerals, iron, magnesium, manganese, potassium and calcium. P giving POC can increasered spinach biomass. Nutrients in POC have been decomposed so that they are easily absorbed by plants. This study aims to analyze the giving of POC Lemna sp. on growth and biochemical content of red spinach and determine the concentration of POC Lemna sp. effective for red spinach (Amaranthus tricolor L.). The study used a completely randomized design with 3 replications and 3 treatments. The independent variable in this study was the concentration of liquid organic fertilizer Lemna sp. (A=0%, B=5%, and C=10%). Data were analyzed by ANOVA test and DMRT test at 5% level. The results showed that the administration of POC Lemna sp. gave significantly different results on the A (0%) treatment of plant height, stem diameter, and flavonoid parameters. Giving POC Lemna sp. can increase the growth and flavonoid content of red spinach. The concentration of liquid organic fertilizer Lemna sp. 5% effective for the growth of red spinach (Amaranthus tricolor L.).

Antibiotic Resistance in Escherichia coli Isolated from Chicken Meat in Traditional Markets of Yogyakarta Al Farisi, Irsyad; Astuti, Sutan Nur Chamida Tri; Falih, Afifah Nurul; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.13125

The widespread use of antibiotics in the livestock industry serves both as a preventive measure against diseases and as a feed additive. However, excessive antibiotic use has led to antibiotic resistance, where bacterial exposure to antibiotics renders them resistant to one or more antibiotic agents. This study investigates the presence of antibiotic resistance in Escherichia coli isolated from chicken meat sold in traditional markets in Yogyakarta. An experimental method was employed, using ECB and EMBA media for bacterial isolation. Gram staining was performed to characterize the morphology of E. coli, followed by molecular testing using the uspA gene. Antibiotic susceptibility testing was conducted using the Kirby-Bauer disk diffusion method. A total of 10 chicken meat samples were collected from five traditional markets in Yogyakarta. The antibiotics tested included ampicillin, ciprofloxacin, and chloramphenicol. The results indicated resistance in all 10 isolates. Sample codes G1 and G2 were resistant to all three antibiotics. Samples P1, P2, and L1 exhibited resistance to ampicillin and chloramphenicol, with intermediate resistance to ciprofloxacin. Sample L2 showed resistance to ampicillin, intermediate resistance to ciprofloxacin, and sensitivity to chloramphenicol. Sample B1 exhibited resistance to all antibiotics tested. Sample B2 was resistant to ampicillin and ciprofloxacin, with intermediate resistance to chloramphenicol. Samples K1 and K2 were resistant to ampicillin and chloramphenicol but sensitive to ciprofloxacin. These findings highlight the growing concern of antibiotic resistance in bacterial strains isolated from chicken meat, emphasizing the need for stricter regulations on antibiotic use in the poultry industry.

Pengendalian Parasit Trichodina sp. Menggunakan Infusa Daun Jambu Biji (Psidium guajava L.) pada Permukaan Kulit Ikan Lele (Clarias batrachus L.) Santrianda, Aldhiani; Aji, Oktira Roka
Biosel Biology Science and Education Vol. 10 No. 1 (2021): BIOSEL (Biology Science and Education: Jurnal Penelitian Science dan Pendidika
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Parasit adalah organisme yang hidup pada tubuh organisme lain dan umumnya menimbulkan dampak negatif pada organisme yang ditempatinya. Salah satu parasit yang kerap menyerang ikan lele dari golongan protozoa adalah Trichodina sp. yang menyebabkan gatal. Pengendalian penyakit Trichodinasis ini dapat dilakukan dengan menggunakan obat kimia, namun cara ini dapat mencemari lingkungan dan produk makanan. Oleh karena itu, penelitian ini dilakukan untuk penanggulangan parasit menggunakan bahan alami diantaranya dengan menggunakan infusa daun jambu biji. Daun jambu biji mengandung tanin, alkaloid, fenol dan saponin sebagai antiparasit. Penelitian ini bertujuan untuk mengetahui pengaruh infusa daun jambu biji terhadap penurunan prevalensi ikan lele yang terserang parasit Trichodina sp. dan intensitas parasit Trichodina sp. Variabel bebas dari penelitian ini adalah konsentrasi infusa daun jambu biji (Psidium guajava L.) yang digunakan, yaitu 2,5 mL/L; 5 mL/L; 10 mL/L dan kontrol (tanpa infusa daun jambu biji). Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan 5 kali ulangan sehingga diperoleh 20 unit percobaan. Tahapan penelitian meliputi infundasi daun jambu biji, penginfeksian parasit ke ikan, perendaman, dan pengamatan. Data yang diperoleh dianalisis menggunakan Analisis Varians (ANOVA) dan dilanjutkan uji Duncan jika terdapat beda nyata. Data ini dikomputerisasi menggunakan program SPSS. Berdasarkan hasil penelitian, pemberian infusa daun jambu biji memiliki pengaruh nyata terhadap prevalensi ikan lele yang terserang parasit Trichodina sp. dan intensitas parasit Trichodina sp., konsentrasi infusa daun jambu biji yang berbeda paling nyata adalah 10 mL/L.

KARAKTERISTIK DAN AKTIVITAS ANTIOKSIDAN KOMBUCHA MESOKARP MENTIMUN (Cucumis sativus L.) Kurnia, Dhiffa Trisna; Ilma, Nur Malika; Pinasti, Vanya Aurel; Putri, Aulia Ramanda; Aji, Oktira Roka
BIOTIKA Jurnal Ilmiah Biologi Vol 22, No 2 (2024): BIOTIKA DESEMBER 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v22i2.59897

Karakteristik kombucha terbagi menjadi karakteristik kimiawi dan biologi. Kombucha merupakan minuman fermentasi yang kaya akan kandungan antioksidan. Mentimun dipercaya bermanfaat sebagai sumber antioksidan yang dapat mencegah radikal bebas. Penelitian tentang aktivitas antioksidan kombucha dengan berbagai substrat sudah banyak dilakukan, tetapi penelitian tentang aktivitas antioksidan kombucha mesokarp mentimun (Cucumis sativus L.) belum pernah dilakukan. Oleh karena itu, penelitian ini dilakukan untuk mengetahui karakteristik serta aktivitas antioksidan kombucha mesokarp mentimun pada hari ke-6 dan 12. Fermentasi dilakukan selama 6 dan 12 hari, kemudian masing-masing sampel diuji karakteristiknya, seperti pH, berat kering scoby (Symbiotic Culture of Bacteria and Yeast), kadar gula pereduksi, serta total asam. Aktivitas antioksidan kombucha mesokarp mentimun diuji melalui metode DPPH, kemudian dihitung nilai IC50 nya. Hasil menunjukkan pH dan kadar gula pereduksi mengalami penurunan, sedangkan berat kering scoby dan total asam mengalami peningkatan. Aktivitas antioksidan kombucha mesokarp mentimun menunjukkan nilai IC50 hari ke-6 sebesar 3,5 ppm dan hari ke-12 sebesar 4,4 ppm. Kombucha mesokarp mentimun memiliki antioksidan sangat kuat yang dapat dimanfaatkan sebagai pengobatan alternatif.

Antagonistic and Antifungal Activities of Endophytic Fungi Isolated from Fragaria x Ananassa Against Colletotrichum Acutatum Aji, Oktira Roka; Laila, Alfiatul
Jurnal Internasional Teknik, Teknologi dan Ilmu Pengetahuan Alam Vol 7 No 1 (2025): International Journal of Engineering, Technology and Natural Sciences
Publisher : Universitas Teknologi Yogyakarta, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46923/ijets.v7i1.393

Fragaria x ananassa or strawberry has significant economic value worldwide. However, its cultivation faces various challenges. One example was fungal diseases, such as anthracnose, caused by Colletotrichum acutatum. Disease management generally relies heavily on synthetic fungicides, raising concerns about environmental sustainability and human health risks. Therefore, more environmentally friendly alternatives are needed, such as the use of biological agents. This study aims to assess the potential of endophytic fungi from strawberry plants as natural biocontrol agents against Colletotrichum acutatum. The study began with the isolation of endophytic fungi from strawberry tissues and their characterization morphologically. The antagonistic activity of endophytic fungi against colletotrichum acutatum was evaluated using three methods: a dual culture assay to assess direct inhibition, a cell-free filtrate assay to analyze the effect of fungal metabolites, and a volatile organic compound (VOC) assay to inhibit the growth of pathogenic pathogens. In this study, 20 fungal isolates were successfully isolated. A total of 15 showed antagonism against Colletotrichum acutatum. Isolate A1 showed potent inhibition in the dual culture test by effectively antagonizing Colletotrichum acutatum. Isolate D1 produced extracellular compounds that reduced the growth of Colletotrichum acutatum by 52.28% ± 3.21% in the cell-free filtrate assay. At the same time, isolate D3 proved most effective in the VOC assay, suppressing the growth of pathogenic colonies by 71.35% ± 3.37%. Thus, endophytic fungi, especially isolates A1, D1, and D3, have the potential to serve as an environmentally friendly alternative to combat anthracnose disease on strawberries.

Antagonistic Activity of the Endophy Fungi of Lime Plant (Citrus Aurantifolia) on Candida Albicans Aji, Oktira Roka; Wahyuni, Sri
Biosfer: Jurnal Tadris Biologi Vol 12 No 1 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i1.6367

This study aims to isolate and determine the antagonistic activity of lime's endophytic mushrooms on Candida albicans. Antagonism tests were performed in vitro with dual culture methods. The study obtained 14 isolates that were successfully isolated from the stem, roots, leaves, and peels of lime fruit. A total of 8 isolates could inhibit Candida albicans through antibiosis mechanisms and healthy competition. The isolates with the highest inhibition percentage were KD2 and MA1 (42.85%).

Co-Authors Abdul Aziz Adz-Dzikir, Fatihatuljannah Aisyah, Iis Nisya Nur Al Farisi, Irsyad Ambar Pratiwi Andika Bayu Chandra Aninda Kumala Sari ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Bagus Haryadi Cahya Ningsih, Ayu Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani ERNAWATI ARIFIN GIRI-RACHMAN Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah IHSANAWATI IHSANAWATI Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Kurnia, Dhiffa Trisna Laila, Alfiatul Larasati Haliimah Roosyidah Lestari, Iva Dita MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mar'atul Azizah Merlia Rahma Tari Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nursyiva Irsalinda Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia Putri , Diah Asta Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Sri Wahyuni Sri Wahyuni Subhan Zul Ardi Sumbudi, Milliniawati Sutan Nur Chamida Tri Astuti Suwartiningsih, Nurul Suwartiningsih, Nurul Tri Puspita Sari Tufahati, Nadhira umi salamah Umi Salamah Vianingtyas, Naresh Wari Mega Wachid Eko Purwanto Wicaksono, Adelyanty Pangestuty Wulandari, Endah Retno Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan

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