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All Journal Journal of Food and Pharmaceutical Science Indonesian Journal of Cancer Chemoprevention Prosiding Pendidikan Sains Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA BELIA: Early Childhood Education Papers Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Phinisi Integration Review Syntax Literate: Jurnal Ilmiah Indonesia Biosaintifika: Journal of Biology & Biology Education ASEAN Journal of Community Engagement Jurnal E-Bis: Ekonomi Bisnis Reswara: Jurnal Pengabdian Kepada Masyarakat Proceedings Series on Social Sciences & Humanities Journal Didaskalia Jurnal Akuntansi dan Keuangan Indonesia Al Iqtishadiyah: Journal of Islamic Economics and Finance Jurnal Natural Cendikia: Jurnal Pendidikan dan Pengajaran Journal of Food and Pharmaceutical Sciences JIFA (Journal of Islamic Finance and Accounting)
Rumiyati, Rumiyati
Department Of Pharmaceutical Chemistry, Faculty Of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Influence of fermented horse milk supplementation on immunity against hepatitis A vaccine in Balb/c mice Sasmito, Ediati; ., Rumiyati; W., Sri Rahayu; Andriyani, Evie; Istikharah, Rochmy
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 1, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.533 KB) | DOI: 10.14499/indonesianjpharm0iss0pp13-18

Abstract

It has been evaluated the influence of fermented horse milk into Balb/c mice on immunity against hepatitis A vaccine. The study employed a complete randomized design using thirty mice which were divided into 3 groups. Each group orally consumed fermented horse milk at dose 0.4 mL/20g body weight, levamisole (as positive control) at dose 0.4 mL/0.45 mg/20g body weight and distilled water (as negative control) at dose 0.4 mL/20g body weight respectively on a daily basis in addition to the standard diet for 46 days. The mice were immunized intraperitoneally (3 times) at d 7, d 28, and d 42, with 0.65 μg/20g body weight of hepatitis A vaccine. Blood samples were collected from plexus retroorbitalis using heparinized capillary one week after first (d 14), second (d 35) and 4 days (d 46) after third immunizations, and then mice were sacrificed to isolate the lymphocytes. The IgM, IgG and IgA titers in the sera and the culture media were measured by ELISA.The IgM (except d 46), IgG (subtype IgG1 (except d 14), IgG2a, IgG2b, IgG3 (except d 35), and IgA titers in the sera significantly higher (p<0.05) than controls. This difference was associated with enhanced in vitro antibodies secretion by cultured lymphocytes isolated from the spleen for IgA. The result suggested that orally fermented horse milk supplementation inhances immunity against hepatitis A vaccine in Balb/c mice. The SDS-PAGE showed that 30-kDa proteins were present in fermented horse milk as well as fermented cow milk, but 7.4-kDa protein present only in fermented horse milk.Key words : fermented horse milk, immunity, hepatitis A vaccine, Balb/c mice.
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL Ritmaleni, Ritmaleni; Arifin, Muhammad Fajar; Laksmiani, Ni Putu Linda; ., Rumiyati; ., Sismindari
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.791 KB) | DOI: 10.14499/indonesianjpharm0iss0pp9-17

Abstract

LR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione;  Leni  Ritmaleni  2),  which  designed  and  assumed  to  have biologically  activity  as  anticancer,  has  been  successfully synthesized  by  using  the  Biginelli  reaction.  This  research  was aimed  to  investigate  the  effect  of  benzaldehyde  excess  in  the synthesis  of  LR-2  and  to  evaluate  the  cytotoxic  activity  of  LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together  with thiourea at one time as  said  as  one  pot  reaction  synthetic  methodology  and  the reaction was acid catalysed. The mole equivalent of benzaldehyde was  in  excess  compare  to  others.  The  effect  of  benzaldehyde  in excess is the higher the mole of benzaldehyde, the lower the yield of  LR-2.  The  cytotoxicity  of  LR-2  was  done  by  using  MTT  method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa 
Apoptotic induction of ribosome-inactivating proteins (RIPs) isolated from Carica papaya L. leaves on cancer cell-lines ., Sismindari; ., Rumiyati; I.A., Ida Ayu Putu Indah; Enstini, Ni Luh Putu Yulina
Indonesian Journal of Pharmacy Vol 20 No 1, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.914 KB) | DOI: 10.14499/indonesianjpharm0iss0pp9-16

Abstract

Carica papaya L. leaves are potential for cancer treatment, this is due to the presence of Ribosome-inactivating proteins (RIPs), which demonstrated a cytotoxic effect on cancer cell lines. Therefore, this research was done to find out the effect of protein fraction containing RIPs isolated from C. papaya in the induction of apoptosis on cancer cell-lines (Raji and myeloma cell-lines).The protein fraction was isolated from Carica papaya L. leaves using ammonium sulphate precipitation and analyzed the activity on supercoiled double stranded DNA cleavage, in order to identify the presence of RIP. The active fraction called ‘CP protein’, was then analyzed on the induction of apoptosis by double staining method using acrydin orange-ethidium bromide, followed by analyzing the expressions of p53 and bcl2 using immunocytochemistry.The results demonstrated that the CP protein possessed cytotoxic activities on myeloma and Raji cell-lines with the IC50 of 1.80 mg/mL and 2.49 mg/mL respectively. Late apoptosis was detected on myeloma but not on Raji cell lines which was incubated with 0.9 mg/mL and 0.6 mg/mL of CP proteins respectively. In addition, the protein was able to increase the expression of apoptotic regulatory protein, p53, on myeloma cell-line and almost undetected on Raji cell-line.Key Words : Ribosome-inactivating proteins (RIPs), Carica papaya L. , expression of p53 and Bcl-2, myeloma cell-line, Raji cell-line.
Co-Authors ., Sismindari A.A. Ketut Agung Cahyawan W Abdul Rohman Adjara, Syafia Mustika Aisyah, Andi Nur Anif Nur Artanti, Anif Nur Annisa, RR Rifka Arifin, Muhammad Fajar Arin Pranesti Ariwibowo, Daniel Binar, Sri Burhan, Asril Dianila Oktyawati, Dianila Ediati Sasmito Edy Meiyanto Efi Miftah Faridli, Efi Miftah Endang Lukitaningsih Endang Purwati RN Endang Semiarti Enstini, Ni Luh Putu Yulina Evie Andriyani, Evie Fajri, Faridiah Aghadiati Frida Fanani Rohma Hofisah, Utbah Aminatul I.A., Ida Ayu Putu Indah Ika Puspita Sari Ika Puspitasari Indah Purwantini Irfan Muris Setiawan Juanda Juanda Khamidun, Khamidun Khamidun, Khamidun kusairi kusairi, kusairi Kusumanigrum, Aprilia Dyah Lilis Setyarini Mariyani MARLINI, WENNY Meti Christiana Meti Indrowati Mulia, Muhammad Adi Nabella Duta Nusa Nawwaruddin, Hazza Hammam Ni Putu Linda Laksmiani Nursenta Dahliana Purba Purnomo, Kardianto Indra Purwanto Purwanto Rahim, Kurnia Rarastoeti Pratiwi Rasyid, Rusman Rasyidah, Muthi'ah Riris Istighfari Jenie Ritmaleni, Ritmaleni Rochmy Istikharah RR. Ella Evrita Hestiandari Sari, Miftiana Nugraha Setiaji, Arkan Siregar, Hilda Octavana Sismindari . Sony Warsono Sophian, Alfi Sri Rahayu W., Sri Rahayu Syamsu Nur, Syamsu Widayanti, Agnisa Widianto, Kasiatin Zulfitria Zulfitria, Zulfitria